EMDB-35113: The cryo-EM structure of human C complex

Basic information

Entry

Database: EMDB / ID: EMD-35113

• Title: The cryo-EM structure of human C complex

Sample

• Complex: The human Bact-III complex
  • Protein or peptide: x 27 types
  • RNA: x 3 types
• Protein or peptide: x 16 types
• RNA: x 2 types
• Ligand: x 4 types

• Keywords: spliceosome / RNA splicing / PRP16 / branching / SPLICING

Function / homology

Function:

striated muscle dense body / exon-exon junction subcomplex mago-y14 / negative regulation of selenocysteine incorporation / regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / post-spliceosomal complex / cellular response to selenite ion / selenocysteine insertion sequence binding / exon-exon junction complex / regulation of translation at postsynapse, modulating synaptic transmission / regulation of retinoic acid receptor signaling pathway / post-mRNA release spliceosomal complex / U2 snRNP binding / U7 snRNA binding / negative regulation of excitatory postsynaptic potential / histone pre-mRNA DCP binding / intracellular mRNA localization / 3'-5' RNA helicase activity / U7 snRNP / generation of catalytic spliceosome for first transesterification step / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / regulation of vitamin D receptor signaling pathway / histone pre-mRNA 3'end processing complex / cis assembly of pre-catalytic spliceosome / regulation of mRNA processing / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / Deadenylation of mRNA / protein methylation / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / U12-type spliceosomal complex / embryonic brain development / methylosome / nuclear retinoic acid receptor binding / 7-methylguanosine cap hypermethylation / positive regulation of androgen receptor activity / M-decay: degradation of maternal mRNAs by maternally stored factors / Prp19 complex / poly(A) binding / U1 snRNP binding / pICln-Sm protein complex / embryonic cranial skeleton morphogenesis / mRNA 3'-end processing / sno(s)RNA-containing ribonucleoprotein complex / snRNP binding / U2-type catalytic step 1 spliceosome / RNA splicing, via transesterification reactions / small nuclear ribonucleoprotein complex / pre-mRNA binding / regulation of mRNA splicing, via spliceosome / C2H2 zinc finger domain binding / SMN-Sm protein complex / telomerase RNA binding / spliceosomal tri-snRNP complex / telomerase holoenzyme complex / U2-type spliceosomal complex / U2-type precatalytic spliceosome / positive regulation by host of viral transcription / mRNA cis splicing, via spliceosome / P granule / positive regulation of vitamin D receptor signaling pathway / commitment complex / U2-type prespliceosome assembly / nuclear vitamin D receptor binding / Transport of Mature mRNA derived from an Intron-Containing Transcript / U2-type catalytic step 2 spliceosome / Notch binding / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / U4 snRNP / positive regulation of mRNA splicing, via spliceosome / RUNX3 regulates NOTCH signaling / U2 snRNP / NOTCH4 Intracellular Domain Regulates Transcription / RNA Polymerase II Transcription Termination / U1 snRNP / exploration behavior / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / NOTCH3 Intracellular Domain Regulates Transcription / ubiquitin-ubiquitin ligase activity / positive regulation of neurogenesis / WD40-repeat domain binding / U2-type prespliceosome / lipid biosynthetic process / nuclear androgen receptor binding / regulation of alternative mRNA splicing, via spliceosome / precatalytic spliceosome / K63-linked polyubiquitin modification-dependent protein binding / muscle organ development / cyclosporin A binding / Notch-HLH transcription pathway / generation of catalytic spliceosome for second transesterification step / positive regulation of transforming growth factor beta receptor signaling pathway / Formation of paraxial mesoderm / mRNA Splicing - Minor Pathway / spliceosomal complex assembly / SMAD binding / mitotic G2 DNA damage checkpoint signaling / associative learning / negative regulation of DNA damage response, signal transduction by p53 class mediator / protein K63-linked ubiquitination / mRNA 3'-splice site recognition

Domain/homology:

Corepressor interacting with RBPJ 1 / : / Protein FRG1 / FRG1-like domain / CBF1-interacting co-repressor CIR, N-terminal domain / Pre-mRNA splicing factor / : / N-terminal domain of CBF1 interacting co-repressor CIR / Pre-mRNA splicing factor / N-terminal domain of CBF1 interacting co-repressor CIR / CASC3/Barentsz eIF4AIII binding / CASC3/Barentsz eIF4AIII binding / Btz domain / Saf4/Yju2 protein / Splicing factor Yju2 / Saf4/Yju2 protein / Mago nashi protein / RNA-binding motif protein 8 / RBM8, RNA recognition motif / Mago nashi superfamily / Mago nashi protein / Actin-crosslinking / Pre-mRNA-splicing factor Isy1 / Pre-mRNA-splicing factor Isy1 superfamily / Isy1-like splicing family / : / : / : / Intron-binding protein aquarius, beta-barrel / Intron-binding protein aquarius insert domain / Pre-mRNA-splicing factor SPF27 / Breast carcinoma amplified sequence 2 (BCAS2) / Peptidyl-prolyl cis-trans isomerase E / Peptidyl-prolyl cis-trans isomerase E, RNA recognition motif / CWF11 family / mRNA splicing factor SYF2 / SYF2 splicing factor / Intron-binding protein aquarius, N-terminal / Intron-binding protein aquarius N-terminal / Helix hairpin bin domain superfamily / : / : / mRNA splicing factor Cwf21 domain / cwf21 domain / Pre-mRNA-processing factor 17 / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 2 / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 1 / HAT (Half-A-TPR) repeat / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / Prp19/Pso4-like / U-box domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / BUD31/G10-related, conserved site / G10 protein signature 1. / G10 protein signature 2. / : / STL11, N-terminal / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / DNA2/NAM7 helicase, helicase domain / Cwf15/Cwc15 cell cycle control protein / AAA domain / WD repeat Prp46/PLRG1-like / Pre-mRNA-splicing factor Cwc2/Slt11 / G10 protein / : / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / : / : / pre-mRNA splicing factor component / : / Myb-like DNA-binding domain / Initiation factor eIF-4 gamma, MA3 / MA3 domain / MI domain profile. / Domain in DAP-5, eIF4G, MA-3 and other proteins. / MIF4G domain / Small ribonucleoprotein associated, SmB/SmN / DNA2/NAM7-like helicase / Spt5 C-terminal domain / Spt5 C-terminal nonapeptide repeat binding Spt4 / Small nuclear ribonucleoprotein D1 / Middle domain of eukaryotic initiation factor 4G (eIF4G) / MIF4G-like, type 3 / U-box domain profile. / Modified RING finger domain / Zinc finger, CCCH-type superfamily / U-box domain / U2A'/phosphoprotein 32 family A, C-terminal / occurring C-terminal to leucine-rich repeats / Brr2, N-terminal helicase PWI domain / : / N-terminal helicase PWI domain / Pre-mRNA-splicing helicase BRR2 plug domain / Sec63 Brl domain / zinc finger

Component:

Protein CASC3 / Pleiotropic regulator 1 / RNA helicase aquarius / Pre-mRNA-processing factor 17 / U5 small nuclear ribonucleoprotein 200 kDa helicase / Pre-mRNA-splicing factor SPF27 / Pre-mRNA-splicing factor SYF2 / U2 small nuclear ribonucleoprotein B'' / U2 small nuclear ribonucleoprotein A' / Small nuclear ribonucleoprotein-associated proteins B and B' / Eukaryotic initiation factor 4A-III / Protein BUD31 homolog / Protein mago nashi homolog / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein Sm D1 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / SNW domain-containing protein 1 / Protein FRG1 / 116 kDa U5 small nuclear ribonucleoprotein component / Pre-mRNA-processing-splicing factor 8 / Corepressor interacting with RBPJ 1 / Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 / Peptidylprolyl isomerase domain and WD repeat-containing protein 1 / U5 small nuclear ribonucleoprotein 40 kDa protein / Cell division cycle 5-like protein / Splicing factor YJU2 / Crooked neck-like protein 1 / Pre-mRNA-splicing factor CWC22 homolog / Pre-mRNA-splicing factor SYF1 / Pre-mRNA-splicing factor RBM22 / WD repeat-containing protein 70 / Pre-mRNA-splicing factor CWC25 homolog / Spliceosome-associated protein CWC15 homolog / Pre-mRNA-splicing factor ISY1 homolog / Pre-mRNA-processing factor 19 / Peptidyl-prolyl cis-trans isomerase E / Serine/arginine repetitive matrix protein 2 / Peptidyl-prolyl cis-trans isomerase-like 1 / RNA-binding protein 8A

• Biological species: Homo sapiens (human) / unidentified adenovirus
• Method: single particle reconstruction / cryo EM / Resolution: 3.4 Å
• Authors: Zhan X / Lu Y / Shi Y

Funding support

China, 1 items

Organization	Grant number	Country
National Natural Science Foundation of China (NSFC)		China

• Citation: Journal: Nat Commun / Year: 2024; Title: Molecular basis for the activation of human spliceosome.; Authors: Xiechao Zhan / Yichen Lu / Yigong Shi / ; Abstract: The spliceosome executes pre-mRNA splicing through four sequential stages: assembly, activation, catalysis, and disassembly. Activation of the spliceosome, namely remodeling of the pre-catalytic spliceosome (B complex) into the activated spliceosome (B complex) and the catalytically activated spliceosome (B complex), involves major flux of protein components and structural rearrangements. Relying on a splicing inhibitor, we have captured six intermediate states between the B and B complexes: pre-B, B-I, B-II, B-III, B-IV, and post-B. Their cryo-EM structures, together with an improved structure of the catalytic step I spliceosome (C complex), reveal how the catalytic center matures around the internal stem loop of U6 snRNA, how the branch site approaches 5'-splice site, how the RNA helicase PRP2 rearranges to bind pre-mRNA, and how U2 snRNP undergoes remarkable movement to facilitate activation. We identify a previously unrecognized key role of PRP2 in spliceosome activation. Our study recapitulates a molecular choreography of the human spliceosome during its catalytic activation.

History

Deposition	Jan 11, 2023	-
Header (metadata) release	Jul 31, 2024	-
Map release	Jul 31, 2024	-
Update	Oct 16, 2024	-
Current status	Oct 16, 2024	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_35113.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.077 Å

Density

Contour Level	By AUTHOR: 0.25
Minimum - Maximum	-0.8991936 - 2.1206462
Average (Standard dev.)	0.004052774 (±0.0678683)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 516.96 Å α=β=γ: 90.0 °

Supplemental data

Half map: #2

• File: emd_35113_half_map_1.map

Half map: #1

• File: emd_35113_half_map_2.map

Sample components

Entire : The human Bact-III complex

• Entire: Name: The human Bact-III complex

Components

• Complex: The human Bact-III complex
  • Protein or peptide: Pre-mRNA-processing-splicing factor 8
  • RNA: U5 snRNA
  • Protein or peptide: 116 kDa U5 small nuclear ribonucleoprotein component
  • Protein or peptide: U5 small nuclear ribonucleoprotein 200 kDa helicase
  • Protein or peptide: U5 small nuclear ribonucleoprotein 40 kDa protein
  • RNA: U6 snRNA
  • RNA: U2 snRNA
  • Protein or peptide: Pre-mRNA-splicing factor SYF1
  • Protein or peptide: Crooked neck-like protein 1
  • Protein or peptide: Pre-mRNA-splicing factor SPF27
  • Protein or peptide: Cell division cycle 5-like protein
  • Protein or peptide: Pre-mRNA-splicing factor SYF2
  • Protein or peptide: Protein BUD31 homolog
  • Protein or peptide: Pre-mRNA-splicing factor RBM22
  • Protein or peptide: Spliceosome-associated protein CWC15 homolog
  • Protein or peptide: SNW domain-containing protein 1
  • Protein or peptide: Peptidyl-prolyl cis-trans isomerase-like 1
  • Protein or peptide: Pleiotropic regulator 1
  • Protein or peptide: RNA helicase aquarius
  • Protein or peptide: Serine/arginine repetitive matrix protein 2
  • Protein or peptide: Pre-mRNA-splicing factor CWC22 homolog
  • Protein or peptide: Pre-mRNA-processing factor 17
  • Protein or peptide: Pre-mRNA-splicing factor CWC25 homolog
  • Protein or peptide: Coiled-coil domain-containing protein 94
  • Protein or peptide: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
  • Protein or peptide: Pre-mRNA-processing factor 19
  • Protein or peptide: Eukaryotic initiation factor 4A-III
  • Protein or peptide: Protein CASC3
  • Protein or peptide: RNA-binding protein 8A
  • Protein or peptide: Protein mago nashi homolog
• Protein or peptide: Small nuclear ribonucleoprotein Sm D3
• Protein or peptide: Small nuclear ribonucleoprotein-associated proteins B and B'
• Protein or peptide: Small nuclear ribonucleoprotein Sm D1
• Protein or peptide: Small nuclear ribonucleoprotein Sm D2
• Protein or peptide: Small nuclear ribonucleoprotein F
• Protein or peptide: Small nuclear ribonucleoprotein E
• Protein or peptide: Small nuclear ribonucleoprotein G
• Protein or peptide: U2 small nuclear ribonucleoprotein A'
• Protein or peptide: U2 small nuclear ribonucleoprotein B''
• Protein or peptide: Peptidyl-prolyl cis-trans isomerase E
• Protein or peptide: Pre-mRNA-splicing factor ISY1 homolog
• Protein or peptide: Peptidylprolyl isomerase domain and WD repeat-containing protein 1
• Protein or peptide: Corepressor interacting with RBPJ 1
• Protein or peptide: WD repeat-containing protein 70
• Protein or peptide: Protein FRG1
• Protein or peptide: UNK
• RNA: pre-mRNA
• RNA: pre-mRNA
• Ligand: INOSITOL HEXAKISPHOSPHATE
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION

Supramolecule #1: The human Bact-III complex

• Supramolecule: Name: The human Bact-III complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#6, #9-#29, #32, #31, #30
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: Pre-mRNA-processing-splicing factor 8

• Macromolecule: Name: Pre-mRNA-processing-splicing factor 8 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 273.97425 KDa

Sequence

String:

MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP FDDEEPPLDY ADNILDVEPL EAIQLELDPE EDAPVLDWFY DHQPLRDSRK YVNGSTYQRW QFTLPMMSTL YR LANQLLT DLVDDNYFYL FDLKAFFTSK ALNMAIPGGP KFEPLVRDIN LQDEDWNEFN DINKIIIRQP IRTEYKIAFP YLY NNLPHH VHLTWYHTPN VVFIKTEDPD LPAFYFDPLI NPISHRHSVK SQEPLPDDDE EFELPEFVEP FLKDTPLYTD NTAN GIALL WAPRPFNLRS GRTRRALDIP LVKNWYREHC PAGQPVKVRV SYQKLLKYYV LNALKHRPPK AQKKRYLFRS FKATK FFQS TKLDWVEVGL QVCRQGYNML NLLIHRKNLN YLHLDYNFNL KPVKTLTTKE RKKSRFGNAF HLCREVLRLT KLVVDS HVQ YRLGNVDAFQ LADGLQYIFA HVGQLTGMYR YKYKLMRQIR MCKDLKHLIY YRFNTGPVGK GPGCGFWAAG WRVWLFF MR GITPLLERWL GNLLARQFEG RHSKGVAKTV TKQRVESHFD LELRAAVMHD ILDMMPEGIK QNKARTILQH LSEAWRCW K ANIPWKVPGL PTPIENMILR YVKAKADWWT NTAHYNRERI RRGATVDKTV CKKNLGRLTR LYLKAEQERQ HNYLKDGPY ITAEEAVAVY TTTVHWLESR RFSPIPFPPL SYKHDTKLLI LALERLKEAY SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQR AFKEVGIEFM DLYSHLVPVY DVEPLEKITD AYLDQYLWYE ADKRRLFPPW IKPADTEPPP LLVYKWCQGI N NLQDVWET SEGECNVMLE SRFEKMYEKI DLTLLNRLLR LIVDHNIADY MTAKNNVVIN YKDMNHTNSY GIIRGLQFAS FI VQYYGLV MDLLVLGLHR ASEMAGPPQM PNDFLSFQDI ATEAAHPIRL FCRYIDRIHI FFRFTADEAR DLIQRYLTEH PDP NNENIV GYNNKKCWPR DARMRLMKHD VNLGRAVFWD IKNRLPRSVT TVQWENSFVS VYSKDNPNLL FNMCGFECRI LPKC RTSYE EFTHKDGVWN LQNEVTKERT AQCFLRVDDE SMQRFHNRVR QILMASGSTT FTKIVNKWNT ALIGLMTYFR EAVVN TQEL LDLLVKCENK IQTRIKIGLN SKMPSRFPPV VFYTPKELGG LGMLSMGHVL IPQSDLRWSK QTDVGITHFR SGMSHE EDQ LIPNLYRYIQ PWESEFIDSQ RVWAEYALKR QEAIAQNRRL TLEDLEDSWD RGIPRINTLF QKDRHTLAYD KGWRVRT DF KQYQVLKQNP FWWTHQRHDG KLWNLNNYRT DMIQALGGVE GILEHTLFKG TYFPTWEGLF WEKASGFEES MKWKKLTN A QRSGLNQIPN RRFTLWWSPT INRANVYVGF QVQLDLTGIF MHGKIPTLKI SLIQIFRAHL WQKIHESIVM DLCQVFDQE LDALEIETVQ KETIHPRKSY KMNSSCADIL LFASYKWNVS RPSLLADSKD VMDSTTTQKY WIDIQLRWGD YDSHDIERYA RAKFLDYTT DNMSIYPSPT GVLIAIDLAY NLHSAYGNWF PGSKPLIQQA MAKIMKANPA LYVLRERIRK GLQLYSSEPT E PYLSSQNY GELFSNQIIW FVDDTNVYRV TIHKTFEGNL TTKPINGAIF IFNPRTGQLF LKIIHTSVWA GQKRLGQLAK WK TAEEVAA LIRSLPVEEQ PKQIIVTRKG MLDPLEVHLL DFPNIVIKGS ELQLPFQACL KVEKFGDLIL KATEPQMVLF NLY DDWLKT ISSYTAFSRL ILILRALHVN NDRAKVILKP DKTTITEPHH IWPTLTDEEW IKVEVQLKDL ILADYGKKNN VNVA SLTQS EIRDIILGME ISAPSQQRQQ IAEIEKQTKE QSQLTATQTR TVNKHGDEII TSTTSNYETQ TFSSKTEWRV RAISA ANLH LRTNHIYVSS DDIKETGYTY ILPKNVLKKF ICISDLRAQI AGYLYGVSPP DNPQVKEIRC IVMVPQWGTH QTVHLP GQL PQHEYLKEME PLGWIHTQPN ESPQLSPQDV TTHAKIMADN PSWDGEKTII ITCSFTPGSC TLTAYKLTPS GYEWGRQ NT DKGNNPKGYL PSHYERVQML LSDRFLGFFM VPAQSSWNYN FMGVRHDPNM KYELQLANPK EFYHEVHRPS HFLNFALL Q EGEVYSADRE DLYA

UniProtKB: Pre-mRNA-processing-splicing factor 8

Macromolecule #3: 116 kDa U5 small nuclear ribonucleoprotein component

• Macromolecule: Name: 116 kDa U5 small nuclear ribonucleoprotein component / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 109.560625 KDa

Sequence

String:

MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEMDDDD DDDDVGDHDD DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPL TEPIIKPVKT KKFTLMEQTL PVTVYEMDFL ADLMDNSELI RNVTLCGHLH HGKTCFVDCL IEQTHPEIRK R YDQDLCYT DILFTEQERG VGIKSTPVTV VLPDTKGKSY LFNIMDTPGH VNFSDEVTAG LRISDGVVLF IDAAEGVMLN TE RLIKHAV QERLAVTVCI NKIDRLILEL KLPPTDAYYK LRHIVDEVNG LISMYSTDEN LILSPLLGNV CFSSSQYSIC FTL GSFAKI YADTFGDINY QEFAKRLWGD IYFNPKTRKF TKKAPTSSSQ RSFVEFILEP LYKILAQVVG DVDTSLPRTL DELG IHLTK EELKLNIRPL LRLVCKKFFG EFTGFVDMCV QHIPSPKVGA KPKIEHTYTG GVDSDLGEAM SDCDPDGPLM CHTTK MYST DDGVQFHAFG RVLSGTIHAG QPVKVLGENY TLEDEEDSQI CTVGRLWISV ARYHIEVNRV PAGNWVLIEG VDQPIV KTA TITEPRGNEE AQIFRPLKFN TTSVIKIAVE PVNPSELPKM LDGLRKVNKS YPSLTTKVEE SGEHVILGTG ELYLDCV MH DLRKMYSEID IKVADPVVTF CETVVETSSL KCFAETPNKK NKITMIAEPL EKGLAEDIEN EVVQITWNRK KLGEFFQT K YDWDLLAARS IWAFGPDATG PNILVDDTLP SEVDKALLGS VKDSIVQGFQ WGTREGPLCD ELIRNVKFKI LDAVVAQEP LHRGGGQIIP TARRVVYSAF LMATPRLMEP YYFVEVQAPA DCVSAVYTVL ARRRGHVTQD APIPGSPLYT IKAFIPAIDS FGFETDLRT HTQGQAFSLS VFHHWQIVPG DPLDKSIVIR PLEPQPAPHL AREFMIKTRR RKGLSEDVSI SKFFDDPMLL E LAKQDVVL NYPM

UniProtKB: 116 kDa U5 small nuclear ribonucleoprotein component

Macromolecule #4: U5 small nuclear ribonucleoprotein 200 kDa helicase

• Macromolecule: Name: U5 small nuclear ribonucleoprotein 200 kDa helicase / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 244.823422 KDa

Sequence

String:

MADVTARSLQ YEYKANSNLV LQADRSLIDR TRRDEPTGEV LSLVGKLEGT RMGDKAQRTK PQMQEERRAK RRKRDEDRHD INKMKGYTL LSEGIDEMVG IIYKPKTKET RETYEVLLSF IQAALGDQPR DILCGAADEV LAVLKNEKLR DKERRKEIDL L LGQTDDTR YHVLVNLGKK ITDYGGDKEI QNMDDNIDET YGVNVQFESD EEEGDEDVYG EVREEASDDD MEGDEAVVRC TL SANLVAS GELMSSKKKD LHPRDIDAFW LQRQLSRFYD DAIVSQKKAD EVLEILKTAS DDRECENQLV LLLGFNTFDF IKV LRQHRM MILYCTLLAS AQSEAEKERI MGKMEADPEL SKFLYQLHET EKEDLIREER SRRERVRQSR MDTDLETMDL DQGG EALAP RQVLDLEDLV FTQGSHFMAN KRCQLPDGSF RRQRKGYEEV HVPALKPKPF GSEEQLLPVE KLPKYAQAGF EGFKT LNRI QSKLYRAALE TDENLLLCAP TGAGKTNVAL MCMLREIGKH INMDGTINVD DFKIIYIAPM RSLVQEMVGS FGKRLA TYG ITVAELTGDH QLCKEEISAT QIIVCTPEKW DIITRKGGER TYTQLVRLII LDEIHLLHDD RGPVLEALVA RAIRNIE MT QEDVRLIGLS ATLPNYEDVA TFLRVDPAKG LFYFDNSFRP VPLEQTYVGI TEKKAIKRFQ IMNEIVYEKI MEHAGKNQ V LVFVHSRKET GKTARAIRDM CLEKDTLGLF LREGSASTEV LRTEAEQCKN LELKDLLPYG FAIHHAGMTR VDRTLVEDL FADKHIQVLV STATLAWGVN LPAHTVIIKG TQVYSPEKGR WTELGALDIL QMLGRAGRPQ YDTKGEGILI TSHGELQYYL SLLNQQLPI ESQMVSKLPD MLNAEIVLGN VQNAKDAVNW LGYAYLYIRM LRSPTLYGIS HDDLKGDPLL DQRRLDLVHT A ALMLDKNN LVKYDKKTGN FQVTELGRIA SHYYITNDTV QTYNQLLKPT LSEIELFRVF SLSSEFKNIT VREEEKLELQ KL LERVPIP VKESIEEPSA KINVLLQAFI SQLKLEGFAL MADMVYVTQS AGRLMRAIFE IVLNRGWAQL TDKTLNLCKM IDK RMWQSM CPLRQFRKLP EEVVKKIEKK NFPFERLYDL NHNEIGELIR MPKMGKTIHK YVHLFPKLEL SVHLQPITRS TLKV ELTIT PDFQWDEKVH GSSEAFWILV EDVDSEVILH HEYFLLKAKY AQDEHLITFF VPVFEPLPPQ YFIRVVSDRW LSCET QLPV SFRHLILPEK YPPPTELLDL QPLPVSALRN SAFESLYQDK FPFFNPIQTQ VFNTVYNSDD NVFVGAPTGS GKTICA EFA ILRMLLQSSE GRCVYITPME ALAEQVYMDW YEKFQDRLNK KVVLLTGETS TDLKLLGKGN IIISTPEKWD ILSRRWK QR KNVQNINLFV VDEVHLIGGE NGPVLEVICS RMRYISSQIE RPIRIVALSS SLSNAKDVAH WLGCSATSTF NFHPNVRP V PLELHIQGFN ISHTQTRLLS MAKPVYHAIT KHSPKKPVIV FVPSRKQTRL TAIDILTTCA ADIQRQRFLH CTEKDLIPY LEKLSDSTLK ETLLNGVGYL HEGLSPMERR LVEQLFSSGA IQVVVASRSL CWGMNVAAHL VIIMDTQYYN GKIHAYVDYP IYDVLQMVG HANRPLQDDE GRCVIMCQGS KKDFFKKFLY EPLPVESHLD HCMHDHFNAE IVTKTIENKQ DAVDYLTWTF L YRRMTQNP NYYNLQGISH RHLSDHLSEL VEQTLSDLEQ SKCISIEDEM DVAPLNLGMI AAYYYINYTT IELFSMSLNA KT KVRGLIE IISNAAEYEN IPIRHHEDNL LRQLAQKVPH KLNNPKFNDP HVKTNLLLQA HLSRMQLSAE LQSDTEEILS KAI RLIQAC VDVLSSNGWL SPALAAMELA QMVTQAMWSK DSYLKQLPHF TSEHIKRCTD KGVESVFDIM EMEDEERNAL LQLT DSQIA DVARFCNRYP NIELSYEVVD KDSIRSGGPV VVLVQLEREE EVTGPVIAPL FPQKREEGWW VVIGDAKSNS LISIK RLTL QQKAKVKLDF VAPATGAHNY TLYFMSDAYM GCDQEYKFSV DVKEAETDSD SD

UniProtKB: U5 small nuclear ribonucleoprotein 200 kDa helicase

Macromolecule #5: U5 small nuclear ribonucleoprotein 40 kDa protein

• Macromolecule: Name: U5 small nuclear ribonucleoprotein 40 kDa protein / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 39.359492 KDa

Sequence

String:

MIEQQKRKGP ELPLVPVKRQ RHELLLGAGS GPGAGQQQAT PGALLQAGPP RCSSLQAPIM LLSGHEGEVY CCKFHPNGST LASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP A RRGPQLVC TGSDDGTVKL WDIRKKAAIQ TFQNTYQVLA VTFNDTSDQI ISGGIDNDIK VWDLRQNKLT YTMRGHADSV TG LSLSSEG SYLLSNAMDN TVRVWDVRPF APKERCVKIF QGNVHNFEKN LLRCSWSPDG SKIAAGSADR FVYVWDTTSR RIL YKLPGH AGSINEVAFH PDEPIIISAS SDKRLYMGEI Q

UniProtKB: U5 small nuclear ribonucleoprotein 40 kDa protein

Macromolecule #10: Pre-mRNA-splicing factor SYF1

• Macromolecule: Name: Pre-mRNA-splicing factor SYF1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 100.148711 KDa

Sequence

String:

MVVMARLSRP ERPDLVFEEE DLPYEEEIMR NQFSVKCWLR YIEFKQGAPK PRLNQLYERA LKLLPCSYKL WYRYLKARRA QVKHRCVTD PAYEDVNNCH ERAFVFMHKM PRLWLDYCQF LMDQGRVTHT RRTFDRALRA LPITQHSRIW PLYLRFLRSH P LPETAVRG YRRFLKLSPE SAEEYIEYLK SSDRLDEAAQ RLATVVNDER FVSKAGKSNY QLWHELCDLI SQNPDKVQSL NV DAIIRGG LTRFTDQLGK LWCSLADYYI RSGHFEKARD VYEEAIRTVM TVRDFTQVFD SYAQFEESMI AAKMETASEL GRE EEDDVD LELRLARFEQ LISRRPLLLN SVLLRQNPHH VHEWHKRVAL HQGRPREIIN TYTEAVQTVD PFKATGKPHT LWVA FAKFY EDNGQLDDAR VILEKATKVN FKQVDDLASV WCQCGELELR HENYDEALRL LRKATALPAR RAEYFDGSEP VQNRV YKSL KVWSMLADLE ESLGTFQSTK AVYDRILDLR IATPQIVINY AMFLEEHKYF EESFKAYERG ISLFKWPNVS DIWSTY LTK FIARYGGRKL ERARDLFEQA LDGCPPKYAK TLYLLYAQLE EEWGLARHAM AVYERATRAV EPAQQYDMFN IYIKRAA EI YGVTHTRGIY QKAIEVLSDE HAREMCLRFA DMECKLGEID RARAIYSFCS QICDPRTTGA FWQTWKDFEV RHGNEDTI K EMLRIRRSVQ ATYNTQVNFM ASQMLKVSGS ATGTVSDLAP GQSGMDDMKL LEQRAEQLAA EAERDQPLRA QSKILFVRS DASREELAEL AQQVNPEEIQ LGEDEDEDEM DLEPNEVRLE QQSVPAAVFG SLKED

UniProtKB: Pre-mRNA-splicing factor SYF1

Macromolecule #11: Crooked neck-like protein 1

• Macromolecule: Name: Crooked neck-like protein 1 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 100.610008 KDa

Sequence

String:

MTATVENLTF QKDTLGNAVD KNTSRLELRS YSLAGRHGST EPLVLAWSSQ FRRLTWGCAL DALHRSPCVA ASQHGVTHLI RSSRTPHST RCRKEDAQPG HHGNGAASVT AQARGQRSVL QVPLPVPRSC LFSESFVVSV SSQSRFLASV PGTGVQRSTA A DMAASTAA GKQRIPKVAK VKNKAPAEVQ ITAEQLLREA KERELELLPP PPQQKITDEE ELNDYKLRKR KTFEDNIRKN RT VISNWIK YAQWEESLKE IQRARSIYER ALDVDYRNIT LWLKYAEMEM KNRQVNHARN IWDRAITTLP RVNQFWYKYT YME EMLGNV AGARQVFERW MEWQPEEQAW HSYINFELRY KEVDRARTIY ERFVLVHPDV KNWIKYARFE EKHAYFAHAR KVYE RAVEF FGDEHMDEHL YVAFAKFEEN QKEFERVRVI YKYALDRISK QDAQELFKNY TIFEKKFGDR RGIEDIIVSK RRFQY EEEV KANPHNYDAW FDYLRLVESD AEAEAVREVY ERAIANVPPI QEKRHWKRYI YLWINYALYE ELEAKDPERT RQVYQA SLE LIPHKKFTFA KMWILYAQFE IRQKNLSLAR RALGTSIGKC PKNKLFKVYI ELELQLREFD RCRKLYEKFL EFGPENC TS WIKFAELETI LGDIDRARAI YELAISQPRL DMPEVLWKSY IDFEIEQEET ERTRNLYRRL LQRTQHVKVW ISFAQFEL S SGKEGSLTKC RQIYEEANKT MRNCEEKEER LMLLESWRSF EEEFGTASDK ERVDKLMPEK VKKRRKVQTD DGSDAGWEE YFDYIFPEDA ANQPNLKLLA MAKLWKKQQQ EKEDAEHHPD EDVDESES

UniProtKB: Crooked neck-like protein 1

Macromolecule #12: Pre-mRNA-splicing factor SPF27

• Macromolecule: Name: Pre-mRNA-splicing factor SPF27 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.16342 KDa

Sequence

String:

MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL SYLTAPDYSA FETDIMRNEF ERLAARQPIE LLSMKRYEL PAPSSGQKND ITAWQECVNN SMAQLEHQAV RIENLELMSQ HGCNAWKVYN ENLVHMIEHA QKELQKLRKH I QDLNWQRK NMQLTAGSKL REMESNWVSL VSKNYEIERT IVQLENEIYQ IKQQHGEANK ENIRQDF

UniProtKB: Pre-mRNA-splicing factor SPF27

Macromolecule #13: Cell division cycle 5-like protein

• Macromolecule: Name: Cell division cycle 5-like protein / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 92.406883 KDa

Sequence

String:

MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPII GRTAAQCLEH YEFLLDKAAQ RDNEEETTDD PRKLKPGEID PNPETKPARP DPIDMDEDEL EMLSEARARL A NTQGKKAK RKAREKQLEE ARRLAALQKR RELRAAGIEI QKKRKRKRGV DYNAEIPFEK KPALGFYDTS EENYQALDAD FR KLRQQDL DGELRSEKEG RDRKKDKQHL KRKKESDLPS AILQTSGVSE FTKKRSKLVL PAPQISDAEL QEVVKVGQAS EIA RQTAEE SGITNSASST LLSEYNVTNN SVALRTPRTP ASQDRILQEA QNLMALTNVD TPLKGGLNTP LHESDFSGVT PQRQ VVQTP NTVLSTPFRT PSNGAEGLTP RSGTTPKPVI NSTPGRTPLR DKLNINPEDG MADYSDPSYV KQMERESREH LRLGL LGLP APKNDFEIVL PENAEKELEE REIDDTYIED AADVDARKQA IRDAERVKEM KRMHKAVQKD LPRPSEVNET ILRPLN VEP PLTDLQKSEE LIKKEMITML HYDLLHHPYE PSGNKKGKTV GFGTNNSEHI TYLEHNPYEK FSKEELKKAQ DVLVQEM EV VKQGMSHGEL SSEAYNQVWE ECYSQVLYLP GQSRYTRANL ASKKDRIESL EKRLEINRGH MTTEAKRAAK MEKKMKIL L GGYQSRAMGL MKQLNDLWDQ IEQAHLELRT FEELKKHEDS AIPRRLECLK EDVQRQQERE KELQHRYADL LLEKETLKS KF

UniProtKB: Cell division cycle 5-like protein

Macromolecule #14: Pre-mRNA-splicing factor SYF2

• Macromolecule: Name: Pre-mRNA-splicing factor SYF2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.780518 KDa

Sequence

String:

MAAIAASEVL VDSAEEGSLA AAAELAAQKR EQRLRKFREL HLMRNEARKL NHQEVVEEDK RLKLPANWEA KKARLEWELK EEEKKKECA ARGEDYEKVK LLEISAEDAE RWERKKKRKN PDLGFSDYAA AQLRQYHRLT KQIKPDMETY ERLREKHGEE F FPTSNSLL HGTHVPSTEE IDRMVIDLEK QIEKRDKYSR RRPYNDDADI DYINERNAKF NKKAERFYGK YTAEIKQNLE RG TAV

UniProtKB: Pre-mRNA-splicing factor SYF2

Macromolecule #15: Protein BUD31 homolog

• Macromolecule: Name: Protein BUD31 homolog / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.03285 KDa

Sequence

String:

MPKVKRSRKA PPDGWELIEP TLDELDQKMR EAETEPHEGK RKVESLWPIF RIHHQKTRYI FDLFYKRKAI SRELYEYCIK EGYADKNLI AKWKKQGYEN LCCLRCIQTR DTNFGTNCIC RVPKSKLEVG RIIECTHCGC RGCSG

UniProtKB: Protein BUD31 homolog

Macromolecule #16: Pre-mRNA-splicing factor RBM22

• Macromolecule: Name: Pre-mRNA-splicing factor RBM22 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 46.959555 KDa

Sequence

String:

MATSLGSNTY NRQNWEDADF PILCQTCLGE NPYIRMTKEK YGKECKICAR PFTVFRWCPG VRMRFKKTEV CQTCSKLKNV CQTCLLDLE YGLPIQVRDA GLSFKDDMPK SDVNKEYYTQ NMEREISNSD GTRPVGMLGK ATSTSDMLLK LARTTPYYKR N RPHICSFW VKGECKRGEE CPYRHEKPTD PDDPLADQNI KDRYYGINDP VADKLLKRAS TMPRLDPPED KTITTLYVGG LG DTITETD LRNHFYQFGE IRTITVVQRQ QCAFIQFATR QAAEVAAEKS FNKLIVNGRR LNVKWGRSQA ARGKEKEKDG TTD SGIKLE PVPGLPGALP PPPAAEEEAS ANYFNLPPSG PPAVVNIALP PPPGIAPPPP PGFGPHMFHP MGPPPPFMRA PGPI HYPSQ DPQRMGAHAG KHSSP

UniProtKB: Pre-mRNA-splicing factor RBM22

Macromolecule #17: Spliceosome-associated protein CWC15 homolog

• Macromolecule: Name: Spliceosome-associated protein CWC15 homolog / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.674447 KDa

Sequence

String:

MTTAARPTFE PARGGRGKGE GDLSQLSKQY SSRDLPSHTK IKYRQTTQDA PEEVRNRDFR RELEERERAA AREKNRDRPT REHTTSSSV SKKPRLDQIP AANLDADDPL TDEEDEDFEE ESDDDDTAAL LAELEKIKKE RAEEQARKEQ EQKAEEERIR M ENILSGNP LLNLTGPSQP QANFKVKRRW DDDVVFKNCA KGVDDQKKDK RFVNDTLRSE FHKKFMEKYI K

UniProtKB: Spliceosome-associated protein CWC15 homolog

Macromolecule #18: SNW domain-containing protein 1

• Macromolecule: Name: SNW domain-containing protein 1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 61.770648 KDa

Sequence

String:

MALTSFLPAP TQLSQDQLEA EEKARSQRSR QTSLVSSRRE PPPYGYRKGW IPRLLEDFGD GGAFPEIHVA QYPLDMGRKK KMSNALAIQ VDSEGKIKYD AIARQGQSKD KVIYSKYTDL VPKEVMNADD PDLQRPDEEA IKEITEKTRV ALEKSVSQKV A AAMPVRAA DKLAPAQYIR YTPSQQGVAF NSGAKQRVIR MVEMQKDPME PPRFKINKKI PRGPP(SEP)PPAP VMH(SEP) PSRKM TVKEQQEWKI PPCISNWKNA KGYTIPLDKR LAADGRGLQT VHINENFAKL AEALYIADRK AREAVEMRAQ VERKM AQKE KEKHEEKLRE MAQKARERRA GIKTHVEKED GEARERDEIR HDRRKERQHD RNLSRAAPDK RSKLQRNENR DISEVI ALG VPNPRTSNEV QYDQRLFNQS KGMDSGFAGG EDEIYNVYDQ AWRGGKDMAQ SIYRPSKNLD KDMYGDDLEA RIKTNRF VP DKEFSGSDRR QRGREGPVQF EEDPFGLDKF LEEAKQHGGS KRPSDSSRPK EHEHEGKKRR KE

UniProtKB: SNW domain-containing protein 1

Macromolecule #19: Peptidyl-prolyl cis-trans isomerase-like 1

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase-like 1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.257805 KDa

Sequence

String:

MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPD LKFTGAGILA MANAGPDTNG SQFFVTLAPT QWLDGKHTIF GRVCQGIGMV NRVGMVETNS QDRPVDDVKI I KAYPSG

UniProtKB: Peptidyl-prolyl cis-trans isomerase-like 1

Macromolecule #20: Pleiotropic regulator 1

• Macromolecule: Name: Pleiotropic regulator 1 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 57.280758 KDa

Sequence

String:

MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT SKENLKEKGP QNATDSYVHK QYPANQGQE VEYFVAGTHP YPPGPGVALT ADTKIQRMPS ESAAQSLAVA LPLQTKADAN RTAPSGSEYR HPGASDRPQP T AMNSIVME TGNTKNSALM AKKAPTMPKP QWHPPWKLYR VISGHLGWVR CIAVEPGNQW FVTGSADRTI KIWDLASGKL KL SLTGHIS TVRGVIVSTR SPYLFSCGED KQVKCWDLEY NKVIRHYHGH LSAVYGLDLH PTIDVLVTCS RDSTARIWDV RTK ASVHTL SGHTNAVATV RCQAAEPQII TGSHDTTIRL WDLVAGKTRV TLTNHKKSVR AVVLHPRHYT FASGSPDNIK QWKF PDGSF IQNLSGHNAI INTLTVNSDG VLVSGADNGT MHLWDWRTGY NFQRVHAAVQ PGSLDSESGI FACAFDQSES RLLTA EADK TIKVYREDDT ATEETHPVSW KPEIIKRKRF

UniProtKB: Pleiotropic regulator 1

Macromolecule #21: RNA helicase aquarius

• Macromolecule: Name: RNA helicase aquarius / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 171.502453 KDa

Sequence

String:

MAAPAQPKKI VAPTVSQINA EFVTQLACKY WAPHIKKKSP FDIKVIEDIY EKEIVKSRFA IRKIMLLEFS QYLENYLWMN YSPEVSSKA YLMSICCMVN EKFRENVPAW EIFKKKPDHF PFFFKHILKA ALAETDGEFS LHEQTVLLLF LDHCFNSLEV D LIRSQVQQ LISLPMWMGL QLARLELELK KTPKLRKFWN LIKKNDEKMD PEAREQAYQE RRFLSQLIQK FISVLKSVPL SE PVTMDKV HYCERFIELM IDLEALLPTR RWFNTILDDS HLLVHCYLSN LVRREEDGHL FSQLLDMLKF YTGFEINDQT GNA LTENEM TTIHYDRITS LQRAAFAHFP ELYDFALSNV AEVDTRESLV KFFGPLSSNT LHQVASYLCL LPTLPKNEDT TFDK EFLLE LLVSRHERRI SQIQQLNQMP LYPTEKIIWD ENIVPTEYYS GEGCLALPKL NLQFLTLHDY LLRNFNLFRL ESTYE IRQD IEDSVSRMKP WQSEYGGVVF GGWARMAQPI VAFTVVEVAK PNIGENWPTR VRADVTINLN VRDHIKDEWE GLRKHD VCF LITVRPTKPY GTKFDRRRPF IEQVGLVYVR GCEIQGMLDD KGRVIEDGPE PRPNLRGESR TFRVFLDPNQ YQQDMTN TI QNGAEDVYET FNIIMRRKPK ENNFKAVLET IRNLMNTDCV VPDWLHDIIL GYGDPSSAHY SKMPNQIATL DFNDTFLS I EHLKASFPGH NVKVTVEDPA LQIPPFRITF PVRSGKGKKR KDADVEDEDT EEAKTLIVEP HVIPNRGPYP YNQPKRNTI QFTHTQIEAI RAGMQPGLTM VVGPPGTGKT DVAVQIISNI YHNFPEQRTL IVTHSNQALN QLFEKIMALD IDERHLLRLG HGEEELETE KDFSRYGRVN YVLARRIELL EEVKRLQKSL GVPGDASYTC ETAGYFFLYQ VMSRWEEYIS KVKNKGSTLP D VTEVSTFF PFHEYFANAP QPIFKGRSYE EDMEIAEGCF RHIKKIFTQL EEFRASELLR SGLDRSKYLL VKEAKIIAMT CT HAALKRH DLVKLGFKYD NILMEEAAQI LEIETFIPLL LQNPQDGFSR LKRWIMIGDH HQLPPVIKNM AFQKYSNMEQ SLF TRFVRV GVPTVDLDAQ GRARASLCNL YNWRYKNLGN LPHVQLLPEF STANAGLLYD FQLINVEDFQ GVGESEPNPY FYQN LGEAE YVVALFMYMC LLGYPADKIS ILTTYNGQKH LIRDIINRRC GNNPLIGRPN KVTTVDRFQG QQNDYILLSL VRTRA VGHL RDVRRLVVAM SRARLGLYIF ARVSLFQNCF ELTPAFSQLT ARPLHLHIIP TEPFPTTRKN GERPSHEVQI IKNMPQ MAN FVYNMYMHLI QTTHHYHQTL LQLPPAMVEE GEEVQNQETE LETEEEAMTV QADIIPSPTD TSCRQETPAF QTDTTPS ET GATSTPEAIP ALSETTPTVV GAVSAPAEAN TPQDATSAPE ETK

UniProtKB: RNA helicase aquarius

Macromolecule #22: Serine/arginine repetitive matrix protein 2

• Macromolecule: Name: Serine/arginine repetitive matrix protein 2 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 300.255312 KDa

Sequence

String:

MYNGIGLPTP RGSGTNGYVQ RNLSLVRGRR GERPDYKGEE ELRRLEAALV KRPNPDILDH ERKRRVELRC LELEEMMEEQ GYEEQQIQE KVATFRLMLL EKDVNPGGKE ETPGQRPAVT ETHQLAELNE KKNERLRAAF GISDSYVDGS SFDPQRRARE A KQPAPEPP KPYSLVRESS SSRSPTPKQK KKKKKKDRGR RSESSSPRRE RKKSSKKKKH RSESESKKRK HRSPTPKSKR KS KDKKRKR SRSTTPAPKS RRAHRSTSAD SASSSDTSRS RSRSAAAKTH TTALAGRSPS PASGRRGEGD APFSEPGTTS TQR PSSPET ATKQPSSPYE DKDKDKKEKS ATRPSPSPER SSTGPEPPAP TPLLAERHGG SPQPLATTPL SQEPVNPPSE ASPT RDRSP PKSPEKLPQS SSSESSPPSP QPTKVSRHAS SSPESPKPAP APGSHREISS SPTSKNRSHG RAKRDKSHSH TPSRR MGRS RSPATAKRGR SRSRTPTKRG HSRSRSPQWR RSRSAQRWGR SRSPQRRGRS RSPQRPGWSR SRNTQRRGRS RSARRG RSH SRSPATRGRS RSRTPARRGR SRSRTPARRR SRSRTPTRRR SRSRTPARRG RSRSRTPARR RSRTRSPVRR RSRSRSP AR RSGRSRSRTP ARRGRSRSRT PARRGRSRSR TPARRSGRSR SRTPARRGRS RSRTPRRGRS RSRSLVRRGR SHSRTPQR R GRSGSSSERK NKSRTSQRRS RSNSSPEMKK SRISSRRSRS LSSPRSKAKS RLSLRRSLSG SSPCPKQKSQ TPPRRSRSG SSQPKAKSRT PPRRSRSSSS PPPKQKSKTP SRQSHSSSSP HPKVKSGTPP RQGSITSPQA NEQSVTPQRR SCFESSPDPE LKSRTPSRH SCSGSSPPRV KSSTPPRQSP SRSSSPQPKV KAIISPRQRS HSGSSSPSPS RVTSRTTPRR SRSVSPCSNV E SRLLPRYS HSGSSSPDTK VKPETPPRQS HSGSISPYPK VKAQTPPGPS LSGSKSPCPQ EKSKDSLVQS CPGSLSLCAG VK SSTPPGE SYFGVSSLQL KGQSQTSPDH RSDTSSPEVR QSHSESPSLQ SKSQTSPKGG RSRSSSPVTE LASRSPIRQD RGE FSASPM LKSGMSPEQS RFQSDSSSYP TVDSNSLLGQ SRLETAESKE KMALPPQEDA TASPPRQKDK FSPFPVQDRP ESSL VFKDT LRTPPRERSG AGSSPETKEQ NSALPTSSQD EELMEVVEKS EEPAGQILSH LSSELKEMST SNFESSPEVE ERPAV SLTL DQSQSQASLE AVEVPSMASS WGGPHFSPEH KELSNSPLRE NSFGSPLEFR NSGPLGTEMN TGFSSEVKED LNGPFL NQL ETDPSLDMKE QSTRSSGHSS SELSPDAVEK AGMSSNQSIS SPVLDAVPRT PSRERSSSAS SPEMKDGLPR TPSRRSR SG SSPGLRDGSG TPSRHSLSGS SPGMKDIPRT PSRGRSECDS SPEPKALPQT PRPRSRSPSS PELNNKCLTP QRERSGSE S SVDQKTVART PLGQRSRSGS SQELDVKPSA SPQERSESDS SPDSKAKTRT PLRQRSRSGS SPEVDSKSRL SPRRSRSGS SPEVKDKPRA APRAQSGSDS SPEPKAPAPR ALPRRSRSGS SSKGRGPSPE GSSSTESSPE HPPKSRTARR GSRSSPEPKT KSRTPPRRR SSRSSPELTR KARLSRRSRS ASSSPETRSR TPPRHRRSPS VSSPEPAEKS RSSRRRRSAS SPRTKTTSRR G RSPSPKPR GLQRSRSRSR REKTRTTRRR DRSGSSQSTS RRRQRSRSRS RVTRRRRGGS GYHSRSPARQ ESSRTSSRRR RG RSRTPPT SRKRSRSRTS PAPWKRSRSR ASPATHRRSR SRTPLISRRR SRSRTSPVSR RRSRSRTSVT RRRSRSRASP VSR RRSRSR TPPVTRRRSR SRTPTTRRRS RSRTPPVTRR RSRSRTPPVT RRRSRSRTSP ITRRRSRSRT SPVTRRRSRS RTSP VTRRR SRSRTSPVTR RRSRSRTPPA IRRRSRSRTP LLPRKRSRSR SPLAIRRRSR SRTPRTARGK RSLTRSPPAI RRRSA SGSS SDRSRSATPP ATRNHSGSRT PPVALNSSRM SCFSRPSMSP TPLDRCRSPG MLEPLGSSRT PMSVLQQAGG SMMDGP GPR IPDHQRTSVP ENHAQSRIAL ALTAISLGTA RPPPSMSAAG LAARMSQVPA PVPLMSLRTA PAANLASRIP AASAAAM NL ASARTPAIPT AVNLADSRTP AAAAAMNLAS PRTAVAPSAV NLADPRTPTA PAVNLAGART PAALAALSLT GSGTPPTA A NYPSSSRTPQ APASANLVGP RSAHATAPVN IAGSRTAAAL APASLTSARM APALSGANLT SPRVPLSAYE RVSGRTSPP LLDRARSRTP PSAPSQSRMT SERAPSPSSR MGQAPSQSLL PPAQDQPRSP VPSAFSDQSR CLIAQTTPVA GSQSLSSGAV ATTTSSAGD HNGMLSVPAP GVPHSDVGEP PASTGAQQPS ALAALQPAKE RRSSSSSSSS SSSSSSSSSS SSSSSSSGSS S SDSEGSSL PVQPEVALKR VPSPTPAPKE AVREGRPPEP TPAKRKRRSS SSSSSSSSSS SSSSSSSSSS SSSSSSSSSS SS SSSSSSS SPSPAKPGPQ ALPKPASPKK PPPGERRSRS PRKPIDSLRD SRSLSYSPVE RRRPSPQPSP RDQQSSSSER GSR RGQRGD SRSPSHKRRR ETPSPRPMRH RSSRSP

UniProtKB: Serine/arginine repetitive matrix protein 2

Macromolecule #23: Pre-mRNA-splicing factor CWC22 homolog

• Macromolecule: Name: Pre-mRNA-splicing factor CWC22 homolog / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 105.646578 KDa

Sequence

String:

MKSSVAQIKP SSGHDRRENL NSYQRNSSPE DRYEEQERSP RDRDYFDYSR SDYEHSRRGR SYDSSMESRN RDREKRRERE RDTDRKRSR KSPSPGRRNP ETSVTQSSSA QDEPATKKKK DELDPLLTRT GGAYIPPAKL RMMQEQITDK NSLAYQRMSW E ALKKSING LINKVNISNI SIIIQELLQE NIVRGRGLLS RSVLQAQSAS PIFTHVYAAL VAIINSKFPQ IGELILKRLI LN FRKGYRR NDKQLCLTAS KFVAHLINQN VAHEVLCLEM LTLLLERPTD DSVEVAIGFL KECGLKLTQV SPRGINAIFE RLR NILHES EIDKRVQYMI EVMFAVRKDG FKDHPIILEG LDLVEEDDQF THMLPLEDDY NPEDVLNVFK MDPNFMENEE KYKA IKKEI LDEGDTDSNT DQDAGSSEED EEEEEEEGEE DEEGQKVTIH DKTEINLVSF RRTIYLAIQS SLDFEECAHK LLKME FPES QTKELCNMIL DCCAQQRTYE KFFGLLAGRF CMLKKEYMES FEGIFKEQYD TIHRLETNKL RNVAKMFAHL LYTDSL PWS VLECIKLSEE TTTSSSRIFV KIFFQELCEY MGLPKLNARL KDETLQPFFE GLLPRDNPRN TRFAINFFTS IGLGGLT DE LREHLKNTPK VIVAQKPDVE QNKSSPSSSS SASSSSESDS SDSDSDSSDS SSESSSEESD SSSISSHSSA SANDVRKK G HGKTRSKEVD KLIRNQQTND RKQKERRQEH GHQETRTERE RRSEKHRDQN SSGSNWRDPI TKYTSDKDVP SERNNYSRV ANDRDQEMHI DLENKHGDPK KKRGERRNSF SENEKHTHRI KDSENFRRKD RSKSKEMNRK HSGSRSDEDR YQNGAERRWE KSSRYSEQS RESKKNQDRR REKSPAKQK

UniProtKB: Pre-mRNA-splicing factor CWC22 homolog

Macromolecule #24: Pre-mRNA-processing factor 17

• Macromolecule: Name: Pre-mRNA-processing factor 17 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 65.61218 KDa

Sequence

String:

MSAAIAALAA SYGSGSGSES DSDSESSRCP LPAADSLMHL TKSPSSKPSL AVAVDSAPEV AVKEDLETGV HLDPAVKEVQ YNPTYETMF APEFGPENPF RTQQMAAPRN MLSGYAEPAH INDFMFEQQR RTFATYGYAL DPSLDNHQVS AKYIGSVEEA E KNQGLTVF ETGQKKTEKR KKFKENDASN IDGFLGPWAK YVDEKDVAKP SEEEQKELDE ITAKRQKKGK QEEEKPGEEK TI LHVKEMY DYQGRSYLHI PQDVGVNLRS TMPPEKCYLP KKQIHVWSGH TKGVSAVRLF PLSGHLLLSC SMDCKIKLWE VYG ERRCLR TFIGHSKAVR DICFNTAGTQ FLSAAYDRYL KLWDTETGQC ISRFTNRKVP YCVKFNPDED KQNLFVAGMS DKKI VQWDI RSGEIVQEYD RHLGAVNTIV FVDENRRFVS TSDDKSLRVW EWDIPVDFKY IAEPSMHSMP AVTLSPNGKW LACQS MDNQ ILIFGAQNRF RLNKKKIFKG HMVAGYACQV DFSPDMSYVI SGDGNGKLNI WDWKTTKLYS RFKAHDKVCI GAVWHP HET SKVITCGWDG LIKLWD

UniProtKB: Pre-mRNA-processing factor 17

Macromolecule #25: Pre-mRNA-splicing factor CWC25 homolog

• Macromolecule: Name: Pre-mRNA-splicing factor CWC25 homolog / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 49.769938 KDa

Sequence

String:

MGGGDLNLKK SWHPQTLRNV EKVWKAEQKH EAERKKIEEL QRELREERAR EEMQRYAEDV GAVKKKEEKL DWMYQGPGGM VNRDEYLLG RPIDKYVFEK MEEKEAGCSS ETGLLPGSIF APSGANSLLD MASKIREDPL FIIRKKEEEK KREVLNNPVK M KKIKELLQ MSLEKKEKKK KKEKKKKHKK HKHRSSSSDR SSSEDEHSAG RSQKKMANSS PVLSKVPGYG LQVRNSDRNQ GL QGPLTAE QKRGHGMKNH SRSRSSSHSP PRHASKKSTR EAGSRDRRSR SLGRRSRSPR PSKLHNSKVN RRETGQTRSP SPK KEVYQR RHAPGYTRKL SAEELERKRQ EMMENAKWRE EERLNILKRH AKDEEREQRL EKLDSRDGKF IHRMKLESAS TSSL EDRVK RNIYSLQRTS VALEKNFMKR

UniProtKB: Pre-mRNA-splicing factor CWC25 homolog

Macromolecule #26: Coiled-coil domain-containing protein 94

• Macromolecule: Name: Coiled-coil domain-containing protein 94 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.141816 KDa

Sequence

String:

MSERKVLNKY YPPDFDPSKI PKLKLPKDRQ YVVRLMAPFN MRCKTCGEYI YKGKKFNARK ETVQNEVYLG LPIFRFYIKC TRCLAEITF KTDPENTDYT MEHGATRNFQ AEKLLEEEEK RVQKEREDEE LNNPMKVLEN RTKDSKLEME VLENLQELKD L NQRQAHVD FEAMLRQHRL SEEERRRQQQ EEDEQETAAL LEEARKRRLL EDSDSEDEAA PSPLQPALRP NPTAILDEAP KP KRKVEVW EQSVGSLGSR PPLSRLVVVK KAKADPDCSN GQPQAAPTPG APQNRKEANP TPLTPGASSL SQLGAYLDSD DSN GSN

UniProtKB: Splicing factor YJU2

Macromolecule #27: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

• Macromolecule: Name: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16; type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 140.720641 KDa

Sequence

String:

MGDTSEDASI HRLEGTDLDC QVGGLICKSK SAASEQHVFK APAPRPSLLG LDLLASLKRR EREEKDDGED KKKSKVSSYK DWEESKDDQ KDAEEEGGDQ AGQNIRKDRH YRSARVETPS HPGGVSEEFW ERSRQRERER REHGVYASSK EEKDWKKEKS R DRDYDRKR DRDERDRSRH SSRSERDGGS ERSSRRNEPE SPRHRPKDAA TPSRSTWEEE DSGYGSSRRS QWESPSPTPS YR DSERSHR LSTRDRDRSV RGKYSDDTPL PTPSYKYNEW ADDRRHLGST PRLSRGRGRR EEGEEGISFD TEEERQQWED DQR QADRDW YMMDEGYDEF HNPLAYSSED YVRRREQHLH KQKQKRISAQ RRQINEDNER WETNRMLTSG VVHRLEVDED FEED NAAKV HLMVHNLVPP FLDGRIVFTK QPEPVIPVKD ATSDLAIIAR KGSQTVRKHR EQKERKKAQH KHWELAGTKL GDIMG VKKE EEPDKAVTED GKVDYRTEQK FADHMKRKSE ASSEFAKKKS ILEQRQYLPI FAVQQELLTI IRDNSIVIVV GETGSG KTT QLTQYLHEDG YTDYGMIGCT QPRRVAAMSV AKRVSEEMGG NLGEEVGYAI RFEDCTSENT LIKYMTDGIL LRESLRE AD LDHYSAIIMD EAHERSLNTD VLFGLLREVV ARRSDLKLIV TSATMDAEKF AAFFGNVPIF HIPGRTFPVD ILFSKTPQ E DYVEAAVKQS LQVHLSGAPG DILIFMPGQE DIEVTSDQIV EHLEELENAP ALAVLPIYSQ LPSDLQAKIF QKAPDGVRK CIVATNIAET SLTVDGIMFV IDSGYCKLKV FNPRIGMDAL QIYPISQANA NQRSGRAGRT GPGQCFRLYT QSAYKNELLT TTVPEIQRT NLANVVLLLK SLGVQDLLQF HFMDPPPEDN MLNSMYQLWI LGALDNTGGL TSTGRLMVEF PLDPALSKML I VSCDMGCS SEILLIVSML SVPAIFYRPK GREEESDQIR EKFAVPESDH LTYLNVYLQW KNNNYSTIWC NDHFIHAKAM RK VREVRAQ LKDIMVQQRM SLASCGTDWD IVRKCICAAY FHQAAKLKGI GEYVNIRTGM PCHLHPTSSL FGMGYTPDYI VYH ELVMTT KEYMQCVTAV DGEWLAELGP MFYSVKQAGK SRQENRRRAK EEASAMEEEM ALAEEQLRAR RQEQEKRSPL GSVR STKIY TPGRKEQGEP MTPRRTPARF GL

UniProtKB: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

Macromolecule #28: Pre-mRNA-processing factor 19

• Macromolecule: Name: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 28 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 55.245547 KDa

Sequence

String:

MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE QLIDIKVAHP IRPKPPSATS IPAILKALQD EWDAVMLHS FTLRQQLQTT RQELSHALYQ HDAACRVIAR LTKEVTAARE ALATLKPQAG LIVPQAVPSS QPSVVGAGEP M DLGELVGM TPEIIQKLQD KATVLTTERK KRGKTVPEEL VKPEELSKYR QVASHVGLHS ASIPGILALD LCPSDTNKIL TG GADKNVV VFDKSSEQIL ATLKGHTKKV TSVVFHPSQD LVFSASPDAT IRIWSVPNAS CVQVVRAHES AVTGLSLHAT GDY LLSSSD DQYWAFSDIQ TGRVLTKVTD ETSGCSLTCA QFHPDGLIFG TGTMDSQIKI WDLKERTNVA NFPGHSGPIT SIAF SENGY YLATAADDSS VKLWDLRKLK NFKTLQLDNN FEVKSLIFDQ SGTYLALGGT DVQIYICKQW TEILHFTEHS GLTTG VAFG HHAKFIASTG MDRSLKFYSL

UniProtKB: Pre-mRNA-processing factor 19

Macromolecule #29: Eukaryotic initiation factor 4A-III

• Macromolecule: Name: Eukaryotic initiation factor 4A-III / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 46.930961 KDa

Sequence

String:

MATTATMATS GSARKRLLKE EDMTKVEFET SEEVDVTPTF DTMGLREDLL RGIYAYGFEK PSAIQQRAIK QIIKGRDVIA QSQSGTGKT ATFSISVLQC LDIQVRETQA LILAPTRELA VQIQKGLLAL GDYMNVQCHA CIGGTNVGED IRKLDYGQHV V AGTPGRVF DMIRRRSLRT RAIKMLVLDE ADEMLNKGFK EQIYDVYRYL PPATQVVLIS ATLPHEILEM TNKFMTDPIR IL VKRDELT LEGIKQFFVA VEREEWKFDT LCDLYDTLTI TQAVIFCNTK RKVDWLTEKM REANFTVSSM HGDMPQKERE SIM KEFRSG ASRVLISTDV WARGLDVPQV SLIINYDLPN NRELYIHRIG RSGRYGRKGV AINFVKNDDI RILRDIEQYY STQI DEMPM NVADLI

UniProtKB: Eukaryotic initiation factor 4A-III

Macromolecule #30: Protein mago nashi homolog

• Macromolecule: Name: Protein mago nashi homolog / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.189625 KDa

Sequence

String:

MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELE IVIGDEHISF TTSKIGSLID VNQSKDPEGL RVFYYLVQDL KCLVFSLIGL HFKIKPI

UniProtKB: Protein mago nashi homolog

Macromolecule #31: RNA-binding protein 8A

• Macromolecule: Name: RNA-binding protein 8A / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 19.92507 KDa

Sequence

String:

MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDI HDKFAEYGEI KNIHLNLDRR TGYLKGYTLV EYETYKEAQA AMEGLNGQDL MGQPISVDWC FVRGPPKGKR R GGRRRSRS PDRRRR

UniProtKB: RNA-binding protein 8A

Macromolecule #32: Protein CASC3

• Macromolecule: Name: Protein CASC3 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 76.381992 KDa

Sequence

String:

MADRRRQRAS QDTEDEESGA SGSDSGGSPL RGGGSCSGSA GGGGSGSLPS QRGGRTGALH LRRVESGGAK SAEESECESE DGIEGDAVL SDYESAEDSE GEEGEYSEEE NSKVELKSEA NDAVNSSTKE EKGEEKPDTK STVTGERQSG DGQESTEPVE N KVGKKGPK HLDDDEDRKN PAYIPRKGLF FEHDLRGQTQ EEEVRPKGRQ RKLWKDEGRW EHDKFREDEQ APKSRQELIA LY GYDIRSA HNPDDIKPRR IRKPRYGSPP QRDPNWNGER LNKSHRHQGL GGTLPPRTFI NRNAAGTGRM SAPRNYSRSG GFK EGRAGF RPVEAGGQHG GRSGETVKHE ISYRSRRLEQ TSVRDPSPEA DAPVLGSPEK EEAASEPPAA APDAAPPPPD RPIE KKSYS RARRTRTKVG DAVKLAEEVP PPPEGLIPAP PVPETTPTPP TKTGTWEAPV DSSTSGLEQD VAQLNIAEQN WSPGQ PSFL QPRELRGMPN HIHMGAGPPP QFNRMEEMGV QGGRAKRYSS QRQRPVPEPP APPVHISIME GHYYDPLQFQ GPIYTH GDS PAPLPPQGML VQPGMNLPHP GLHPHQTPAP LPNPGLYPPP VSMSPGQPPP QQLLAPTYFS APGVMNFGNP SYPYAPG AL PPPPPPHLYP NTQAPSQVYG GVTYYNPAQQ QVQPKPSPPR RTPQPVTIKP PPPEVVSRGS S

UniProtKB: Protein CASC3

Macromolecule #33: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.940308 KDa

Sequence

String:

MSIGVPIKVL HEAEGHIVTC ETNTGEVYRG KLIEAEDNMN CQMSNITVTY RDGRVAQLEQ VYIRGSKIRF LILPDMLKNA PMLKSMKNK NQGSGAGRGK AAILKAQVAA RGRGRGMGRG NIFQKRR

UniProtKB: Small nuclear ribonucleoprotein Sm D3

Macromolecule #34: Small nuclear ribonucleoprotein-associated proteins B and B'

• Macromolecule: Name: Small nuclear ribonucleoprotein-associated proteins B and B'; type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.642131 KDa

Sequence

String:

MTVGKSSKML QHIDYRMRCI LQDGRIFIGT FKAFDKHMNL ILCDCDEFRK IKPKNSKQAE REEKRVLGLV LLRGENLVSM TVEGPPPKD TGIARVPLAG AAGGPGIGRA AGRGIPAGVP MPQAPAGLAG PVRGVGGPSQ QVMTPQGRGT VAAAAAAATA S IAGAPTQY PPGRGGPPPP MGRGAPPPGM MGPPPGMRPP MGPPMGIPPG RGTPMGMPPP GMRPPPPGMR GPPPPGMRPP RP

UniProtKB: Small nuclear ribonucleoprotein-associated proteins B and B'

Macromolecule #35: Small nuclear ribonucleoprotein Sm D1

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.310653 KDa

Sequence

String:

MKLVRFLMKL SHETVTIELK NGTQVHGTIT GVDVSMNTHL KAVKMTLKNR EPVQLETLSI RGNNIRYFIL PDSLPLDTLL VDVEPKVKS KKREAVAGRG RGRGRGRGRG RGRGRGGPRR

UniProtKB: Small nuclear ribonucleoprotein Sm D1

Macromolecule #36: Small nuclear ribonucleoprotein Sm D2

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 36 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.551928 KDa

Sequence

String:

MSLLNKPKSE MTPEELQKRE EEEFNTGPLS VLTQSVKNNT QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKS GKGKKKSKP VNKDRYISKM FLRGDSVIVV LRNPLIAGK

UniProtKB: Small nuclear ribonucleoprotein Sm D2

Macromolecule #37: Small nuclear ribonucleoprotein F

• Macromolecule: Name: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 37 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.734171 KDa

Sequence

String:

MSLPLNPKPF LNGLTGKPVM VKLKWGMEYK GYLVSVDGYM NMQLANTEEY IDGALSGHLG EVLIRCNNVL YIRGVEEEEE DGEMRE

UniProtKB: Small nuclear ribonucleoprotein F

Macromolecule #38: Small nuclear ribonucleoprotein E

• Macromolecule: Name: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 38 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.817601 KDa

Sequence

String:

MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQS VSN

UniProtKB: Small nuclear ribonucleoprotein E

Macromolecule #39: Small nuclear ribonucleoprotein G

• Macromolecule: Name: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 39 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.508084 KDa

Sequence

String:

MSKAHPPELK KFMDKKLSLK LNGGRHVQGI LRGFDPFMNL VIDECVEMAT SGQQNNIGMV VIRGNSIIML EALERV

UniProtKB: Small nuclear ribonucleoprotein G

Macromolecule #40: U2 small nuclear ribonucleoprotein A'

• Macromolecule: Name: U2 small nuclear ribonucleoprotein A' / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.456584 KDa

Sequence

String:

MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPC LTELILTNNS LVELGDLDPL ASLKSLTYLS ILRNPVTNKK HYRLYVIYKV PQVRVLDFQK VKLKERQEAE K MFKGKRGA QLAKDIARRS KTFNPGAGLP TDKKKGGPSP GDVEAIKNAI ANASTLAEVE RLKGLLQSGQ IPGRERRSGP TD DGEEEME EDTVTNGS

UniProtKB: U2 small nuclear ribonucleoprotein A'

Macromolecule #41: U2 small nuclear ribonucleoprotein B''

• Macromolecule: Name: U2 small nuclear ribonucleoprotein B'' / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 25.524367 KDa

Sequence

String:

MDIRPNHTIY INNMNDKIKK EELKRSLYAL FSQFGHVVDI VALKTMKMRG QAFVIFKELG SSTNALRQLQ GFPFYGKPMR IQYAKTDSD IISKMRGTFA DKEKKKEKKK AKTVEQTATT TNKKPGQGTP NSANTQGNST PNPQVPDYPP NYILFLNNLP E ETNEMMLS MLFNQFPGFK EVRLVPGRHD IAFVEFENDG QAGAARDALQ GFKITPSHAM KITYAKK

UniProtKB: U2 small nuclear ribonucleoprotein B''

Macromolecule #42: Peptidyl-prolyl cis-trans isomerase E

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase E / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.475773 KDa

Sequence

String:

MATTKRVLYV GGLAEEVDDK VLHAAFIPFG DITDIQIPLD YETEKHRGFA FVEFELAEDA AAAIDNMNES ELFGRTIRVN LAKPMRIKE GSSRPVWSDD DWLKKFSGKT LEENKEEEGS EPPKAETQEG EPIAKKARSN PQVYMDIKIG NKPAGRIQML L RSDVVPMT AENFRCLCTH EKGFGFKGSS FHRIIPQFMC QGGDFTNHNG TGGKSIYGKK FDDENFILKH TGPGLLSMAN SG PNTNGSQ FFLTCDKTDW LDGKHVVFGE VTEGLDVLRQ IEAQGSKDGK PKQKVIIADC GEYV

UniProtKB: Peptidyl-prolyl cis-trans isomerase E

Macromolecule #43: Pre-mRNA-splicing factor ISY1 homolog

• Macromolecule: Name: Pre-mRNA-splicing factor ISY1 homolog / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.046254 KDa

Sequence

String:

MARNAEKAMT ALARFRQAQL EEGKVKERRP FLASECTELP KAEKWRRQII GEISKKVAQI QNAGLGEFRI RDLNDEINKL LREKGHWEV RIKELGGPDY GKVGPKMLDH EGKEVPGNRG YKYFGAAKDL PGVRELFEKE PLPPPRKTRA ELMKAIDFEY Y GYLDEDDG VIVPLEQEYE KKLRAELVEK WKAEREARLA RGEKEEEEEE EEEINIYAVT EEESDEEGSQ EKGGDDSQQK FI AHVPVPS QQEIEEALVR RKKMELLQKY ASETLQAQSE EARRLLGY

UniProtKB: Pre-mRNA-splicing factor ISY1 homolog

Macromolecule #44: Peptidylprolyl isomerase domain and WD repeat-containing protein 1

• Macromolecule: Name: Peptidylprolyl isomerase domain and WD repeat-containing protein 1; type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 73.679891 KDa

Sequence

String:

MAAESGSDFQ QRRRRRRDPE EPEKTELSER ELAVAVAVSQ ENDEENEERW VGPLPVEATL AKKRKVLEFE RVYLDNLPSA SMYERSYMH RDVITHVVCT KTDFIITASH DGHVKFWKKI EEGIEFVKHF RSHLGVIESI AVSSEGALFC SVGDDKAMKV F DVVNFDMI NMLKLGYFPG QCEWIYCPGD AISSVAASEK STGKIFIYDG RGDNQPLHIF DKLHTSPLTQ IRLNPVYKAV VS SDKSGMI EYWTGPPHEY KFPKNVNWEY KTDTDLYEFA KCKAYPTSVC FSPDGKKIAT IGSDRKVRIF RFVTGKLMRV FDE SLSMFT ELQQMRQQLP DMEFGRRMAV ERELEKVDAV RLINIVFDET GHFVLYGTML GIKVINVETN RCVRILGKQE NIRV MQLAL FQGIAKKHRA ATTIEMKASE NPVLQNIQAD PTIVCTSFKK NRFYMFTKRE PEDTKSADSD RDVFNEKPSK EEVMA ATQA EGPKRVSDSA IIHTSMGDIH TKLFPVECPK TVENFCVHSR NGYYNGHTFH RIIKGFMIQT GDPTGTGMGG ESIWGG EFE DEFHSTLRHD RPYTLSMANA GSNTNGSQFF ITVVPTPWLD NKHTVFGRVT KGMEVVQRIS NVKVNPKTDK PYEDVSI IN ITVK

UniProtKB: Peptidylprolyl isomerase domain and WD repeat-containing protein 1

Macromolecule #45: Corepressor interacting with RBPJ 1

• Macromolecule: Name: Corepressor interacting with RBPJ 1 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 52.44175 KDa

Sequence

String:

MGKSFANFMC KKDFHPASKS NIKKVWMAEQ KISYDKKKQE ELMQQYLKEQ ESYDNRLLMG DERVKNGLNF MYEAPPGAKK ENKEKEETE GETEYKFEWQ KGAPREKYAK DDMNIRDQPF GIQVRNVRCI KCHKWGHVNT DRECPLFGLS GINASSVPTD G SGPSMHPS ELIAEMRNSG FALKRNVLGR NLTANDPSQE YVASEGEEDP EVEFLKSLTT KQKQKLLRKL DRLEKKKKKK DR KKKKFQK SRSKHKKHKS SSSSSSSSSS SSSTETSESS SESESNNKEK KIQRKKRKKN KCSGHNNSDS EEKDKSKKRK LHE ELSSSH HNREKAKEKP RFLKHESSRE DSKWSHSDSD KKSRTHKHSP EKRGSERKEG SSRSHGREER SRRSRSRSPG SYKQ RETRK RAQRNPGEEQ SRRNDSRSHG TDLYRGEKMY REHPGGTHTK VTQRE

UniProtKB: Corepressor interacting with RBPJ 1

Macromolecule #46: WD repeat-containing protein 70

• Macromolecule: Name: WD repeat-containing protein 70 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 73.306172 KDa

Sequence

String:

MERSGPSEVT GSDASGPDPQ LAVTMGFTGF GKKARTFDLE AMFEQTRRTA VERSRKTLEA REKEEEMNRE KELRRQNEDI EPTSSRSNV VRDCSKSSSR DTSSSESEQS SDSSDDELIG PPLPPKMVGK PVNFMEEDIL GPLPPPLNEE EEEAEEEEEE E EEEENPVH KIPDSHEITL KHGTKTVSAL GLDPSGARLV TGGYDYDVKF WDFAGMDASF KAFRSLQPCE CHQIKSLQYS NT GDMILVV SGSSQAKVID RDGFEVMECI KGDQYIVDMA NTKGHTAMLH TGSWHPKIKG EFMTCSNDAT VRTWEVENPK KQK SVFKPR TMQGKKVIPT TCTYSRDGNL IAAACQNGSI QIWDRNLTVH PKFHYKQAHD SGTDTSCVTF SYDGNVLASR GGDD SLKLW DIRQFNKPLF SASGLPTMFP MTDCCFSPDD KLIVTGTSIQ RGCGSGKLVF FERRTFQRVY EIDITDASVV RCLWH PKLN QIMVGTGNGL AKVYYDPNKS QRGAKLCVVK TQRKAKQAET LTQDYIITPH ALPMFREPRQ RSTRKQLEKD RLDPLK SHK PEPPVAGPGR GGRVGTHGGT LSSYIVKNIA LDKTDDSNPR EAILRHAKAA EDSPYWVSPA YSKTQPKTMF AQVESDD EE AKNEPEWKKR KI

UniProtKB: WD repeat-containing protein 70

Macromolecule #47: Protein FRG1

• Macromolecule: Name: Protein FRG1 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.227492 KDa

Sequence

String:

MAEYSYVKST KLVLKGTKTK SKKKKSKDKK RKREEDEETQ LDIVGIWWTV TNFGEISGTI AIEMDKGTYI HALDNGLFTL GAPHKEVDE GPSPPEQFTA VKLSDSRIAL KSGYGKYLGI NSDGLVVGRS DAIGPREQWE PVFQNGKMAL LASNSCFIRC N EAGDIEAK SKTAGEEEMI KIRSCAERET KKKDDIPEED KGNVKQCEIN YVKKFQSFQD HKLKISKEDS KILKKARKDG FL HETLLDR RAKLKADRYC K

UniProtKB: Protein FRG1

Macromolecule #48: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 3.178905 KDa

Sequence

String:

(UNK)(UNK)(UNK)P(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #2: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.254855 KDa

Sequence

String:

AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAU UUUUUGAGGC CUUGCUUUGG CAAGGCUA

GENBANK: GENBANK: AC105129.4

Macromolecule #6: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 6 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.404438 KDa

Sequence

String:

GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACACG CAAAUUCGU GAAGCGUUCC AUAUUUUU

Macromolecule #7: pre-mRNA

• Macromolecule: Name: pre-mRNA / type: rna / ID: 7 / Number of copies: 1
• Source (natural): Organism: unidentified adenovirus
• Molecular weight: Theoretical: 14.72978 KDa

Sequence

String:

GAUCGGGCUC GCCACUCUUG GAUCGGAACC CGUCGGCCUC CGAACG

Macromolecule #8: pre-mRNA

• Macromolecule: Name: pre-mRNA / type: rna / ID: 8 / Number of copies: 1
• Source (natural): Organism: unidentified adenovirus
• Molecular weight: Theoretical: 55.660688 KDa

Sequence

String:

GUAAGAGCCU AGCAUGUAGA ACUGGUUACC UGCAGCCCAA GCUUGCUGCA CGUCUAGGGC GCAGUAGUCC AGGGUUUCCU UGAUGAUGU CAUACUUAUC CUGUCCCUUU UUUUUCCACA GCUCGCGGUU GAGGACAAAC UCUUCGCGGU CUUUCCAGUG G GGAUCCGA UAUCCG

Macromolecule #9: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 9 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 60.186445 KDa

Sequence

String:

AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAU AUUAAAUGGA UUUUUGGAGC AGGGAGAUGG AAUAGGAGCU UGCUCCGUCC ACUCCACGCA UCGACCUGGU A UUGCAGUA CCUCCAGGAA CGGUGCACCC

GENBANK: GENBANK: K02227.1

Macromolecule #49: INOSITOL HEXAKISPHOSPHATE

• Macromolecule: Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 49 / Number of copies: 1 / Formula: IHP
• Molecular weight: Theoretical: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

Macromolecule #50: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 50 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #51: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 51 / Number of copies: 5 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #52: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 52 / Number of copies: 7 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.9
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.4000000000000001 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: INSILICO MODEL
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 18223
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD