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- EMDB-27386: Cryo-EM structure of the zebrafish two pore domain K+ channel TRE... -
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Open data
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Basic information
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Title | Cryo-EM structure of the zebrafish two pore domain K+ channel TREK1 (K2P2.1) in DDM detergent | |||||||||
![]() | drTREK1 in DDM detergent map | |||||||||
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Function / homology | : ![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.27 Å | |||||||||
![]() | Schmidpeter PAM / Nimigean CM / Riegelhaupt PM | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Membrane phospholipids control gating of the mechanosensitive potassium leak channel TREK1. Authors: Philipp A M Schmidpeter / John T Petroff / Leila Khajoueinejad / Aboubacar Wague / Cheryl Frankfater / Wayland W L Cheng / Crina M Nimigean / Paul M Riegelhaupt / ![]() Abstract: Tandem pore domain (K2P) potassium channels modulate resting membrane potentials and shape cellular excitability. For the mechanosensitive subfamily of K2Ps, the composition of phospholipids within ...Tandem pore domain (K2P) potassium channels modulate resting membrane potentials and shape cellular excitability. For the mechanosensitive subfamily of K2Ps, the composition of phospholipids within the bilayer strongly influences channel activity. To examine the molecular details of K2P lipid modulation, we solved cryo-EM structures of the TREK1 K2P channel bound to either the anionic lipid phosphatidic acid (PA) or the zwitterionic lipid phosphatidylethanolamine (PE). At the extracellular face of TREK1, a PA lipid inserts its hydrocarbon tail into a pocket behind the selectivity filter, causing a structural rearrangement that recapitulates mutations and pharmacology known to activate TREK1. At the cytoplasmic face, PA and PE lipids compete to modulate the conformation of the TREK1 TM4 gating helix. Our findings demonstrate two distinct pathways by which anionic lipids enhance TREK1 activity and provide a framework for a model that integrates lipid gating with the effects of other mechanosensitive K2P modulators. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 20.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 17.8 KB 17.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 6.9 KB | Display | ![]() |
Images | ![]() | 1.4 MB | ||
Masks | ![]() | 27 MB | ![]() | |
Others | ![]() ![]() | 20.7 MB 20.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8de7MC ![]() 8de8C ![]() 8de9C M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | drTREK1 in DDM detergent map | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.076 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: drTREK1 in DDM detergent halfmap1
File | emd_27386_half_map_1.map | ||||||||||||
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Annotation | drTREK1 in DDM detergent halfmap1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: drTREK1 in DDM detergent halfmap2
File | emd_27386_half_map_2.map | ||||||||||||
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Annotation | drTREK1 in DDM detergent halfmap2 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Potassium channel subfamily K member 2
Entire | Name: Potassium channel subfamily K member 2 |
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Components |
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-Supramolecule #1: Potassium channel subfamily K member 2
Supramolecule | Name: Potassium channel subfamily K member 2 / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Potassium channel, subfamily K, member 2a
Macromolecule | Name: Potassium channel, subfamily K, member 2a / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 35.559383 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MAAPDLLDPK SATHNTKPRL SFSSKPIVYN SGDDCESITT VMKWKTVLAI FLLVVLYLII GATVFKALEQ PEEGLQKYRI IQEKIDFLS MHTCVQTSEL EDLVKQVVLA IRAGVNPSGH PSQESSMWDL SSSFFFAGTV ITTIGFGNVS PHTEGGRIFC I IYALLGIP ...String: MAAPDLLDPK SATHNTKPRL SFSSKPIVYN SGDDCESITT VMKWKTVLAI FLLVVLYLII GATVFKALEQ PEEGLQKYRI IQEKIDFLS MHTCVQTSEL EDLVKQVVLA IRAGVNPSGH PSQESSMWDL SSSFFFAGTV ITTIGFGNVS PHTEGGRIFC I IYALLGIP LFGFLLAGVG DQLGTIFGKG IAKVEKMFVK WNVSQTKIRV TSTVLFILFG CLLFVALPAL IFQHIEGWSA LE SIYFVVI TLTTIGFGDF VAGGSEIEYL DYYKPIVWFW ILVGLAYFAA VLSMIGDWLR VISKKTKEEV GEFRAHAAEW TAN V |
-Macromolecule #2: DODECYL-BETA-D-MALTOSIDE
Macromolecule | Name: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 2 / Number of copies: 1 / Formula: LMT |
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Molecular weight | Theoretical: 510.615 Da |
Chemical component information | ![]() ChemComp-LMT: |
-Macromolecule #3: POTASSIUM ION
Macromolecule | Name: POTASSIUM ION / type: ligand / ID: 3 / Number of copies: 3 / Formula: K |
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Molecular weight | Theoretical: 39.098 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 3 mg/mL |
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Buffer | pH: 8 / Details: 150mM KCl, 20mM TRIS, 0.25mM DDM |
Grid | Model: UltrAuFoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 80 sec. / Pretreatment - Pressure: 0.03 kPa |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 294 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | TFS KRIOS |
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Software | Name: Leginon |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 51.83 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 64000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |