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基本情報
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タイトル | The cryo-EM structure of human pre-C*-I complex | |||||||||
![]() | The cryo-EM map of human pre-C*-I complex | |||||||||
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機能・相同性 | ![]() exon-exon junction subcomplex mago-y14 / negative regulation of selenocysteine incorporation / regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / cellular response to selenite ion / spliceosomal complex disassembly / exon-exon junction complex / selenocysteine insertion sequence binding / negative regulation of proteasomal protein catabolic process / regulation of translation at postsynapse, modulating synaptic transmission / post-mRNA release spliceosomal complex ...exon-exon junction subcomplex mago-y14 / negative regulation of selenocysteine incorporation / regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / cellular response to selenite ion / spliceosomal complex disassembly / exon-exon junction complex / selenocysteine insertion sequence binding / negative regulation of proteasomal protein catabolic process / regulation of translation at postsynapse, modulating synaptic transmission / post-mRNA release spliceosomal complex / regulation of retinoic acid receptor signaling pathway / 3'-5' RNA helicase activity / U2 snRNP binding / intracellular mRNA localization / negative regulation of excitatory postsynaptic potential / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / generation of catalytic spliceosome for first transesterification step / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / regulation of vitamin D receptor signaling pathway / histone pre-mRNA 3'end processing complex / regulation of mRNA processing / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / Deadenylation of mRNA / embryonic brain development / protein methylation / U12-type spliceosomal complex / methylosome / nuclear retinoic acid receptor binding / 7-methylguanosine cap hypermethylation / M-decay: degradation of maternal mRNAs by maternally stored factors / Prp19 complex / positive regulation of androgen receptor activity / poly(A) binding / snRNP binding / U1 snRNP binding / mRNA 3'-end processing / ATP-dependent activity, acting on RNA / embryonic cranial skeleton morphogenesis / pICln-Sm protein complex / RNA splicing, via transesterification reactions / small nuclear ribonucleoprotein complex / pre-mRNA binding / U2-type catalytic step 1 spliceosome / sno(s)RNA-containing ribonucleoprotein complex / C2H2 zinc finger domain binding / regulation of mRNA splicing, via spliceosome / SMN-Sm protein complex / spliceosomal tri-snRNP complex / P granule / telomerase holoenzyme complex / mRNA cis splicing, via spliceosome / positive regulation by host of viral transcription / telomerase RNA binding / U2-type precatalytic spliceosome / U2-type spliceosomal complex / Transport of Mature mRNA derived from an Intron-Containing Transcript / commitment complex / positive regulation of vitamin D receptor signaling pathway / nuclear vitamin D receptor binding / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / Notch binding / positive regulation of mRNA splicing, via spliceosome / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / U4 snRNP / RUNX3 regulates NOTCH signaling / U2 snRNP / RNA Polymerase II Transcription Termination / NOTCH4 Intracellular Domain Regulates Transcription / exploration behavior / U1 snRNP / ubiquitin-ubiquitin ligase activity / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / NOTCH3 Intracellular Domain Regulates Transcription / WD40-repeat domain binding / lipid biosynthetic process / Cajal body / positive regulation of neurogenesis / U2-type prespliceosome / cyclosporin A binding / nuclear androgen receptor binding / regulation of alternative mRNA splicing, via spliceosome / K63-linked polyubiquitin modification-dependent protein binding / positive regulation of G1/S transition of mitotic cell cycle / precatalytic spliceosome / retinoic acid receptor signaling pathway / Notch-HLH transcription pathway / positive regulation of transforming growth factor beta receptor signaling pathway / Formation of paraxial mesoderm / spliceosomal complex assembly / mRNA Splicing - Minor Pathway / SMAD binding / mitotic G2 DNA damage checkpoint signaling / associative learning / protein K63-linked ubiquitination / blastocyst development / protein peptidyl-prolyl isomerization 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.6 Å | |||||||||
![]() | Zhan X / Lu Y / Shi Y | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Mechanism of exon ligation by human spliceosome. 著者: Xiechao Zhan / Yichen Lu / Xiaofeng Zhang / Chuangye Yan / Yigong Shi / ![]() 要旨: Pre-mRNA splicing involves two sequential reactions: branching and exon ligation. The C complex after branching undergoes remodeling to become the C complex, which executes exon ligation. Here, we ...Pre-mRNA splicing involves two sequential reactions: branching and exon ligation. The C complex after branching undergoes remodeling to become the C complex, which executes exon ligation. Here, we report cryo-EM structures of two intermediate human spliceosomal complexes, pre-C-I and pre-C-II, both at 3.6 Å. In both structures, the 3' splice site is already docked into the active site, the ensuing 3' exon sequences are anchored on PRP8, and the step II factor FAM192A contacts the duplex between U2 snRNA and the branch site. In the transition of pre-C-I to pre-C-II, the step II factors Cactin, FAM32A, PRKRIP1, and SLU7 are recruited. Notably, the RNA helicase PRP22 is positioned quite differently in the pre-C-I, pre-C-II, and C complexes, suggesting a role in 3' exon binding and proofreading. Together with information on human C and C complexes, our studies recapitulate a molecular choreography of the C-to-C transition, revealing mechanistic insights into exon ligation. | |||||||||
履歴 |
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構造の表示
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ダウンロードとリンク
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マップデータ | ![]() | 230.2 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 59 KB 59 KB | 表示 表示 | ![]() |
画像 | ![]() | 62.7 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7w59MC ![]() 7w5aC ![]() 7w5bC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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注釈 | The cryo-EM map of human pre-C*-I complex | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.338 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : Human pre-C*-I complex
+超分子 #1: Human pre-C*-I complex
+分子 #1: Pre-mRNA-processing-splicing factor 8
+分子 #2: 116 kDa U5 small nuclear ribonucleoprotein component
+分子 #3: U5 small nuclear ribonucleoprotein 40 kDa protein
+分子 #6: Crooked neck-like protein 1
+分子 #7: Cell division cycle 5-like protein
+分子 #8: Pre-mRNA-splicing factor SYF2
+分子 #9: Protein BUD31 homolog
+分子 #10: Pre-mRNA-splicing factor RBM22
+分子 #11: Spliceosome-associated protein CWC15 homolog
+分子 #12: SNW domain-containing protein 1
+分子 #13: Peptidyl-prolyl cis-trans isomerase-like 1
+分子 #14: Pleiotropic regulator 1
+分子 #15: Serine/arginine repetitive matrix protein 2
+分子 #16: Pre-mRNA-splicing factor CWC22 homolog
+分子 #17: Pre-mRNA-processing factor 17
+分子 #21: Small nuclear ribonucleoprotein-associated proteins B and B'
+分子 #22: PSME3-interacting protein
+分子 #23: Pre-mRNA-splicing factor SYF1
+分子 #24: Peptidyl-prolyl cis-trans isomerase E
+分子 #25: ATP-dependent RNA helicase DHX8
+分子 #26: Small nuclear ribonucleoprotein Sm D3
+分子 #27: Small nuclear ribonucleoprotein Sm D1
+分子 #28: Small nuclear ribonucleoprotein Sm D2
+分子 #29: Small nuclear ribonucleoprotein F
+分子 #30: Small nuclear ribonucleoprotein E
+分子 #31: Small nuclear ribonucleoprotein G
+分子 #32: Protein mago nashi homolog
+分子 #33: RNA-binding protein 8A
+分子 #34: Eukaryotic initiation factor 4A-III
+分子 #35: Protein CASC3
+分子 #36: RNA helicase aquarius
+分子 #37: U2 small nuclear ribonucleoprotein A'
+分子 #38: U2 small nuclear ribonucleoprotein B''
+分子 #39: Pre-mRNA-processing factor 19
+分子 #40: Pre-mRNA-splicing factor SPF27
+分子 #4: pre-mRNA
+分子 #5: pre-mRNA
+分子 #18: U5 snRNA
+分子 #19: U6 snRNA
+分子 #20: U2 snRNA
+分子 #41: INOSITOL HEXAKISPHOSPHATE
+分子 #42: GUANOSINE-5'-TRIPHOSPHATE
+分子 #43: MAGNESIUM ION
+分子 #44: ZINC ION
+分子 #45: ADENOSINE-5'-TRIPHOSPHATE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.9 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: SUPER-RESOLUTION / 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 1.2 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.6 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 192274 |
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初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |