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- EMDB-24191: structure of C2 projections and MIPs -

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基本情報

登録情報
データベース: EMDB / ID: EMD-24191
タイトルstructure of C2 projections and MIPs
マップデータcryo-EM map of C2 of Central Pair
試料
  • 複合体: Structure of C2 projections and MIPs
    • タンパク質・ペプチド: x 18種
  • リガンド: x 3種
キーワードcentral pair apparatus / C2 projection / kinesin motor protein / STRUCTURAL PROTEIN
機能・相同性
機能・相同性情報


axonemal central apparatus / organelle / cilium movement / axoneme assembly / motile cilium / microtubule motor activity / kinesin complex / microtubule-based movement / regulation of cytoskeleton organization / axoneme ...axonemal central apparatus / organelle / cilium movement / axoneme assembly / motile cilium / microtubule motor activity / kinesin complex / microtubule-based movement / regulation of cytoskeleton organization / axoneme / cilium assembly / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / structural constituent of cytoskeleton / microtubule cytoskeleton organization / mitotic cell cycle / microtubule binding / microtubule / hydrolase activity / GTPase activity / calcium ion binding / GTP binding / ATP hydrolysis activity / ATP binding / metal ion binding / cytoplasm
類似検索 - 分子機能
MYCBP-associated protein / Protein FAM228 / : / : / : / : / MYCBP-associated protein family / Flagellar-associated PapD-like / CH-like domain in sperm protein / CH-like domain in sperm protein ...MYCBP-associated protein / Protein FAM228 / : / : / : / : / MYCBP-associated protein family / Flagellar-associated PapD-like / CH-like domain in sperm protein / CH-like domain in sperm protein / : / : / : / Kinesin-like protein / Kinesin motor domain signature. / Kinesin motor domain, conserved site / Kinesin motor domain / Kinesin motor domain profile. / Kinesin motor, catalytic domain. ATPase. / Kinesin motor domain / Calponin homology domain / CH domain superfamily / Calponin homology (CH) domain profile. / Quinoprotein alcohol dehydrogenase-like superfamily / Kinesin motor domain superfamily / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Tetratricopeptide-like helical domain superfamily / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Immunoglobulin-like fold / P-loop containing nucleoside triphosphate hydrolase
類似検索 - ドメイン・相同性
Uncharacterized protein / Calponin-homology (CH) domain-containing protein / Cilia- and flagella-associated protein 70 / Abnormal spindle-like microcephaly-associated protein ASH domain-containing protein / Flagellar associated protein / Flagellar associated protein / EF-hand domain-containing protein / Kinesin-like protein / Flagellar associated protein / Tubulin beta-1/beta-2 chain ...Uncharacterized protein / Calponin-homology (CH) domain-containing protein / Cilia- and flagella-associated protein 70 / Abnormal spindle-like microcephaly-associated protein ASH domain-containing protein / Flagellar associated protein / Flagellar associated protein / EF-hand domain-containing protein / Kinesin-like protein / Flagellar associated protein / Tubulin beta-1/beta-2 chain / Tubulin alpha-1 chain / Flagellar WD repeat-containing protein Pf20
類似検索 - 構成要素
生物種Chlamydomonas reinhardtii (クラミドモナス)
手法単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.5 Å
データ登録者Han L / Zhang K
資金援助 米国, 1件
OrganizationGrant number国
Other private 米国
引用ジャーナル: Nat Struct Mol Biol / 年: 2022
タイトル: Cryo-EM structure of an active central apparatus.
著者: Long Han / Qinhui Rao / Renbin Yang / Yue Wang / Pengxin Chai / Yong Xiong / Kai Zhang /
要旨: Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating ...Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating regulator is the conserved central apparatus (CA) of motile cilia, composed of a pair of microtubules (C1 and C2) associated with hundreds of protein subunits per repeating unit. It is largely unclear how the CA plays its regulatory roles in ciliary motility. Here, we present high-resolution structures of Chlamydomonas reinhardtii CA by cryo-electron microscopy (cryo-EM) and its dynamic conformational behavior at multiple scales. The structures show how functionally related projection proteins of CA are clustered onto a spring-shaped scaffold of armadillo-repeat proteins, facilitated by elongated rachis-like proteins. The two halves of the CA are brought together by elastic chain-like bridge proteins to achieve coordinated activities. We captured an array of kinesin-like protein (KLP1) in two different stepping states, which are actively correlated with beating wave propagation of cilia. These findings establish a structural framework for understanding the role of the CA in cilia.
履歴
登録2021年6月7日-
ヘッダ(付随情報) 公開2022年5月18日-
マップ公開2022年5月18日-
更新2024年5月29日-
現状2024年5月29日処理サイト: RCSB / 状態: 公開

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構造の表示

添付画像

emd_24191.png

ダウンロードとリンク

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マップ

ファイルダウンロード / ファイル: emd_24191.map.gz / 形式: CCP4 / 大きさ: 639.6 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈cryo-EM map of C2 of Central Pair
ボクセルのサイズX=Y=Z: 1.33 Å
密度
表面レベル登録者による: 0.5
最小 - 最大0.0 - 9.615781999999999
平均 (標準偏差)0.046113964 (±0.13833103)
対称性空間群: 1
詳細

EMDB XML:

マップ形状
Axis orderXYZ
Origin000
サイズ526606526
Spacing606526526
セルA: 805.9794 Å / B: 699.57947 Å / C: 699.57947 Å
α=β=γ: 90.0 °

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添付データ

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追加マップ: C2 microtubule map with overall mask, GSFSC resolution 3.49 angstrom.

ファイルemd_24191_additional_1.map
注釈C2 microtubule map with overall mask, GSFSC resolution 3.49 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.

ファイルemd_24191_additional_10.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.08 angstrom.

ファイルemd_24191_additional_11.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.08 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.12 angstrom.

ファイルemd_24191_additional_12.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.12 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.

ファイルemd_24191_additional_13.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.16 angstrom.

ファイルemd_24191_additional_14.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.16 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.19 angstrom.

ファイルemd_24191_additional_15.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.19 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.07 angstrom.

ファイルemd_24191_additional_16.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.07 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.16 angstrom.

ファイルemd_24191_additional_17.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.16 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.04 angstrom.

ファイルemd_24191_additional_18.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.04 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.

ファイルemd_24191_additional_19.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.07 angstrom.

ファイルemd_24191_additional_2.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.07 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 2.99 angstrom.

ファイルemd_24191_additional_20.map
注釈C2 microtubule map with local mask, GSFSC resolution 2.99 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 2.93 angstrom.

ファイルemd_24191_additional_21.map
注釈C2 microtubule map with local mask, GSFSC resolution 2.93 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.04 angstrom.

ファイルemd_24191_additional_22.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.04 angstrom.
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追加マップ: KLP1 map with local mask, GSFSC resolution 3.41 angstrom.

ファイルemd_24191_additional_23.map
注釈KLP1 map with local mask, GSFSC resolution 3.41 angstrom.
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追加マップ: C2a arm map with overall mask, GSFSC resolution 4.41 angstrom.

ファイルemd_24191_additional_24.map
注釈C2a arm map with overall mask, GSFSC resolution 4.41 angstrom.
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追加マップ: C2a arm map with local mask, GSFSC resolution 3.89 angstrom.

ファイルemd_24191_additional_25.map
注釈C2a arm map with local mask, GSFSC resolution 3.89 angstrom.
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追加マップ: C2a arm map with local mask, GSFSC resolution 3.66 angstrom.

ファイルemd_24191_additional_26.map
注釈C2a arm map with local mask, GSFSC resolution 3.66 angstrom.
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追加マップ: C2a arm map with local mask, GSFSC resolution 3.63 angstrom.

ファイルemd_24191_additional_27.map
注釈C2a arm map with local mask, GSFSC resolution 3.63 angstrom.
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追加マップ: C2a arm map with local mask, GSFSC resolution 3.92 angstrom.

ファイルemd_24191_additional_28.map
注釈C2a arm map with local mask, GSFSC resolution 3.92 angstrom.
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追加マップ: C2a arm map with local mask, GSFSC resolution 3.92 angstrom.

ファイルemd_24191_additional_29.map
注釈C2a arm map with local mask, GSFSC resolution 3.92 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.05 angstrom.

ファイルemd_24191_additional_3.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.05 angstrom.
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追加マップ: C2a arm map with local mask, GSFSC resolution 3.87 angstrom.

ファイルemd_24191_additional_30.map
注釈C2a arm map with local mask, GSFSC resolution 3.87 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.01 angstrom.

ファイルemd_24191_additional_4.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.01 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 2.99 angstrom.

ファイルemd_24191_additional_5.map
注釈C2 microtubule map with local mask, GSFSC resolution 2.99 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.15 angstrom.

ファイルemd_24191_additional_6.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.15 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.09 angstrom.

ファイルemd_24191_additional_7.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.09 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.08 angstrom.

ファイルemd_24191_additional_8.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.08 angstrom.
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追加マップ: C2 microtubule map with local mask, GSFSC resolution 3.09 angstrom.

ファイルemd_24191_additional_9.map
注釈C2 microtubule map with local mask, GSFSC resolution 3.09 angstrom.
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試料の構成要素

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全体 : Structure of C2 projections and MIPs

全体名称: Structure of C2 projections and MIPs
要素
  • 複合体: Structure of C2 projections and MIPs
    • タンパク質・ペプチド: Flagellar WD repeat-containing protein Pf20
    • タンパク質・ペプチド: FAP178
    • タンパク質・ペプチド: FAP147
    • タンパク質・ペプチド: FAP239
    • タンパク質・ペプチド: FAP225
    • タンパク質・ペプチド: FAP213
    • タンパク質・ペプチド: FAP196
    • タンパク質・ペプチド: Unassigned protein-1
    • タンパク質・ペプチド: Unassigned protein-2
    • タンパク質・ペプチド: Unassigned protein-3
    • タンパク質・ペプチド: Unassigned protein-4
    • タンパク質・ペプチド: Kinesin-like protein
    • タンパク質・ペプチド: FAP65
    • タンパク質・ペプチド: FAP70
    • タンパク質・ペプチド: Unassigned protein-5
    • タンパク質・ペプチド: Unassigned protein-6
    • タンパク質・ペプチド: Tubulin beta
    • タンパク質・ペプチド: Tubulin alpha
  • リガンド: ADENOSINE-5'-DIPHOSPHATE
  • リガンド: GUANOSINE-5'-DIPHOSPHATE
  • リガンド: GUANOSINE-5'-TRIPHOSPHATE

