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Open data
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Basic information
Entry | Database: PDB / ID: 5dam | ||||||||||||||||||||||||||||
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Title | Crystal Structure of p-iodoHoechst bound to d(CGCAAATTTGCG) | ||||||||||||||||||||||||||||
![]() | DNA (5'-D(*![]() DNA / Minor groove binder DNA | Function / homology | p-iodo Hoechst / DNA / DNA (> 10) | ![]() Biological species | synthetic construct (others) | Method | ![]() ![]() ![]() Clark, G.R. | Funding support | | ![]()
![]() ![]() Title: Crystal Structure of p-iodoHoechst bound to d(CGCAAATTTGCG) Authors: Clark, G.R. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 28.9 KB | Display | ![]() |
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PDB format | ![]() | 17.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 3662.404 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #2: Chemical | ChemComp-58F / | #3: Chemical | ChemComp-MG / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 43.74 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: HANGING DROP, 19 DEG C |
-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Jan 25, 2001 / Details: OSMIC OPTICS |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54179 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→10 Å / Num. obs: 4783 / % possible obs: 94.2 % / Redundancy: 4.21 % / Rmerge(I) obs: 0.057 / Rsym value: 0.049 / Net I/σ(I): 22.17 |
Reflection shell | Resolution: 1.95→2.02 Å / Rmerge(I) obs: 0.226 / Mean I/σ(I) obs: 5.62 / Rsym value: 0.223 / % possible all: 91.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NDB ID GDL039 Resolution: 1.95→10 Å / Num. parameters: 2619 / Num. restraintsaints: 2589 / Cross valid method: FREE R-VALUE / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER
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Refine analyze | Num. disordered residues: 0 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 653.79 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→10 Å
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Refine LS restraints |
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