National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
124279
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
118047
米国
National Science Foundation (NSF, United States)
DMR-2011401
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
124165
米国
National Institutes of Health/National Institute on Drug Abuse (NIH/NIDA)
007097
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
150351
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
83196
米国
引用
ジャーナル: J Mol Biol / 年: 2023 タイトル: HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus Maturation. 著者: Nathaniel Talledge / Huixin Yang / Ke Shi / Raffaele Coray / Guichuan Yu / William G Arndt / Shuyu Meng / Gloria C Baxter / Luiza M Mendonça / Daniel Castaño-Díez / Hideki Aihara / Louis M ...著者: Nathaniel Talledge / Huixin Yang / Ke Shi / Raffaele Coray / Guichuan Yu / William G Arndt / Shuyu Meng / Gloria C Baxter / Luiza M Mendonça / Daniel Castaño-Díez / Hideki Aihara / Louis M Mansky / Wei Zhang / 要旨: Retrovirus immature particle morphology consists of a membrane enclosed, pleomorphic, spherical and incomplete lattice of Gag hexamers. Previously, we demonstrated that human immunodeficiency virus ...Retrovirus immature particle morphology consists of a membrane enclosed, pleomorphic, spherical and incomplete lattice of Gag hexamers. Previously, we demonstrated that human immunodeficiency virus type 2 (HIV-2) immature particles possess a distinct and extensive Gag lattice morphology. To better understand the nature of the continuously curved hexagonal Gag lattice, we have used the single particle cryo-electron microscopy method to determine the HIV-2 Gag lattice structure for immature virions. The reconstruction map at 5.5 Å resolution revealed a stable, wineglass-shaped Gag hexamer structure with structural features consistent with other lentiviral immature Gag lattice structures. Cryo-electron tomography provided evidence for nearly complete ordered Gag lattice structures in HIV-2 immature particles. We also solved a 1.98 Å resolution crystal structure of the carboxyl-terminal domain (CTD) of the HIV-2 capsid (CA) protein that identified a structured helix 12 supported via an interaction of helix 10 in the absence of the SP1 region of Gag. Residues at the helix 10-12 interface proved critical in maintaining HIV-2 particle release and infectivity. Taken together, our findings provide the first 3D organization of HIV-2 immature Gag lattice and important insights into both HIV Gag lattice stabilization and virus maturation.
全体 : Human immunodeficiency virus type 2 (ISOLATE ROD)
全体
名称: Human immunodeficiency virus type 2 (ISOLATE ROD) (ヒト免疫不全ウイルス)
要素
ウイルス: Human immunodeficiency virus type 2 (ISOLATE ROD) (ヒト免疫不全ウイルス)
タンパク質・ペプチド: HIV-2 Immature Gag
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超分子 #1: Human immunodeficiency virus type 2 (ISOLATE ROD)
超分子
名称: Human immunodeficiency virus type 2 (ISOLATE ROD) / タイプ: virus / ID: 1 / 親要素: 0 / 含まれる分子: all 詳細: HIV-2 VLPs generated by Gag co-overexpressed with HIV-2 Env in Hek293T cells and purified from media supernatant NCBI-ID: 11720 生物種: Human immunodeficiency virus type 2 (ISOLATE ROD) ウイルスタイプ: VIRUS-LIKE PARTICLE / ウイルス・単離状態: STRAIN / ウイルス・エンベロープ: Yes / ウイルス・中空状態: No
凍結剤: ETHANE / チャンバー内湿度: 85 % / チャンバー内温度: 292.15 K / 装置: LEICA EM GP / 詳細: Leica GP2 Grid Plunger..
切片作成
その他: NO SECTIONING
位置合わせマーカー
Manufacturer: Ted pella / 直径: 10 nm
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電子顕微鏡法
顕微鏡
FEI TITAN KRIOS
特殊光学系
エネルギーフィルター - スリット幅: 20 eV
撮影
フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: SUPER-RESOLUTION / デジタル化 - 画像ごとのフレーム数: 1-8 / 撮影したグリッド数: 2 / 実像数: 63 / 平均露光時間: 2.0 sec. / 平均電子線量: 1.1 e/Å2 詳細: Two second exposures with a 0.25 frames/sec rate per tilt with a 1.1 e-/Angstrom^2 dose per tilt with a total tilt series dose of 45 e-/Angstroms^2