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超分子 #1: Structure of C2 projections and MIPs

超分子名称: Structure of C2 projections and MIPs / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#18
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
株: CC124

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分子 #1: Flagellar WD repeat-containing protein Pf20

分子名称: Flagellar WD repeat-containing protein Pf20 / タイプ: protein_or_peptide / ID: 1 / コピー数: 4 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 65.929133 KDa
配列文字列: MAAAGLDTLR LEDSDDDFKY EEVDVMSDGE DDASEDLDAA LRKLQQFTSK QEAATGPART TEVKPGQVVK KPEVIDDFLR NFFIKMGLS RTCECFEAEW YELKATGRLD NSTTVPDVYL RNAELEDDVA GLRRELAEAK SIAGRASATW DKFRKERDFH R MHHKRVAQ ...文字列:
MAAAGLDTLR LEDSDDDFKY EEVDVMSDGE DDASEDLDAA LRKLQQFTSK QEAATGPART TEVKPGQVVK KPEVIDDFLR NFFIKMGLS RTCECFEAEW YELKATGRLD NSTTVPDVYL RNAELEDDVA GLRRELAEAK SIAGRASATW DKFRKERDFH R MHHKRVAQ EKNKLLTDLR RLKEHYAKYE PTILELKKKY ETLMKEKMMM SLERDKLAAR VDALEQVASS PLPGAERSLG GQ STAAAGG GASGRALGAT NRALTGDVPP AAGAAAAAAT GRSGAVSAGP RSGWASLNAP PRRNPYADLE FPAAPVKMLS LNK TFKGHL LSVANLALHP TKPILVTASD DKTWKMWHMP GGDLIMCGEG HKDWVAGVDF HPAGTCLASG GGDSAVKIWD FEKQ RCVTT FTDHKQAIWS VRFHHLGEVV ASGSLDHTVR LWDLPAGKCR MALRGHVDSV NDLAWQPFSS SLATASSDKT VSVWD ARAG LCTQTYYGHQ NSCNGVSFNI LGTQLASTDA DGVVKLWDTR MTAEVATINT GKHPANKSCF DRSGQVLAVA CDDGKV KAY STTDGVLQAE LAGHEDAVQA VLFDPAGQYL VSCGSDNTFR LWS

UniProtKB: Flagellar WD repeat-containing protein Pf20

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分子 #2: FAP178

分子名称: FAP178 / タイプ: protein_or_peptide / ID: 2 / コピー数: 4 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 23.511406 KDa
配列文字列: MTNRATLSQT ATSLTSTTGR RDRLQRQEDT TLFGYESPPD TPALHRDVLK WVQGLDLSQS LKNCRRDVAN GFLVAEIFSR YFPADIQMH SFANAASSHF KRDNWTQLQA FCGRQGINLP GDLVEGCVQG VHGAAIALLE HLYEAFTGKK VPRLKVPDAA A AAAAAAAA ...文字列:
MTNRATLSQT ATSLTSTTGR RDRLQRQEDT TLFGYESPPD TPALHRDVLK WVQGLDLSQS LKNCRRDVAN GFLVAEIFSR YFPADIQMH SFANAASSHF KRDNWTQLQA FCGRQGINLP GDLVEGCVQG VHGAAIALLE HLYEAFTGKK VPRLKVPDAA A AAAAAAAA RGGPGAVGGS GEGAKLISST VKTAQNLEFG AVTTQQLAGA DATALRRRLA GGTS

UniProtKB: Calponin-homology (CH) domain-containing protein

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分子 #3: FAP147

分子名称: FAP147 / タイプ: protein_or_peptide / ID: 3 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 102.141609 KDa
配列文字列: MVGPSEPAGL PPKAKLKAYK VLGDVGEFQE RLAATLPPPK APEPPNVFNI SDTSEFPPDV WFAATISPDN GVAVIASYRE LCKARALYQ SQLTNIHTAR RQYGMGGSMG PGAGAPLPLP PAQQAAAENR TIAETMRVAA DTRKLGKFEQ QQAKWDEVSS T LAYRVGRA ...文字列:
MVGPSEPAGL PPKAKLKAYK VLGDVGEFQE RLAATLPPPK APEPPNVFNI SDTSEFPPDV WFAATISPDN GVAVIASYRE LCKARALYQ SQLTNIHTAR RQYGMGGSMG PGAGAPLPLP PAQQAAAENR TIAETMRVAA DTRKLGKFEQ QQAKWDEVSS T LAYRVGRA PTELAMQRGP AWRTRAELTE LLYRAQPRDA RGSNPDEVWT ASLRDAWERI LPLGSIFSGL AIKIRDRPGE LP ATRAARV GRPLDPLLAP LGGGGTTLSP ATLGHPAAAA AHAQHVATLA ANGVMLGATT NKPPLGRSLS ARGRAWEDSE MLK QRVAEY GTRLRALAPH DPDFGALVVA GEALESQLEA LAGAPITLAE VESHVRGHAP EAFEAYKAVK DEMEAQLRAA LEAE EAQRA AQEAAAAAAA PQPGPHVAFS SPFVSLACHV GEKAHGSVTL VSRGTAAVNW SWRRVPAPQH AHAATELSQP PCFAA SLQS GVLLPGQSLT VAVTFEAAAA GTYREAWELV TRPPLQGSEG PCLTLRLRGA AEVRDESGTG RGALEEALAE KEKRAK VAA ALERVLRDVR MPRRPQPHES VEELAAGDAW DRVNGCAAGA VGGPYAWPPL FHSPGVQSEL AEVYREAEAA LAAALKP SV DEDPKKKKKE TSARKGKKGD EPPPPPPKYP PSWTEPAPAG AGCSLAVLDE LLTELAAAAP AAAAPLAVRA EEAKARAR V PPNNRKVLRR AMRVLVGKMV DAVEEKFNVI RTDLEAKSAA EAAAAAAAAA AAAGEDGDDA DGEASAISVG AVKEKDKEK EKDGKEKKGG AKEKPGAKGG AGKDKKGGSA SHTDDGAAPE PGAGLPNVEH LQPVFHDKAT TAAKAAIKAA LNTLLDEVHR HKVAAGDAL EANIRSLDSR IEAAQADAQA NQEPAAAEAA AAAGLALFAP AAGGVPAPGP GPAARARSAE LEQLYWERLC V LRTWTGLG IPTRADVR

