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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1ian | ||||||
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タイトル | HUMAN P38 MAP KINASE INHIBITOR COMPLEX | ||||||
![]() | P38 MAP KINASE | ||||||
![]() | SERINE/THREONINE-PROTEIN KINASE / PROTEIN SER/THR-KINASE / SERINE-THREONINE-PROTEIN KINASE complex | ||||||
機能・相同性 | ![]() positive regulation of cyclase activity / stress-activated protein kinase signaling cascade / Activation of PPARGC1A (PGC-1alpha) by phosphorylation / CD163 mediating an anti-inflammatory response / regulation of synaptic membrane adhesion / stress-induced premature senescence / cell surface receptor protein serine/threonine kinase signaling pathway / 3'-UTR-mediated mRNA stabilization / KSRP (KHSRP) binds and destabilizes mRNA / cartilage condensation ...positive regulation of cyclase activity / stress-activated protein kinase signaling cascade / Activation of PPARGC1A (PGC-1alpha) by phosphorylation / CD163 mediating an anti-inflammatory response / regulation of synaptic membrane adhesion / stress-induced premature senescence / cell surface receptor protein serine/threonine kinase signaling pathway / 3'-UTR-mediated mRNA stabilization / KSRP (KHSRP) binds and destabilizes mRNA / cartilage condensation / DSCAM interactions / cellular response to UV-B / Platelet sensitization by LDL / mitogen-activated protein kinase p38 binding / positive regulation of myoblast fusion / positive regulation of muscle cell differentiation / negative regulation of hippo signaling / positive regulation of myotube differentiation / NFAT protein binding / Myogenesis / Activation of the AP-1 family of transcription factors / ERK/MAPK targets / glucose import / regulation of cytokine production involved in inflammatory response / p38MAPK cascade / fatty acid oxidation / cellular response to lipoteichoic acid / MAP kinase kinase activity / response to dietary excess / response to muramyl dipeptide / RHO GTPases Activate NADPH Oxidases / MAP kinase activity / regulation of ossification / mitogen-activated protein kinase / cellular response to vascular endothelial growth factor stimulus / signal transduction in response to DNA damage / positive regulation of myoblast differentiation / chondrocyte differentiation / vascular endothelial growth factor receptor signaling pathway / stress-activated MAPK cascade / skeletal muscle tissue development / lipopolysaccharide-mediated signaling pathway / positive regulation of cardiac muscle cell proliferation / negative regulation of inflammatory response to antigenic stimulus / p38MAPK events / striated muscle cell differentiation / response to muscle stretch / positive regulation of interleukin-12 production / positive regulation of brown fat cell differentiation / osteoclast differentiation / positive regulation of erythrocyte differentiation / DNA damage checkpoint signaling / activated TAK1 mediates p38 MAPK activation / cellular response to ionizing radiation / stem cell differentiation / positive regulation of glucose import / NOD1/2 Signaling Pathway / bone development / response to insulin / placenta development / negative regulation of canonical Wnt signaling pathway / cell morphogenesis / platelet activation / cellular response to virus / VEGFA-VEGFR2 Pathway / spindle pole / positive regulation of protein import into nucleus / osteoblast differentiation / ADP signalling through P2Y purinoceptor 1 / glucose metabolic process / chemotaxis / positive regulation of reactive oxygen species metabolic process / cellular senescence / cellular response to tumor necrosis factor / cellular response to lipopolysaccharide / peptidyl-serine phosphorylation / protein phosphatase binding / angiogenesis / Oxidative Stress Induced Senescence / secretory granule lumen / Regulation of TP53 Activity through Phosphorylation / ficolin-1-rich granule lumen / transcription by RNA polymerase II / cell surface receptor signaling pathway / intracellular signal transduction / nuclear speck / protein serine kinase activity / protein serine/threonine kinase activity / glutamatergic synapse / apoptotic process / Neutrophil degranulation / positive regulation of gene expression / regulation of transcription by RNA polymerase II / enzyme binding / signal transduction / positive regulation of transcription by RNA polymerase II / mitochondrion / extracellular region / nucleoplasm / ATP binding 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Tong, L. | ||||||
![]() | ![]() タイトル: A highly specific inhibitor of human p38 MAP kinase binds in the ATP pocket. 著者: Tong, L. / Pav, S. / White, D.M. / Rogers, S. / Crane, K.M. / Cywin, C.L. / Brown, M.L. / Pargellis, C.A. #1: ![]() タイトル: Sb 203580 is a Specific Inhibitor of a Map Kinase Homologue which is Stimulated by Cellular Stresses and Interleukin-1 著者: Cuenda, A. / Rouse, J. / Doza, Y.N. / Meier, R. / Cohen, P. / Gallagher, T.F. / Young, P.R. / Lee, J.C. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 27 KB | 表示 | ![]() |
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PDB形式 | ![]() | 13.9 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 494.5 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 498.6 KB | 表示 | |
XML形式データ | ![]() | 2 KB | 表示 | |
CIF形式データ | ![]() | 5.6 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 42105.004 Da / 分子数: 1 / 変異: N-TERMINAL HIS-TAG / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() 参照: UniProt: Q16539, 転移酵素; リンを含む基を移すもの; キナーゼ(アルコールにつなげるもの) |
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#2: 化合物 |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.4 Å3/Da / 溶媒含有率: 45 % | |||||||||||||||||||||||||
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結晶化 | pH: 7.4 / 詳細: pH 7.4 | |||||||||||||||||||||||||
結晶化 | *PLUS 温度: 21-25 ℃ / 手法: 蒸気拡散法 / 詳細: Pav, S., (1997) Protein Sci., 6, 242. | |||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: FUJI / 検出器: IMAGE PLATE / 日付: 1996年8月31日 / 詳細: BENT MIRROR |
放射 | モノクロメーター: SI(111) / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.9792 Å / 相対比: 1 |
反射 | 解像度: 2→30 Å / Num. obs: 25453 / % possible obs: 99 % / Observed criterion σ(I): 1 / 冗長度: 7 % / Rmerge(I) obs: 0.046 / Rsym value: 0.046 / Net I/σ(I): 23 |
反射 シェル | 解像度: 2→2.1 Å / 冗長度: 5 % / Rmerge(I) obs: 0.157 / Mean I/σ(I) obs: 7 / Rsym value: 0.157 / % possible all: 95 |
反射 | *PLUS Num. measured all: 179632 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: SE-MET MAD, ![]() 開始モデル: ERK2 解像度: 2→6 Å / Data cutoff high absF: 10000000 / Data cutoff low absF: 1 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 2
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原子変位パラメータ | Biso mean: 28 Å2
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精密化ステップ | サイクル: LAST / 解像度: 2→6 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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