National Natural Science Foundation of China (NSFC)
91957201
中国
National Natural Science Foundation of China (NSFC)
31821091
中国
National Natural Science Foundation of China (NSFC)
31870833
中国
引用
ジャーナル: Nitric Oxide / 年: 2023 タイトル: NO binds to the distal site of haem in the fully activated soluble guanylate cyclase. 著者: Rui Liu / Yunlu Kang / Lei Chen / 要旨: Soluble guanylate cyclase (sGC) is the primary receptor for nitric oxide (NO). The binding of NO to the haem of sGC induces a large conformational change in the enzyme and activates its cyclase ...Soluble guanylate cyclase (sGC) is the primary receptor for nitric oxide (NO). The binding of NO to the haem of sGC induces a large conformational change in the enzyme and activates its cyclase activity. However, whether NO binds to the proximal site or the distal site of haem in the fully activated state remains under debate. Here, we present cryo-EM maps of sGC in the NO-activated state at high resolutions, allowing the observation of the density of NO. These cryo-EM maps show the binding of NO to the distal site of haem in the NO-activated state.