UniProtKB: Flagellar associated protein

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分子 #4: FAP239

分子名称: FAP239 / タイプ: protein_or_peptide / ID: 4 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 57.734598 KDa
配列文字列: MPPQLGREVQ ERVKVYGPLN ELTYEGRLLT QTLQDELNRS ISAPAGPRSP WYEGDPELES MRERVRQQRA IREAQRRRDH AALTASIQK RNLQEEQRRD AMLGSLLGDV IGGLTDPNSP LAEAEAALSH ADKVRRKKKE SLHNEWSTQV FDTIQGRLQA A VDARDPAA ...文字列:
MPPQLGREVQ ERVKVYGPLN ELTYEGRLLT QTLQDELNRS ISAPAGPRSP WYEGDPELES MRERVRQQRA IREAQRRRDH AALTASIQK RNLQEEQRRD AMLGSLLGDV IGGLTDPNSP LAEAEAALSH ADKVRRKKKE SLHNEWSTQV FDTIQGRLQA A VDARDPAA IESRLKTQYD QYLHTTNTKV AVFRDVIIEQ DYNPLAAADA AIRVPTGDIR DPLKRDVLKG EYERRLMTGG RG GGGASPT GRGGAAAAGA GSIYGPLGKE TLGTQQWGEL AVKATPYGHC TDGQGGYVAR PLSGSAVALR ASRVPMDHYD YPV GNAAAA AEVPPGKRIV PGPEQRRGRQ DLFDVVQHTV HLKPQGYTGG DQWLEHKGKG NAPGPEQRRG RRDLADVLQQ KAVA DGPRG TSAPARGDQL QHKEQGDAWL DAKGKRRVEG PEMRRGRQGL YETLQQTSNP YQGGNKVGDA WLEHKGRKVQ PRPEP EAAA ALSAVPPLPT VRPPRVGDDK KYAVNIEAAM GQMTVKDGAK VTGW

UniProtKB: Flagellar associated protein

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分子 #5: FAP225

分子名称: FAP225 / タイプ: protein_or_peptide / ID: 5 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 81.430711 KDa
配列文字列: MATYEPPRSP GSRRVRRHAM GVSNASSIDE CEASSSARST VTLIQSGRLV RLQPHERPTD SVARETRTED RPIVDKVHDK LFKAHRERF VHKVLRSYAQ DDSGLLTPDQ LRSALDRLHT GLDAAEKDRI VARVAPAQHG KVHYMDFIRS LESPQPLGGP G LGVGFPGV ...文字列:
MATYEPPRSP GSRRVRRHAM GVSNASSIDE CEASSSARST VTLIQSGRLV RLQPHERPTD SVARETRTED RPIVDKVHDK LFKAHRERF VHKVLRSYAQ DDSGLLTPDQ LRSALDRLHT GLDAAEKDRI VARVAPAQHG KVHYMDFIRS LESPQPLGGP G LGVGFPGV TPQRAAAAGT APTFWNWQRH KKQHVPGLLE EVREGTFEQA QSDALLTSLM STKLNQYRDK LRLIFRQMDG NR NSLIDRE EFIRGIAKLR INVSKAQVER LFDLCDVDKS GELDYEEFVN RFEENGLASA ARNQTRAQPQ QPDGAPATVP LAQ SLGLTQ DEVAGALSHP MVHELARSLY GKASGATSVF VRNDLTRCGQ LPVRDMTRCC QALVPGISER QVAAVMAVVD PNSA GGVDY RAFVQKLTET GVANPRMLHS APARGEGFTA TGALTRFGEG GSLTAPDALP SAGSRSLSAV GTCRSPGGSG TTVLP ISAG LGVVLQPGGT LPPGAVTTRP AADRTSLDDL HDVCVAPFLE SLSRAGNNDL PAQHDNNNNN GGAGGPATSL PSVTRS IDM GTLSRSASLP NAHGGSPTRF GGAGGGGGFG GTAAGLSATG SKAPTHATGR FSRFWDRRYA DTSHITSVDP CSASYAP SE GTYVRKGWGS GDASSDFLTY QGADRDQRAR QRQAVAVRTT ARSEVEAKLS GLDDASGLDD GRLQVARATK QRYEERAE M YDRTRQQHEG GSCIFGRLPP FHEHQLEAAN TPARVFW

UniProtKB: EF-hand domain-containing protein

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分子 #6: FAP213

分子名称: FAP213 / タイプ: protein_or_peptide / ID: 6 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 22.262674 KDa
配列文字列: MEGAAGPSGF RNVEPLSRQE RAAARDKDLL EKSRLQARNR GGPLKQPENV VGNPVMPARN APAFCDEYDR FNRDVAGEMN AKKQQNLQK KEEVYAVKRA EQYHRERSNW ETQAQAAARE AARLEASRTT GTGAKRNQGS ESYNIISLNY NNSSGGQQLA A KDTAVKEA ...文字列:
MEGAAGPSGF RNVEPLSRQE RAAARDKDLL EKSRLQARNR GGPLKQPENV VGNPVMPARN APAFCDEYDR FNRDVAGEMN AKKQQNLQK KEEVYAVKRA EQYHRERSNW ETQAQAAARE AARLEASRTT GTGAKRNQGS ESYNIISLNY NNSSGGQQLA A KDTAVKEA RQARAVNLYS KSHSVSHNII TGEPIKFPTA GKE

UniProtKB: Flagellar associated protein

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分子 #7: FAP196

分子名称: FAP196 / タイプ: protein_or_peptide / ID: 7 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 64.880324 KDa
配列文字列: MPLVLRHIAG LNGQMFGAMR SIPNSRGPYN IAQISGVSVI LGNENNLAPQ FTLRGHDDVI TAFAMSHGGS MLATGQKGSN SDVIVWDVA ACAPKYKFQE HDVEVATVSF SADDRLLLSA SCFTDAKLYV WDMATGKIVA KHGLEPGKRI TCSAWSPALA N GRAYTCAT ...文字列:
MPLVLRHIAG LNGQMFGAMR SIPNSRGPYN IAQISGVSVI LGNENNLAPQ FTLRGHDDVI TAFAMSHGGS MLATGQKGSN SDVIVWDVA ACAPKYKFQE HDVEVATVSF SADDRLLLSA SCFTDAKLYV WDMATGKIVA KHGLEPGKRI TCSAWSPALA N GRAYTCAT AAGEELALWT VDPFTGMVSG QKVVLGTVRR EYTSLAFSAD GAWLYGGTTS ADVVTVNVQR ASVQMTHPVC SG GVGALLL SPDGGGACLV GGRDGSLSTF NAAGGAWREL RPFAKVPGCV TSLSLSADGG AFLVGTAQGG VYRLDRTSLQ LHT ISQAQQ GQLTAVAFAP GQPEAVATSS TDGSIYVWST ADYTLQCRVQ EPTQAGGALC CVLTRDAVIS GWGDGNIRCH ARAA GGATC AQMWMIPGAH ALAHSVGVTA LRLSNGGAFL LSGGMGGELR VWDLRSREMA AHMKMHNSRI VGAAVMADDK HVAAA SEDR TWSLWDVGSE RVRTTWRGQS MFRGMAVSAD KVSVVTVTLD RKIQMWDVRG PDPRWTKVEA HGKEVGCVAS DHRGAA FAT GGADQAVRVW DWRNGGEVGG AACHSAPVAA MAFSPDDAGL VSVALDGSTC FWQLT

UniProtKB: Uncharacterized protein

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分子 #8: Unassigned protein-1

分子名称: Unassigned protein-1 / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
株: CC124
分子量理論値: 14.400733 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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分子 #9: Unassigned protein-2

分子名称: Unassigned protein-2 / タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
株: CC124
分子量理論値: 14.485838 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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分子 #10: Unassigned protein-3

分子名称: Unassigned protein-3 / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
株: CC124
分子量理論値: 13.634798 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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分子 #11: Unassigned protein-4

分子名称: Unassigned protein-4 / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
株: CC124
分子量理論値: 3.337105 KDa
配列文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

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分子 #12: Kinesin-like protein

分子名称: Kinesin-like protein / タイプ: protein_or_peptide / ID: 12 / コピー数: 4 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 83.108039 KDa
配列文字列: MVKQAVKVFV RTRPTATSGS GLKLGPDGQS VSVNVPKDLS AGPVNNQQEQ FSFKFDGVLE NVSQEAAYTT LAHEVVDSLM AGYNGTIFA YGQTGAGKTF TMSGGGTAYA HRGLIPRAIH HVFREVDMRA DKMYRVHVSY LEIYNEQLYD LLGDTPGTSD A LAVLEDSN ...文字列:
MVKQAVKVFV RTRPTATSGS GLKLGPDGQS VSVNVPKDLS AGPVNNQQEQ FSFKFDGVLE NVSQEAAYTT LAHEVVDSLM AGYNGTIFA YGQTGAGKTF TMSGGGTAYA HRGLIPRAIH HVFREVDMRA DKMYRVHVSY LEIYNEQLYD LLGDTPGTSD A LAVLEDSN SNTYVRGLTL VPVRSEEEAL AQFFLGEQGR TTAGHVLNAE SSRSHTVFTI HVEMRTSDAA SERAVLSKLN LV DLAGSER TKKTGVTGQT LKEAQFINRS LSFLEQTVNA LSRKDTYVPF RQTKLTAVLR DALGGNCKTV MVANIWAEPS HNE ETLSTL RFASRVRTLT TDLALNESND PALLLRRYER QIKELKAELA MRDTLSGKGR VSYDDLTDDE LRELHATCRR FLHG EAEPE DLPADSMKRV RETFKALRAV HVAIKADMAT QMATLRRATE EGSGAAARGG DSAGPSGVGD VDLRATGGFT VGHAP LDAR PPVRSELGSP GAGASGAEAL GEPRSPGGGL HAQASSHTDA GSNWGDAGPL SSPGGTRLAG IFGVSGDRNA VFRRYK VDV GEGRELAASL KAASIALADT KASIRSLGAS VNDAKQRIDE LSSALALRRG ATPAGGDGEV LDSEAYALMQ ELKSAKS RY RTDFDSLKSA REELEPQIQA VAVARAGLLE AFDRWAAAQS DTTLKRMATA GRAMSGIAPG EEDEMDAGEQ FERMQIAR I SERDPDSLAF HTALKRTGAA VSRPATVATG GNAKAAAMAT RKMEHTQAVN RGLAR

UniProtKB: Kinesin-like protein

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分子 #13: FAP65

分子名称: FAP65 / タイプ: protein_or_peptide / ID: 13 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 233.221547 KDa
配列文字列: MPASNGADQG TAPGGTMAYG VLSREQRKQM FGLDCPEQLE WRHWNPGTEY VKQLVLKNVS TSVLKIRFKQ PTSKAFGMDF PEPFKLRPG MSQPLKVVFR PLKQQHYSDN VELFVGNVSC LVPVHAYTPV THIEVPPALD FGFTPTKETV TAQLPVRNTG D VRVDVLWK ...文字列:
MPASNGADQG TAPGGTMAYG VLSREQRKQM FGLDCPEQLE WRHWNPGTEY VKQLVLKNVS TSVLKIRFKQ PTSKAFGMDF PEPFKLRPG MSQPLKVVFR PLKQQHYSDN VELFVGNVSC LVPVHAYTPV THIEVPPALD FGFTPTKETV TAQLPVRNTG D VRVDVLWK LDAPFSISPL FASLAPGEVV AFTASFTPPE ACSYTASAAC QLESGAAAIC KISGIGKFPY LSVEQAGVDF GA VVVGQRV ERLVRFGNHS VVPAHFAVTH DEAGPDDGVF TVGPARGTLG PEEYSMLKLS YTPRATGTFS SETFHIGTAG GNR VALNLR GTAVAPLVTL STRAFNFGNV AAGATASRVL YIRNHSAVPV PYDFQVDPLD VFAISRTRGV LAPDSTAHVT ITFR PMAGL AANLWRRVTL LLKDAEPQGV DLVATAYTDK SRPPPLSQRH VERYMARVLS GGPAVEESQL DSKPPSAATG AGGLD GASE GGAGPLLAGG GFGSLLPPLG DAAEGGEGAA SKLLGPDGWS LLFGGQDPAA ALTVDSDALD FGSCSRLSAS EYRSVT VTN HTPAKLTAFV LVPEWADPGA APGDAPQRVF QVFPESADMR PYGQATFKVA FRPPRDAAFF SQQLALVAHT KSQRNFR LV AEHQVLPAWC VPLRATGNTF LHSNPEFGPK VELSSRAVAF PPCRPGERVH QTLLIANSGD TPVAFSFGAA GAALGPLF S AKPAAGVVPP QGHVLVALRF APTDTRPAAA NALVTFNGVA SNAVSVSLRG AAHTPRLTTD LPSSTLYFRP TCVGASSQR AVTLHNPSRV PVAFRWRLPA RLQGLVTVSP AAGALRGNES VQLQWSFTPA AQKLYEARAA CLVMSPPEAA NGNAAGATAS AASLFPGAG LATGIEWYEG AGATAALAGM GEEEAADAVL LSLVGEGTQG AVALEPPSLE LGDLRVGHPV RRPLLLQNCS D GVLRYSLE VGPAEDDQPP AGDATLVDFT AGLDSAAEGG VGGGAGGAGG AALECWVDEP EGALPARASK TVMVTLFPRY RK RYSLQVR CRSSTVAPLL TGPNRPPSGG AGPRPLDAGS EAAAAAAAAA AAAAAPLPPP VIAPLTASTT FPTLEVTDVY CEG LPKQLL WQLLGLNDLN HHLRTEVTAT ELRLRAAQDR GALTTEAASA AMRPFLMEFG THGLGGRPRV VHVEISNPTP LPAS WQLHS FDDPDGVELE NWVEPGRPRT EGERMRDLIA EYKLFEMRPR SGELEPGARC TVTIEFRPSV EGSFELPVFL HITDG KRLR LQLQAVTTPE PLQLLALPPP LRTFRLEPVA LGERAPPLQM YVLRNGGPAP LHWRLDTAPL AALAEASWGH PVLELV GPE EGDIEEGGVA AINWRFSPLE AKEYRVEVPV LLGDGGIEVI ELLGRGFAPP PAPPHGAAAV QLIGPGAATS PAAPTTA AE DDDTPAAALD SVGGDAEAEA ARAAAAADRD WITWRGLSSA PSAGMAGRLA LVDHDLVSLG VTPVRGLTRR IIVLTNKS R YPLAFDWDLG CLAPPPPPGL AATQRAAAAS AMMGLGGGSL LPASQLQLLA GRPLQGALAI SPAAGSLEPG ERLVCRVSL HAGVTPQVFE GEVRCHVRID DDAVAEAEAA AAAAAAAGAA AVAAELPFVE QVEEVIAEAP VRGPPAPPPA VAAAQRASRL RSRLPVHQY MTTAVRTRIE PLNAAFTATM EARTRRLADA TRPPSWPEPQ SISVTLRGRI LDERQLGALR YVPPHERAAA R AAVVAGAA WVPPAMVPFW EEEGRSTPAV SAQPSSQGGG GYGVGGYGGG YGSGGSQGGE ASVAPSGGDP GGYSPRGGMA DS WPSLEPG LGMEETVVGP PTVGLDLSLS LQHAGGAGGA PGGDGDSDSR PGTPSMTAAA HHHHHHPRHA GPAPGSGTAE VME VTFSGD QQPTGGPMPS PPSISGAPDP DSATPDDDFA TSDAAVNAAA AVRAAGGGMG SPQPVDPGGS AAAAASELAP PALP PAPPR GGDIEYDALV SAREVLAGLF AELMEDADVV RAVAFLPEHE VPSFAEVRAT PPPCYDPAVR AAAEAARAEA EARAA AEAA TKAAAEAEAV AARAAAMLGP KHEASSSSSS SSSSSSSSSS SSSSSSAASS PGASPTGKAP VVEAATPRLV SAVGGM APS QPALALRPAP STPPRTPPKP SQGAAFAIPA AAAQAATEPP ALEDELAADT ELARVLVAPE WRAFADFVLE SAVLGLM QE SAAGDWEMPE EEVGR

UniProtKB: Abnormal spindle-like microcephaly-associated protein ASH domain-containing protein

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分子 #14: FAP70

分子名称: FAP70 / タイプ: protein_or_peptide / ID: 14 / コピー数: 4 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 111.116258 KDa
配列文字列: MAPKKKEDKD APPPEDTNPY GVFVSVTMPS ATVVLDVQDD AAAQRETHIT LNIPGCHVPW ASAHTAPSEG GVYQYSVVKH FRRSGGQDG LLQLINGVLT VTVNDSATNA VLASATVDQL QGFAIGQNTW STEGLDLVPA AEVPEGVPKA RSARLAFASI A LQERPLPE ...文字列:
MAPKKKEDKD APPPEDTNPY GVFVSVTMPS ATVVLDVQDD AAAQRETHIT LNIPGCHVPW ASAHTAPSEG GVYQYSVVKH FRRSGGQDG LLQLINGVLT VTVNDSATNA VLASATVDQL QGFAIGQNTW STEGLDLVPA AEVPEGVPKA RSARLAFASI A LQERPLPE GADAAALAAA PPPPEAELPP LLPYSYVSGE AAEKGNMVEL VVSGLSPLPP NLQAAADAAG GKLHVTVGLA LP GGGPAVA VSLAVAGGRV AAPAFRRELL PPGCLQALQY ALEDGLPVVL EVARYVSAEG MADPAFEGYH AAAAAPALAA GLG AAGATE AAAEGLSLTA WAASGAKTCL PPYTAPAGKP KPVEAEVPPA GTCLWEKAGA QLAVSLRFAR PVVPAWRPPP PPPR PLLEL IPPRDLTPKP PPTTAVDEFK AKVRVIARAL AEEYKAVLPP PDASVAAAAA GGGAEGRHKA LIFELNRSGK YAQMR DSLK TAVVSLVREK YRKSGSMSPN EMALLYNDLY GSLLAAVHSS LNDLVDAAAA RPRAPPPAPV PDKQRLGELL ELAAQA EAM GDTDRAELLH QRRLLAKNDA QVWYEYGTYC LRRGGAKRGR AEECFREALA LEPAHRGALL ALLGCSVAAG RNTDPAY LE SAEAAAHRLL DVAGRSSLDA WAALAVVYRA YGEAKRAELA SCEQEMARLE KQQLAAAAAA ASAGVSPSPS YGEGRAGG N GGGVPATEPS TASAGSVAGS AGELQARSFI SLANTLLESL ALPAEAALAL ELAAGLRHWP SVGPDTRTLH ALAGALAEQ ALARAAGGGA ASAAAEAMLT PGSSVLSMMR ADAGEAVSSV AAEAAWRCRL LVAQLHKARG ATDEAIRFYQ EYIEAARSSG RLAEVPLSA WLELAEAYAA RGQARFAADV FLLGASARPG CAVLWRGAGR CFVGAEELGP ADMALSEANV LDPEDPEAWG W LALVALRE GRAEDAEKAL AFGLRCGLGD PGLLLDIAAE YRAAGQRRAE QRVLQEVAVK LMPESCSARL LLARCLVAQR CG AEAAEAV AAARQLAAHE DDEAAVAELE AELRGMA

UniProtKB: Cilia- and flagella-associated protein 70

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分子 #15: Unassigned protein-5

分子名称: Unassigned protein-5 / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
株: CC124
分子量理論値: 7.507245 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

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分子 #16: Unassigned protein-6

分子名称: Unassigned protein-6 / タイプ: protein_or_peptide / ID: 16 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
株: CC124
分子量理論値: 14.826253 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

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分子 #17: Tubulin beta

分子名称: Tubulin beta / タイプ: protein_or_peptide / ID: 17 / コピー数: 52 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 49.665809 KDa
配列文字列: MREIVHIQGG QCGNQIGAKF WEVVSDEHGI DPTGTYHGDS DLQLERINVY FNEATGGRYV PRAILMDLEP GTMDSVRSGP YGQIFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAESCDC LQGFQVCHSL GGGTGSGMGT LLISKIREEY P DRMMLTFS ...文字列:
MREIVHIQGG QCGNQIGAKF WEVVSDEHGI DPTGTYHGDS DLQLERINVY FNEATGGRYV PRAILMDLEP GTMDSVRSGP YGQIFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAESCDC LQGFQVCHSL GGGTGSGMGT LLISKIREEY P DRMMLTFS VVPSPKVSDT VVEPYNATLS VHQLVENADE CMVLDNEALY DICFRTLKLT TPTFGDLNHL ISAVMSGITC CL RFPGQLN ADLRKLAVNL IPFPRLHFFM VGFTPLTSRG SQQYRALTVP ELTQQMWDAK NMMCAADPRH GRYLTASALF RGR MSTKEV DEQMLNVQNK NSSYFVEWIP NNVKSSVCDI PPKGLKMSAT FIGNSTAIQE MFKRVSEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDASAEEEGE FEGEEEEA

UniProtKB: Tubulin beta-1/beta-2 chain

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分子 #18: Tubulin alpha

分子名称: Tubulin alpha / タイプ: protein_or_peptide / ID: 18 / コピー数: 52 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 49.638008 KDa
配列文字列: MREVISIHIG QAGIQVGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRCIFLDL EPTVVDEVRT GTYRQLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLAL DRIRKLADNC TGLQGFLVFN AVGGGTGSGL GSLLLERLSV D YGKKSKLG ...文字列:
MREVISIHIG QAGIQVGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRCIFLDL EPTVVDEVRT GTYRQLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLAL DRIRKLADNC TGLQGFLVFN AVGGGTGSGL GSLLLERLSV D YGKKSKLG FTVYPSPQVS TAVVEPYNSV LSTHSLLEHT DVAVMLDNEA IYDICRRSLD IERPTYTNLN RLIAQVISSL TA SLRFDGA LNVDITEFQT NLVPYPRIHF MLSSYAPIIS AEKAYHEQLS VAEITNAAFE PASMMVKCDP RHGKYMACCL MYR GDVVPK DVNASVATIK TKRTIQFVDW CPTGFKCGIN YQPPTVVPGG DLAKVQRAVC MISNSTAIGE IFSRLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDFEEV GAESAEGAGE GEGEEY

UniProtKB: Tubulin alpha-1 chain

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分子 #19: ADENOSINE-5'-DIPHOSPHATE

分子名称: ADENOSINE-5'-DIPHOSPHATE / タイプ: ligand / ID: 19 / コピー数: 4 / 式: ADP
分子量理論値: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子*YM

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分子 #20: GUANOSINE-5'-DIPHOSPHATE

分子名称: GUANOSINE-5'-DIPHOSPHATE / タイプ: ligand / ID: 20 / コピー数: 52 / 式: GDP
分子量理論値: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子*YM

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分子 #21: GUANOSINE-5'-TRIPHOSPHATE

分子名称: GUANOSINE-5'-TRIPHOSPHATE / タイプ: ligand / ID: 21 / コピー数: 52 / 式: GTP
分子量理論値: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子*YM

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実験情報

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構造解析

手法クライオ電子顕微鏡法
解析単粒子再構成法
試料の集合状態filament

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試料調製

緩衝液pH: 7.4
凍結凍結剤: ETHANE

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電子顕微鏡法

顕微鏡FEI TITAN KRIOS
撮影フィルム・検出器のモデル: GATAN K2 QUANTUM (4k x 4k)
平均電子線量: 38.6 e/Å2
電子線加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
電子光学系C2レンズ絞り径: 100.0 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス（公称値）: 2.5 µm / 最小 デフォーカス（公称値）: 1.2 µm
実験機器
モデル: Titan Krios / 画像提供: FEI Company

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画像解析

初期モデルモデルのタイプ: NONE
最終 再構成解像度のタイプ: BY AUTHOR / 解像度: 3.5 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 192253
初期 角度割当タイプ: MAXIMUM LIKELIHOOD
最終 角度割当タイプ: MAXIMUM LIKELIHOOD

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbjlvh1.pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る