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Open data
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Basic information
Entry | Database: PDB / ID: 7qwk | |||||||||
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Title | GCN2 (EIF2ALPHA KINASE 4, E2AK4) IN COMPLEX WITH COMPOUND 2 | |||||||||
![]() | eIF-2-alpha kinase GCN2 | |||||||||
![]() | TRANSFERASE / GCN2 / EIF2 kinase / ISR / Integrated Stress Response | |||||||||
Function / homology | ![]() positive regulation of translational initiation in response to starvation / negative regulation of cytoplasmic translational initiation in response to stress / negative regulation of CREB transcription factor activity / regulation of translational initiation by eIF2 alpha phosphorylation / eiF2alpha phosphorylation in response to endoplasmic reticulum stress / GCN2-mediated signaling / eukaryotic translation initiation factor 2alpha kinase activity / negative regulation of translational initiation in response to stress / negative regulation by host of viral genome replication / regulation of feeding behavior ...positive regulation of translational initiation in response to starvation / negative regulation of cytoplasmic translational initiation in response to stress / negative regulation of CREB transcription factor activity / regulation of translational initiation by eIF2 alpha phosphorylation / eiF2alpha phosphorylation in response to endoplasmic reticulum stress / GCN2-mediated signaling / eukaryotic translation initiation factor 2alpha kinase activity / negative regulation of translational initiation in response to stress / negative regulation by host of viral genome replication / regulation of feeding behavior / T cell activation involved in immune response / positive regulation of adaptive immune response / regulation of translational initiation / cellular response to cold / neuron projection extension / Response of EIF2AK4 (GCN2) to amino acid deficiency / negative regulation of neuron differentiation / long-term memory / cytosolic ribosome / negative regulation of translational initiation / positive regulation of defense response to virus by host / cellular response to amino acid starvation / learning / DNA damage checkpoint signaling / positive regulation of long-term synaptic potentiation / cellular response to UV / defense response to virus / adaptive immune response / viral translation / protein autophosphorylation / tRNA binding / non-specific serine/threonine protein kinase / cell cycle / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / ATP binding / nucleus / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Maia de Oliveira, T. | |||||||||
Funding support | 1items
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![]() | ![]() Title: The structure of human GCN2 reveals a parallel, back-to-back kinase dimer with a plastic DFG activation loop motif. Authors: Maia de Oliveira, T. / Korboukh, V. / Caswell, S. / Winter Holt, J.J. / Lamb, M. / Hird, A.W. / Overman, R. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 413.5 KB | Display | ![]() |
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PDB format | ![]() | 330.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.3 MB | Display | ![]() |
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Full document | ![]() | 2.3 MB | Display | |
Data in XML | ![]() | 69 KB | Display | |
Data in CIF | ![]() | 92.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7qq6C ![]() 1zydS C: citing same article ( S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
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Components
#1: Protein | Mass: 36640.512 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q9P2K8, non-specific serine/threonine protein kinase #2: Chemical | ChemComp-G41 / ( #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 100mM Bis Tris, 20 to 30 percent PEG3350, 200mM ammonium sulfate PH range: 5.5-6.5 / Temp details: 2 |
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-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 15, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→42.67 Å / Num. obs: 90485 / % possible obs: 96.3 % / Redundancy: 1.8 % / Biso Wilson estimate: 44.58 Å2 / Rmerge(I) obs: 0.151 / Net I/σ(I): 3.6 |
Reflection shell | Resolution: 2.3→2.34 Å / Redundancy: 1.6 % / Rmerge(I) obs: 0.773 / Mean I/σ(I) obs: 0.9 / Num. unique obs: 3929 / % possible all: 85 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1ZYD Resolution: 2.3→42 Å / Cross valid method: THROUGHOUT
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Displacement parameters | Biso max: 147.42 Å2 / Biso mean: 50.4159 Å2 / Biso min: 18.87 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→42 Å
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