- EMDB-32394: Cryo-EM structure of the NR subunit from X. laevis NPC -
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基本情報
登録情報
データベース: EMDB / ID: EMD-32394
タイトル
Cryo-EM structure of the NR subunit from X. laevis NPC
マップデータ
The EM map of NR subunit
試料
複合体: The NR subunit
タンパク質・ペプチド: x 13種
キーワード
nuclear pore complex / nuclear ring / Nup205 / Nup93 / Y complex / Elys / STRUCTURAL PROTEIN
機能・相同性
機能・相同性情報
: / nitrogen compound transport / macromolecule localization / system development / nephron development / GATOR2 complex / macromolecule metabolic process / Seh1-associated complex / nuclear pore inner ring / animal organ development ...: / nitrogen compound transport / macromolecule localization / system development / nephron development / GATOR2 complex / macromolecule metabolic process / Seh1-associated complex / nuclear pore inner ring / animal organ development / COPII-coated vesicle budding / protein exit from endoplasmic reticulum / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore complex assembly / nuclear pore outer ring / protein localization to nuclear inner membrane / nuclear pore organization / COPII vesicle coat / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / attachment of mitotic spindle microtubules to kinetochore / structural constituent of nuclear pore / RNA export from nucleus / nucleocytoplasmic transport / poly(A)+ mRNA export from nucleus / mitotic metaphase chromosome alignment / positive regulation of TOR signaling / mRNA transport / nuclear pore / mRNA export from nucleus / positive regulation of TORC1 signaling / nuclear periphery / cellular response to nutrient levels / cellular response to amino acid starvation / chromosome segregation / kinetochore / protein import into nucleus / protein transport / nuclear membrane / cell division / lysosomal membrane / positive regulation of DNA-templated transcription / structural molecule activity / DNA binding / nucleoplasm / cytoplasm / cytosol 類似検索 - 分子機能
National Natural Science Foundation of China (NSFC)
中国
引用
ジャーナル: Cell Res / 年: 2022 タイトル: Cryo-EM structure of the nuclear ring from Xenopus laevis nuclear pore complex. 著者: Gaoxingyu Huang / Xiechao Zhan / Chao Zeng / Xuechen Zhu / Ke Liang / Yanyu Zhao / Pan Wang / Qifan Wang / Qiang Zhou / Qinghua Tao / Minhao Liu / Jianlin Lei / Chuangye Yan / Yigong Shi / 要旨: Nuclear pore complex (NPC) shuttles cargo across the nuclear envelope. Here we present single-particle cryo-EM structure of the nuclear ring (NR) subunit from Xenopus laevis NPC at an average ...Nuclear pore complex (NPC) shuttles cargo across the nuclear envelope. Here we present single-particle cryo-EM structure of the nuclear ring (NR) subunit from Xenopus laevis NPC at an average resolution of 5.6 Å. The NR subunit comprises two 10-membered Y complexes, each with the nucleoporin ELYS closely associating with Nup160 and Nup37 of the long arm. Unlike the cytoplasmic ring (CR) or inner ring (IR), the NR subunit contains only one molecule each of Nup205 and Nup93. Nup205 binds both arms of the Y complexes and interacts with the stem of inner Y complex from the neighboring subunit. Nup93 connects the stems of inner and outer Y complexes within the same NR subunit, and places its N-terminal extended helix into the axial groove of Nup205 from the neighboring subunit. Together with other structural information, we have generated a composite atomic model of the central ring scaffold that includes the NR, IR, and CR. The IR is connected to the two outer rings mainly through Nup155. This model facilitates functional understanding of vertebrate NPC.