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Open data
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Basic information
Entry | Database: PDB / ID: 1av5 | ||||||
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Title | PKCI-SUBSTRATE ANALOG | ||||||
![]() | PROTEIN KINASE C INTERACTING PROTEIN | ||||||
![]() | ![]() ![]() | ||||||
Function / homology | ![]() purine ribonucleotide catabolic process / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Lima, C.D. / Klein, M.G. / Hendrickson, W.A. | ||||||
![]() | ![]() Title: Structure-based analysis of catalysis and substrate definition in the HIT protein family. Authors: Lima, C.D. / Klein, M.G. / Hendrickson, W.A. #1: ![]() Title: Three-Dimensional Structure of Human Protein Kinase C Interacting Protein 1, a Member of the Hit Family of Proteins Authors: Lima, C.D. / Klein, M.G. / Weinstein, I.B. / Hendrickson, W.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 59.1 KB | Display | ![]() |
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PDB format | ![]() | 43.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 13718.772 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-AP2 / | #3: Water | ChemComp-HOH / | ![]() Nonpolymer details | THE SUBSTRATE ANALOG (AMPCP) WAS SOAKED INTO THE CRYSTAL AND FOUND TO BIND IN THE ACTIVE SITE OF ...THE SUBSTRATE ANALOG (AMPCP) WAS SOAKED INTO THE CRYSTAL AND FOUND TO BIND IN THE ACTIVE SITE OF CHAIN B. THE ACTIVE SITE OF CHAIN A IS BLOCKED BY A LATTICE CONTACT AND IS NOT AVAILABLE TO THE SUBSTRATE. THE AMPCP STRUCTURE REPORTED HERE REPRESENTS | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.52 % | ||||||||||||||||||||
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Crystal grow![]() | pH: 6.5 / Details: GROWN FROM PEG8K PH6.5 | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion, hanging dropDetails: Lima, C.D., (1996) Proc.Nat.Acad.Sci.USA, 93, 5357. | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Oct 17, 1996 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→20 Å / Num. obs: 14358 / % possible obs: 71.5 % / Observed criterion σ(I): 2 / Redundancy: 4 % / Rmerge(I) obs: 0.056 |
Reflection | *PLUS Num. obs: 26213 / Num. measured all: 194693 |
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Processing
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Refinement | Resolution: 2→8 Å / Data cutoff high absF: 100000 / Data cutoff low absF: 0.1 / σ(F): 2 Details: REFINED ADENOSINE ALPHA/BETA-METHYLENE DIPHOSPHATE AS ADP.
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Displacement parameters | Biso mean: 31 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.31 Å / Luzzati d res low obs: 5 Å / Luzzati sigma a obs: 0.39 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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