Nitric oxide stimulates guanylate cyclase / negative regulation of hepatic stellate cell contraction / synaptic signaling by nitric oxide / negative regulation of iron ion transmembrane transport / ROS and RNS production in phagocytes / azurophil granule / negative regulation of vasoconstriction / retrograde trans-synaptic signaling by nitric oxide / positive regulation of sodium ion transmembrane transport / response to nitric oxide ...Nitric oxide stimulates guanylate cyclase / negative regulation of hepatic stellate cell contraction / synaptic signaling by nitric oxide / negative regulation of iron ion transmembrane transport / ROS and RNS production in phagocytes / azurophil granule / negative regulation of vasoconstriction / retrograde trans-synaptic signaling by nitric oxide / positive regulation of sodium ion transmembrane transport / response to nitric oxide / Ion homeostasis / nitric oxide metabolic process / postsynaptic specialization, intracellular component / positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / negative regulation of cytosolic calcium ion concentration / peptidyl-cysteine S-nitrosylation / cadmium ion binding / behavioral response to cocaine / positive regulation of the force of heart contraction / negative regulation of potassium ion transport / calyx of Held / negative regulation of calcium ion transport / negative regulation of serotonin uptake / regulation of neurogenesis / sodium channel regulator activity / negative regulation of insulin secretion / response to vitamin E / nitric-oxide synthase (NADPH) / regulation of postsynaptic membrane potential / postsynaptic density, intracellular component / multicellular organismal response to stress / nitric-oxide synthase activity / xenobiotic catabolic process / negative regulation of peptidyl-serine phosphorylation / NADPH binding / arginine catabolic process / striated muscle contraction / regulation of sodium ion transport / nitric oxide mediated signal transduction / T-tubule / sarcoplasmic reticulum membrane / response to organonitrogen compound / cellular response to epinephrine stimulus / nitric oxide biosynthetic process / negative regulation of blood pressure / photoreceptor inner segment / response to nutrient levels / sarcoplasmic reticulum / secretory granule / response to activity / response to hormone / positive regulation of long-term synaptic potentiation / female pregnancy / muscle contraction / establishment of localization in cell / cell periphery / phosphoprotein binding / response to lead ion / response to nicotine / establishment of protein localization / potassium ion transport / response to organic cyclic compound / sarcolemma / cellular response to growth factor stimulus / response to peptide hormone / Z disc / cellular response to mechanical stimulus / response to estrogen / vasodilation / calcium ion transport / calcium-dependent protein binding / FMN binding / positive regulation of peptidyl-serine phosphorylation / NADP binding / flavin adenine dinucleotide binding / ATPase binding / response to heat / scaffold protein binding / nuclear membrane / response to ethanol / mitochondrial outer membrane / negative regulation of neuron apoptotic process / transmembrane transporter binding / response to lipopolysaccharide / dendritic spine / postsynaptic density / cytoskeleton / calmodulin binding / response to hypoxia / membrane raft / negative regulation of cell population proliferation / glutamatergic synapse / synapse / dendrite / heme binding / negative regulation of apoptotic process / perinuclear region of cytoplasm / positive regulation of DNA-templated transcription / enzyme binding / positive regulation of transcription by RNA polymerase II Similarity search - Function
NITRICOXIDESYNTHASE, BRAIN / / BNOS / CONSTITUTIVE NOS / NC-NOS / NOS TYPE I / NEURONAL NOS / N-NOS / NNOS / PEPTIDYL-CYSTEINE S- ...BNOS / CONSTITUTIVE NOS / NC-NOS / NOS TYPE I / NEURONAL NOS / N-NOS / NNOS / PEPTIDYL-CYSTEINE S-NITROSYLASE NOS1 / NEURONAL NITRIC OXIDE SYNTHASE
Mass: 48812.527 Da / Num. of mol.: 2 / Fragment: HEME DOMAIN, RESIDUES 297-718 Source method: isolated from a genetically manipulated source Source: (gene. exp.) RATTUS NORVEGICUS (Norway rat) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P29476, nitric-oxide synthase (NADPH)
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Non-polymers , 6 types, 330 molecules
#2: Chemical
ChemComp-HEM / PROTOPORPHYRINIXCONTAININGFE / HEME / Heme B
Mass: 616.487 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C34H32FeN4O4
Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1 Å / Relative weight: 1
Reflection
Resolution: 1.95→50 Å / Num. obs: 70257 / % possible obs: 99.3 % / Observed criterion σ(I): -3 / Redundancy: 5.1 % / Biso Wilson estimate: 33 Å2 / Rmerge(I) obs: 0.12 / Net I/σ(I): 10.4
Reflection shell
Resolution: 1.95→2.02 Å / Redundancy: 0.7 % / Rmerge(I) obs: 1.5 / Mean I/σ(I) obs: 0.7 / % possible all: 92.2
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Processing
Software
Name
Version
Classification
PHENIX
(PHENIX.REFINE)
refinement
XDS
datareduction
Aimless
datascaling
REFMAC
phasing
Refinement
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.951→37.996 Å / SU ML: 0.31 / σ(F): 1.33 / Phase error: 32.67 / Stereochemistry target values: ML Details: RESIDUES 339 TO 349 IN CHAIN A AND 339 TO 347 IN CHAIN B ARE DISORDERED.
Rfactor
Num. reflection
% reflection
Rfree
0.2413
3427
4.9 %
Rwork
0.1987
-
-
obs
0.2008
69300
98.12 %
Solvent computation
Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement step
Cycle: LAST / Resolution: 1.951→37.996 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
6659
0
175
321
7155
Refine LS restraints
Refine-ID
Type
Dev ideal
Number
X-RAY DIFFRACTION
f_bond_d
0.007
7067
X-RAY DIFFRACTION
f_angle_d
1.112
9622
X-RAY DIFFRACTION
f_dihedral_angle_d
15.395
2572
X-RAY DIFFRACTION
f_chiral_restr
0.074
994
X-RAY DIFFRACTION
f_plane_restr
0.005
1217
LS refinement shell
Resolution (Å)
Rfactor Rfree
Num. reflection Rfree
Rfactor Rwork
Num. reflection Rwork
Refine-ID
% reflection obs (%)
1.9506-1.9785
0.4537
128
0.4231
2039
X-RAY DIFFRACTION
75
1.9785-2.008
0.4132
131
0.4044
2571
X-RAY DIFFRACTION
93
2.008-2.0394
0.4206
129
0.3742
2683
X-RAY DIFFRACTION
97
2.0394-2.0728
0.4222
140
0.3681
2723
X-RAY DIFFRACTION
98
2.0728-2.1086
0.4009
145
0.3504
2705
X-RAY DIFFRACTION
99
2.1086-2.1469
0.4103
113
0.3293
2799
X-RAY DIFFRACTION
99
2.1469-2.1882
0.4156
133
0.3107
2749
X-RAY DIFFRACTION
99
2.1882-2.2329
0.4181
139
0.2927
2748
X-RAY DIFFRACTION
99
2.2329-2.2814
0.3064
159
0.2746
2755
X-RAY DIFFRACTION
100
2.2814-2.3345
0.3251
160
0.247
2737
X-RAY DIFFRACTION
100
2.3345-2.3928
0.2851
138
0.2451
2753
X-RAY DIFFRACTION
100
2.3928-2.4575
0.3093
141
0.2415
2811
X-RAY DIFFRACTION
100
2.4575-2.5298
0.3289
142
0.233
2738
X-RAY DIFFRACTION
100
2.5298-2.6115
0.2866
149
0.2211
2791
X-RAY DIFFRACTION
100
2.6115-2.7048
0.2874
131
0.2206
2784
X-RAY DIFFRACTION
100
2.7048-2.813
0.262
135
0.2117
2785
X-RAY DIFFRACTION
100
2.813-2.941
0.2436
144
0.2094
2797
X-RAY DIFFRACTION
100
2.941-3.096
0.2383
163
0.2098
2788
X-RAY DIFFRACTION
100
3.096-3.2899
0.2647
141
0.2015
2798
X-RAY DIFFRACTION
100
3.2899-3.5437
0.2217
141
0.1749
2818
X-RAY DIFFRACTION
100
3.5437-3.9
0.2052
161
0.1517
2798
X-RAY DIFFRACTION
100
3.9-4.4636
0.1544
146
0.1329
2846
X-RAY DIFFRACTION
99
4.4636-5.6208
0.1753
166
0.1304
2852
X-RAY DIFFRACTION
99
5.6208-38.003
0.1621
152
0.156
3005
X-RAY DIFFRACTION
99
Refinement TLS params.
Method: refined / Refine-ID: X-RAY DIFFRACTION
ID
L11 (°2)
L12 (°2)
L13 (°2)
L22 (°2)
L23 (°2)
L33 (°2)
S11 (Å °)
S12 (Å °)
S13 (Å °)
S21 (Å °)
S22 (Å °)
S23 (Å °)
S31 (Å °)
S32 (Å °)
S33 (Å °)
T11 (Å2)
T12 (Å2)
T13 (Å2)
T22 (Å2)
T23 (Å2)
T33 (Å2)
Origin x (Å)
Origin y (Å)
Origin z (Å)
1
0.868
-0.1446
-0.3387
1.0143
0.2149
6.1239
-0.0106
0.1732
0.0098
-0.0124
-0.1022
0.0387
0.0834
-0.3287
0.0569
0.3967
0.0153
0.0195
0.2678
-0.0129
0.2675
11.3534
4.6793
22.6699
2
0.8758
-0.0668
-0.0892
0.9438
0.3094
3.6311
-0.0201
0.0028
0.0113
-0.1461
-0.081
-0.007
0.1983
0.0994
0.0673
0.3095
0.0311
0.0253
0.2333
0.0124
0.261
12.4189
4.9199
60.1713
Refinement TLS group
ID
Refine-ID
Refine TLS-ID
Selection details
1
X-RAY DIFFRACTION
1
(CHAINAANDRESID299:716)
2
X-RAY DIFFRACTION
2
(CHAINBANDRESID299:718)
+
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