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データを開く
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基本情報
登録情報 | データベース: EMDB / ID: EMD-30158 | ||||||||||||||||||
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タイトル | Epstein-Barr virus, C1 portal-proximal penton vertex, CATC binding | ||||||||||||||||||
![]() | For the entry D_1300016325, the EM map (C1) authors have provided shows a pseudo-C5 symmetry with four "ASU" copies are identity to that provided in other related entry D_1300016276. The coordinates authors uploaded only represents the unique one "ASU" due to the limitation of the chain numbers (beyond 62 chains if the model could overlap with the whole map). | ||||||||||||||||||
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![]() | portal-proximal penton vertex / CATC binding / tegumented capsid / asymmetric reconstruction / VIRAL PROTEIN | ||||||||||||||||||
機能・相同性 | ![]() T=16 icosahedral viral capsid / viral genome packaging / viral tegument / viral capsid assembly / chromosome organization / viral process / viral penetration into host nucleus / viral capsid / host cell / symbiont-mediated perturbation of host ubiquitin-like protein modification ...T=16 icosahedral viral capsid / viral genome packaging / viral tegument / viral capsid assembly / chromosome organization / viral process / viral penetration into host nucleus / viral capsid / host cell / symbiont-mediated perturbation of host ubiquitin-like protein modification / ubiquitinyl hydrolase 1 / host cell cytoplasm / cysteine-type deubiquitinase activity / 加水分解酵素; プロテアーゼ; ペプチド結合加水分解酵素; システインプロテアーゼ / symbiont entry into host cell / host cell nucleus / structural molecule activity / proteolysis / DNA binding 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | ![]() ![]() | ||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.3 Å | ||||||||||||||||||
![]() | Li Z / Yu X / Zeng M | ||||||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: CryoEM structure of the tegumented capsid of Epstein-Barr virus. 著者: Zhihai Li / Xiao Zhang / Lili Dong / Jingjing Pang / Miao Xu / Qian Zhong / Mu-Sheng Zeng / Xuekui Yu / ![]() 要旨: Epstein-Barr virus (EBV) is the primary cause of infectious mononucleosis and has been shown to be closely associated with various malignancies. Here, we present a complete atomic model of EBV, ...Epstein-Barr virus (EBV) is the primary cause of infectious mononucleosis and has been shown to be closely associated with various malignancies. Here, we present a complete atomic model of EBV, including the icosahedral capsid, the dodecameric portal and the capsid-associated tegument complex (CATC). Our in situ portal from the tegumented capsid adopts a closed conformation with its channel valve holding the terminal viral DNA and with its crown region firmly engaged by three layers of ring-like dsDNA, which, together with the penton flexibility, effectively alleviates the capsid inner pressure placed on the portal cap. In contrast, the CATCs, through binding to the flexible penton vertices in a stoichiometric manner, accurately increase the inner capsid pressure to facilitate the pressure-driven genome delivery. Together, our results provide important insights into the mechanism by which the EBV capsid, portal, packaged genome and the CATCs coordinately achieve a pressure balance to simultaneously benefit both viral genome retention and ejection. | ||||||||||||||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 93.7 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 22.1 KB 22.1 KB | 表示 表示 | ![]() |
画像 | ![]() | 168.4 KB | ||
Filedesc metadata | ![]() | 9.5 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | For the entry D_1300016325, the EM map (C1) authors have provided shows a pseudo-C5 symmetry with four "ASU" copies are identity to that provided in other related entry D_1300016276. The coordinates authors uploaded only represents the unique one "ASU" due to the limitation of the chain numbers (beyond 62 chains if the model could overlap with the whole map). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.31 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
-全体 : Human gammaherpesvirus 4
全体 | 名称: ![]() |
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要素 |
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-超分子 #1: Human gammaherpesvirus 4
超分子 | 名称: Human gammaherpesvirus 4 / タイプ: virus / ID: 1 / 親要素: 0 / NCBI-ID: 10376 / 生物種: Human gammaherpesvirus 4 / ウイルスタイプ: VIRION / ウイルス・単離状態: STRAIN / ウイルス・エンベロープ: Yes / ウイルス・中空状態: No |
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-分子 #1: Capsid vertex component 2
分子 | 名称: Capsid vertex component 2 / タイプ: protein_or_peptide / ID: 1 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() 株: B95-8 |
分子量 | 理論値: 62.525469 KDa |
配列 | 文字列: MALSGHVLID PARLPRDTGP ELMWAPSLRN SLRVSPEALE LAEREAERAR SERWDRCAQV LKNRLLRVEL DGIMRDHLAR AEEIRQDLD AVVAFSDGLE SMQVRSPSTG GRSAPAPPSP SPAQPFTRLT GNAQYAVSIS PTDPPLMVAG SLAQTLLGNL Y GNINQWVP ...文字列: MALSGHVLID PARLPRDTGP ELMWAPSLRN SLRVSPEALE LAEREAERAR SERWDRCAQV LKNRLLRVEL DGIMRDHLAR AEEIRQDLD AVVAFSDGLE SMQVRSPSTG GRSAPAPPSP SPAQPFTRLT GNAQYAVSIS PTDPPLMVAG SLAQTLLGNL Y GNINQWVP SFGPWYRTMS ANAMQRRVFP KQLRGNLNFT NSVSLKLMTE VVAVLEGTTQ DFFSDVRHLP DLQAALILSV AY LLLQGGS SHQQRPLPAS REELLELGPE SLEKIIADLK AKSPGGNFMI LTSGNKEARQ SIAPLNRQAA YPPGTFADNK IYN LFVGAG LLPTTAALNV PGAAGRDRDL VYRIANQIFG EDVPPFSSHQ WNLRVGLAAL EALMLVYTLC ETANLAEAAT RRLH LSSLL PQAMQRRKPA MASAGMPGAY PVQTLFRHGE LFRFIWAHYV RPTVAADPQA SISSLFPGLV LLALELKLMD GQAPS HYAI NLTGQKFDTL FEIINQKLLF HDPAAMLAAR TQLRLAFEDG VGVALGRPSP MLAAREILER QFSASDDYDR LYFLTL GYL ASPVAPS UniProtKB: Capsid vertex component 2 |
-分子 #2: Capsid vertex component 1
分子 | 名称: Capsid vertex component 1 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() 株: B95-8 |
分子量 | 理論値: 54.527941 KDa |
配列 | 文字列: MDVHIDNQVL SGLGTPLLVH LFVPDTVMAE LCPNRVPNCE GAWCQTLFSD RTGLTRVCRV FAARGMLPGR PSHRGTFTSV PVYCDEGLP ELYNPFHVAA LRFYDEGGLV GELQIYYLSL FEGAKRALTD GHLIREASGV QESAAAMQPI PIDPGPPGGA G IEHMPVAA ...文字列: MDVHIDNQVL SGLGTPLLVH LFVPDTVMAE LCPNRVPNCE GAWCQTLFSD RTGLTRVCRV FAARGMLPGR PSHRGTFTSV PVYCDEGLP ELYNPFHVAA LRFYDEGGLV GELQIYYLSL FEGAKRALTD GHLIREASGV QESAAAMQPI PIDPGPPGGA G IEHMPVAA AQVEHPKTYD LKQILLEITQ EENRGEQRLG HAGSPALCLG LRLRAGAETK AAAETSVSKH HPALENPSNI RG SAGGEGG GGRAGTGGTV GVGSGALSRV PVSFSKTRRA IRESRALVRG IAHIFSPHAL YVVTYPELSA QGRLHRMTAV THA SPATDL AEVSILGAPE REFRFLISVA LRISASFREK LAMQAWTAQQ EIPVVIPTSY SRIYKNSDLI REAFFTVQTR VSWE SCWVK ATISNAPKTP DACLWIDSHP LYEEGASAWG KVIDSRPPGG LVGAASQLVA LGTDGHCVHL ATTSDGQAFL VLPGG FVIK GQLALTPEER GYILARHGIR REQ UniProtKB: Capsid vertex component 1 |
-分子 #3: Large tegument protein deneddylase
分子 | 名称: Large tegument protein deneddylase / タイプ: protein_or_peptide / ID: 3 / コピー数: 2 / 光学異性体: LEVO / EC番号: ubiquitinyl hydrolase 1 |
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由来(天然) | 生物種: ![]() 株: B95-8 |
分子量 | 理論値: 338.310625 KDa |
配列 | 文字列: MSNGDWGQSQ RTRGTGPVRG IRTMDVNAPG GGSGGSALRI LGTASCNQAH CKFGRFAGIQ CVSNCVLYLV KSFLAGRPLT SRPELDEVL DEGARLDALM RQSGILKGHE MAQLTDVPSS VVLRGGGRVH IYRSAEIFGL VLFPAQIANS AVVQSLAEVL H GSYNGVAQ ...文字列: MSNGDWGQSQ RTRGTGPVRG IRTMDVNAPG GGSGGSALRI LGTASCNQAH CKFGRFAGIQ CVSNCVLYLV KSFLAGRPLT SRPELDEVL DEGARLDALM RQSGILKGHE MAQLTDVPSS VVLRGGGRVH IYRSAEIFGL VLFPAQIANS AVVQSLAEVL H GSYNGVAQ FILYICDIYA GAIIIETDGS FYLFDPHCQK DAAPGTPAHV RVSTYAHDIL QYVGAPGAQY TCVHLYFLPE AF ETEDPRI FMLEHYGVYD FYEANGSGFD LVGPELVSSD GEAAGTPGAD SSPPVMLPFE RRIIPYNLRP LPSRSFTSDS FPA ARYSPA KTNSPPSSPA SAAPASAAPA SAAPASAAPA SAAPASAAPA SAAPASAAPA SSPPLFIPIP GLGHTPGVPA PSTP PRASS GAAPQTPKRK KGLGKDSPHK KPTSGRRLPL SSTTDTEDDQ LPRTHVPPHR PPSAARLPPP VIPIPHQSPP ASPTP HPAP VSTIAPSVTP SPRLPLQIPI PLPQAAPSNP KIPLTTPSPS PTAAAAPTTT TLSPPPTQQQ PPQSAAPAPS PLLPQQ QPT PSAAPAPSPL LPQQQPPPSA ARAPSPLPPQ QQPLPSATPA PPPAQQLPPS ATTLEPEKNH PPAADRAGTE ISPSPPF GQ QPSFGDDASG GSGLVRYLSD LEEPFLSMSD SEEAESDLAS DIPTTEDEDM FEDEVFSNSL ESGSSAPTSP ITLDTARS Q YYQTTFDIET PEMDFVPLES NIARIAGHTY QEQAIVYDPA SNREVPEADA LSMIDYLLVT VVLEQGLIRS RDRSSVLNL LEFLKDWSGH LQVPTLDLEQ LLTSELNIQN LANMLSENKG RAGEFHKHLA AKLEACLPSL ATKDAVRVDA GAKMLAEIPQ LAESDDGKF DLEAARRRLT DLLSGGDQEA GEGGGEPEDN SIYRGPHVDV PLVLDDESWK RLLSLAEAAR TAVARQQAGV D EEDVRFLA LLTAIEYGAP PAASVPPFVH NVAVRSKNAA LHVRRCTADI RDKVASAASD YLSYLEDPSL PTVMDFDDLL TH LRHTCQI IASLPLLNIR YTSIEWDYRE LLYLGTALSD MSGIPWPLER VEEDDPSIAP LPEFETVAKK QKELETTREN EKR LRTILD DIEAMLGLAG VASAPGAPIS PASPSATPAN HDNPEATPPL ADTAALTIPV IEKYIANAGS IVGAAKNPTY IRLR DTIQQ IVRSKKYLMN ILKSITFYTI DNYIASFEES IDHLYRDLPV LDPEVQDGID RILDPMVSEA LHTFEMGNRL TLEPA RLVA LQNFATHSTL KETAAAVNLL PGLLAVYDAT ITGQAPEDAL RLLSGLQNQL SQTLIPGKLK KRFLSYLQKL KNNNND QLR QKEVQAWRLE AEGFKPATEE QLEAFLDTAP NKELKRQYEK KLRQLMETGR KEKEKLREQE DKERQERRAR EANEAWA RI RKALGARPEP APTSPDDWNT LLASLLPDNT DSAAAAAAAV ARNTDILDSL TQILAAMLLG ITRVRRERLR SLLVDDGG A AERMEAAEPG WFTDIETGPL ARLDAWPATP AATAKEGGGG RGAEEAAGAL FRARTAADAI RSALAQTRQA LQSPDMKSA VVNTDLEAPY AEYERGLAGL LEKRRAAEAA LTAIVSEYVD RTLPEATNDP GQANLPPPPT IPQATAPPRL ASDSALWPKK PQLLTRRER DDLLQATGDF FSELLTEAEA AEVRALEEQV RESQTLMAKA HEMAASTRRG FHTALEAVLS RSRDEAPDDE L RSLLPSPP KAPVQAPLEA ALARAAAGNG SWPYRKSLAA AKWIRGICEA VRGLSEGALA LAGGAGAWLN LAAAADGEIH EL TRLLEVE GMAQNSMDGM EELRLALATL DPKRVAGGKE TVADWKRRLS RLEAIIQEAQ EESQLQGTLQ DLVTQARGHT DPR QLKIVV EAARGLALGA SAGSQYALLK DKLLRYASAK QSFLAFYETA QPTVFVKHPL TNNLPLLITI SAPPTGWGNG APTR RAQFL AAAGPAKYAG TLWLETESPC DPLNPAYVSA DTQEPLNYIP VYHNFLEYVM PTVLENPEAF SLTPAGRPQA IGPPQ DDQE RRRRTLASVA SARLSAAAAD SYWDTWPDVE SNAGELLREY VSAPKALMED LADNPIVAMT LLAHASLIAS RNHPPY PAP ATDREVILLE QREMMALLVG THPAYAAAFL GAPSFYAGLG LVSALARDGG LGDLLSDSVL TYRLVRSPAS GRGGMPS TT RGSNDGEDAR RLTRHRIAGP PTGFIFFQDA WEEMDTRAAL WPHPEFLGLV HNQSTARARA CMLLLARRCF APEALQQL W HSLRPLEGPV AFQDYLRDFV KQAYTRGEEL PRAEGLEVPR ETPSSYGTVT GRALRNLMPY GTPITGPKRG SGDTIPVSV FEAAVAAAFL GRPLTLFVSS QYLFNLKTLG QVRVVAPLLY CDGHSEPFRS LVETISLNFL QDLDGYSESF EPEMSIFARQ AVWLRELLT EARAAKPKEA RPPTVAILAN RKNIIWKCFT YRHNLPDVQF YFNAAGASRW PTDVLNPSFY EHEDPPLPVG Y QLPPNPRN VQELFSGFPP RVGHGLVSGD GFQSADNTPA SSDRLQQLGG GETDQGEKGS TTAESEASGP PSPQSPLLEK VA PGRPRDW LSPTSSPRDV TVTPGLAAPI TLPGPRLMAR PYFGAETRAS ESPDRSPGSS PRPWPKDSLE LLPQPAPQQP PSS PWASEQ GPIVYTLSPH STPSTASGSQ KKHTIQIPGL VPSQKPSYPP SAPYKPGQST GGIAPTPSAA SLTTFGLQPQ DTQA SSQDP PYGHSIMQRE KKQQGGREEA AEIRPSATRL PTAVGLRPRA PVVAAGAAAS ATPAFDPGEA PSGFPIPQAP ALGSG LAAP AHTPVGALAP RPQKTQAQRP QDAAALPTPT IKAVGARPVP KATGALAAGA RPRGQPTAAP PSAASPPRVS LPVRSR QQQ SPAIPLPPMH SGSEPGARPE VRLSQYRHAG PQTYTVRKEA PPSAASQLPK MPKCKDSMYY PPSGSARYPA PFQALSF SQ SVASPAPSSD QTTLLWNTPS VVTQFLSIED IIREVVTGGS TSGDLVVPSG SPSSLSTAAP EQDLRYSLTL SQASRVLS R FVSQLRRKLE RSTHRLIADL ERLKFLYL UniProtKB: Large tegument protein deneddylase |
-分子 #4: Small capsomere-interacting protein
分子 | 名称: Small capsomere-interacting protein / タイプ: protein_or_peptide / ID: 4 / コピー数: 5 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() 株: B95-8 |
分子量 | 理論値: 18.1691 KDa |
配列 | 文字列: MARRLPKPTL QGRLEADFPD SPLLPKFQEL NQNNLPNDVF REAQRSYLVF LTSQFCYEEY VQRTFGVPRR QRAIDKRQRA SVAGAGAHA HLGGSSATPV QQAQAAASAG TGALASSAPS TAVAQSATPS VSSSISSLRA ATSGATAAAS AAAAVDTGSG G GGQPHDTA PRGARKKQ UniProtKB: Small capsomere-interacting protein |
-分子 #5: Major capsid protein
分子 | 名称: Major capsid protein / タイプ: protein_or_peptide / ID: 5 / コピー数: 5 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() 株: B95-8 |
分子量 | 理論値: 154.086828 KDa |
配列 | 文字列: MASNEGVENR PFPYLTVDAD LLSNLRQSAA EGLFHSFDLL VGKDAREAGI KFEVLLGVYT NAIQYVRFLE TALAVSCVNT EFKDLSRMT DGKIQFRISV PTIAHGDGRR PSKQRTFIVV KNCHKHHIST EMELSMLDLE ILHSIPETPV EYAEYVGAVK T VASALQFG ...文字列: MASNEGVENR PFPYLTVDAD LLSNLRQSAA EGLFHSFDLL VGKDAREAGI KFEVLLGVYT NAIQYVRFLE TALAVSCVNT EFKDLSRMT DGKIQFRISV PTIAHGDGRR PSKQRTFIVV KNCHKHHIST EMELSMLDLE ILHSIPETPV EYAEYVGAVK T VASALQFG VDALERGLIN TVLSVKLRHA PPMFILQTLA DPTFTERGFS KTVKSDLIAM FKRHLLEHSF FLDRAENMGS GF SQYVRSR LSEMVAAVSG ESVLKGVSTY TTAKGGEPVG GVFIVTDNVL RQLLTFLGEE ADNQIMGPSS YASFVVRGEN LVT AVSYGR VMRTFEHFMA RIVDSPEKAG STKSDLPAVA AGVEDQPRVP ISAAVIKLGN HAVAVESLQK MYNDTQSPYP LNRR MQYSY YFPVGLFMPN PKYTTSAAIK MLDNPTQQLP VEAWIVNKNN LLLAFNLQNA LKVLCHPRLH TPAHTLNSLN AAPAP RDRR ETYSLQHRRP NHMNVLVIVD EFYDNKYAAP VTDIALKCGL PTEDFLHPSN YDLLRLELHP LYDIYIGRDA GERARH RAV HRLMVGNLPT PLAPAAFQEA RGQQFETATS LAHVVDQAVI ETVQDTAYDT AYPAFFYVVE AMIHGFEEKF VMNVPLV SL CINTYWERSG RLAFVNSFSM IKFICRHLGN NAISKEAYSM YRKIYGELIA LEQALMRLAG SDVVGDESVG QYVCALLD P NLLPPVAYTD IFTHLLTVSD RAPQIIIGNE VYADTLAAPQ FIERVGNMDE MAAQFVALYG YRVNGDHDHD FRLHLGPYV DEGHADVLEK IFYYVFLPTC TNAHMCGLGV DFQHVAQTLA YNGPAFSHHF TRDEDILDNL ENGTLRDLLE ISDLRPTVGM IRDLSASFM TCPTFTRAVR VSVDNDVTQQ LAPNPADKRT EQTVLVNGLV AFAFSERTRA VTQCLFHAIP FHMFYGDPRV A ATMHQDVA TFVMRNPQQR AVEAFNRPEQ LFAEYREWHR SPMGKYAAEC LPSLVSISGM TAMHIKMSPM AYIAQAKLKI HP GVAMTVV RTDEILSENI LFSSRASTSM FIGTPNVSRR EARVDAVTFE VHHEMASIDT GLSYSSTMTP ARVAAITTDM GIH TQDFFS VFPAEAFGNQ QVNDYIKAKV GAQRNGTLLR DPRTYLAGMT NVNGAPGLCH GQQATCEIIV TPVTADVAYF QKSN SPRGR AACVVSCENY NQEVAEGLIY DHSRPDAAYE YRSTVNPWAS QLGSLGDIMY NSSYRQTAVP GLYSPCRAFF NKEEL LRNN RGLYNMVNEY SQRLGGHPAT SNTEVQFVVI AGTDVFLEQP CSFLQEAFPA LSASSRALID EFMSVKQTHA PIHYGH YII EEVAPVRRIL KFGNKVVF UniProtKB: Major capsid protein |
-分子 #6: Triplex capsid protein 1
分子 | 名称: Triplex capsid protein 1 / タイプ: protein_or_peptide / ID: 6 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() 株: B95-8 |
分子量 | 理論値: 39.231539 KDa |
配列 | 文字列: MKVQGSVDRR RLQRRIAGLL PPPARRLNIS RGSEFTRDVR GLVEEHAQAS SLSAAAVWRA GLLAPGEVAV AGGGSGGGSF SWSGWRPPV FGDFLIHASS FNNAEATGTP LFQFKQSDPF SGVDAVFTPL SLFILMNHGR GVAARVEAGG GLTRMANLLY D SPATLADL ...文字列: MKVQGSVDRR RLQRRIAGLL PPPARRLNIS RGSEFTRDVR GLVEEHAQAS SLSAAAVWRA GLLAPGEVAV AGGGSGGGSF SWSGWRPPV FGDFLIHASS FNNAEATGTP LFQFKQSDPF SGVDAVFTPL SLFILMNHGR GVAARVEAGG GLTRMANLLY D SPATLADL VPDFGRLVAD RRFHNFITPV GPLVENIKST YLNKITTVVH GPVVSKAIPR STVKVTVPQE AFVDLDAWLS GG AGGGGGV CFVGGLGLQP CPADARLYVA LTYEEAGPRF TFFQSSRGHC QIMNILRIYY SPSIMHRYAV VQPLHIEELT FGA VACLGT FSATDGWRRS AFNYRGSSLP VVEIDSFYSN VSDWEVIL UniProtKB: Triplex capsid protein 1 |
-分子 #7: Triplex capsid protein 2
分子 | 名称: Triplex capsid protein 2 / タイプ: protein_or_peptide / ID: 7 / コピー数: 4 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() 株: B95-8 |
分子量 | 理論値: 33.654039 KDa |
配列 | 文字列: MDLKVVVSLS SRLYTDEIAK MQQRIGCILP LASTHGTQNV QGLGLGQVYS LETVPDYVSM YNYLSDCTLA VLDEVSVDSL ILTKIVPGQ TYAIKNKYQP FFQWHGTGSL SVMPPVFGRE HATVKLESND VDIVFPMVLP TPIAEEVLQK ILLFNVYSRV V MQAPGNAD ...文字列: MDLKVVVSLS SRLYTDEIAK MQQRIGCILP LASTHGTQNV QGLGLGQVYS LETVPDYVSM YNYLSDCTLA VLDEVSVDSL ILTKIVPGQ TYAIKNKYQP FFQWHGTGSL SVMPPVFGRE HATVKLESND VDIVFPMVLP TPIAEEVLQK ILLFNVYSRV V MQAPGNAD MLDVHMHLGS VSYLGHHYEL ALPEVPGPLG LALLDNLSLY FCIMVTLLPR ASMRLVRGLI RHEHHDLLNL FQ EMVPDEI ARIDLDDLSV ADDLSRMRVM MTYLQSLASL FNLGPRLATA AYSQETLTAT CWLR UniProtKB: Triplex capsid protein 2 |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 QUANTUM (4k x 4k) 平均電子線量: 48.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: OTHER / 撮影モード: OTHER |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |