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- EMDB-16533: Cryo-EM structure of native Otu2-bound ubiquitinated 48S initiati... -
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Open data
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Basic information
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Title | Cryo-EM structure of native Otu2-bound ubiquitinated 48S initiation complex (partial) | ||||||||||||||||||
![]() | Cryo-EM map of Otu2-bound ubiquitinated 48S initiation complex | ||||||||||||||||||
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Function / homology | ![]() eukaryotic translation initiation factor 3 complex, eIF3e / eukaryotic translation initiation factor 3 complex, eIF3m / translation reinitiation / : / incipient cellular bud site / multi-eIF complex / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||||||||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() | ||||||||||||||||||
Method | ![]() ![]() | ||||||||||||||||||
![]() | Ikeuchi K / Buschauer R / Cheng J / Berninghausen O / Becker T / Beckmann R | ||||||||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Molecular basis for recognition and deubiquitination of 40S ribosomes by Otu2. Authors: Ken Ikeuchi / Nives Ivic / Robert Buschauer / Jingdong Cheng / Thomas Fröhlich / Yoshitaka Matsuo / Otto Berninghausen / Toshifumi Inada / Thomas Becker / Roland Beckmann / ![]() ![]() ![]() ![]() Abstract: In actively translating 80S ribosomes the ribosomal protein eS7 of the 40S subunit is monoubiquitinated by the E3 ligase Not4 and deubiquitinated by Otu2 upon ribosomal subunit recycling. Despite its ...In actively translating 80S ribosomes the ribosomal protein eS7 of the 40S subunit is monoubiquitinated by the E3 ligase Not4 and deubiquitinated by Otu2 upon ribosomal subunit recycling. Despite its importance for translation efficiency the exact role and structural basis for this translational reset is poorly understood. Here, structural analysis by cryo-electron microscopy of native and reconstituted Otu2-bound ribosomal complexes reveals that Otu2 engages 40S subunits mainly between ribosome recycling and initiation stages. Otu2 binds to several sites on the intersubunit surface of the 40S that are not occupied by any other 40S-binding factors. This binding mode explains the discrimination against 80S ribosomes via the largely helical N-terminal domain of Otu2 as well as the specificity for mono-ubiquitinated eS7 on 40S. Collectively, this study reveals mechanistic insights into the Otu2-driven deubiquitination steps for translational reset during ribosome recycling/(re)initiation. | ||||||||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 19.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 68.3 KB 68.3 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 13.9 KB | Display | ![]() |
Images | ![]() | 160.8 KB | ||
Others | ![]() ![]() | 261.9 MB 261.9 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8casMC ![]() 8c83C ![]() 8cahC ![]() 8cbjC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Cryo-EM map of Otu2-bound ubiquitinated 48S initiation complex | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.045 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half-map A
File | emd_16533_half_map_1.map | ||||||||||||
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Annotation | Half-map A | ||||||||||||
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Density Histograms |
-Half map: Half-map B
File | emd_16533_half_map_2.map | ||||||||||||
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Annotation | Half-map B | ||||||||||||
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Density Histograms |
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Sample components
+Entire : Yeast native 48S initiation complex (partial) with deubiquitinati...
+Supramolecule #1: Yeast native 48S initiation complex (partial) with deubiquitinati...
+Macromolecule #1: 40S ribosomal protein S0-A
+Macromolecule #2: 40S ribosomal protein S1-A
+Macromolecule #3: 40S ribosomal protein S2
+Macromolecule #4: 40S ribosomal protein S4-B
+Macromolecule #5: 40S ribosomal protein S6-B
+Macromolecule #6: 40S ribosomal protein S8-A
+Macromolecule #7: 40S ribosomal protein S9-A
+Macromolecule #8: 40S ribosomal protein S11-A
+Macromolecule #9: 40S ribosomal protein S13
+Macromolecule #10: 40S ribosomal protein S14-A
+Macromolecule #11: 40S ribosomal protein S21-A
+Macromolecule #12: 40S ribosomal protein S22-A
+Macromolecule #13: 40S ribosomal protein S23-A
+Macromolecule #14: 40S ribosomal protein S24-A
+Macromolecule #15: 40S ribosomal protein S26-A
+Macromolecule #16: 40S ribosomal protein S27-A
+Macromolecule #17: 40S ribosomal protein S30-A
+Macromolecule #18: RPS15 isoform 1
+Macromolecule #19: 40S ribosomal protein S3
+Macromolecule #20: 40S ribosomal protein S10-A
+Macromolecule #21: 40S ribosomal protein S12
+Macromolecule #22: 40S ribosomal protein S16-A
+Macromolecule #23: 40S ribosomal protein S17-A
+Macromolecule #24: 40S ribosomal protein S18-A
+Macromolecule #25: 40S ribosomal protein S19-A
+Macromolecule #26: 40S ribosomal protein S20
+Macromolecule #27: 40S ribosomal protein S25-A
+Macromolecule #28: RPS29A isoform 1
+Macromolecule #29: 40S ribosomal protein S31
+Macromolecule #30: Guanine nucleotide-binding protein subunit beta-like protein
+Macromolecule #31: 40S ribosomal protein S28-A
+Macromolecule #32: Eukaryotic translation initiation factor 3 subunit I
+Macromolecule #33: Eukaryotic translation initiation factor 3 subunit G
+Macromolecule #35: TIF5 isoform 1
+Macromolecule #36: Eukaryotic translation initiation factor 3 subunit A
+Macromolecule #37: Eukaryotic translation initiation factor 3 subunit B
+Macromolecule #38: Eukaryotic translation initiation factor 3 subunit C
+Macromolecule #40: Eukaryotic translation initiation factor 4C
+Macromolecule #41: RLI1 isoform 1
+Macromolecule #43: 40S ribosomal protein S5
+Macromolecule #44: 60S ribosomal protein L40-A
+Macromolecule #45: 60S ribosomal protein L41-A
+Macromolecule #46: SUI2 isoform 1
+Macromolecule #47: protein-synthesizing GTPase
+Macromolecule #48: SUI3 isoform 1
+Macromolecule #49: OTU domain-containing protein 2
+Macromolecule #50: 40S ribosomal protein S7-A
+Macromolecule #34: tRNA
+Macromolecule #39: mRNA
+Macromolecule #42: 18S ribosomal RNA
+Macromolecule #51: ZINC ION
+Macromolecule #52: ADENOSINE-5'-DIPHOSPHATE
+Macromolecule #53: MAGNESIUM ION
+Macromolecule #54: ADENOSINE-5'-TRIPHOSPHATE
+Macromolecule #55: IRON/SULFUR CLUSTER
+Macromolecule #56: METHIONINE
+Macromolecule #57: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 46.4 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |