H2020 Marie Curie Actions of the European Commission
765703
European Union
Independent Research Fund Denmark - Technology and Production Sciences
9040-00425B
デンマーク
Natural Sciences and Engineering Research Council (NSERC, Canada)
532417
カナダ
The Carlsberg Foundation
CF20-0635
デンマーク
European Research Council (ERC)
683305
European Union
Novo Nordisk Foundation
0060694
デンマーク
引用
ジャーナル: Sci Adv / 年: 2024 タイトル: An RNA origami robot that traps and releases a fluorescent aptamer. 著者: Néstor Sampedro Vallina / Ewan K S McRae / Cody Geary / Ebbe S Andersen / 要旨: RNA nanotechnology aims to use RNA as a programmable material to create self-assembling nanodevices for application in medicine and synthetic biology. The main challenge is to develop advanced RNA ...RNA nanotechnology aims to use RNA as a programmable material to create self-assembling nanodevices for application in medicine and synthetic biology. The main challenge is to develop advanced RNA robotic devices that both sense, compute, and actuate to obtain enhanced control over molecular processes. Here, we use the RNA origami method to prototype an RNA robotic device, named the "Traptamer," that mechanically traps the fluorescent aptamer, iSpinach. The Traptamer is shown to sense two RNA key strands, acts as a Boolean AND gate, and reversibly controls the fluorescence of the iSpinach aptamer. Cryo-electron microscopy of the closed Traptamer structure at 5.45-angstrom resolution reveals the mechanical mode of distortion of the iSpinach motif. Our study suggests a general approach to distorting RNA motifs and a path forward to build sophisticated RNA machines that through sensing, computing, and actuation modules can be used to precisely control RNA functionalities in cellular systems.
名称: 14-14 Traptamer / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all 詳細: An RNA origami 3 helix tile with an inactive broccoli aptamer in the central helix.