+Open data
-Basic information
Entry | Database: PDB / ID: 4u1b | ||||||
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Title | HsMetAP in complex with (1-amino-2-propylpentyl)phosphonic acid | ||||||
Components | Methionine aminopeptidase 1 | ||||||
Keywords | HYDROLASE | ||||||
Function / homology | Function and homology information N-terminal protein amino acid modification / peptidyl-methionine modification / initiator methionyl aminopeptidase activity / methionyl aminopeptidase / metalloexopeptidase activity / metalloaminopeptidase activity / protein maturation / aminopeptidase activity / cytosolic ribosome / platelet aggregation ...N-terminal protein amino acid modification / peptidyl-methionine modification / initiator methionyl aminopeptidase activity / methionyl aminopeptidase / metalloexopeptidase activity / metalloaminopeptidase activity / protein maturation / aminopeptidase activity / cytosolic ribosome / platelet aggregation / Inactivation, recovery and regulation of the phototransduction cascade / regulation of translation / proteolysis / metal ion binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.89 Å | ||||||
Authors | Arya, T. / Addlagatta, A. | ||||||
Citation | Journal: J.Med.Chem. / Year: 2015 Title: Identification of the Molecular Basis of Inhibitor Selectivity between the Human and Streptococcal Type I Methionine Aminopeptidases Authors: Arya, T. / Reddi, R. / Kishor, C. / Ganji, R.J. / Bhukya, S. / Gumpena, R. / McGowan, S. / Drag, M. / Addlagatta, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4u1b.cif.gz | 78.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4u1b.ent.gz | 57 KB | Display | PDB format |
PDBx/mmJSON format | 4u1b.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4u1b_validation.pdf.gz | 452.7 KB | Display | wwPDB validaton report |
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Full document | 4u1b_full_validation.pdf.gz | 454.4 KB | Display | |
Data in XML | 4u1b_validation.xml.gz | 15.4 KB | Display | |
Data in CIF | 4u1b_validation.cif.gz | 21.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u1/4u1b ftp://data.pdbj.org/pub/pdb/validation_reports/u1/4u1b | HTTPS FTP |
-Related structure data
Related structure data | 4u69C 4u6cC 4u6eC 4u6jC 4u6wC 4u6zC 4u70C 4u71C 4u73C 4u75C 4u76C C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 34454.207 Da / Num. of mol.: 1 / Fragment: UNP residues 81-386 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: METAP1, KIAA0094 / Production host: Escherichia coli (E. coli) / References: UniProt: P53582, methionyl aminopeptidase | ||||||
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#2: Chemical | #3: Chemical | ChemComp-GOL / | #4: Chemical | ChemComp-Q08 / [( | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.29 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6 Details: PEG 2000, potassium chloride, hepes, sodium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K PH range: 5.6 - 6.4 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1.54 Å |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Mar 20, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.89→22.53 Å / Num. obs: 26171 / % possible obs: 98 % / Redundancy: 3.6 % / Net I/σ(I): 3.16 |
-Processing
Software |
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Refinement | Resolution: 1.89→20 Å / SU B: 3.054 / SU ML: 0.09 / Cross valid method: THROUGHOUT / ESU R: 0.139 / ESU R Free: 0.139 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Displacement parameters | Biso mean: 33.14 Å2
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Refinement step | Cycle: LAST / Resolution: 1.89→20 Å
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