+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-7129 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
タイトル | Human PI4KIIIa lipid kinase complex | |||||||||
マップデータ | Human PI4KIIIa lipid kinase complex | |||||||||
試料 |
| |||||||||
キーワード | kinase / phosphoinositide synthesis / Transferase-Signaling Protein complex | |||||||||
機能・相同性 | 機能・相同性情報 : / Synthesis of PIPs at the ER membrane / 1-phosphatidylinositol 4-kinase / 1-phosphatidylinositol 4-kinase activity / viral replication complex formation and maintenance / Synthesis of PIPs at the Golgi membrane / phosphatidylinositol kinase activity / Golgi-associated vesicle membrane / phosphatidylinositol biosynthetic process / phosphatidylinositol-mediated signaling ...: / Synthesis of PIPs at the ER membrane / 1-phosphatidylinositol 4-kinase / 1-phosphatidylinositol 4-kinase activity / viral replication complex formation and maintenance / Synthesis of PIPs at the Golgi membrane / phosphatidylinositol kinase activity / Golgi-associated vesicle membrane / phosphatidylinositol biosynthetic process / phosphatidylinositol-mediated signaling / phosphatidylinositol phosphate biosynthetic process / myelination / protein localization to plasma membrane / kinase activity / phosphorylation / cadherin binding / neuron projection / viral RNA genome replication / focal adhesion / signal transduction / extracellular exosome / ATP binding / membrane / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.6 Å | |||||||||
データ登録者 | Lees JA / Zhang Y | |||||||||
資金援助 | 米国, 1件
| |||||||||
引用 | ジャーナル: Proc Natl Acad Sci U S A / 年: 2017 タイトル: Architecture of the human PI4KIIIα lipid kinase complex. 著者: Joshua A Lees / Yixiao Zhang / Michael S Oh / Curtis M Schauder / Xiaoling Yu / Jeremy M Baskin / Kerry Dobbs / Luigi D Notarangelo / Pietro De Camilli / Thomas Walz / Karin M Reinisch / 要旨: Plasma membrane (PM) phosphoinositides play essential roles in cell physiology, serving as both markers of membrane identity and signaling molecules central to the cell's interaction with its ...Plasma membrane (PM) phosphoinositides play essential roles in cell physiology, serving as both markers of membrane identity and signaling molecules central to the cell's interaction with its environment. The first step in PM phosphoinositide synthesis is the conversion of phosphatidylinositol (PI) to PI4P, the precursor of PI(4,5)P and PI(3,4,5)P This conversion is catalyzed by the PI4KIIIα complex, comprising a lipid kinase, PI4KIIIα, and two regulatory subunits, TTC7 and FAM126. We here report the structure of this complex at 3.6-Å resolution, determined by cryo-electron microscopy. The proteins form an obligate ∼700-kDa superassembly with a broad surface suitable for membrane interaction, toward which the kinase active sites are oriented. The structural complexity of the assembly highlights PI4P synthesis as a major regulatory junction in PM phosphoinositide homeostasis. Our studies provide a framework for further exploring the mechanisms underlying PM phosphoinositide regulation. | |||||||||
履歴 |
|
-構造の表示
ムービー |
ムービービューア |
---|---|
構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_7129.map.gz | 9.4 MB | EMDBマップデータ形式 | |
---|---|---|---|---|
ヘッダ (付随情報) | emd-7129-v30.xml emd-7129.xml | 24.9 KB 24.9 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_7129_fsc.xml | 11.2 KB | 表示 | FSCデータファイル |
画像 | emd_7129.png | 68 KB | ||
Filedesc metadata | emd-7129.cif.gz | 7.6 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-7129 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-7129 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_7129_validation.pdf.gz | 403.6 KB | 表示 | EMDB検証レポート |
---|---|---|---|---|
文書・詳細版 | emd_7129_full_validation.pdf.gz | 403.2 KB | 表示 | |
XML形式データ | emd_7129_validation.xml.gz | 12.2 KB | 表示 | |
CIF形式データ | emd_7129_validation.cif.gz | 16.2 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-7129 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-7129 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
---|---|
「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_7129.map.gz / 形式: CCP4 / 大きさ: 125 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
注釈 | Human PI4KIIIa lipid kinase complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.3 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
|
-添付データ
-試料の構成要素
-全体 : Human phosphatidylinositol 4-kinase III alpha complex
全体 | 名称: Human phosphatidylinositol 4-kinase III alpha complex |
---|---|
要素 |
|
-超分子 #1: Human phosphatidylinositol 4-kinase III alpha complex
超分子 | 名称: Human phosphatidylinositol 4-kinase III alpha complex タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#4 |
---|---|
由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 700 KDa |
-分子 #1: Phosphatidylinositol 4-kinase III alpha (PI4KA)
分子 | 名称: Phosphatidylinositol 4-kinase III alpha (PI4KA) / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO / EC番号: 1-phosphatidylinositol 4-kinase |
---|---|
由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 173.821438 KDa |
組換発現 | 生物種: Homo sapiens (ヒト) |
配列 | 文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)MMQCV IAVADKVFDA FLNMM ADKA KTKENEEELE RHAQFLLVNF NHIHKRIRRV ADKYLSGLVD KFPHLLWSGT VLKTMLDILQ TLSLSLSADI HKDQPY YDI PDAPYRITVP DTYEARESIV KDFAARCGMI LQEAMKWAPT VTKSHLQEYL NKHQNWVSGL SQHTGLAMAT ESILHFA GY NKQNTTLGAT QLSERPACVK KDYSNFMASL NLRNRYAGEV YGMIRFSGTT GQMSDLNKMM VQDLHSALDR SHPQHYTQ A MFKLTAMLIS SKDCDPQLLH HLCWGPLRMF NEHGMETALA CWEWLLAGKD GVEVPFMREM AGAWHMTVEQ KFGLFSAEI KEADPLAASE ASQPKPCPPE VTPHYIWIDF LVQRFEIAKY CSSDQVEIFS SLLQRSMSLN IGGAKGSMNR HVAAIGPRFK LLTLGLSLL HADVVPNATI RNVLREKIYS TAFDYFSCPP KFPTQGEKRL REDISIMIKF WTAMFSDKKY LTASQLVPPD N QDTRSNLD ITVGSRQQAT QGWINTYPLS SGMSTISKKS GMSKKTNRGS QLHKYYMKRR TLLLSLLATE IERLITWYNP LS APELELD QAGENSVANW RSKYISLSEK QWKDNVNLAW SISPYLAVQL PARFKNTEAI GNEVTRLVRL DPGAVSDVPE AIK FLVTWH TIDADAPELS