登録情報 データベース : EMDB / ID : EMD-27164 ダウンロードとリンクタイトル Human alpha3 Na+/K+-ATPase in its Na+-occluded state マップデータHuman alpha3 Na+/K+-ATPase in its Na+-occluded state 詳細 試料複合体 : Na+/K+-ATPaseタンパク質・ペプチド : Sodium/potassium-transporting ATPase subunit alpha-3タンパク質・ペプチド : Sodium/potassium-transporting ATPase subunit beta-1タンパク質・ペプチド : FXYD domain-containing ion transport regulator 6リガンド : ADENOSINE-5'-DIPHOSPHATEリガンド : TETRAFLUOROALUMINATE ION 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
neuron to neuron synapse / protein transport into plasma membrane raft / positive regulation of calcium:sodium antiporter activity / positive regulation of potassium ion transmembrane transporter activity / positive regulation of P-type sodium:potassium-exchanging transporter activity / Na+/K+-exchanging ATPase / regulation of monoatomic ion transport / positive regulation of sodium ion export across plasma membrane / positive regulation of potassium ion import across plasma membrane / response to glycoside ... neuron to neuron synapse / protein transport into plasma membrane raft / positive regulation of calcium:sodium antiporter activity / positive regulation of potassium ion transmembrane transporter activity / positive regulation of P-type sodium:potassium-exchanging transporter activity / Na+/K+-exchanging ATPase / regulation of monoatomic ion transport / positive regulation of sodium ion export across plasma membrane / positive regulation of potassium ion import across plasma membrane / response to glycoside / membrane repolarization during cardiac muscle cell action potential / photoreceptor inner segment membrane / regulation of resting membrane potential / P-type sodium:potassium-exchanging transporter activity / steroid hormone binding / membrane repolarization / sodium:potassium-exchanging ATPase complex / establishment or maintenance of transmembrane electrochemical gradient / sodium ion export across plasma membrane / cell communication by electrical coupling involved in cardiac conduction / intracellular potassium ion homeostasis / intracellular sodium ion homeostasis / positive regulation of ATP-dependent activity / regulation of cardiac muscle contraction by calcium ion signaling / relaxation of cardiac muscle / Basigin interactions / cellular response to steroid hormone stimulus / organelle membrane / sodium ion transport / neuronal cell body membrane / potassium ion import across plasma membrane / monoatomic cation transmembrane transport / Ion transport by P-type ATPases / ATPase activator activity / intercalated disc / lateral plasma membrane / sperm flagellum / sodium ion transmembrane transport / sodium channel regulator activity / cardiac muscle contraction / ATP metabolic process / Ion homeostasis / T-tubule / neuron projection maintenance / photoreceptor inner segment / proton transmembrane transport / potassium ion transport / protein localization to plasma membrane / sarcolemma / intracellular calcium ion homeostasis / cellular response to amyloid-beta / extracellular vesicle / MHC class II protein complex binding / ATPase binding / regulation of gene expression / presynaptic membrane / protein-macromolecule adaptor activity / amyloid-beta binding / protein-folding chaperone binding / basolateral plasma membrane / Potential therapeutics for SARS / postsynaptic membrane / protein stabilization / cell adhesion / apical plasma membrane / protein heterodimerization activity / axon / innate immune response / neuronal cell body / glutamatergic synapse / synapse / protein kinase binding / Golgi apparatus / endoplasmic reticulum / ATP hydrolysis activity / extracellular exosome / ATP binding / membrane / metal ion binding / plasma membrane 類似検索 - 分子機能 : / Ion-transport regulator, FXYD motif / ATP1G1/PLM/MAT8 family / FXYD family signature. / : / Sodium/potassium-transporting ATPase subunit beta / Sodium/potassium-transporting ATPase subunit beta superfamily / Sodium / potassium ATPase beta chain / Sodium and potassium ATPases beta subunits signature 1. / Sodium and potassium ATPases beta subunits signature 2. ... : / Ion-transport regulator, FXYD motif / ATP1G1/PLM/MAT8 family / FXYD family signature. / : / Sodium/potassium-transporting ATPase subunit beta / Sodium/potassium-transporting ATPase subunit beta superfamily / Sodium / potassium ATPase beta chain / Sodium and potassium ATPases beta subunits signature 1. / Sodium and potassium ATPases beta subunits signature 2. / P-type ATPase subfamily IIC, subunit alpha / Cation-transporting P-type ATPase, C-terminal / Cation transporting ATPase, C-terminus / Cation transporter/ATPase, N-terminus / Cation-transporting P-type ATPase, N-terminal / Cation transporter/ATPase, N-terminus / Cation transport ATPase (P-type) / E1-E2 ATPase / P-type ATPase, haloacid dehalogenase domain / P-type ATPase, phosphorylation site / P-type ATPase, cytoplasmic domain N / E1-E2 ATPases phosphorylation site. / P-type ATPase, A domain superfamily / P-type ATPase / P-type ATPase, transmembrane domain superfamily / HAD superfamily / HAD-like superfamily 類似検索 - ドメイン・相同性 Sodium/potassium-transporting ATPase subunit beta-1 / Sodium/potassium-transporting ATPase subunit alpha-3 / FXYD domain-containing ion transport regulator 6 類似検索 - 構成要素生物種 Homo sapiens (ヒト)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.7 Å 詳細 データ登録者Nguyen PT / Bai X 資金援助 米国, 2件 詳細 詳細を隠すOrganization Grant number 国 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01GM136976 米国 Welch Foundation I-1944 米国
引用ジャーナル : Nat Commun / 年 : 2022タイトル : Structural basis for gating mechanism of the human sodium-potassium pump.著者 : Phong T Nguyen / Christine Deisl / Michael Fine / Trevor S Tippetts / Emiko Uchikawa / Xiao-Chen Bai / Beth Levine / 要旨 : P2-type ATPase sodium-potassium pumps (Na/K-ATPases) are ion-transporting enzymes that use ATP to transport Na and K on opposite sides of the lipid bilayer against their electrochemical gradients to ... P2-type ATPase sodium-potassium pumps (Na/K-ATPases) are ion-transporting enzymes that use ATP to transport Na and K on opposite sides of the lipid bilayer against their electrochemical gradients to maintain ion concentration gradients across the membranes in all animal cells. Despite the available molecular architecture of the Na/K-ATPases, a complete molecular mechanism by which the Na and K ions access into and are released from the pump remains unknown. Here we report five cryo-electron microscopy (cryo-EM) structures of the human alpha3 Na/K-ATPase in its cytoplasmic side-open (E1), ATP-bound cytoplasmic side-open (E1•ATP), ADP-AlF trapped Na-occluded (E1•P-ADP), BeF trapped exoplasmic side-open (E2P) and MgF trapped K-occluded (E2•P) states. Our work reveals the atomically resolved structural detail of the cytoplasmic gating mechanism of the Na/K-ATPase. 履歴 登録 2022年6月1日 - ヘッダ(付随情報) 公開 2022年9月21日 - マップ公開 2022年9月21日 - 更新 2022年9月21日 - 現状 2022年9月21日 処理サイト : RCSB / 状態 : 公開
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