- PDB-4a82: Fitted model of staphylococcus aureus sav1866 model ABC transport... -
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データベース: PDB / ID: 4a82
タイトル
Fitted model of staphylococcus aureus sav1866 model ABC transporter in the human cystic fibrosis transmembrane conductance regulator volume map EMD-1966.
ジャーナル: J Biol Chem / 年: 2011 タイトル: The cystic fibrosis transmembrane conductance regulator (CFTR): three-dimensional structure and localization of a channel gate. 著者: Mark F Rosenberg / Liam P O'Ryan / Guy Hughes / Zhefeng Zhao / Luba A Aleksandrov / John R Riordan / Robert C Ford / 要旨: Cystic fibrosis affects about 1 in 2500 live births and involves loss of transmembrane chloride flux due to a lack of a membrane protein channel termed the cystic fibrosis transmembrane conductance ...Cystic fibrosis affects about 1 in 2500 live births and involves loss of transmembrane chloride flux due to a lack of a membrane protein channel termed the cystic fibrosis transmembrane conductance regulator (CFTR). We have studied CFTR structure by electron crystallography. The data were compared with existing structures of other ATP-binding cassette transporters. The protein was crystallized in the outward facing state and resembled the well characterized Sav1866 transporter. We identified regions in the CFTR map, not accounted for by Sav1866, which were potential locations for the regulatory region as well as the channel gate. In this analysis, we were aided by the fact that the unit cell was composed of two molecules not related by crystallographic symmetry. We also identified regions in the fitted Sav1866 model that were missing from the map, hence regions that were either disordered in CFTR or differently organized compared with Sav1866. Apart from the N and C termini, this indicated that in CFTR, the cytoplasmic end of transmembrane helix 5/11 and its associated loop could be partly disordered (or alternatively located).
THE FITTED MODEL IS DERIVED FROM THE PDB ENTRY 2HYD WHICH COMES FROM A BACTERIAL SOURCE ...THE FITTED MODEL IS DERIVED FROM THE PDB ENTRY 2HYD WHICH COMES FROM A BACTERIAL SOURCE (STAPHYLOCOCCUS AUREUS), BUT THE EXPERIMENTAL SOURCE FOR THE ELECTROM MICROSCOPY VOLUME MAP 1966 IS FROM HUMAN.
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実験情報
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実験
実験
手法: 電子線結晶学 / 使用した結晶の数: 140
EM実験
試料の集合状態: 2D ARRAY / 3次元再構成法: 電子線結晶学
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構成要素
名称: staphylococcus aureus sav1866 model ABC transporter in the human cystic fibrosis transmembrane conductance regulator タイプ: COMPLEX
解像度: 9→9 Å / σ(F): 2 詳細: SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-1966. (DEPOSITION ID: 10250). IN ORDER TO PROVIDE A DENSITY MAP FOR EMDB THAT THEY COULD HANDLE, AUTHORS DEPOSITED A MAP WHICH HAD BEEN ...詳細: SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-1966. (DEPOSITION ID: 10250). IN ORDER TO PROVIDE A DENSITY MAP FOR EMDB THAT THEY COULD HANDLE, AUTHORS DEPOSITED A MAP WHICH HAD BEEN EXTENDED ALONG A AND B AND THEN CROPPED TO CONTAIN ROUGHLY 2 UNIT CELLS. HENCE THE DIMENSIONS 140.7, 158.84, 247.5 REPRESENT ROUGHLY (NOT EXACTLY) 2 UNIT CELLS. IN ELECTRON CRYSTALLOGRAPHY THE C DIMENSION IS SET TO BE LARGER THA THE EXPECTED THICKNESS OF THE CRYSTAL (IN THIS CASE 300 ANGSTROM WA USED) AS THE CRYSTAL IS A SINGLE UNIT CELL THICK. THE THICKNESS OF 247.5 ANGSTROM ENCLOSES THE SIGNIFICANT DENSITY IN THE MAP WITH A FEW ANGSTROMS TO SPARE ON EACH SIDE OF THE CRYSTAL.