HVLCWAPTDP PTGLSYFSSM YPPHPLTAQY GVKVLRSFPP DAILFYIPQI VQALRYDKMG YVRE YILWA ASKSQLLAHQ FIWNMKTNIY LDEEGHQKDP DIGDLLDQLV EEITGSLSGP AKDFYQREFD FFNKITNVSA IIKPY PKGD ERKKACLSAL SEVKVQPGCY LPSNPEAIVL DIDYKSGTPM QSAAKAPYLA KFKVKRCGVS ELEKEGLRCR SDSEDE CST QEADGQKISW QAAIFKVGDD CRQDMLALQI IDLFKNIFQL VGLDLFVFPY RVVATAPGCG VIECIPDCTS RDQLGRQ TD FGMYDYFTRQ YGDESTLAFQ QARYNFIRSM AAYSLLLFLL QIKDRHNGNI MLDKKGHIIH IDFGFMFESS PGGNLGWE P DIKLTDEMVM IMGGKMEATP FKWFMEMCVR GYLAVRPYMD AVVSLVTLML DTGLPCFRGQ TIKLLKHRFS PNMTEREAA NFIMKVIQSC FLSNRSRTYD MIQYYQNDIP Y UniProtKB: Phosphatidylinositol 4-kinase alpha |
-分子 #2: Tetratricopeptide repeat protein 7B
分子 | 名称: Tetratricopeptide repeat protein 7B / タイプ: protein_or_peptide / ID: 2 / コピー数: 2 / 光学異性体: LEVO |
---|---|
由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 96.46057 KDa |
組換発現 | 生物種: Homo sapiens (ヒト) |
配列 | 文字列: MYPYDVPDYA AIAGAPDLKL MATKKAGSRL ETEIERCRSE CQWERIPELV KQLSAKLIAN DDMAELLLGE SKLEQYLKEH PLRQGASPR GPKPQLTEVR KHLTAALDRG NLKSEFLQES NLIMAKLNYV EGDYKEALNI YARVGLDDLP LTAVPPYRLR V IAEAYATK ...文字列: MYPYDVPDYA AIAGAPDLKL MATKKAGSRL ETEIERCRSE CQWERIPELV KQLSAKLIAN DDMAELLLGE SKLEQYLKEH PLRQGASPR GPKPQLTEVR KHLTAALDRG NLKSEFLQES NLIMAKLNYV EGDYKEALNI YARVGLDDLP LTAVPPYRLR V IAEAYATK GLCLEKLPIS SSTSNLHVDR EQDVITCYEK AGDIALLYLQ EIERVILSNI QNRSPKPGPA PHDQELGFFL ET GLQRAHV LYFKNGNLTR GVGRFRELLR AVETRTTQNL RMTIARQLAE ILLRGMCEQS YWNPLEDPPC QSPLDDPLRK GAN TKTYTL TRRARVYSGE NIFCPQENTE EALLLLLISE SMANRDAVLS RIPEHKSDRL ISLQSASVVY DLLTIALGRR GQYE MLSEC LERAMKFAFE EFHLWYQFAL SLMAAGKSAR AVKVLKECIR LKPDDATIPL LAAKLCMGSL HWLEEAEKFA KTVVD VGEK TSEFKAKGYL ALGLTYSLQA TDASLRGMQE VLQRKALLAF QRAHSLSPTD HQAAFYLALQ LAISRQIPEA LGYVRQ ALQ LQGDDANSLH LLALLLSAQK HYHDALNIID MALSEYPENF ILLFSKVKLQ SLCRGPDEAL LTCKHMLQIW KSCYNLT NP SDSGRGSSLL DRTIADRRQL NTITLPDFSD PETGSVHATS VAASRVEQAL SEVASSLQSS APKQGPLHPW MTLAQIWL H AAEVYIGIGK PAEATACTQE AANLFPMSHN VLYMRGQIAE LRGSMDEARR WYEEALAISP THVKSMQRLA LILHQLGRY SLAEKILRDA VQVNSTAHEV WNGLGEVLQA QGNDAAATEC FLTALELEAS SPAVPFTIIP RVL UniProtKB: Tetratricopeptide repeat protein 7B |
-分子 #3: Protein FAM126A
分子 | 名称: Protein FAM126A / タイプ: protein_or_peptide / ID: 3 / コピー数: 2 / 光学異性体: LEVO |
---|---|
由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 32.688514 KDa |
組換発現 | 生物種: Homo sapiens (ヒト) |
配列 | 文字列: GPGSFTSEKG VVEEWLSEFK TLPETSLPNY ATNLKDKSSL VSSLYKVIQE PQSELLEPVC HQLFEFYRSG EEQLLQFTLQ FLPELIWCY LAVSASRNVH SSGCIEALLL GVYNLEIVDK QGHTKVLSFT IPSLSKPSVY HEPSSIGSMA LTESALSQHG L SKVVYSGP ...文字列: GPGSFTSEKG VVEEWLSEFK TLPETSLPNY ATNLKDKSSL VSSLYKVIQE PQSELLEPVC HQLFEFYRSG EEQLLQFTLQ FLPELIWCY LAVSASRNVH SSGCIEALLL GVYNLEIVDK QGHTKVLSFT IPSLSKPSVY HEPSSIGSMA LTESALSQHG L SKVVYSGP HPQREMLTAQ NRFEVLTFLL LCYNAALTYM PSVSLQSLCQ ICSRICVCGY PRQHVRKYKG ISSRIPVSSG FM VQMLTGI YFAFYNGEWD LAQKALDDII YRAQLELYPE PLLVANAIKA SLPHG UniProtKB: Hyccin |
-分子 #4: Phosphatidylinositol 4-kinase III alpha (PI4KA)
分子 | 名称: Phosphatidylinositol 4-kinase III alpha (PI4KA) / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO / EC番号: 1-phosphatidylinositol 4-kinase |
---|---|
由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 173.906547 KDa |
組換発現 | 生物種: Homo sapiens (ヒト) |
配列 | 文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)MMQC VIAVADKVFD A FLNMMADK AKTKENEEEL ERHAQFLLVN FNHIHKRIRR VADKYLSGLV DKFPHLLWSG TVLKTMLDIL QTLSLSLSAD IH KDQPYYD IPDAPYRITV PDTYEARESI VKDFAARCGM ILQEAMKWAP TVTKSHLQEY LNKHQNWVSG LSQHTGLAMA TES ILHFAG YNKQNTTLGA TQLSERPACV KKDYSNFMAS LNLRNRYAGE VYGMIRFSGT TGQMSDLNKM MVQDLHSALD RSHP QHYTQ AMFKLTAMLI SSKDCDPQLL HHLCWGPLRM FNEHGMETAL ACWEWLLAGK DGVEVPFMRE MAGAWHMTVE QKFGL FSAE IKEADPLAAS EASQPKPCPP EVTPHYIWID FLVQRFEIAK YCSSDQVEIF SSLLQRSMSL NIGGAKGSMN RHVAAI GPR FKLLTLGLSL LHADVVPNAT IRNVLREKIY STAFDYFSCP PKFPTQGEKR LREDISIMIK FWTAMFSDKK YLTASQL VP PDNQDTRSNL DITVGSRQQA TQGWINTYPL SSGMSTISKK SGMSKKTNRG SQLHKYYMKR RTLLLSLLAT EIERLITW Y NPLSAPELEL DQAGENSVAN WRSKYISLSE KQWKDNVNLA WSISPYLAVQ LPARFKNTEA IGNEVTRLVR LDPGAVSDV PEAIKFLVTW HTIDADAPEL SHVLCWAPTD PPTGLSYFSS MYPPHPLTAQ YGVKVLRSFP PDAILFYIPQ IVQALRYDKM GYVREYILW AASKSQLLAH QFIWNMKTNI YLDEEGHQKD PDIGDLLDQL VEEITGSLSG PAKDFYQREF DFFNKITNVS A IIKPYPKG DERKKACLSA LSEVKVQPGC YLPSNPEAIV LDIDYKSGTP MQSAAKAPYL AKFKVKRCGV SELEKEGLRC RS DSEDECS TQEADGQKIS WQAAIFKVGD DCRQDMLALQ IIDLFKNIFQ LVGLDLFVFP YRVVATAPGC GVIECIPDCT SRD QLGRQT DFGMYDYFTR QYGDESTLAF QQARYNFIRS MAAYSLLLFL LQIKDRHNGN IMLDKKGHII HIDFGFMFES SPGG NLGWE PDIKLTDEMV MIMGGKMEAT PFKWFMEMCV RGYLAVRPYM DAVVSLVTLM LDTGLPCFRG QTIKLLKHRF SPNMT EREA ANFIMKVIQS CFLSNRSRTY DMIQYYQNDI PY UniProtKB: Phosphatidylinositol 4-kinase alpha |
-分子 #5: 5-{2-amino-1-[4-(morpholin-4-yl)phenyl]-1H-benzimidazol-6-yl}-N-(...
分子 | 名称: 5-{2-amino-1-[4-(morpholin-4-yl)phenyl]-1H-benzimidazol-6-yl}-N-(2-fluorophenyl)-2-methoxypyridine-3-sulfonamide タイプ: ligand / ID: 5 / コピー数: 2 / 式: E4S |
---|---|
分子量 | 理論値: 574.626 Da |
Chemical component information | ChemComp-E4S: |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
---|---|
解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
濃度 | 0.25 mg/mL | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
緩衝液 | pH: 8 構成要素:
| |||||||||||||||
グリッド | モデル: Quantifoil R1.2/1.3 / 材質: COPPER / メッシュ: 400 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: HOLEY / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 30 sec. | |||||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 90 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
---|---|
撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: SUPER-RESOLUTION / デジタル化 - 画像ごとのフレーム数: 1-40 / 実像数: 3280 / 平均露光時間: 10.0 sec. / 平均電子線量: 47.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | C2レンズ絞り径: 100.0 µm / 最大 デフォーカス(補正後): 3.7 µm / 最小 デフォーカス(補正後): 1.8 µm / 倍率(補正後): 38461 / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 3.5 µm / 最小 デフォーカス(公称値): 2.0 µm / 倍率(公称値): 22500 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |