- EMDB-4734: Structure of the core Shigella flexneri type III secretion system... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-4734
タイトル
Structure of the core Shigella flexneri type III secretion system export gate complex SctRST (Spa24/Spa9/Spa29).
マップデータ
試料
複合体: Shigella flexneri type III secretion export gate (Spa24Spa9Spa29)
タンパク質・ペプチド: Surface presentation of antigens protein SpaP
タンパク質・ペプチド: Surface presentation of antigens protein SpaR
タンパク質・ペプチド: Surface presentation of antigens protein SpaQ
キーワード
Type III secretion / T3SS / Flagella / Cryo-EM / Membrane protein / Protein transport
機能・相同性
機能・相同性情報
protein secretion / protein targeting / plasma membrane 類似検索 - 分子機能
Type III secretion protein SpaR/YscT / Type III secretion protein HrpO / Yop virulence translocation protein R / Type III secretion system inner membrane R protein / Bacterial export protein family 3 / Bacterial export proteins, family 1 / Bacterial export proteins, family 3 / Flagella transport protein fliP family signature 1. / Type III secretion system inner membrane P protein / FliP family / Flagella transport protein fliP family signature 2. 類似検索 - ドメイン・相同性
Surface presentation of antigens protein SpaP / Surface presentation of antigens protein SpaQ / Surface presentation of antigens protein SpaR 類似検索 - 構成要素
ジャーナル: Nat Struct Mol Biol / 年: 2018 タイトル: Structure of the core of the type III secretion system export apparatus. 著者: Lucas Kuhlen / Patrizia Abrusci / Steven Johnson / Joseph Gault / Justin Deme / Joseph Caesar / Tobias Dietsche / Mehari Tesfazgi Mebrhatu / Tariq Ganief / Boris Macek / Samuel Wagner / Carol ...著者: Lucas Kuhlen / Patrizia Abrusci / Steven Johnson / Joseph Gault / Justin Deme / Joseph Caesar / Tobias Dietsche / Mehari Tesfazgi Mebrhatu / Tariq Ganief / Boris Macek / Samuel Wagner / Carol V Robinson / Susan M Lea / 要旨: Export of proteins through type III secretion systems is critical for motility and virulence of many major bacterial pathogens. Three putative integral membrane proteins (FliP, FliQ, FliR) are ...Export of proteins through type III secretion systems is critical for motility and virulence of many major bacterial pathogens. Three putative integral membrane proteins (FliP, FliQ, FliR) are suggested to form the core of an export gate in the inner membrane, but their structure, assembly and location within the final nanomachine remain unclear. Here, we present the cryoelectron microscopy structure of the Salmonella Typhimurium FliP-FliQ-FliR complex at 4.2 Å. None of the subunits adopt canonical integral membrane protein topologies, and common helix-turn-helix structural elements allow them to form a helical assembly with 5:4:1 stoichiometry. Fitting of the structure into reconstructions of intact secretion systems, combined with cross-linking, localize the export gate as a core component of the periplasmic portion of the machinery. This study thereby identifies the export gate as a key element of the secretion channel and implies that it primes the helical architecture of the components assembling downstream.