+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-24191 | |||||||||
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Title | structure of C2 projections and MIPs | |||||||||
Map data | cryo-EM map of C2 of Central Pair | |||||||||
Sample |
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Keywords | central pair apparatus / C2 projection / kinesin motor protein / STRUCTURAL PROTEIN | |||||||||
Function / homology | Function and homology information organelle / cilium movement / motile cilium / microtubule motor activity / kinesin complex / microtubule-based movement / regulation of cytoskeleton organization / axoneme / microtubule-based process / cilium assembly ...organelle / cilium movement / motile cilium / microtubule motor activity / kinesin complex / microtubule-based movement / regulation of cytoskeleton organization / axoneme / microtubule-based process / cilium assembly / structural constituent of cytoskeleton / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule binding / microtubule / hydrolase activity / GTPase activity / calcium ion binding / GTP binding / ATP hydrolysis activity / ATP binding / metal ion binding / cytoplasm Similarity search - Function | |||||||||
Biological species | Chlamydomonas reinhardtii (plant) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.5 Å | |||||||||
Authors | Han L / Zhang K | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Nat Struct Mol Biol / Year: 2022 Title: Cryo-EM structure of an active central apparatus. Authors: Long Han / Qinhui Rao / Renbin Yang / Yue Wang / Pengxin Chai / Yong Xiong / Kai Zhang / Abstract: Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating ...Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating regulator is the conserved central apparatus (CA) of motile cilia, composed of a pair of microtubules (C1 and C2) associated with hundreds of protein subunits per repeating unit. It is largely unclear how the CA plays its regulatory roles in ciliary motility. Here, we present high-resolution structures of Chlamydomonas reinhardtii CA by cryo-electron microscopy (cryo-EM) and its dynamic conformational behavior at multiple scales. The structures show how functionally related projection proteins of CA are clustered onto a spring-shaped scaffold of armadillo-repeat proteins, facilitated by elongated rachis-like proteins. The two halves of the CA are brought together by elastic chain-like bridge proteins to achieve coordinated activities. We captured an array of kinesin-like protein (KLP1) in two different stepping states, which are actively correlated with beating wave propagation of cilia. These findings establish a structural framework for understanding the role of the CA in cilia. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
-Validation report
Summary document | emd_24191_validation.pdf.gz | 419.9 KB | Display | EMDB validaton report |
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Full document | emd_24191_full_validation.pdf.gz | 419.5 KB | Display | |
Data in XML | emd_24191_validation.xml.gz | 4.5 KB | Display | |
Data in CIF | emd_24191_validation.cif.gz | 5.1 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-24191 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-24191 | HTTPS FTP |
-Related structure data
Related structure data | 7n61MC 7n6gC C: citing same article (ref.) M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_24191.map.gz / Format: CCP4 / Size: 639.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | cryo-EM map of C2 of Central Pair | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. generated in cubic-lattice coordinate | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.33 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
+Additional map: C2 microtubule map with overall mask, GSFSC resolution 3.49 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.08 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.12 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.16 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.19 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.07 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.16 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.04 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.07 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 2.99 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 2.93 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.04 angstrom.
+Additional map: KLP1 map with local mask, GSFSC resolution 3.41 angstrom.
+Additional map: C2a arm map with overall mask, GSFSC resolution 4.41 angstrom.
+Additional map: C2a arm map with local mask, GSFSC resolution 3.89 angstrom.
+Additional map: C2a arm map with local mask, GSFSC resolution 3.66 angstrom.
+Additional map: C2a arm map with local mask, GSFSC resolution 3.63 angstrom.
+Additional map: C2a arm map with local mask, GSFSC resolution 3.92 angstrom.
+Additional map: C2a arm map with local mask, GSFSC resolution 3.92 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.05 angstrom.
+Additional map: C2a arm map with local mask, GSFSC resolution 3.87 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.01 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 2.99 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.15 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.09 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.08 angstrom.
+Additional map: C2 microtubule map with local mask, GSFSC resolution 3.09 angstrom.
-Sample components
+Entire : Structure of C2 projections and MIPs
+Supramolecule #1: Structure of C2 projections and MIPs
+Macromolecule #1: Flagellar WD repeat-containing protein Pf20
+Macromolecule #2: FAP178
+Macromolecule #3: FAP147
+Macromolecule #4: FAP239
+Macromolecule #5: FAP225
+Macromolecule #6: FAP213
+Macromolecule #7: FAP196
+Macromolecule #8: Unassigned protein-1
+Macromolecule #9: Unassigned protein-2
+Macromolecule #10: Unassigned protein-3
+Macromolecule #11: Unassigned protein-4
+Macromolecule #12: Kinesin-like protein
+Macromolecule #13: FAP65
+Macromolecule #14: FAP70
+Macromolecule #15: Unassigned protein-5
+Macromolecule #16: Unassigned protein-6
+Macromolecule #17: Tubulin beta
+Macromolecule #18: Tubulin alpha
+Macromolecule #19: ADENOSINE-5'-DIPHOSPHATE
+Macromolecule #20: GUANOSINE-5'-DIPHOSPHATE
+Macromolecule #21: GUANOSINE-5'-TRIPHOSPHATE
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | filament |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 38.6 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | C2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 192253 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |