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- EMDB-27174: Cryo-electron microscopy structure of human kidney Fructose-bisph... -
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Open data
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Basic information
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Title | Cryo-electron microscopy structure of human kidney Fructose-bisphosphate aldolase B | |||||||||
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![]() | fructose-bisphosphate aldolase B / ALDOB / ![]() | |||||||||
Function / homology | ![]() fructose-1-phosphate aldolase activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Lyu M / Yu EW | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-electron microscopy structure of human kidney Fructose-bisphosphate aldolase B Authors: Lyu M / Yu EW | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 97.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.3 KB 13.3 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 13.7 KB | Display | ![]() |
Images | ![]() | 105.2 KB | ||
Filedesc metadata | ![]() | 5.1 KB | ||
Others | ![]() ![]() | 95.3 MB 95.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8d44MC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.07 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_27174_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_27174_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Fructose-bisphosphate aldolase B
Entire | Name: Fructose-bisphosphate aldolase B |
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Components |
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-Supramolecule #1: Fructose-bisphosphate aldolase B
Supramolecule | Name: Fructose-bisphosphate aldolase B / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Fructose-bisphosphate aldolase B
Macromolecule | Name: Fructose-bisphosphate aldolase B / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO / EC number: ![]() |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 39.519969 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MAHRFPALTQ EQKKELSEIA QSIVANGKGI LAADESVGTM GNRLQRIKVE NTEENRRQFR EILFSVDSSI NQSIGGVILF HETLYQKDS QGKLFRNILK EKGIVVGIKL DQGGAPLAGT NKETTIQGLD GLSERCAQYK KDGVDFGKWR AVLRIADQCP S SLAIQENA ...String: MAHRFPALTQ EQKKELSEIA QSIVANGKGI LAADESVGTM GNRLQRIKVE NTEENRRQFR EILFSVDSSI NQSIGGVILF HETLYQKDS QGKLFRNILK EKGIVVGIKL DQGGAPLAGT NKETTIQGLD GLSERCAQYK KDGVDFGKWR AVLRIADQCP S SLAIQENA NALARYASIC QQNGLVPIVE PEVIPDGDHD LEHCQYVTEK VLAAVYKALN DHHVYLEGTL LKPNMVTAGH AC TKKYTPE QVAMATVTAL HRTVPAAVPG ICFLSGGMSE EDATLNLNAI NLCPLPKPWK LSFSYGRALQ ASALAAWGGK AAN KEATQE AFMKRAMANC QAAKGQYVHT GSSGAASTQS LFTACYTY UniProtKB: Fructose-bisphosphate aldolase B |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Concentration | .7 mg/mL |
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Buffer | pH: 7.5 |
Grid | Model: Quantifoil R1.2/1.3 / Support film - Material: CARBON / Support film - topology: HOLEY |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.5 K / Instrument: FEI VITROBOT MARK I / Details: blot 15 seconds before plunging. |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 39.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Protocol: AB INITIO MODEL |
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Output model | ![]() PDB-8d44: |