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- PDB-3eq9: Prolyl oligopeptidase complexed with R-Pro-(decarboxy-Pro)-Type i... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3eq9 | ||||||
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Title | Prolyl oligopeptidase complexed with R-Pro-(decarboxy-Pro)-Type inhibitors | ||||||
![]() | Prolyl endopeptidase | ||||||
![]() | Hydrolase/Hydrolase inhibitor / protease-inhibitor complex / Hydrolase / Protease / Serine protease / Hydrolase-Hydrolase inhibitor complex | ||||||
Function / homology | ![]() prolyl oligopeptidase / oligopeptidase activity / serine-type endopeptidase activity / proteolysis / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Kanai, K. / Aranyi, P. / Bocskei, Z. / Ferenczy, G. / Harmat, V. / Simon, K. / Naray-Szabo, G. / Hermecz, I. | ||||||
![]() | ![]() Title: Prolyl oligopeptidase inhibition by N-acyl-pro-pyrrolidine-type molecules Authors: Kanai, K. / Aranyi, P. / Bocskei, Z. / Ferenczy, G. / Harmat, V. / Simon, K. / Batori, S. / Naray-Szabo, G. / Hermecz, I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 143 KB | Display | ![]() |
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PDB format | ![]() | 112.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3eq7C ![]() 3eq8C ![]() 1qfsS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 80864.344 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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#2: Chemical | ChemComp-X97 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.59 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 17% MPEG 5000, 15% glycerol, 20mM calcium acetate, 0.1M TRIS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Jun 11, 1998 / Details: capillary collimator |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.47→34.5 Å / Num. all: 28405 / Num. obs: 28405 / % possible obs: 94.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 3.4 % / Biso Wilson estimate: 31.94 Å2 / Rmerge(I) obs: 0.16 / Net I/σ(I): 4 |
Reflection shell | Resolution: 2.47→2.6 Å / Rmerge(I) obs: 0.441 / Num. unique all: 3140 / % possible all: 74.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1QFS Resolution: 2.47→34.5 Å Isotropic thermal model: Grouped isotropic B-factors, 2 B-values/residue Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 27.18 Å2 | ||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.2436 Å / Luzzati sigma a obs: 0.246 Å | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.47→34.5 Å
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Refine LS restraints |
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