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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Photosynthetic assembly of Chlorobaculum tepidum (RC-FMO1) | |||||||||
![]() | RC-FMO1 map | |||||||||
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![]() | FMO / reaction center / single-particle cryo-EM / bacteriochlorophyll / photosynthesis / electron transport chain / energy transfer | |||||||||
Function / homology | ![]() thylakoid / bacteriochlorophyll binding / iron-sulfur cluster binding / photosynthesis / electron transfer activity / heme binding / membrane / metal ion binding / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.49 Å | |||||||||
![]() | Puskar R / Truong CD / Swain K / Li S / Cheng K-W / Wang TY / Poh Y-P / Liu H / Chou T-F / Nannenga B / Chiu P-L | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Molecular asymmetry of a photosynthetic supercomplex from green sulfur bacteria. Authors: Ryan Puskar / Chloe Du Truong / Kyle Swain / Saborni Chowdhury / Ka-Yi Chan / Shan Li / Kai-Wen Cheng / Ting Yu Wang / Yu-Ping Poh / Yuval Mazor / Haijun Liu / Tsui-Fen Chou / Brent L Nannenga / Po-Lin Chiu / ![]() Abstract: The photochemical reaction center (RC) features a dimeric architecture for charge separation across the membrane. In green sulfur bacteria (GSB), the trimeric Fenna-Matthews-Olson (FMO) complex ...The photochemical reaction center (RC) features a dimeric architecture for charge separation across the membrane. In green sulfur bacteria (GSB), the trimeric Fenna-Matthews-Olson (FMO) complex mediates the transfer of light energy from the chlorosome antenna complex to the RC. Here we determine the structure of the photosynthetic supercomplex from the GSB Chlorobaculum tepidum using single-particle cryogenic electron microscopy (cryo-EM) and identify the cytochrome c subunit (PscC), two accessory protein subunits (PscE and PscF), a second FMO trimeric complex, and a linker pigment between FMO and the RC core. The protein subunits that are assembled with the symmetric RC core generate an asymmetric photosynthetic supercomplex. One linker bacteriochlorophyll (BChl) is located in one of the two FMO-PscA interfaces, leading to differential efficiencies of the two energy transfer branches. The two FMO trimeric complexes establish two different binding interfaces with the RC cytoplasmic surface, driven by the associated accessory subunits. This structure of the GSB photosynthetic supercomplex provides mechanistic insight into the light excitation energy transfer routes and a possible evolutionary transition intermediate of the bacterial photosynthetic supercomplex from the primitive homodimeric RC. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 168.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 27 KB 27 KB | Display Display | ![]() |
Images | ![]() | 65 KB | ||
Filedesc metadata | ![]() | 7.8 KB | ||
Others | ![]() ![]() | 165.3 MB 165.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7ueaMC ![]() 7uebC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | RC-FMO1 map | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.04 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: RC-FMO1 half map 1
File | emd_26469_half_map_1.map | ||||||||||||
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Annotation | RC-FMO1 half map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: RC-FMO1 half map 2
File | emd_26469_half_map_2.map | ||||||||||||
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Annotation | RC-FMO1 half map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Photosynthetic assembly (RC-FMO1)
+Supramolecule #1: Photosynthetic assembly (RC-FMO1)
+Macromolecule #1: Photosystem P840 reaction center, large subunit
+Macromolecule #2: Photosystem P840 reaction center iron-sulfur protein
+Macromolecule #3: Cytochrome c
+Macromolecule #4: P840 reaction center 17 kDa protein
+Macromolecule #5: Bacteriochlorophyll a protein
+Macromolecule #6: Bacteriochlorophyll A isomer
+Macromolecule #7: BACTERIOCHLOROPHYLL A
+Macromolecule #8: [(2R,3S,4S,5R,6R)-6-[(10E,12E,14E)-2,6,10,14,19,23-hexamethyl-25-...
+Macromolecule #9: 2-[(1E,3E,5E,7E,9E,11E,13E,15E,17E,19E)-3,7,12,16,20,24-hexamethy...
+Macromolecule #10: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+Macromolecule #11: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+Macromolecule #12: CALCIUM ION
+Macromolecule #13: Chlorophyll A ester
+Macromolecule #14: IRON/SULFUR CLUSTER
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.1 mg/mL | ||||||||||||
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Buffer | pH: 8 Component:
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Grid | Model: C-flat-2/1 / Material: COPPER / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec. | ||||||||||||
Vitrification | Cryogen name: ETHANE / Instrument: HOMEMADE PLUNGER |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number real images: 32898 / Average exposure time: 6.0 sec. / Average electron dose: 45.4 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 70.0 µm / Calibrated magnification: 47259 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |