+Open data
-Basic information
Entry | Database: PDB / ID: 2fv9 | ||||||
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Title | Crystal structure of TACE in complex with JMV 390-1 | ||||||
Components | ADAM 17 | ||||||
Keywords | HYDROLASE / TACE ADAM33 ZN-ENDOPEPTIDASE | ||||||
Function / homology | Function and homology information ADAM 17 endopeptidase / regulation of mast cell apoptotic process / signal release / metalloendopeptidase activity involved in amyloid precursor protein catabolic process / cellular response to high density lipoprotein particle stimulus / positive regulation of epidermal growth factor-activated receptor activity / Constitutive Signaling by NOTCH1 t(7;9)(NOTCH1:M1580_K2555) Translocation Mutant / tumor necrosis factor binding / interleukin-6 receptor binding / Notch receptor processing ...ADAM 17 endopeptidase / regulation of mast cell apoptotic process / signal release / metalloendopeptidase activity involved in amyloid precursor protein catabolic process / cellular response to high density lipoprotein particle stimulus / positive regulation of epidermal growth factor-activated receptor activity / Constitutive Signaling by NOTCH1 t(7;9)(NOTCH1:M1580_K2555) Translocation Mutant / tumor necrosis factor binding / interleukin-6 receptor binding / Notch receptor processing / positive regulation of T cell chemotaxis / TNF signaling / positive regulation of leukocyte chemotaxis / germinal center formation / Regulated proteolysis of p75NTR / Release of Hh-Np from the secreting cell / commissural neuron axon guidance / positive regulation of tumor necrosis factor-mediated signaling pathway / neutrophil mediated immunity / wound healing, spreading of epidermal cells / negative regulation of cold-induced thermogenesis / Notch binding / CD163 mediating an anti-inflammatory response / positive regulation of vascular endothelial cell proliferation / cell adhesion mediated by integrin / positive regulation of epidermal growth factor receptor signaling pathway / Signaling by EGFR / cytokine binding / amyloid precursor protein catabolic process / Collagen degradation / membrane protein ectodomain proteolysis / positive regulation of cyclin-dependent protein serine/threonine kinase activity / positive regulation of blood vessel endothelial cell migration / positive regulation of G1/S transition of mitotic cell cycle / Growth hormone receptor signaling / Nuclear signaling by ERBB4 / positive regulation of chemokine production / spleen development / Notch signaling pathway / Constitutive Signaling by NOTCH1 HD Domain Mutants / B cell differentiation / Activated NOTCH1 Transmits Signal to the Nucleus / cell motility / PDZ domain binding / negative regulation of transforming growth factor beta receptor signaling pathway / protein processing / metalloendopeptidase activity / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / SH3 domain binding / positive regulation of tumor necrosis factor production / metallopeptidase activity / actin cytoskeleton / integrin binding / peptidase activity / T cell differentiation in thymus / positive regulation of cell growth / endopeptidase activity / response to lipopolysaccharide / response to hypoxia / cell adhesion / defense response to Gram-positive bacterium / positive regulation of cell migration / response to xenobiotic stimulus / apical plasma membrane / membrane raft / endoplasmic reticulum lumen / Golgi membrane / positive regulation of cell population proliferation / cell surface / proteolysis / membrane / metal ion binding / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.02 Å | ||||||
Authors | Orth, P. | ||||||
Citation | Journal: Protein Eng.Des.Sel. / Year: 2006 Title: Stabilization of the autoproteolysis of TNF-alpha converting enzyme (TACE) results in a novel crystal form suitable for structure-based drug design studies. Authors: Ingram, R.N. / Orth, P. / Strickland, C.L. / Le, H.V. / Madison, V. / Beyer, B.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2fv9.cif.gz | 115.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2fv9.ent.gz | 87.6 KB | Display | PDB format |
PDBx/mmJSON format | 2fv9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2fv9_validation.pdf.gz | 1 MB | Display | wwPDB validaton report |
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Full document | 2fv9_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 2fv9_validation.xml.gz | 21.9 KB | Display | |
Data in CIF | 2fv9_validation.cif.gz | 30.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fv/2fv9 ftp://data.pdbj.org/pub/pdb/validation_reports/fv/2fv9 | HTTPS FTP |
-Related structure data
Related structure data | 2ddfC 1bkcS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 29123.594 Da / Num. of mol.: 2 / Mutation: S266A, V353G, Q452N Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ADAM17, CSVP, TACE / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: P78536, ADAM 17 endopeptidase #2: Chemical | #3: Chemical | ChemComp-INN / | #4: Chemical | ChemComp-002 / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 49.98 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion / pH: 5.6 Details: 15% PEG 6k, 10% 2-Propanol, 100 mM sodium citrate, pH 5.6, VAPOR DIFFUSION, temperature 295K |
-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Dec 15, 2002 |
Radiation | Monochromator: osmic mirror green / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.02→50 Å / Num. all: 38421 / Num. obs: 35008 / % possible obs: 90.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.047 / Χ2: 1.062 / Net I/σ(I): 16.7 |
Reflection shell | Resolution: 2.02→2.09 Å / % possible obs: 91.7 % / Rmerge(I) obs: 0.513 / Num. unique obs: 3461 / Χ2: 1.034 / % possible all: 91.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB Entry: 1BKC Resolution: 2.02→20 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 42.5 Å2
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Refinement step | Cycle: LAST / Resolution: 2.02→20 Å
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Xplor file |
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