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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Cryo-EM structure of the ATP-binding cassette sub-family D member 1 from Homo sapiens | |||||||||
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![]() | Membrane protein / LIPID TRANSPORT / TRANSLOCASE | |||||||||
機能・相同性 | ![]() ABC-type fatty-acyl-CoA transporter activity / peroxisomal membrane transport / very long-chain fatty-acyl-CoA catabolic process / very long-chain fatty acyl-CoA hydrolase activity / positive regulation of unsaturated fatty acid biosynthetic process / Linoleic acid (LA) metabolism / Defective ABCD1 causes ALD / alpha-linolenic acid metabolic process / long-chain fatty acid catabolic process / long-chain fatty acid import into peroxisome ...ABC-type fatty-acyl-CoA transporter activity / peroxisomal membrane transport / very long-chain fatty-acyl-CoA catabolic process / very long-chain fatty acyl-CoA hydrolase activity / positive regulation of unsaturated fatty acid biosynthetic process / Linoleic acid (LA) metabolism / Defective ABCD1 causes ALD / alpha-linolenic acid metabolic process / long-chain fatty acid catabolic process / long-chain fatty acid import into peroxisome / very long-chain fatty acid catabolic process / alpha-linolenic acid (ALA) metabolism / regulation of fatty acid beta-oxidation / Beta-oxidation of very long chain fatty acids / Class I peroxisomal membrane protein import / very long-chain fatty acid metabolic process / sterol homeostasis / peroxisome organization / regulation of mitochondrial depolarization / ABC transporters in lipid homeostasis / myelin maintenance / fatty acyl-CoA hydrolase activity / regulation of cellular response to oxidative stress / 加水分解酵素; エステル加水分解酵素; 3価のアルコールのエステル加水分解酵素 / positive regulation of fatty acid beta-oxidation / linoleic acid metabolic process / regulation of oxidative phosphorylation / トランスロカーゼ; 他の化合物の輸送を触媒; ヌクレオシド三リン酸の加水分解に伴う / fatty acid elongation / peroxisomal membrane / long-chain fatty acid transmembrane transporter activity / fatty acid beta-oxidation / ATPase-coupled transmembrane transporter activity / negative regulation of cytokine production involved in inflammatory response / fatty acid homeostasis / negative regulation of reactive oxygen species biosynthetic process / neuron projection maintenance / mitochondrial membrane / ADP binding / peroxisome / protein heterodimerization activity / lysosomal membrane / endoplasmic reticulum membrane / perinuclear region of cytoplasm / enzyme binding / protein homodimerization activity / ATP hydrolysis activity / ATP binding / identical protein binding / membrane / cytoplasm / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.4 Å | |||||||||
![]() | Yang GH / Jia YT / Zhang YM | |||||||||
資金援助 | 1件
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![]() | ![]() タイトル: Structural and functional insights of the human peroxisomal ABC transporter ALDP. 著者: Yutian Jia / Yanming Zhang / Wenhao Wang / Jianlin Lei / Zhengxin Ying / Guanghui Yang / ![]() 要旨: Adrenoleukodystrophy protein (ALDP) is responsible for the transport of very-long-chain fatty acids (VLCFAs) and corresponding CoA-esters across the peroxisomal membrane. Dysfunction of ALDP leads to ...Adrenoleukodystrophy protein (ALDP) is responsible for the transport of very-long-chain fatty acids (VLCFAs) and corresponding CoA-esters across the peroxisomal membrane. Dysfunction of ALDP leads to peroxisomal metabolic disorder exemplified by X-linked adrenoleukodystrophy (ALD). Hundreds of ALD-causing mutations have been identified on ALDP. However, the pathogenic mechanisms of these mutations are restricted to clinical description due to limited structural and biochemical characterization. Here we report the cryo-electron microscopy structure of human ALDP with nominal resolution at 3.4 Å. ALDP exhibits a cytosolic-facing conformation. Compared to other lipid ATP-binding cassette transporters, ALDP has two substrate binding cavities formed by the transmembrane domains. Such structural organization may be suitable for the coordination of VLCFAs. Based on the structure, we performed integrative analysis of the cellular trafficking, protein thermostability, ATP hydrolysis, and the transport activity of representative mutations. These results provide a framework for understanding the working mechanism of ALDP and pathogenic roles of disease-associated mutations. | |||||||||
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マップデータ | ![]() | 28.2 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 9.4 KB 9.4 KB | 表示 表示 | ![]() |
画像 | ![]() | 54.1 KB | ||
Filedesc metadata | ![]() | 5.3 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 498.8 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 498.3 KB | 表示 | |
XML形式データ | ![]() | 5.6 KB | 表示 | |
CIF形式データ | ![]() | 6.4 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7vr1MC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.0979 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
-全体 : Human ATP-binding cassette sub-family D member 1
全体 | 名称: Human ATP-binding cassette sub-family D member 1 |
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要素 |
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-超分子 #1: Human ATP-binding cassette sub-family D member 1
超分子 | 名称: Human ATP-binding cassette sub-family D member 1 / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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由来(天然) | 生物種: ![]() |
-分子 #1: ATP-binding cassette sub-family D member 1
分子 | 名称: ATP-binding cassette sub-family D member 1 / タイプ: protein_or_peptide / ID: 1 / コピー数: 2 / 光学異性体: LEVO / EC番号: ec: 7.6.2.4 |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 83.040867 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: MPVLSRPRPW RGNTLKRTAV LLALAAYGAH KVYPLVRQCL APARGLQAPA GEPTQEASGV AAAKAGMNRV FLQRLLWLLR LLFPRVLCR ETGLLALHSA ALVSRTFLSV YVARLDGRLA RCIVRKDPRA FGWQLLQWLL IALPATFVNS AIRYLEGQLA L SFRSRLVA ...文字列: MPVLSRPRPW RGNTLKRTAV LLALAAYGAH KVYPLVRQCL APARGLQAPA GEPTQEASGV AAAKAGMNRV FLQRLLWLLR LLFPRVLCR ETGLLALHSA ALVSRTFLSV YVARLDGRLA RCIVRKDPRA FGWQLLQWLL IALPATFVNS AIRYLEGQLA L SFRSRLVA HAYRLYFSQQ TYYRVSNMDG RLRNPDQSLT EDVVAFAASV AHLYSNLTKP LLDVAVTSYT LLRAARSRGA GT AWPSAIA GLVVFLTANV LRAFSPKFGE LVAEEARRKG ELRYMHSRVV ANSEEIAFYG GHEVELALLQ RSYQDLASQI NLI LLERLW YVMLEQFLMK YVWSASGLLM VAVPIITATG YSESDAEAVK KAALEKKEEE LVSERTEAFT IARNLLTAAA DAIE RIMSS YKEVTELAGY TARVHEMFQV FEDVQRCHFK RPRELEDAQA GSGTIGRSGV RVEGPLKIRG QVVDVEQGII CENIP IVTP SGEVVVASLN IRVEEGMHLL ITGPNGCGKS SLFRILGGLW PTYGGVLYKP PPQRMFYIPQ RPYMSVGSLR DQVIYP DSV EDMQRKGYSE QDLEAILDVV HLHHILQREG GWEAMCDWKD VLSGGEKQRI GMARMFYHRP KYALLDECTS AVSIDVE GK IFQAAKDAGI ALLSITHRPS LWKYHTHLLQ FDGEGGWKFE KLDSAARLSL TEEKQRLEQQ LAGIPKMQRR LQELCQIL G EAVAPAHVPA PSPQGPGGLQ GAST UniProtKB: ATP-binding cassette sub-family D member 1 |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 1.5625 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 1.8 µm / 最小 デフォーカス(公称値): 1.5 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
初期モデル | モデルのタイプ: NONE |
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最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.4 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 472168 |
初期 角度割当 | タイプ: PROJECTION MATCHING |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |