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Yorodumi- EMDB-25206: Cryo-EM structure of Torpedo acetylcholine receptor in complex wi... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-25206 | |||||||||
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Title | Cryo-EM structure of Torpedo acetylcholine receptor in complex with carbachol, desensitized state | |||||||||
Map data | Cryo-EM structure of Torpedo acetylcholine receptor in complex with carbachol, desensitized state | |||||||||
Sample |
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Function / homology | Function and homology information acetylcholine-gated monoatomic cation-selective channel activity / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / transmembrane signaling receptor activity / postsynaptic membrane / neuron projection Similarity search - Function | |||||||||
Biological species | Tetronarce californica (Pacific electric ray) / Pacific electric ray (Pacific electric ray) / Pacific electric ray, Torpedo californica | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.77 Å | |||||||||
Authors | Rahman MM / Basta T / Teng J / Lee M / Worrell BT / Stowell MHB / Hibbs RE | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Nat Struct Mol Biol / Year: 2022 Title: Structural mechanism of muscle nicotinic receptor desensitization and block by curare. Authors: Md Mahfuzur Rahman / Tamara Basta / Jinfeng Teng / Myeongseon Lee / Brady T Worrell / Michael H B Stowell / Ryan E Hibbs / Abstract: Binding of the neurotransmitter acetylcholine to its receptors on muscle fibers depolarizes the membrane and thereby triggers muscle contraction. We sought to understand at the level of three- ...Binding of the neurotransmitter acetylcholine to its receptors on muscle fibers depolarizes the membrane and thereby triggers muscle contraction. We sought to understand at the level of three-dimensional structure how agonists and antagonists alter nicotinic acetylcholine receptor conformation. We used the muscle-type receptor from the Torpedo ray to first define the structure of the receptor in a resting, activatable state. We then determined the receptor structure bound to the agonist carbachol, which stabilizes an asymmetric, closed channel desensitized state. We find conformational changes in a peripheral membrane helix are tied to recovery from desensitization. To probe mechanisms of antagonism, we obtained receptor structures with the active component of curare, a poison arrow toxin and precursor to modern muscle relaxants. d-Tubocurarine stabilizes the receptor in a desensitized-like state in the presence and absence of agonist. These findings define the transitions between resting and desensitized states and reveal divergent means by which antagonists block channel activity of the muscle-type nicotinic receptor. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_25206.map.gz | 5.8 MB | EMDB map data format | |
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Header (meta data) | emd-25206-v30.xml emd-25206.xml | 18.9 KB 18.9 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_25206_fsc.xml | 9.1 KB | Display | FSC data file |
Images | emd_25206.png | 90.1 KB | ||
Others | emd_25206_additional_1.map.gz | 4.9 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-25206 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-25206 | HTTPS FTP |
-Validation report
Summary document | emd_25206_validation.pdf.gz | 364.9 KB | Display | EMDB validaton report |
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Full document | emd_25206_full_validation.pdf.gz | 364.5 KB | Display | |
Data in XML | emd_25206_validation.xml.gz | 10.9 KB | Display | |
Data in CIF | emd_25206_validation.cif.gz | 14.3 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-25206 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-25206 | HTTPS FTP |
-Related structure data
Related structure data | 7smrMC 7smmC 7smqC 7smsC 7smtC C: citing same article (ref.) M: atomic model generated by this map |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_25206.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Cryo-EM structure of Torpedo acetylcholine receptor in complex with carbachol, desensitized state | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.07 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Additional map: Unsharpened map
File | emd_25206_additional_1.map | ||||||||||||
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Annotation | Unsharpened map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
+Entire : Torpedo acetylcholine receptor in complex with carbachol, desensi...
+Supramolecule #1: Torpedo acetylcholine receptor in complex with carbachol, desensi...
+Macromolecule #1: Acetylcholine receptor subunit alpha
+Macromolecule #2: Acetylcholine receptor subunit delta
+Macromolecule #3: Acetylcholine receptor subunit beta
+Macromolecule #4: Acetylcholine receptor subunit gamma
+Macromolecule #8: 2-[(AMINOCARBONYL)OXY]-N,N,N-TRIMETHYLETHANAMINIUM
+Macromolecule #9: CHOLESTEROL
+Macromolecule #10: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(tri...
+Macromolecule #11: 2-acetamido-2-deoxy-beta-D-glucopyranose
+Macromolecule #12: nonane
+Macromolecule #13: water
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 35.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |