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- EMDB-34159: structure of human connexin 40.1 side-by-side decamer channel by ... -
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Open data
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Basic information
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Title | structure of human connexin 40.1 side-by-side decamer channel by cryoEM | ||||||||||||
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![]() | connexin side-by-side decamer channel / MEMBRANE PROTEIN | ||||||||||||
Function / homology | ![]() regulation of satellite cell activation involved in skeletal muscle regeneration / connexin complex / Gap junction assembly / gap junction channel activity / cell-cell signaling Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.9 Å | ||||||||||||
![]() | Zhang H / Wang DP | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: structure of human connexin 40.1 side-by-side decamer channel by cryoEM Authors: Zhang H / Wang DP | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 203.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.9 KB 12.9 KB | Display Display | ![]() |
Images | ![]() | 86.9 KB | ||
Others | ![]() ![]() | 200.7 MB 200.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8gnbMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.92 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_34159_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_34159_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : structure of human connexin 40.1 side-by-side decamer channel
Entire | Name: structure of human connexin 40.1 side-by-side decamer channel |
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Components |
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-Supramolecule #1: structure of human connexin 40.1 side-by-side decamer channel
Supramolecule | Name: structure of human connexin 40.1 side-by-side decamer channel type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 260 KDa |
-Macromolecule #1: Gap junction delta-4 protein
Macromolecule | Name: Gap junction delta-4 protein / type: protein_or_peptide / ID: 1 / Number of copies: 10 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 26.030973 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MEGVDLLGFL IITLNCNVTM VGKLWFVLTM LLRMLVIVLA GRPVYQDEQE RFVCNTLQPG CANVCYDVFS PVSHLRFWLI QGVCVLLPS AVFSVYVLHR GATLAALGPR RCPDPREPAS GQRRCPRPFG ERGGLQVPDF SAGYIIHLLL RTLLEAAFGA L HYFLFGFL ...String: MEGVDLLGFL IITLNCNVTM VGKLWFVLTM LLRMLVIVLA GRPVYQDEQE RFVCNTLQPG CANVCYDVFS PVSHLRFWLI QGVCVLLPS AVFSVYVLHR GATLAALGPR RCPDPREPAS GQRRCPRPFG ERGGLQVPDF SAGYIIHLLL RTLLEAAFGA L HYFLFGFL APKKFPCTRP PCTGVVDCYV SRPTEKSLLM LFLWAVSALS FLLGLADLVC SLRRRMRRRP GPPTS UniProtKB: Gap junction delta-4 protein |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 4 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: OTHER |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 106770 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |