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Yorodumi- EMDB-27638: Structure of EBOV GP lacking the mucin-like domain with 9.20.1A2 ... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-27638 | |||||||||
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Title | Structure of EBOV GP lacking the mucin-like domain with 9.20.1A2 Fab and 6D6 scFv bound | |||||||||
Map data | Structure of EBOV GP lacking the mucin-like domain with 9.20.1A2 Fab and 6D6 scFv bound | |||||||||
Sample |
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Keywords | Antibody / Viral protein / glycoprotein / immune system / Ebola virus / VIRAL PROTEIN-Immune System complex | |||||||||
Function / homology | Function and homology information host cell endoplasmic reticulum / viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / suppression by virus of host tetherin activity / host cell cytoplasm / entry receptor-mediated virion attachment to host cell / symbiont-mediated suppression of host innate immune response / symbiont entry into host cell / membrane raft / fusion of virus membrane with host endosome membrane ...host cell endoplasmic reticulum / viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / suppression by virus of host tetherin activity / host cell cytoplasm / entry receptor-mediated virion attachment to host cell / symbiont-mediated suppression of host innate immune response / symbiont entry into host cell / membrane raft / fusion of virus membrane with host endosome membrane / lipid binding / viral envelope / host cell plasma membrane / virion membrane / extracellular region / identical protein binding / membrane Similarity search - Function | |||||||||
Biological species | Ebola virus - Mayinga, Zaire, 1976 / Homo sapiens (human) / Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.0 Å | |||||||||
Authors | Yu X / Saphire EO | |||||||||
Funding support | United States, 2 items
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Citation | Journal: Cell Rep / Year: 2023 Title: The evolution and determinants of neutralization of potent head-binding antibodies against Ebola virus. Authors: Xiaoying Yu / Kathryn M Hastie / Carl W Davis / Ruben Diaz Avalos / Dewight Williams / Diptiben Parekh / Sean Hui / Colin Mann / Chitra Hariharan / Ayato Takada / Rafi Ahmed / Erica Ollmann Saphire / Abstract: Monoclonal antibodies against the Ebola virus (EBOV) surface glycoprotein are effective treatments for EBOV disease. Antibodies targeting the EBOV glycoprotein (GP) head epitope have potent ...Monoclonal antibodies against the Ebola virus (EBOV) surface glycoprotein are effective treatments for EBOV disease. Antibodies targeting the EBOV glycoprotein (GP) head epitope have potent neutralization and Fc effector function activity and thus are of high interest as therapeutics and for vaccine design. Here we focus on the head-binding antibodies 1A2 and 1D5, which have been identified previously in a longitudinal study of survivors of EBOV infection. 1A2 and 1D5 have the same heavy- and light-chain germlines despite being isolated from different individuals and at different time points after recovery from infection. Cryoelectron microscopy analysis of each antibody in complex with the EBOV surface GP reveals key amino acid substitutions in 1A2 that contribute to greater affinity, improved neutralization potency, and enhanced breadth as well as two strategies for antibody evolution from a common site. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_27638.map.gz | 170.8 MB | EMDB map data format | |
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Header (meta data) | emd-27638-v30.xml emd-27638.xml | 19.6 KB 19.6 KB | Display Display | EMDB header |
Images | emd_27638.png | 49.1 KB | ||
Filedesc metadata | emd-27638.cif.gz | 6.3 KB | ||
Others | emd_27638_half_map_1.map.gz emd_27638_half_map_2.map.gz | 171.1 MB 171.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-27638 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-27638 | HTTPS FTP |
-Validation report
Summary document | emd_27638_validation.pdf.gz | 901.3 KB | Display | EMDB validaton report |
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Full document | emd_27638_full_validation.pdf.gz | 900.9 KB | Display | |
Data in XML | emd_27638_validation.xml.gz | 15.3 KB | Display | |
Data in CIF | emd_27638_validation.cif.gz | 18.2 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-27638 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-27638 | HTTPS FTP |
-Related structure data
Related structure data | 8dpmMC 8dplC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_27638.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | Structure of EBOV GP lacking the mucin-like domain with 9.20.1A2 Fab and 6D6 scFv bound | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.05 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half Map 1
File | emd_27638_half_map_1.map | ||||||||||||
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Annotation | Half Map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half Map 2
File | emd_27638_half_map_2.map | ||||||||||||
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Annotation | Half Map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Complex of Ebola virus glycoprotein trimer with 1A2 antibody Fab ...
Entire | Name: Complex of Ebola virus glycoprotein trimer with 1A2 antibody Fab and 6D6 antibody scFv |
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Components |
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-Supramolecule #1: Complex of Ebola virus glycoprotein trimer with 1A2 antibody Fab ...
Supramolecule | Name: Complex of Ebola virus glycoprotein trimer with 1A2 antibody Fab and 6D6 antibody scFv type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5 |
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Source (natural) | Organism: Ebola virus - Mayinga, Zaire, 1976 |
-Supramolecule #2: Antibody 9.20.1A2 Fab
Supramolecule | Name: Antibody 9.20.1A2 Fab / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #4-#5 |
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Source (natural) | Organism: Homo sapiens (human) |
-Macromolecule #1: Glycoprotein GP1
Macromolecule | Name: Glycoprotein GP1 / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Ebola virus - Mayinga, Zaire, 1976 / Strain: Mayinga-76 |
Molecular weight | Theoretical: 31.297158 KDa |
Recombinant expression | Organism: Drosophila melanogaster (fruit fly) |
Sequence | String: IPLGVIHNST LQVSDVDKLV CRDKLSSTNQ LRSVGLNLEG NGVATDVPSA TKRWGFRSGV PPKVVNYEAG EWAENCYNLE IKKPDGSEC LPAAPDGIRG FPRCRYVHKV SGTGPCAGDF AFHKEGAFFL YDRLASTVIY RGTTFAEGVV AFLILPQAKK D FFSSHPLR ...String: IPLGVIHNST LQVSDVDKLV CRDKLSSTNQ LRSVGLNLEG NGVATDVPSA TKRWGFRSGV PPKVVNYEAG EWAENCYNLE IKKPDGSEC LPAAPDGIRG FPRCRYVHKV SGTGPCAGDF AFHKEGAFFL YDRLASTVIY RGTTFAEGVV AFLILPQAKK D FFSSHPLR EPVNATEDPS SGYYSTTIRY QATGFGTNET EYLFEVDNLT YVQLESRFTP QFLLQLNETI YTSGKRSNTT GK LIWKVNP EIDTTIGEWA FWETKKNLTR KIRSEELSFT VVS UniProtKB: Envelope glycoprotein |
-Macromolecule #2: Glycoprotein GP2
Macromolecule | Name: Glycoprotein GP2 / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Ebola virus - Mayinga, Zaire, 1976 |
Molecular weight | Theoretical: 15.335362 KDa |
Recombinant expression | Organism: Drosophila melanogaster (fruit fly) |
Sequence | String: EAIVNAQPKC NPNLHYWTTQ DEGAAIGLAW IPYFGPAAEG IYTEGLMHNQ DGLICGLRQL ANETTQALQL FLRATTELRT FSILNRKAI DFLLQRWGGT CHILGPDCCI EPHDWTKNIT DKIDQIIHDF VDKTLPD UniProtKB: Envelope glycoprotein |
-Macromolecule #3: Antibody 6D6 scFv
Macromolecule | Name: Antibody 6D6 scFv / type: protein_or_peptide / ID: 3 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 25.853459 KDa |
Recombinant expression | Organism: Drosophila melanogaster (fruit fly) |
Sequence | String: QVQLQQSGTE LVKPGASVKL SCKASGYTFT SYWMHWVKQR PGQGLEWIGE INPRNGRTDF SEKFKSKATL TVDTSSSTAF IQLSSLTSE DSAVYYCARW GYYGSSDYWG QGTALTVSSG TGGSGGGGSG GGGSGGGASD IVVTQSHKFM STSVGDRVSI T CKASQDVS ...String: QVQLQQSGTE LVKPGASVKL SCKASGYTFT SYWMHWVKQR PGQGLEWIGE INPRNGRTDF SEKFKSKATL TVDTSSSTAF IQLSSLTSE DSAVYYCARW GYYGSSDYWG QGTALTVSSG TGGSGGGGSG GGGSGGGASD IVVTQSHKFM STSVGDRVSI T CKASQDVS VAVAWYQQKT GQSPKLLIYS ASYRITGVPD RFTGSGSGTD FTFTISSVQA EDMAVYYCQQ HYSTPPWTFG GG TKL |
-Macromolecule #4: Antibody 9.20.1A2 Fab heavy chain
Macromolecule | Name: Antibody 9.20.1A2 Fab heavy chain / type: protein_or_peptide / ID: 4 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 13.340016 KDa |
Recombinant expression | Organism: Cricetulus griseus (Chinese hamster) |
Sequence | String: EVQLVESGGD LVQPGGSLRL SCAASGITLS GVWMNWVRQA PGKGLEWIGR IKSTSDGGRA DFAAPARGRF TMSRDESKNK LFLQMNNLG IEDTGMYYCF TRVQRDGTKD DFWGRGTLVT VSS |
-Macromolecule #5: Antibody 9.20.1A2 Fab light chain
Macromolecule | Name: Antibody 9.20.1A2 Fab light chain / type: protein_or_peptide / ID: 5 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 11.985138 KDa |
Recombinant expression | Organism: Cricetulus griseus (Chinese hamster) |
Sequence | String: QSVLTQPPSV SGAPGQTVTI SCTGSYSNIG AGYDVQWYQH LPGTAPKLLI YDNVHRPSGV PDRFSGSKSG TSASLAITGL QTEDEADYY CQSYDSRLRD QWVFGGGTKL TVL |
-Macromolecule #7: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 7 / Number of copies: 3 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ChemComp-NAG: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 55.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: INSILICO MODEL |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 81175 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |