+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-31671 | |||||||||
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Title | map7, one conformation of the IGF1R/insulin complex | |||||||||
Map data | map7, one conformation of the IGF1R/insulin complex | |||||||||
Sample |
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Function / homology | Function and homology information cardiac atrium development / negative regulation of cholangiocyte apoptotic process / insulin-like growth factor receptor activity / positive regulation of steroid hormone biosynthetic process / protein kinase complex / Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) / IRS-related events triggered by IGF1R / insulin-like growth factor binding / protein transporter activity / negative regulation of muscle cell apoptotic process ...cardiac atrium development / negative regulation of cholangiocyte apoptotic process / insulin-like growth factor receptor activity / positive regulation of steroid hormone biosynthetic process / protein kinase complex / Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) / IRS-related events triggered by IGF1R / insulin-like growth factor binding / protein transporter activity / negative regulation of muscle cell apoptotic process / cellular response to progesterone stimulus / positive regulation of DNA metabolic process / cellular response to zinc ion starvation / cellular response to aldosterone / insulin receptor complex / cellular response to testosterone stimulus / negative regulation of hepatocyte apoptotic process / insulin-like growth factor I binding / insulin receptor activity / transcytosis / alphav-beta3 integrin-IGF-1-IGF1R complex / response to alkaloid / Respiratory syncytial virus (RSV) attachment and entry / regulation of JNK cascade / cellular response to angiotensin / positive regulation of protein-containing complex disassembly / dendritic spine maintenance / cellular response to insulin-like growth factor stimulus / insulin binding / response to L-glutamate / negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / negative regulation of MAPK cascade / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / establishment of cell polarity / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / Insulin processing / positive regulation of axon regeneration / regulation of protein secretion / amyloid-beta clearance / positive regulation of osteoblast proliferation / positive regulation of peptide hormone secretion / positive regulation of respiratory burst / positive regulation of cytokinesis / peptidyl-tyrosine autophosphorylation / negative regulation of acute inflammatory response / Regulation of gene expression in beta cells / alpha-beta T cell activation / regulation of cellular amino acid metabolic process / negative regulation of respiratory burst involved in inflammatory response / insulin receptor substrate binding / estrous cycle / positive regulation of dendritic spine maintenance / positive regulation of glycogen biosynthetic process / G-protein alpha-subunit binding / response to vitamin E / Synthesis, secretion, and deacylation of Ghrelin / negative regulation of protein secretion / regulation of protein localization to plasma membrane / SHC-related events triggered by IGF1R / fatty acid homeostasis / Signal attenuation / negative regulation of lipid catabolic process / negative regulation of gluconeogenesis / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / cellular response to dexamethasone stimulus / phosphatidylinositol 3-kinase binding / COPI-mediated anterograde transport / positive regulation of lipid biosynthetic process / negative regulation of reactive oxygen species biosynthetic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / positive regulation of insulin receptor signaling pathway / nitric oxide-cGMP-mediated signaling / transport vesicle / positive regulation of protein autophosphorylation / cellular response to transforming growth factor beta stimulus / Insulin receptor recycling / T-tubule / insulin-like growth factor receptor binding / cellular response to estradiol stimulus / neuron projection maintenance / NPAS4 regulates expression of target genes / positive regulation of protein metabolic process / positive regulation of brown fat cell differentiation / phosphatidylinositol 3-kinase/protein kinase B signal transduction / activation of protein kinase B activity / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of glycolytic process / positive regulation of mitotic nuclear division / Insulin receptor signalling cascade / positive regulation of smooth muscle cell proliferation / cerebellum development / axonogenesis / insulin-like growth factor receptor signaling pathway / positive regulation of nitric-oxide synthase activity / positive regulation of cytokine production / positive regulation of long-term synaptic potentiation Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.6 Å | |||||||||
Authors | Liu C / Zhang J / Zhang X / Wei T / Wu C | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: map7, one conformation of the IGF1R/insulin complex Authors: Liu C / Zhang J / Zhang X / Wei T / Wu C | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_31671.map.gz | 7.9 MB | EMDB map data format | |
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Header (meta data) | emd-31671-v30.xml emd-31671.xml | 7.3 KB 7.3 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_31671_fsc.xml | 11.4 KB | Display | FSC data file |
Images | emd_31671.png | 115.8 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-31671 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-31671 | HTTPS FTP |
-Related structure data
Related structure data | 7v3pM M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_31671.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | map7, one conformation of the IGF1R/insulin complex | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.08 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : Complex of the dimer IGF1R with insulin
Entire | Name: Complex of the dimer IGF1R with insulin |
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Components |
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-Supramolecule #1: Complex of the dimer IGF1R with insulin
Supramolecule | Name: Complex of the dimer IGF1R with insulin / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 1.5 mg/mL |
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Buffer | pH: 7.4 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |