- EMDB-37416: Cryo-EM structure of Snf7 N-terminal domain in outer coils of spi... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-37416
タイトル
Cryo-EM structure of Snf7 N-terminal domain in outer coils of spiral polymers
マップデータ
試料
複合体: Snf7 spiral polymers
タンパク質・ペプチド: SNF7 isoform 1
キーワード
membrane fission / spiral polymers / N-terminal domain / "open" status / PROTEIN TRANSPORT
機能・相同性
ESCRT III complex / Snf7 family / Snf7 / late endosome to vacuole transport via multivesicular body sorting pathway / vesicle budding from membrane / multivesicular body / cytoplasmic side of plasma membrane / Vacuolar-sorting protein SNF7
ジャーナル: Proc Natl Acad Sci U S A / 年: 2024 タイトル: Three-dimensional architecture of ESCRT-III flat spirals on the membrane. 著者: Mingdong Liu / Yunhui Liu / Tiefeng Song / Liuyan Yang / Lei Qi / Yu-Zhong Zhang / Yong Wang / Qing-Tao Shen / 要旨: The endosomal sorting complexes required for transport (ESCRTs) are responsible for membrane remodeling in many cellular processes, such as multivesicular body biogenesis, viral budding, and ...The endosomal sorting complexes required for transport (ESCRTs) are responsible for membrane remodeling in many cellular processes, such as multivesicular body biogenesis, viral budding, and cytokinetic abscission. ESCRT-III, the most abundant ESCRT subunit, assembles into flat spirals as the primed state, essential to initiate membrane invagination. However, the three-dimensional architecture of ESCRT-III flat spirals remained vague for decades due to highly curved filaments with a small diameter and a single preferred orientation on the membrane. Here, we unveiled that yeast Snf7, a component of ESCRT-III, forms flat spirals on the lipid monolayers using cryogenic electron microscopy. We developed a geometry-constrained Euler angle-assigned reconstruction strategy and obtained moderate-resolution structures of Snf7 flat spirals with varying curvatures. Our analyses showed that Snf7 subunits recline on the membrane with N-terminal motifs α0 as anchors, adopt an open state with fused α2/3 helices, and bend α2/3 gradually from the outer to inner parts of flat spirals. In all, we provide the orientation and conformations of ESCRT-III flat spirals on the membrane and unveil the underlying assembly mechanism, which will serve as the initial step in understanding how ESCRTs drive membrane abscission.