+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-17549 | |||||||||
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Title | Yeast 60S ribosomal subunit, RPL39 deletion | |||||||||
Map data | ||||||||||
Sample |
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Keywords | 60S ribosomal subunit / protein exit tunnel / RPL39 / RIBOSOME | |||||||||
Function / homology | Function and homology information hexon binding / pre-mRNA 5'-splice site binding / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / negative regulation of mRNA splicing, via spliceosome ...hexon binding / pre-mRNA 5'-splice site binding / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / negative regulation of mRNA splicing, via spliceosome / preribosome, large subunit precursor / L13a-mediated translational silencing of Ceruloplasmin expression / translational elongation / ribosomal large subunit export from nucleus / 90S preribosome / regulation of translational fidelity / protein-RNA complex assembly / translational termination / maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / translational initiation / macroautophagy / maintenance of translational fidelity / modification-dependent protein catabolic process / protein tag activity / rRNA processing / ribosome biogenesis / viral capsid / 5S rRNA binding / large ribosomal subunit rRNA binding / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / translation / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / host cell nucleus / nucleolus / RNA binding / nucleus / metal ion binding / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.66 Å | |||||||||
Authors | Rabl J / Banerjee A / Boehringer D / Zavolan M | |||||||||
Funding support | Switzerland, 1 items
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Citation | Journal: To Be Published Title: Yeast 60S ribosomal subunit, RPL39 deletion Authors: Rabl J / Banerjee A / Boehringer D / Zavolan M | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_17549.map.gz | 229.3 MB | EMDB map data format | |
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Header (meta data) | emd-17549-v30.xml emd-17549.xml | 61.7 KB 61.7 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_17549_fsc.xml | 13.1 KB | Display | FSC data file |
Images | emd_17549.png | 147.6 KB | ||
Masks | emd_17549_msk_1.map | 244.1 MB | Mask map | |
Filedesc metadata | emd-17549.cif.gz | 12.9 KB | ||
Others | emd_17549_additional_1.map.gz emd_17549_half_map_1.map.gz emd_17549_half_map_2.map.gz | 123.6 MB 226.7 MB 226.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-17549 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-17549 | HTTPS FTP |
-Validation report
Summary document | emd_17549_validation.pdf.gz | 1.1 MB | Display | EMDB validaton report |
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Full document | emd_17549_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | emd_17549_validation.xml.gz | 21.4 KB | Display | |
Data in CIF | emd_17549_validation.cif.gz | 27.7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-17549 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-17549 | HTTPS FTP |
-Related structure data
Related structure data | 8p8mMC 8p8nC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_17549.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.26 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_17549_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Additional map: #1
File | emd_17549_additional_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_17549_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_17549_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
+Entire : Yeast 60S ribosomal subunit without ribosomal protein RPL39
+Supramolecule #1: Yeast 60S ribosomal subunit without ribosomal protein RPL39
+Macromolecule #1: 25S rRNA
+Macromolecule #11: 5.8S rRNA
+Macromolecule #13: 5S rRNA
+Macromolecule #2: 60S ribosomal protein L26-A
+Macromolecule #3: 60S ribosomal protein L13-A
+Macromolecule #4: 60S ribosomal protein L27-A
+Macromolecule #5: 60S ribosomal protein L14-A
+Macromolecule #6: 60S ribosomal protein L28
+Macromolecule #7: 60S ribosomal protein L15-A
+Macromolecule #8: 60S ribosomal protein L29
+Macromolecule #9: 60S ribosomal protein L16-A
+Macromolecule #10: 60S ribosomal protein L30
+Macromolecule #12: 60S ribosomal protein L31-A
+Macromolecule #14: 60S ribosomal protein L32
+Macromolecule #15: 60S ribosomal protein L2-A
+Macromolecule #16: 60S ribosomal protein L33-A
+Macromolecule #17: 60S ribosomal protein L3
+Macromolecule #18: 60S ribosomal protein L34-A
+Macromolecule #19: 60S ribosomal protein L4-A
+Macromolecule #20: 60S ribosomal protein L35-A
+Macromolecule #21: 60S ribosomal protein L5
+Macromolecule #22: 60S ribosomal protein L36-A
+Macromolecule #23: 60S ribosomal protein L6-A
+Macromolecule #24: 60S ribosomal protein L37-A
+Macromolecule #25: 60S ribosomal protein L7-A
+Macromolecule #26: 60S ribosomal protein L38
+Macromolecule #27: 60S ribosomal protein L8-A
+Macromolecule #28: Ubiquitin-60S ribosomal protein L40
+Macromolecule #29: 60S ribosomal protein L9-A
+Macromolecule #30: 60S ribosomal protein L42-A
+Macromolecule #31: 60S ribosomal protein L10
+Macromolecule #32: 60S ribosomal protein L43-A
+Macromolecule #33: 60S ribosomal protein L11-A
+Macromolecule #34: 60S acidic ribosomal protein P0
+Macromolecule #35: 60S ribosomal protein L17-A
+Macromolecule #36: 60S ribosomal protein L18-A
+Macromolecule #37: 60S ribosomal protein L19-A
+Macromolecule #38: 60S ribosomal protein L20-A
+Macromolecule #39: 60S ribosomal protein L21-A
+Macromolecule #40: 60S ribosomal protein L22-A
+Macromolecule #41: 60S ribosomal protein L23-A
+Macromolecule #42: 60S ribosomal protein L24-A
+Macromolecule #43: 60S ribosomal protein L25
+Macromolecule #44: MAGNESIUM ION
+Macromolecule #45: SPERMINE
+Macromolecule #46: SPERMIDINE
+Macromolecule #47: ZINC ION
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.6 |
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Vitrification | Cryogen name: ETHANE-PROPANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 45.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
+Image processing
-Atomic model buiding 1
Refinement | Space: REAL / Protocol: FLEXIBLE FIT |
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Output model | PDB-8p8m: |