[English] 日本語
Yorodumi- EMDB-2845: 40S-eIF1-eIF1A-eIF3-eIF3j translation initiation complex from Lac... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-2845 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | 40S-eIF1-eIF1A-eIF3-eIF3j translation initiation complex from Lachancea kluyveri | |||||||||
Map data | Structure of the Lachancea kluyveri 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex | |||||||||
Sample |
| |||||||||
Keywords | eIF3 / translation initiation | |||||||||
Function / homology | Function and homology information eukaryotic translation initiation factor 3 complex / formation of cytoplasmic translation initiation complex / eukaryotic 43S preinitiation complex / eukaryotic 48S preinitiation complex / translation initiation factor binding / translation initiation factor activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA processing / ribosomal small subunit assembly / small ribosomal subunit ...eukaryotic translation initiation factor 3 complex / formation of cytoplasmic translation initiation complex / eukaryotic 43S preinitiation complex / eukaryotic 48S preinitiation complex / translation initiation factor binding / translation initiation factor activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA processing / ribosomal small subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / RNA binding / zinc ion binding / metal ion binding / cytoplasm Similarity search - Function | |||||||||
Biological species | Lachancea kluyveri (fungus) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 6.47 Å | |||||||||
Authors | Aylett CHS / Boehringer D / Erzberger JP / Schaefer T / Ban N | |||||||||
Citation | Journal: Nat Struct Mol Biol / Year: 2015 Title: Structure of a yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex. Authors: Christopher H S Aylett / Daniel Boehringer / Jan P Erzberger / Tanja Schaefer / Nenad Ban / Abstract: Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing ...Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits. | |||||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_2845.map.gz | 57.4 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-2845-v30.xml emd-2845.xml | 21.2 KB 21.2 KB | Display Display | EMDB header |
Images | emdb-2845.png | 127.4 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-2845 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-2845 | HTTPS FTP |
-Validation report
Summary document | emd_2845_validation.pdf.gz | 233 KB | Display | EMDB validaton report |
---|---|---|---|---|
Full document | emd_2845_full_validation.pdf.gz | 232.1 KB | Display | |
Data in XML | emd_2845_validation.xml.gz | 6.1 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-2845 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-2845 | HTTPS FTP |
-Related structure data
Related structure data | 4uerMC M: atomic model generated by this map C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|---|
Related items in Molecule of the Month |
-Map
File | Download / File: emd_2845.map.gz / Format: CCP4 / Size: 62.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Structure of the Lachancea kluyveri 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.39 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|