+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-33995 | |||||||||
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Title | apo-ADGRG2 coupled to Gs | |||||||||
Map data | apo-ADGRG2-Gs complex | |||||||||
Sample |
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Function / homology | Function and homology information G protein-coupled receptor activity / adenylate cyclase-activating G protein-coupled receptor signaling pathway / Olfactory Signaling Pathway / Activation of the phototransduction cascade / Prostacyclin signalling through prostacyclin receptor / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / Glucagon signaling in metabolic regulation / G protein-coupled acetylcholine receptor signaling pathway ...G protein-coupled receptor activity / adenylate cyclase-activating G protein-coupled receptor signaling pathway / Olfactory Signaling Pathway / Activation of the phototransduction cascade / Prostacyclin signalling through prostacyclin receptor / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / Glucagon signaling in metabolic regulation / G protein-coupled acetylcholine receptor signaling pathway / G-protein activation / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / G beta:gamma signalling through CDC42 / Vasopressin regulates renal water homeostasis via Aquaporins / G beta:gamma signalling through BTK / ADP signalling through P2Y purinoceptor 12 / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / Glucagon-type ligand receptors / Sensory perception of sweet, bitter, and umami (glutamate) taste / photoreceptor disc membrane / G alpha (z) signalling events / Adrenaline,noradrenaline inhibits insulin secretion / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADORA2B mediated anti-inflammatory cytokines production / cellular response to catecholamine stimulus / sensory perception of taste / ADP signalling through P2Y purinoceptor 1 / adenylate cyclase-activating dopamine receptor signaling pathway / G beta:gamma signalling through PI3Kgamma / GPER1 signaling / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / cellular response to prostaglandin E stimulus / Inactivation, recovery and regulation of the phototransduction cascade / G-protein beta-subunit binding / heterotrimeric G-protein complex / G alpha (12/13) signalling events / extracellular vesicle / signaling receptor complex adaptor activity / Thrombin signalling through proteinase activated receptors (PARs) / GTPase binding / retina development in camera-type eye / Ca2+ pathway / phospholipase C-activating G protein-coupled receptor signaling pathway / G alpha (i) signalling events / fibroblast proliferation / G alpha (s) signalling events / G alpha (q) signalling events / Ras protein signal transduction / cell population proliferation / Extra-nuclear estrogen signaling / cell surface receptor signaling pathway / G protein-coupled receptor signaling pathway / apical plasma membrane / lysosomal membrane / GTPase activity / synapse / protein-containing complex binding / signal transduction / extracellular exosome / membrane / plasma membrane / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) / Lama glama (llama) / Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
Authors | Liu ZM / Bu RQ / Xue CY / Guo SC / Yuan DP / Xiao P / Sun JP | |||||||||
Funding support | China, 2 items
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Citation | Journal: Nat.Chem.Biol. / Year: 2022 Title: apo-ADGRG2 coupled to Gs Authors: Liu ZM / Bu RQ / Xue CY / Guo SC / Yuan DP / Xiao P / Sun JP | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_33995.map.gz | 4 MB | EMDB map data format | |
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Header (meta data) | emd-33995-v30.xml emd-33995.xml | 21.1 KB 21.1 KB | Display Display | EMDB header |
Images | emd_33995.png | 19.5 KB | ||
Others | emd_33995_half_map_1.map.gz emd_33995_half_map_2.map.gz | 40.7 MB 40.8 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-33995 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-33995 | HTTPS FTP |
-Validation report
Summary document | emd_33995_validation.pdf.gz | 601.8 KB | Display | EMDB validaton report |
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Full document | emd_33995_full_validation.pdf.gz | 601.3 KB | Display | |
Data in XML | emd_33995_validation.xml.gz | 11.8 KB | Display | |
Data in CIF | emd_33995_validation.cif.gz | 13.7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-33995 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-33995 | HTTPS FTP |
-Related structure data
Related structure data | 7yp7MC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_33995.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | apo-ADGRG2-Gs complex | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.08 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half1 map of apo-ADGRG2-Gs comple
File | emd_33995_half_map_1.map | ||||||||||||
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Annotation | Half1 map of apo-ADGRG2-Gs comple | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half1 map of apo-ADGRG2-Gs comple
File | emd_33995_half_map_2.map | ||||||||||||
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Annotation | Half1 map of apo-ADGRG2-Gs comple | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : apo-ADGRG2-Gs complex
Entire | Name: apo-ADGRG2-Gs complex |
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Components |
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-Supramolecule #1: apo-ADGRG2-Gs complex
Supramolecule | Name: apo-ADGRG2-Gs complex / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
-Supramolecule #2: Guanine nucleotide-binding protein Gs
Supramolecule | Name: Guanine nucleotide-binding protein Gs / type: complex / Chimera: Yes / ID: 2 / Parent: 1 / Macromolecule list: #1-#3 |
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Source (natural) | Organism: Lama glama (llama) |
Recombinant expression | Organism: Escherichia coli (E. coli) |
-Supramolecule #3: Nano-body 35
Supramolecule | Name: Nano-body 35 / type: complex / Chimera: Yes / ID: 3 / Parent: 1 / Macromolecule list: #4 |
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Source (natural) | Organism: Mus musculus (house mouse) |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
-Supramolecule #4: Adhesion G-protein-coupled receptor G2
Supramolecule | Name: Adhesion G-protein-coupled receptor G2 / type: complex / Chimera: Yes / ID: 4 / Parent: 1 / Macromolecule list: #5 |
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-Macromolecule #1: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
Macromolecule | Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 45.683434 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE SGKNTIVKQM RILHVNGFNG EGGEEDPQAA RSNSDGEKA TKVQDIKNNL KEAIETIVAA MSNLVPPVEL ANPENQFRVD YILSVMNVPD FDFPPEFYEH AKALWEDEGV R ACYERSNE ...String: MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE SGKNTIVKQM RILHVNGFNG EGGEEDPQAA RSNSDGEKA TKVQDIKNNL KEAIETIVAA MSNLVPPVEL ANPENQFRVD YILSVMNVPD FDFPPEFYEH AKALWEDEGV R ACYERSNE YQLIDCAQYF LDKIDVIKQA DYVPSDQDLL RCRVLTSGIF ETKFQVDKVN FHMFDVGAQR DERRKWIQCF ND VTAIIFV VASSSYNMVI REDNQTNRLQ AALKLFDSIW NNKWLRDTSV ILFLNKQDLL AEKVLAGKSK IEDYFPEFAR YTT PEDATP EPGEDPRVTR AKYFIRDEFL RISTASGDGR HYCYPHFTCA VDTENIRRVF NDCRDIIQRM HLRQYELL |
-Macromolecule #2: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Macromolecule | Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 39.48916 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MHHHHHHLEV LFQGPGSSQS ELDQLRQEAE QLKNQIRDAR KACADATLSQ ITNNIDPVGR IQMRTRRTLR GHLAKIYAMH WGTDSRLLV SASQDGKLII WDSYTTNKVH AIPLRSSWVM TCAYAPSGNY VACGGLDNIC SIYNLKTREG NVRVSRELAG H TGYLSCCR ...String: MHHHHHHLEV LFQGPGSSQS ELDQLRQEAE QLKNQIRDAR KACADATLSQ ITNNIDPVGR IQMRTRRTLR GHLAKIYAMH WGTDSRLLV SASQDGKLII WDSYTTNKVH AIPLRSSWVM TCAYAPSGNY VACGGLDNIC SIYNLKTREG NVRVSRELAG H TGYLSCCR FLDDNQIVTS SGDTTCALWD IETGQQTTTF TGHTGDVMSL SLAPDTRLFV SGACDASAKL WDVREGMCRQ TF TGHESDI NAICFFPNGN AFATGSDDAT CRLFDLRADQ ELMTYSHDNI ICGITSVSFS KSGRLLLAGY DDFNCNVWDA LKA DRAGVL AGHDNRVSCL GVTDDGMAVA TGSWDSFLKI WN |
-Macromolecule #3: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
Macromolecule | Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 7.861143 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MASNNTASIA QARKLVEQLK MEANIDRIKV SKAAADLMAY CEAHAKEDPL LTPVPASENP FREKKFFCAI L |
-Macromolecule #4: Nano-body 35
Macromolecule | Name: Nano-body 35 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Lama glama (llama) |
Molecular weight | Theoretical: 17.352498 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MKYLLPTAAA GLLLLAAQPA MAQVQLQESG GGLVQPGGSL RLSCAASGFT FSNYKMNWVR QAPGKGLEWV SDISQSGASI SYTGSVKGR FTISRDNAKN TLYLQMNSLK PEDTAVYYCA RCPAPFTRDC FDVTSTTYAY RGQGTQVTVS SHHHHHHEPE A |
-Macromolecule #5: Adhesion G-protein coupled receptor G2
Macromolecule | Name: Adhesion G-protein coupled receptor G2 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 98.934797 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MKTIIALSYI FCLVFADYKD DDDKLKENGN SSLLSPSAES SLVSLIPYSN GTPDAASEVL STLNKTEKSK ITIVKTFNAS GVKSQRNIC NLSSLCNDSV FFRGEIVFQH DEDHNVTQNQ DTANGTFAGV LSLSELKRSE LNKTLQTLSE TYFIVCATAE A QSTVNCTF ...String: MKTIIALSYI FCLVFADYKD DDDKLKENGN SSLLSPSAES SLVSLIPYSN GTPDAASEVL STLNKTEKSK ITIVKTFNAS GVKSQRNIC NLSSLCNDSV FFRGEIVFQH DEDHNVTQNQ DTANGTFAGV LSLSELKRSE LNKTLQTLSE TYFIVCATAE A QSTVNCTF TVKLNETMNV CAMMVTFQTV QIRPMEQCCC SPRTPCPSSP EELEKLQCEL QDPIVCLADQ PHGPPLSSSS KP VVPQATI ISHVASDFSL AEPLDHALMT PSTPSLTQES NLPSPQPTIP LASSPATDLP VQSVVVSSLP QTDLSHTLSP VQS SIPSPT TPAPSVPTEL VTISTPPGET VVNTSTVSDL EAQVSQMEKA LSLGSLEPNL AGEMVNRVSK LLHSPPALLA PLAQ RLLKV VDAIGLQLNF SSTTISLTSP SLALAVIRVN ASNFNTTTFA AQDPTNLQVS LETPPPENSI GAITLPSSLM NNLPA NDVE LASRIQFNFF ETPALFQDPS LENLTLISYV ISSSVTNMTI KNLTRNVTVA LKHINPSPDD LTVKCVFWDL GRNGGK GGW SSDGCSVKDK RMNETICTCS ALASFGILLD LSRTSLPPSQ MMALTFITYI GCGLSSIFLS VTLVTYIAFE KIRRDYP SK ILIQLCAALL LLNLIFLLDS WIALYNTRGF CIAVAVFLHY FLLVSFTWMG LEAFHMYLAL VKVFNTYIRK YILKFCIV G WGIPAVVVSI VLTISPDNYG IGSYGKFPNG TPDDFCWINS NVVFYITVVG YFCVIFLLNV SMFIVVLVQL CRIKKKKQL GAQRKTSIQD LRSIAGLTFL LGITWGFAFF AWGPVNVTFM YLFAIFNTLQ GFFIFIFYCA AKENVRKQWR RYLCCGKLFW FPEKGAILT DTSVKRNDLS IISG |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: EMDB MAP EMDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 92087 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |