maintenance of CRISPR repeat elements / endonuclease activity / defense response to virus / Hydrolases; Acting on ester bonds / DNA binding / RNA binding / metal ion binding Similarity search - Function
Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
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Electron microscopy
Microscope
TFS GLACIOS
Image recording
Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: COUNTING / Number grids imaged: 1 / Number real images: 1744 / Average exposure time: 46.33 sec. / Average electron dose: 30.0 e/Å2
Electron beam
Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron optics
Illumination mode: OTHER / Imaging mode: OTHER / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 92000
Sample stage
Cooling holder cryogen: NITROGEN
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Image processing
Particle selection
Number selected: 894269 / Details: Blob picking in cryoSPARC live session
Startup model
Type of model: INSILICO MODEL / In silico model: ab-initio cryoSPARC
Final reconstruction
Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.24 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.1.1) / Number images used: 119790
Initial angle assignment
Type: OTHER
Final angle assignment
Type: OTHER
FSC plot (resolution estimation)
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Atomic model buiding 1
Initial model
Chain - Source name: AlphaFold / Chain - Initial model type: in silico model
Output model
PDB-8pj9: Cas9 bound to cognate DNA, Streptococcus thermophilus DGCC 7710 CRISPR3 system
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