- EMDB-17209: Human Complement C3b in complex with Trypanosoma brucei ISG65. -
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基本情報
登録情報
データベース: EMDB / ID: EMD-17209
タイトル
Human Complement C3b in complex with Trypanosoma brucei ISG65.
マップデータ
Human Complement C3 in complex with T. brucei ISG65. Composite map from local alignments of the CUB-TED-ISG65 and remaining C3b regions. Postprocessed in DeepEMhancer.
試料
複合体: Complex of Human Complement C3b and T. brucei ISG65
複合体: Human Complement C3b
タンパク質・ペプチド: Complement C3f fragment
タンパク質・ペプチド: Complement C3
複合体: Trypanosoma brucei ISG65
タンパク質・ペプチド: ISG65 G
キーワード
Complement system / parasite virulence / trypanosome surface protein / host-pathogen complex / IMMUNE SYSTEM
機能・相同性
機能・相同性情報
oviduct epithelium development / C5L2 anaphylatoxin chemotactic receptor binding / regulation of triglyceride biosynthetic process / positive regulation of activation of membrane attack complex / vertebrate eye-specific patterning / positive regulation of apoptotic cell clearance / complement-mediated synapse pruning / Alternative complement activation / positive regulation of G protein-coupled receptor signaling pathway / positive regulation of lipid storage ...oviduct epithelium development / C5L2 anaphylatoxin chemotactic receptor binding / regulation of triglyceride biosynthetic process / positive regulation of activation of membrane attack complex / vertebrate eye-specific patterning / positive regulation of apoptotic cell clearance / complement-mediated synapse pruning / Alternative complement activation / positive regulation of G protein-coupled receptor signaling pathway / positive regulation of lipid storage / positive regulation of phagocytosis, engulfment / complement receptor mediated signaling pathway / Activation of C3 and C5 / positive regulation of type IIa hypersensitivity / positive regulation of glucose transmembrane transport / complement-dependent cytotoxicity / complement activation, alternative pathway / complement activation / neuron remodeling / endopeptidase inhibitor activity / amyloid-beta clearance / positive regulation of vascular endothelial growth factor production / Purinergic signaling in leishmaniasis infection / Peptide ligand-binding receptors / fatty acid metabolic process / complement activation, classical pathway / Regulation of Complement cascade / Post-translational protein phosphorylation / response to bacterium / positive regulation of receptor-mediated endocytosis / positive regulation of angiogenesis / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / azurophil granule lumen / G alpha (i) signalling events / secretory granule lumen / blood microparticle / inflammatory response / positive regulation of protein phosphorylation / immune response / G protein-coupled receptor signaling pathway / endoplasmic reticulum lumen / signaling receptor binding / Neutrophil degranulation / cell surface / signal transduction / protein-containing complex / extracellular space / extracellular exosome / extracellular region / membrane / plasma membrane 類似検索 - 分子機能
ジャーナル: Elife / 年: 2024 タイトル: Molecular mechanism of complement inhibition by the trypanosome receptor ISG65. 著者: Alexander D Cook / Mark Carrington / Matthew K Higgins / 要旨: African trypanosomes replicate within infected mammals where they are exposed to the complement system. This system centres around complement C3, which is present in a soluble form in serum but ...African trypanosomes replicate within infected mammals where they are exposed to the complement system. This system centres around complement C3, which is present in a soluble form in serum but becomes covalently deposited onto the surfaces of pathogens after proteolytic cleavage to C3b. Membrane-associated C3b triggers different complement-mediated effectors which promote pathogen clearance. To counter complement-mediated clearance, African trypanosomes have a cell surface receptor, ISG65, which binds to C3b and which decreases the rate of trypanosome clearance in an infection model. However, the mechanism by which ISG65 reduces C3b function has not been determined. We reveal through cryogenic electron microscopy that ISG65 has two distinct binding sites for C3b, only one of which is available in C3 and C3d. We show that ISG65 does not block the formation of C3b or the function of the C3 convertase which catalyses the surface deposition of C3b. However, we show that ISG65 forms a specific conjugate with C3b, perhaps acting as a decoy. ISG65 also occludes the binding sites for complement receptors 2 and 3, which may disrupt recruitment of immune cells, including B cells, phagocytes, and granulocytes. This suggests that ISG65 protects trypanosomes by combining multiple approaches to dampen the complement cascade.
ダウンロード / ファイル: emd_17209.map.gz / 形式: CCP4 / 大きさ: 144.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Human Complement C3 in complex with T. brucei ISG65. Composite map from local alignments of the CUB-TED-ISG65 and remaining C3b regions. Postprocessed in DeepEMhancer.
ボクセルのサイズ
X=Y=Z: 0.832 Å
密度
表面レベル
登録者による: 0.15
最小 - 最大
-0.0018395517 - 1.873865
平均 (標準偏差)
0.0015222499 (±0.027328268)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
336
336
336
Spacing
336
336
336
セル
A=B=C: 279.552 Å α=β=γ: 90.0 °
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添付データ
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試料の構成要素
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全体 : Complex of Human Complement C3b and T. brucei ISG65
全体
名称: Complex of Human Complement C3b and T. brucei ISG65
要素
複合体: Complex of Human Complement C3b and T. brucei ISG65
複合体: Human Complement C3b
タンパク質・ペプチド: Complement C3f fragment
タンパク質・ペプチド: Complement C3
複合体: Trypanosoma brucei ISG65
タンパク質・ペプチド: ISG65 G
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超分子 #1: Complex of Human Complement C3b and T. brucei ISG65
超分子
名称: Complex of Human Complement C3b and T. brucei ISG65 / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all
分子量
理論値: 44.2 KDa
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超分子 #2: Human Complement C3b
超分子
名称: Human Complement C3b / タイプ: complex / ID: 2 / 親要素: 1 / 含まれる分子: #1-#2 詳細: C3 purified from human serum, C3b generated from C3 by limited trypsin proteolysis.