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Yorodumi- PDB-6drx: Structural Determinants of Activation and Biased Agonism at the 5... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6drx | |||||||||||||||||||||
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Title | Structural Determinants of Activation and Biased Agonism at the 5-HT2B Receptor | |||||||||||||||||||||
Components | 5HT2B receptor, BRIL chimera | |||||||||||||||||||||
Keywords | MEMBRANE PROTEIN / GPCR / 5HT2B / Setotonin receptor / Lisuride | |||||||||||||||||||||
Function / homology | Function and homology information intestine smooth muscle contraction / Gq/11-coupled serotonin receptor activity / phospholipase C-activating serotonin receptor signaling pathway / positive regulation of phosphatidylinositol biosynthetic process / G protein-coupled serotonin receptor signaling pathway / G protein-coupled serotonin receptor complex / protein kinase C signaling / regulation of behavior / Serotonin receptors / serotonin receptor signaling pathway ...intestine smooth muscle contraction / Gq/11-coupled serotonin receptor activity / phospholipase C-activating serotonin receptor signaling pathway / positive regulation of phosphatidylinositol biosynthetic process / G protein-coupled serotonin receptor signaling pathway / G protein-coupled serotonin receptor complex / protein kinase C signaling / regulation of behavior / Serotonin receptors / serotonin receptor signaling pathway / embryonic morphogenesis / cellular response to temperature stimulus / serotonin binding / vasoconstriction / G protein-coupled serotonin receptor activity / : / G protein-coupled receptor internalization / cardiac muscle hypertrophy / : / neural crest cell differentiation / neural crest cell migration / neurotransmitter receptor activity / cGMP-mediated signaling / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / positive regulation of cell division / G-protein alpha-subunit binding / heart morphogenesis / release of sequestered calcium ion into cytosol / positive regulation of endothelial cell proliferation / ERK1 and ERK2 cascade / phosphatidylinositol 3-kinase/protein kinase B signal transduction / phosphorylation / GTPase activator activity / positive regulation of MAP kinase activity / positive regulation of nitric-oxide synthase activity / positive regulation of cytokine production / electron transport chain / calcium-mediated signaling / intracellular calcium ion homeostasis / positive regulation of canonical NF-kappaB signal transduction / G alpha (q) signalling events / chemical synaptic transmission / positive regulation of ERK1 and ERK2 cascade / periplasmic space / electron transfer activity / response to xenobiotic stimulus / iron ion binding / G protein-coupled receptor signaling pathway / positive regulation of cell population proliferation / synapse / dendrite / heme binding / negative regulation of apoptotic process / nucleoplasm / plasma membrane / cytoplasm Similarity search - Function | |||||||||||||||||||||
Biological species | Homo sapiens (human) Escherichia coli (E. coli) | |||||||||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.1 Å | |||||||||||||||||||||
Authors | McCorvy, J.D. / Wacker, D. / Wang, S. / Agegnehu, B. / Liu, J. / Lansu, K. / Tribo, A.R. / Olsen, R.H.J. / Che, T. / Jin, J. / Roth, B.L. | |||||||||||||||||||||
Funding support | United States, 6items
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Citation | Journal: Nat. Struct. Mol. Biol. / Year: 2018 Title: Structural determinants of 5-HT2Breceptor activation and biased agonism. Authors: McCorvy, J.D. / Wacker, D. / Wang, S. / Agegnehu, B. / Liu, J. / Lansu, K. / Tribo, A.R. / Olsen, R.H.J. / Che, T. / Jin, J. / Roth, B.L. | |||||||||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6drx.cif.gz | 156.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6drx.ent.gz | 119.6 KB | Display | PDB format |
PDBx/mmJSON format | 6drx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dr/6drx ftp://data.pdbj.org/pub/pdb/validation_reports/dr/6drx | HTTPS FTP |
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-Related structure data
Related structure data | 6dryC 6drzC 6ds0C 4ib4S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 46049.887 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Escherichia coli (E. coli) Gene: HTR2B, cybC / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P41595, UniProt: P0ABE7 |
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#2: Chemical | ChemComp-H8G / |
#3: Chemical | ChemComp-CLR / |
#4: Chemical | ChemComp-OLC / ( |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.21 Å3/Da / Density % sol: 61.73 % |
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Crystal grow | Temperature: 293 K / Method: lipidic cubic phase Details: 100 mM Tris/HCl pH 7.4-7.7, 30-50 mM Ammonium tartrate dibasic, 30% v/v PEG400 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1.033 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Aug 4, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.033 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→30 Å / Num. obs: 10701 / % possible obs: 97.1 % / Redundancy: 4.4 % / CC1/2: 0.996 / Rmerge(I) obs: 0.122 / Net I/σ(I): 10.9 |
Reflection shell | Resolution: 3.1→3.17 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.95 / Mean I/σ(I) obs: 1.1 / Num. unique obs: 699 / CC1/2: 0.462 / % possible all: 97.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry: 4IB4 Resolution: 3.1→29.257 Å / SU ML: 0.5 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 28.99
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→29.257 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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