- EMDB-34821: A cryo-EM structure of B. oleracea RNA polymerase V elongation co... -
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基本情報
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データベース: EMDB / ID: EMD-34821
タイトル
A cryo-EM structure of B. oleracea RNA polymerase V elongation complex at 2.73 Angstrom
マップデータ
試料
複合体: DNA-directed RNA polymerase V elongation complex
複合体: DNA-directed RNA polymerase
タンパク質・ペプチド: x 10種
複合体: DNA-RNA
DNA: x 2種
RNA: x 1種
リガンド: x 2種
キーワード
DNA-dependent RNA polymerase V / TRANSCRIPTION
機能・相同性
機能・相同性情報
RNA polymerase IV complex / RNA polymerase V complex / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / RNA polymerase I complex / RNA polymerase III complex / transcription-coupled nucleotide-excision repair / RNA polymerase III activity / RNA polymerase II, core complex / tRNA transcription by RNA polymerase III / RNA polymerase I activity ...RNA polymerase IV complex / RNA polymerase V complex / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / RNA polymerase I complex / RNA polymerase III complex / transcription-coupled nucleotide-excision repair / RNA polymerase III activity / RNA polymerase II, core complex / tRNA transcription by RNA polymerase III / RNA polymerase I activity / RNA polymerase II activity / transcription initiation at RNA polymerase II promoter / DNA-directed 5'-3' RNA polymerase activity / nucleic acid binding / protein dimerization activity / nucleolus / DNA binding / zinc ion binding 類似検索 - 分子機能
ジャーナル: Science / 年: 2023 タイトル: Structure and mechanism of the plant RNA polymerase V. 著者: Guohui Xie / Xuan Du / Hongmiao Hu / Sisi Li / Xiaofeng Cao / Steven E Jacobsen / Jiamu Du / 要旨: In addition to the conserved RNA polymerases I to III (Pols I to III) in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce noncoding RNA in the RNA-directed DNA methylation ...In addition to the conserved RNA polymerases I to III (Pols I to III) in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce noncoding RNA in the RNA-directed DNA methylation pathway in plants. Here, we report on the structures of cauliflower Pol V in the free and elongation conformations. A conserved tyrosine residue of NRPE2 stacks with a double-stranded DNA branch of the transcription bubble to potentially attenuate elongation by inducing transcription stalling. The nontemplate DNA strand is captured by NRPE2 to enhance backtracking, thereby increasing 3'-5' cleavage, which likely underpins Pol V's high fidelity. The structures also illuminate the mechanism of Pol V transcription stalling and enhanced backtracking, which may be important for Pol V's retention on chromatin to serve its function in tethering downstream factors for RNA-directed DNA methylation.