Structure of bovine endothelial nitric oxide synthase heme domain in complex with (9aS)-2-amino-9a-methyl-8,9,9a,10-tetrahydrobenzo[g]pteridine-4,6(3H,7H)-dione
要素
NITRIC OXIDE SYNTHASE, ENDOTHELIAL
キーワード
OXIDOREDUCTASE / COFACTOR ANALOG COMPLEX
機能・相同性
機能・相同性情報
cellular response to laminar fluid shear stress / positive regulation of guanylate cyclase activity / negative regulation of leukocyte cell-cell adhesion / nitric-oxide synthase (NADPH) / nitric oxide mediated signal transduction / nitric-oxide synthase activity / arginine catabolic process / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / nitric oxide biosynthetic process / negative regulation of blood pressure ...cellular response to laminar fluid shear stress / positive regulation of guanylate cyclase activity / negative regulation of leukocyte cell-cell adhesion / nitric-oxide synthase (NADPH) / nitric oxide mediated signal transduction / nitric-oxide synthase activity / arginine catabolic process / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / nitric oxide biosynthetic process / negative regulation of blood pressure / response to hormone / mitochondrion organization / caveola / blood coagulation / FMN binding / NADP binding / flavin adenine dinucleotide binding / response to lipopolysaccharide / cytoskeleton / calmodulin binding / heme binding / Golgi apparatus / nucleus / metal ion binding / plasma membrane / cytosol 類似検索 - 分子機能
NITRICOXIDESYNTHASE, ENDOTHELIAL / CONSTITUTIVE NOS / CNOS / EC-NOS / ENDOTHELIAL NOS / ENOS / NOS TYPE III / NOSIII / ENDOTHELIAL ...CONSTITUTIVE NOS / CNOS / EC-NOS / ENDOTHELIAL NOS / ENOS / NOS TYPE III / NOSIII / ENDOTHELIAL NITRIC OXIDE SYNTHASE
モノクロメーター: GRAPHITE / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.12709 Å / 相対比: 1
反射
解像度: 2.48→50 Å / Num. obs: 35505 / % possible obs: 99.5 % / Observed criterion σ(I): -3 / 冗長度: 3.9 % / Rmerge(I) obs: 0.08 / Net I/σ(I): 22.85
反射 シェル
解像度: 2.48→2.57 Å / 冗長度: 3.8 % / Rmerge(I) obs: 0.69 / Mean I/σ(I) obs: 2.01 / % possible all: 99.6
-
解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.6.0117
精密化
HKL-2000
データ削減
SCALEPACK
データスケーリング
REFMAC
位相決定
精密化
構造決定の手法: OTHER 開始モデル: NONE 解像度: 2.48→87.9 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.938 / SU B: 23.395 / SU ML: 0.245 / 交差検証法: THROUGHOUT / ESU R: 0.452 / ESU R Free: 0.272 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED. RESIDUES 110 TO 120 IN CHAIN A AND 112-120 IN CHAIN B ARE DISORDERED.
Rfactor
反射数
%反射
Selection details
Rfree
0.24223
1762
5 %
RANDOM
Rwork
0.18367
-
-
-
obs
0.18654
33702
99.43 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK