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- EMDB-16312: In situ outer dynein arm from Chlamydomonas reinhardtii in the po... -

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Basic information

Entry
Database: EMDB / ID: EMD-16312
TitleIn situ outer dynein arm from Chlamydomonas reinhardtii in the post-power stroke state
Map data
Sample
  • Organelle or cellular component: In situ outer dynein arm
    • Protein or peptide: x 18 types
  • Ligand: x 3 types
Keywordsaxoneme / outer dynein arm / post power stroke / dynein / MOTOR PROTEIN
Function / homology
Function and homology information


outer dynein arm / outer dynein arm assembly / dynein light chain binding / cilium movement / dynein heavy chain binding / dynein complex / minus-end-directed microtubule motor activity / cytoplasmic dynein complex / dynein light intermediate chain binding / motile cilium ...outer dynein arm / outer dynein arm assembly / dynein light chain binding / cilium movement / dynein heavy chain binding / dynein complex / minus-end-directed microtubule motor activity / cytoplasmic dynein complex / dynein light intermediate chain binding / motile cilium / dynein intermediate chain binding / microtubule-based movement / microtubule-based process / microtubule / calcium ion binding / ATP hydrolysis activity / ATP binding / cytoplasm
Similarity search - Function
Kelch motif / Dynein light chain Tctex-1 like / Dynein heavy chain 3, AAA+ lid domain / Tctex-1-like superfamily / Tctex-1 family / Galactose oxidase, central domain / : / AAA+ lid domain / : / Dynein light chain, type 1/2 ...Kelch motif / Dynein light chain Tctex-1 like / Dynein heavy chain 3, AAA+ lid domain / Tctex-1-like superfamily / Tctex-1 family / Galactose oxidase, central domain / : / AAA+ lid domain / : / Dynein light chain, type 1/2 / Dynein light chain type 1 / Dynein light chain type 1 / Dynein light chain superfamily / Dynein heavy chain, AAA 5 extension domain / Dynein heavy chain AAA lid domain / Roadblock/LAMTOR2 domain / Roadblock/LC7 domain / Roadblock/LC7 domain / Dynein heavy chain, C-terminal domain / Dynein heavy chain, C-terminal domain, barrel region / Dynein heavy chain C-terminal domain / Filamin/ABP280 repeat / Filamin/ABP280 repeat profile. / Filamin/ABP280 repeat-like / Leucine rich repeat 4 / Leucine Rich repeats (2 copies) / P-loop containing dynein motor region / Dynein heavy chain, tail / Dynein heavy chain, N-terminal region 1 / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / EF hand / IPT/TIG domain / Kelch-type beta propeller / ig-like, plexins, transcription factors / Leucine-rich repeat, SDS22-like subfamily / Thioredoxin / IPT domain / : / Leucine-rich repeat profile. / Thioredoxin domain / Leucine-rich repeat / EF-hand domain pair / Leucine-rich repeat domain superfamily / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Immunoglobulin E-set / Thioredoxin-like superfamily / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Immunoglobulin-like fold / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Thioredoxin / Dynein light chain tctex-type 1 protein / Dynein intermediate chain 2 / Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protein, putative / Outer arm dynein beta heavy chain / Dynein light chain roadblock-type 2 protein / Dynein light chain / Dynein light chain / Dynein light chain / Dynein light chain ...Thioredoxin / Dynein light chain tctex-type 1 protein / Dynein intermediate chain 2 / Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protein, putative / Outer arm dynein beta heavy chain / Dynein light chain roadblock-type 2 protein / Dynein light chain / Dynein light chain / Dynein light chain / Dynein light chain / Dynein light chain 4A / Dynein light chain 2A / Dynein light chain 1 / Dynein heavy chain, outer arm protein / Dynein light chain roadblock-type 2 protein / Flagellar outer dynein arm intermediate protein, putative / Dynein light chain
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant) / Tetrahymena thermophila (eukaryote)
Methodsubtomogram averaging / cryo EM / Resolution: 30.3 Å
AuthorsZimmermann NEL / Noga A / Obbineni JM / Ishikawa T
Funding support Switzerland, 1 items
OrganizationGrant numberCountry
Swiss National Science FoundationNF310030_192644 Switzerland
CitationJournal: EMBO J / Year: 2023
Title: ATP-induced conformational change of axonemal outer dynein arms revealed by cryo-electron tomography.
Authors: Noemi Zimmermann / Akira Noga / Jagan Mohan Obbineni / Takashi Ishikawa /
Abstract: Axonemal outer dynein arm (ODA) motors generate force for ciliary beating. We analyzed three states of the ODA during the power stroke cycle using in situ cryo-electron tomography, subtomogram ...Axonemal outer dynein arm (ODA) motors generate force for ciliary beating. We analyzed three states of the ODA during the power stroke cycle using in situ cryo-electron tomography, subtomogram averaging, and classification. These states of force generation depict the prepower stroke, postpower stroke, and intermediate state conformations. Comparison of these conformations to published in vitro atomic structures of cytoplasmic dynein, ODA, and the Shulin-ODA complex revealed differences in the orientation and position of the dynein head. Our analysis shows that in the absence of ATP, all dynein linkers interact with the AAA3/AAA4 domains, indicating that interactions with the adjacent microtubule doublet B-tubule direct dynein orientation. For the prepower stroke conformation, there were changes in the tail that is anchored on the A-tubule. We built models starting with available high-resolution structures to generate a best-fitting model structure for the in situ pre- and postpower stroke ODA conformations, thereby showing that ODA in a complex with Shulin adopts a similar conformation as the active prepower stroke ODA in the axoneme.
History
DepositionDec 8, 2022-
Header (metadata) releaseMay 10, 2023-
Map releaseMay 10, 2023-
UpdateJun 28, 2023-
Current statusJun 28, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_16312.png

Downloads & links

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Map

FileDownload / File: emd_16312.map.gz / Format: CCP4 / Size: 1.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
8.49 Å/pix.
x 74 pix.
= 628.26 Å		8.49 Å/pix.
x 74 pix.
= 628.26 Å		8.49 Å/pix.
x 74 pix.
= 628.26 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 8.49 Å
Density

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Histogram (log scale)
Contour LevelBy AUTHOR: 0.14
Minimum - Maximum-0.797381 - 1.2221855
Average (Standard dev.)0.014285774 (±0.1958702)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions747474
Spacing747474
CellA=B=C: 628.26 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_16312_half_map_1.map
Projections & Slices
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Histogram

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Half map: #2

Fileemd_16312_half_map_2.map
Projections & Slices
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Sample components

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Entire : In situ outer dynein arm

EntireName: In situ outer dynein arm
Components
  • Organelle or cellular component: In situ outer dynein arm
    • Protein or peptide: Dynein heavy chain, outer arm protein
    • Protein or peptide: Outer arm dynein beta heavy chain
    • Protein or peptide: Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protein, putative
    • Protein or peptide: Dynein intermediate chain 2
    • Protein or peptide: Flagellar outer dynein arm intermediate protein, putative
    • Protein or peptide: Dynein light chain roadblock-type 2 protein
    • Protein or peptide: Dynein light chain roadblock-type 2 protein
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain tctex-type 1 protein
    • Protein or peptide: Dynein light chain 2A
    • Protein or peptide: Thioredoxin
    • Protein or peptide: Dynein light chain 1
    • Protein or peptide: Dynein light chain 4A
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION

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Supramolecule #1: In situ outer dynein arm

SupramoleculeName: In situ outer dynein arm / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1-#18
Source (natural)Organism: Chlamydomonas reinhardtii (plant)

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Macromolecule #1: Dynein heavy chain, outer arm protein

MacromoleculeName: Dynein heavy chain, outer arm protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 534.400938 KDa
SequenceString: MLSSKYSKRI AWMKTTICDS LQLKDMIVEE SFQYEKNKNL LEQFLSGEGL NKIFAYYQVQ EQAQNDDIKD TGAQDPVLFF TTGDLEKIQ DKAVWFLRIT NPADDKKKAS QQDGNDNDII FGEITPNTVP MLNALMESVY SRQIDHIITE KIQFWGVAEE E QVLEFQQH ...String:
MLSSKYSKRI AWMKTTICDS LQLKDMIVEE SFQYEKNKNL LEQFLSGEGL NKIFAYYQVQ EQAQNDDIKD TGAQDPVLFF TTGDLEKIQ DKAVWFLRIT NPADDKKKAS QQDGNDNDII FGEITPNTVP MLNALMESVY SRQIDHIITE KIQFWGVAEE E QVLEFQQH SNKFSSEVRE AINLMSPGTE HFKLDYEAIS GLSESEKMQH YEMKFNEWIN LISSQLNDDS EVRKDEKDAG PA TELIYWR SRMQKITNWS EQLKSKDFQI VKASLQRHKN HDNQRPRGDE SLSKLMMEYN RLDLLLTDKL NEAKDNVKYL TTL EKFIEP LYNGTPQQII DTLPALMNAI KMIHTIARFY NTTDKMTGLF IKITNQMIKN CKDRILNKKD NGDNPSLYKM IWEQ DPAEL IEVLGSCIKL YCEYKKCYND TKEKVADMPK GKTFDFSDAQ IFGKFDTFVR RLQKLIEIFS NIQQFNALAK HNLEG MDVL TNKFKKIIDD FKKKGHNLLD TANNKFDRDW VEFNVEISHL DGELQNFIDN NFNRFRNIEY SLKLLHKFQS TIKRDS LKH NLTSRYNAIL HNYATELDTI QRVFQDQKSN PPLVRNMPPE AGKIIWARHL FQKITGPINI FPENVINSTE IRRYYGS YN TLGKQLTIYE MWFYQDWVNK IEQSKAALQA TLIVRHDENK KLYVNFDLEI MQLIREAKCL DRQGIEIPES ARIILLQE D KFKTYYNELL YALKEYERIN SKIKPICKNL LLPHIEDLDL KLRPGMVTLT WTSMNIESYL YYVHQGLKKL EQLIINVND IIENRIENNL KTVSKVVLVH LPQDTKPLSL DSFVQLQEEY INSKTDFLTS KNVEVERAVD DLLQTIMLYP LDPHVDPVLP EETKRIKRY YFWYFYQALL NSTQNSLNAM KYRVCGKKIP GANTLQNLKP FFQVEVQLNG DKVTLNPSLQ EIQKSINRAA T AVLRCSKH LYNWDQQNKD STDKATFYDM IACDKEIVKV ILLLTGSIQG TKNKVNEFLS GFTKFEWLCK ESIQESIKKF SK NGPTLQN YEDQLKKFSQ IEEEIEKIVP TYKIGAMELM THNICTSLST WAKEWKLQYS QDLHKRARQL LDSLTEQTKM LST KLSKPV KDIDSLGYVM ETLEQIRKEQ AEIDMKFNPV QEMYSLLDNY LPGGITDKDE MDARSLLRRN WDILIQQAEI KGKE YQHKQ AIYLKELKQS IKDFTNQVSI FRRDYEKNGP MVEGISPAEA MERLRRFEDE YDVKYQMYKI NARGENLFGL QNQKY PELE KTDAEIKNLN KLYNLYDSVI KNIQQFKEKS WQDVSKDDLA KMEEDAGKYG EQCSRLPKDL KEWQAYRDLK NYIDSL REQ LPLIISLKKP SIMPRHWEKI KEITNTKLNY ENPDQFYIEE IMGAKLLDFR EDIEDITESA DKQLKIRTGL DEINLYW ND MQFQFGIWGK RDVPCMLNGL IVGTILERLE EDQLQLSTFN SQRHVTPFKA EVENLIRTFS DVNDTLDMWV KVQKLWTS L EPVFTGGDIA RQMPLQAKQF QGIDKNWMKI MEKAVETKKV IPCCQNDMLK DFLPDLNRKL EDCQKMLEAY LEGKRKKFP RFYFVSNPTL LKILSQGSEP TSIQEDFEKL FDAITKVTFE SAKDKKNPAL KQITQIQQVI GRNEENISLT GYYVKCEGNI EDWLKKLEQ NMQQTLKDIA SAAAQQVFQV GLKEFVSSQA SQIALLGLQI LWTSKVNEGL ERLSRNERNA MDIKRNEIKE H MNILSSMC LEDLNGAVER TKVETLVTIQ VHQKDISMDL KCKDVNDFEW QKQTRIAWKT DIDECIISIT DWDSPYSYEF LG AKERLCI TPLTDRCYIT LAQAMSMYYG GAPAGPAGTG KTETVKDLGR TLGVFVVVTN CSDQHRYRDM AKIFKGLVQS GLW GCFDEF NRIDLEVLSV VAMQVESITT ARKQHMKKFM FPEEEIEIEL IPTVSYFITM NPGYAGRQEL PENLKVLFRG VSMM VPDRE IIIKVKLASV GYLQIDLLAK KFNVLYRLCE EQLSKQRHYD FGLRNILSVL RTAGNTKRQE IKSDEEMLLM RSLRD MNLS KLVADDIPLF NGLLADIFPK LKEVPKKLYP DVEKKIPEEI NAESYLINTP SFQLKIIQLY ETCLVRHGFM LVGPTG SGK STIMKILTEV LTKLGSPHKI VIMNPKAITA EQMYGVKSEI SDDWIPGVFS TIWAKSNNRA LKHTTWITCD GPVDAIW IE NLNTVLDDNK ILTLANGERI AMTENCKVVF EVENLNNASP ATVSRCGQVY VSPTDLGYEA VIEGWIRNRK ASGRAEES D KLGNILRKYL INMRFIELQS KECKEPMMDT SPVISVINIL NLLTGCLQYF VQTQRTLSEQ EYEKFIVYSM AWAIGGIYE AQDRVRFHEL LLAKNAPIPQ KGKENETVFD YYVSQDYLDW KICSPEEWVP PQSLQFSQLL LPTLDSFRAE MLLNFILTQP KSHTCSNSA LLIGGSGTAK TSSVLLYCNK FDPQKMLFKR TNFSSATSPF MFQSTIEAEC DFKVGKEFAP PGNKMMTIFI D DMSMPFVN KWGDQITLEL VRQLIETGGF YMLDKTQRGN QRKMKNLQYI GAMNHPGGGR NDIPNRLKRQ FFIFNMILPL SI EGIYGPI IKHMFKQKYF SDSTYKVIES LTSATIALWN KVKSTMLPTP AKFHYVFNMR ELSRIFKGIL TCKKDTINDA PKS MKIKPE LFLVGLWRHE AERVLADKLV NNKDKDTVMG YIQEVSLESF SQIENEILEK YSSEKTFLFC DFLRPDVINE DGII EEEAP KIYEAIDSLT ELRKRCNFLL SFYNDRNPSK KMPLVLFDDA LKHLLRISRI IRQPRSSGLL VGVGGSGKQS LTRLA GFIG KNLIQQIIVT KTYSDKDLKE DIKKGFDDAG HLGKQVTFLM TDSEVKKEEF LEYINMVLST GEIPNLLAKD EREVWL GDI SQAYCKEKNL GNIDPPQSEL WTYFVDRVRD NFHIMLCFSP VGQKFRERAR KFPALFNECT IDWFLPWPEE ALVSVAE TF IKNFDKLDTK EETKQELMKH MGNVHLMVNE ICDEYYQKMR RQVYVTPKSF LSYLNSYKTL YIEKYDELDQ QEESFKIG L NKIQEATITI NQMEISLKEE EIQLNEATEK TNQLLANLDK ESKKANQKGE EVAATNKQCE IQAEQISKEK EEAERELEA ALPALRRAQE AVDSIESKDI VELKANKKPL DIIKYIMDAV LVFFKARLIP IQIEERVFNK KEGKAVLFLK ESYDESGIQT LGDMNFMKK LKEFEKDSIN EETIELLEPY LNQSEDWFND TFATKASKAA AGILKWAFAI YEYHQKSKIV KPKRIQVAIA E GRQAIALK ELEKAREDLA QIQAYIKNLK DVYTKQMEEK NELEMKAAKT KKKINTARTL ITSLSGEKDR WGKGAQDISD QK RKLVGNV SLSTAFISYC GPFNAEYRNK LAQQRFVVDM KKRGVPVTPG LELTSFLIDD ATIGEWNLQG LPKDDLSIQN GIM VTNSAR YPLFIDPQGQ GQNWIRNKLS ASIIPERCIT TLSHPKFKDM FLKYCMESGL TLIVENIENE VDPMMDPVLE RQII VKGKT QFVNVAGTEM ELSKEFKLFM TCRLANPSFS PELSAKTTII DFTVTQSGLE QQLLGKVISK EQKALEDSLN QLLAD VNQN QKDLQRLDKN LLERLINSQG NLLDDTELMD VLNNTKTQAK EVAAKLIDAE IKTKEINEKR EQYRPVAIRG SAIYFT MIE VSLVNWMYNS SLEQFLKLFI ESIDLSEKAQ LPSNRVKNII SFLTFHVYRY VNRGLFEKDK ITFILMMAFK ILTTAGT IS SGDVSLFLKS GDALDIKSER QKQISYLEDN QWLNILALSK HTFSGQTLPF FKELPDLISR SENQWRNWID KNDPENFP I PDFAESINQE KEIGSFISLC LVRSLRNDRT LIATQNFISN VLGKEFTDPI SYPIEGIWQE SSNMDPVLFL LSAGADPTS SIDELAKKKK KFPCEKVSMG EGQERVARQV IMKGFVEGGW VILQNCHLGL KFMEEIETLV SPINQIHEDF RLWITCEQHP KFPLGLLQK TLKVTNEPPK GLKAGLYKTF TTIITQEFID KVDHSNWRSL IFTICFLHSI VIERKKFGPL GWCVPYEYNY S DLEASLLY IEKYLTNLMS TPQPNSHNLP ISMNVVRYMI CEVQYGGRIT DDLDRELFIT YGETYLKDGI FGNDYFFYDI MV DGSGQKF KYRIPQNPSA ELIKYQEYIA KVPTVDNPEV FGLHSNADLT FRLKESKEMI NTVMETRPKD SSVGGGKTRE EIV QDKAKD MLKNLPPDYN DVEVRELVSK LGGPNPKTST ERGMTVPLNI FLYQEVTRMQ RVIGLVRKTL QDTILAIDGQ IIMT PEILE AINAIYDAKV PNSWLYDPSG AEISWLLPNL GSWSTSLSDR NKQLNDWLRS GQRPILFWLT GFFNPQGFLT GMKQE VTRN HKKKDGKGGE AWSLDDVVYS TTVKEREKEK DIEQPPAEGV YIKGLYLEGC KWSKNGLDDS DPKKIFADLP ILHVSA INK KKTNEQDRMS NTYLCPVYKY PKRTDKYLIF RVGLPCEGSN NPSHWKLRGV ALLCSTE

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Macromolecule #2: Outer arm dynein beta heavy chain

MacromoleculeName: Outer arm dynein beta heavy chain / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 530.182375 KDa
SequenceString: MGDHSQKDSP EDFIINRLSQ ALGIQKEKIK KSLETQQDDK GEVTNKDEFQ GFIQQDNSTN ILWVSGQSEK CTFYYGQLPP IDKFKKKGI AVIKLGLHKL TNENVAKDVV VVEITNNLLE HLNSVFNEIM SPVMQNPLNQ QGWTDLVAKD LMEKFNNYVA Q VYVLLGQI ...String:
MGDHSQKDSP EDFIINRLSQ ALGIQKEKIK KSLETQQDDK GEVTNKDEFQ GFIQQDNSTN ILWVSGQSEK CTFYYGQLPP IDKFKKKGI AVIKLGLHKL TNENVAKDVV VVEITNNLLE HLNSVFNEIM SPVMQNPLNQ QGWTDLVAKD LMEKFNNYVA Q VYVLLGQI KGKTMLPLPS HKLTSSDTTP DKDKAHVFEG SIITWTKQIK NVLKLEPEQL LKYGNDPGPL AEIEFWQNKR DN LNLIDSQ LKSVEVQNIL HFLDNNKSTY TTPFTKLQAE VKKARLEANE NYRYLFTLKD LFSKLQESQP SDFPTLYELF IPI MHTILL IYNKSKTYNQ PPRLVVLIRE ICNAIISNAQ AFVDKDTIFS LIDSKETTEA CDKLQVTLDV CSKFKDAYFE YKAK AGGNW KLTSNALFVR LDSFLERCQD ILHLTNTIVQ FNKLEKIELG GTKGKTLTES IAQIFKEFEE AVQAFTSVSY DIMNI AEKK FDDDFYEFRS KIKELERRLA SVITQGFDDY DTIYGRFKLL DNFEGLLTRP IIADELEKKH IVLLEMYKQD LKQVQS IFL EGKQFVDSMH ENAPLFLNMP PIAGALTWCK SLRDRIQEPI EKLAQLGQGI TEREEYKDVQ KLYTSITKSI KDYEDQK IL SWEKEVEDSS QDKLKQTLLC KDENDLIKVN FDPSLVRLLK EVKYFLLLRL EVPTTAKDIY TKAETYRTQI VALDMIVD N YNHIKTCLLP VEEPLVKKKI QDMEEEVKPG IEEIRWKSTN IDQFISKSKS IVDQLFETVN KMKDSLQKIH KSLANFNVK IIERKNRPMS PDDYDQFLKA IFSNKLTIVK DNGNQIQKLV KEVLDAVKAD KKQNSWKNYN DYVNVIVIEG ISTAIQTALL HLNEQINPV FIKRNDISPL FDIRLELGQS GIQFDPEIGE SSNQLTVRNT IRNWINDFFN IAGTIQRLDT TMPGDFLQEI R SFFEIKQC LAMITQNLEW IENECNQFRA RFDTYSYLWT EDEQISFNRF LDENEPKDED GKGGDDDEGE NTEKQNPLLK GC RAKIPNL DLFDEKITHL KAIQQEISRI KTPEDISWLR INLQPMKTAL DARVTRWIRV YTDFLVNQFR TTQKNLLDFI EKT KDGIKK NPADHENLHD KKLLMSVMKV ISDVKDVEPR REGIITRMKE MVTKLKKHNV PITEKGTDDP LQQIDNANSN FIEI YGRVF KVKADIIPLQ AEETQNIKRD LDIFMKEVES FRKEFMQKLP FDYTESMGYE NINNAYDTIM VYYHKLTAIE GRALE YNNL EKLFELQKSN YKQLKDCMND LKNLKTMWDA IALIHFQYND WKTKPWRQIK ADILLDTNKT LGTQIKNLPK EIRNFK GYN VIVEKVKNMG TVLPLVSALH SEFMEDRHWS QLKQITGTVF DHNSLSFYFE DILALNLYKY ENTVNEIVDV AQKEAKI EK KLKNIEQWWS KQVFEFTEYK ETKTFASLDN MMEVLDQHSL DLMGMKSQGK YVEFFYDRVE DWREKLGRVD VVVNEWLK V QKNWKILYNI FLLSEDIRMQ LPEDTKVFEG VDKEFKDMMS EVSANPSVVE ACTIERRDVL VGWSQAIKKC EKALNDYLE QKKKSFPRFY FLSNQSLLTI LSNGQNAPKV CEYLGDCFDG LKTLTFEPPA NPAETSKVGI GMISKDDEKV PFSSKFICEG AVEHWLLNL EFRMRETLQE ILEGAKNTAD LWDSGDKPRE EWVEGYNAQI ALLTTTIVWT EDVGRAFEDL AGGSETAMKE C QKLIEVRL ENLIKKVRGD LHILERWKII NIITIDVHSR DVVEKFVIQK VSEAESFAWL SQLKFYWENK PDSDMHLRQT LR FPWEKDK NKNKCIIRIV DWFRFYSYEY IGNAIRLVIT PLTDRCYITL TQALNLTMGG APAGPAGTGK TETTKDLGRA IGI PVMVFN CSDQMNKDSM AQIFMGLSQS GAWGCFDEFN RISIEVLSVV STQVKCVLDA LKEKKTKFSF VEEGEIQLQD TVGF FITMN PGYAGRTELP ENLKALFRSC AMVVPDLALI CENMLMSEGF TMARVLSRKF VSLYMLSREL LSKQKHYDWG LRAVK SVLR QAGKLKRGDP DMPEDPLLMR ALRDFNMPKI VTDDKVIFRR LIGDLFPKLD PPTKQNPELK KIVQDTTKKD MGLVAE ELF VTKVVQLAEI LEVRHCCFVI GPPGSGKTCV WKTLIKSYIN SGEDAEYDTL NPKAVTSDEL FGAYTKTKEW KNGVIAV IM KNQVKNEEKY KATHMHKWSV LDGDIDPEWI ESLNTVMDDN KVLTLVSNDR IFLTPQMRLI FEISNLRNAT PATVSRAG V LFINETDIGW MPYMNSWLER SQINILKQQK EMANMPEYPV IDDVAKSVFY RCFQSYFEQN IDVHDKNRVR HICPMVDIA MIQTICTILD ALLIQHLPKL KQMKEEDEKQ ALEAFFIFAG LWAIGGPVGG GQDDSKDMKE FNTVWKGAAK VKFPEQGLCY DYYYDINEN KWNTWKVEDY LPNDQPLFSK IYVATIHTTR LRYMIDIHLQ RRKPILFIGS AGTGKTAVVR DYLNSTRPEQ V SHKTINFS SFTDSLALQK NIESMVEKKN GRNYGSATNK VLICFIDDFN MPYVDKYGTQ SPIQLLRLIL DYGSIFNREQ LE ERKFLQD LLFFGCLNQK SGSFTVDLRL QRNFSVFSMY TPSSDVIKTI FGSILNAHLS TIDDKAQKMA FKLVEATYFT FDK ILKNTT AFAPSAKRFH YQFNFRELAR VCEGICRTTP GQYSGGDQGK LVRLWAHEMK RTFEDRFIAN EHVEFFRRYL TEAI SKCIG EFPETENPIA EPLIFTGFVA AHQGLDQQYT QCTIPVLKRV LDDKLEEYNE VKAQMNLVLF QQAMEHVSRI CRILD MPGN NALLVGVGGS GKQSLCRLST FINGFEIDQL VVTASFTIND LRNNLQEIYK KIAKPNSIAR VFMITDSQIK EEQFLI PIN DMLNSGWIFD LFPKEDMDSL VSGVRNEAKG EGVDVNNLTA LTSYFLDKIR KNLKVVLCFS PVGDTMRIRS RKFPGII NN TSIDWFHPWP HEALIDVAFR FLEEIEFPTE EIRQSISLNM AKVHSSIDTA NEKFLKLERR YNYTTPKSFL ELIDFYKK L LTEKRETIQR QIQRYEMGLN ILAETQNKVQ GLQEELKVKM VEVNKQREET DILIEKVGKE SALAEEEQTI ANAEEEKTN VAAAEAEKIS KEATEALAEA LPALRSAEAA VDCLKKPHVT EMKNLGSPPA GVIVTARVVL ILFNQGITLN DPDEKVWKKA VTFMNNPQA FIDKVKSFDG ENIEPNIIEQ SNKIIQDPSK KFNEKDMAGQ SYAASKLCAW AVNIVTFNKI FKQVKPLQDA Q KQANEILE EKKKELAIVK QRVAELNARV NSLKRQLEEA EARKMIVEQD AARCQSRLSA AENLVNGLAG ENKRWTQNVK FL KENIKSM IGDSLLASAF VSYIGAFSAK LRLELWKNTW LPDIIEKGIP ITEGIEPLKI LTTEAIKSKW KNEGLPADPM SLE NAAIIT ACARWPLIID PQLQGSTWIR GKQGENLTTI SLSQPKWLGA LTSSISSGRA VLIEGIQQEI DATLDPLLQR AVKK NGNQL QLEIGGDPID YDPNFKLFLM TKLINPHFRP EIAAQCTIIN FIVTESGLEE QFIAMVVNIE KNELEMAKQD LVKKQ NEYA VTLDKLESDL LQSLSEADPA TILDNTELIQ NLDKTKKTTI EITEQQQKAK VTEAEINIQR EHYRVVAAEG SMLYFL VIS LSVMDHMYQY SLESFITFFF KAINRTTVRD ENRIPTLILN IRQTIYQWIS RGLFEKHKLI FLTLIVFRLM QKKIIDV AY EVAEMDFLIK CPARPGVENT LDWLPNISWD QIQGLINLEE FRNFAHQLEK EAPNRFKDWY NELQPEDQKL PLDWKRLD S MPFKKLLVLR CLRPDRMTIS LNNFIRAVLP QGDAFVEMDQ KLAFSEILES VINEDSESTI PIFFILSPGS DPVKEVEKI AKKKRIEPGK NFFNIALGQG QDEIARRRIE EGNKEGHWVM LQNIHLMPTW LIELEKILDS YSGEAGGGNS EFRLFLSAEP STGIPIGIL DRSIKLTNEP PAGLKANMKR AWTYFSKEEI EDKDPKIKSI LFALCFFHST LIERRRFGPK GWNMSYPFNM G DLRDSYLV MNRYMEQNQG GKVPFNDLIY IFGEIMYGGH IVDDWDRRLC NSYLFNTMHE QLFDELELFP YIEGKGLSFK VP GQNPYEK YIEHIETSLK QETPLAYGLH PNAEIGFRTD QCKTLFNTLL ELMPKEQSRD EKSSDIKSSN EMASDLIKQL LED SELKNK IFNMEEIKNK IDAENKGPYQ NVFLQEIEYM NALLSEIVKD LEEIGQGLSG LLTVSENMEM IIESIALSRV PASW QKLAY PSKRGLQSWL ANLFQRIEQL NIFRDDPYSI PRVVMISRFF NPQSFLTAIM QVISRAKAYE LNKLYIQTEI TKRSI EEIE GAAKEGAYVY GFILEGARWD YQLGQLEESK PKEMFSVLPV TYCKAIPLPP EGKEDKSLYQ CPVYKTEDRG NTYVFT AQL KTRFPPRKWI LAGVAIIMDV EGVSDEVKKD KK

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Macromolecule #3: Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protei...

MacromoleculeName: Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protein, putative
type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 475.554406 KDa
SequenceString: MPQPLVWTQL KQTGTTQPTA RSGHTIITVG KTHIMFGGLD NDKNNYKDGK IAPNNQVFTL KLTQNNCEWR QIACQGDVPL PRCYHASCA ISADKMLVFG GSYTSNLRFN DTYILKTTSY QWSKPANQIS GGEPKNAESK IGAPQPRYGH SATFFEGKVY I FGGHGGIN ...String:
MPQPLVWTQL KQTGTTQPTA RSGHTIITVG KTHIMFGGLD NDKNNYKDGK IAPNNQVFTL KLTQNNCEWR QIACQGDVPL PRCYHASCA ISADKMLVFG GSYTSNLRFN DTYILKTTSY QWSKPANQIS GGEPKNAESK IGAPQPRYGH SATFFEGKVY I FGGHGGIN YQRLAFNDLY VLETENFEWT RLEPKGNPPD PRGGHSAAMM ANKPQLMIFG GWSFTSQYSN IMIYDIEKDE WV DPEIAHE IPKWNLSGIM APSIPSWKYF IFGGSVGSFE EGGNRTNSRF VDDSFVLDID TLSWSSINLE ADETSKAVCK PRP RESASI FYDSGESRAI VFGGWANNWL NDLWALNVST ITGPPYAIFS IKPALGPLTG KTKVLIEGDG FKDTQNISVK FSGG KLEKE VNGTFVNEKE ISCETPTFDY PRSVEVTVCM NKGDYTITKS AFTYYLNTKA DKTIAYGPGL LTENLIGVQT TIVIQ ARNK NDQNRESGSD EFVVTIRNPA KIKKEEEVKE GDKANTKNTI KEDEEEEGED EEENKKKKEA EKAAAEKAAA EKAAEE EQG EAVDPSLVPF NIVDNDDGSY FIQFTSEEEA VLEIDIKFKD ENNELHSIRG NPFRCGFVKG SKPNNNDLTG SAVMNYI SK QLKDIQEFIE NTKENIEIRN KNIRENVSEL INVMINLEKV RVKNDDDVLT LDTVEEMLNF LKKKDFGKDS DIKKCKKL Q EEWKNLAKMA QAVKKDIQNP VKTESDKTKE NIKKFEEITL KEYANSLKKE SFFIYKTGVS ESFKRIGEVK QKVDEFEVQ LNQYEDFARM FEFPDAVIGS KKLMEQIRTD VSSVEKLWVR IEISEKTMDE YKKMKWGSIN SMDMEDEIKK LRKALTDLRG IDKRSNAFI GITEELKKWA TFLPLLGELK DPSMNSEDGR HWKKLKDLVK KEFDVSQELM LEIIWDLKLF DYKDGIEEIT D QAKQELKM EKALKKIIDF WRDIEFELVQ HKNTDIHTLK MSEENFETLE DHQLQINNML LSKYVAYFEK EVEKWKYDLG SV YDVVQLL LEVQKTWSFL ENLFIQSEEV KRELPNESAQ FVGIDKDMKE IMQKGCDIKN CLKFCTIEGM LKRLENIQAQ LKV CEKALN EFLDSKRRAF PRFYFVSVND LLDILSNGNS PAKINRHMSK IFQAIDNLQL KEDSSGGRPT ALKMISCVGT EEVD FSSPR LLQGKVESYL KDVIDTMIGT LKSVANSSFK NFQSMTRKEW LKSDPSQITL LVNNIIWSKA VEDCFLKLQS GDINA MKLF LDESIKQLTE LIGMVQGDLS KPLRQKIMCL ITIDTHSRDV VHRLINEHVR KAEEFQWQSQ LKFYWVDNDA KIKIAD ARF VYNYEYLGNG PRLVITPLTD RIYVTATQAL HLKMGCAPAG PAGTGKTETT KDLANALAKA CYVFNCSSEM NYESMGN IY KGLASSGCWG CFDEFNRLLP EVLSVCSVQF KAVTDAIKQN VERFIIEGDE ISLDPTCGVF ITMNPGYLGR AELPEGLK A LFRPITVVVP DLELICENML MAEGFIEAKI LAKKFVTLYM LCRDLLSKQL HYDWGLRAIK SVLVVAGGFK RSEPEIAEQ ALLMRALRDF NIPKIAFQDL YVFHGLLGDL FPGINIKPKK DLDFEKIITD VCIENKLDPD PEFVLKVVQL SELLAIRHCV FVMGPPGAG KSTTWKILAK AQDKTNKKTT LIDIDPKVVS TKDFYGYNLP SKEWKDGLFS KMLRSLAEQP DTNPKWICLD G DLDANWIE SMNSVMDDNK ILTLANNERI PLKPHMRALF EIRDLRFATP ATVSRAGILY ISDEVGYQWR SYVKSWIKQE FS QDQEMSK NLDTLFGKYV PDTLDHIKKH CRFLVPVSPI SQVISICKSL QTLLKGDVKN LEYLFVYALI WAIGGALAEK DSI DYRKDF STWWKGAWKT AVKFPSKGTI FDYYVDQSGD SSKFVEWSKR LENKEFDPQV ETMGNITVNT IETLATTEFI KSYL MVKHP SLLIGNSGCG KTQLAKGILK EIVQAKPENY AYQLINFNYY TDSTYLQGQI EQTLEKKAGR QYGPPGKVQL IYFID DLNM PQLDAYDTQT AIALLRQLAD YGHFYDVSKL ALKDIINTQV LAAMNPSAGS FFVNPRYQRH FWTISIPFPD NESLSL IYI TFLNGHLKRF KSTIQEYSNI IVRASLMLHQ AVTQNFRKTA INFHYEFNLR HMSNVFQGLL LSDPNKFTEP DKLIKLW IH ECERTYGDRL VSTDNLKTYK ENIFDIVKKS FSKFNFSRYF GNNPENLIYC NFIAGINSDR FYDQMPNNEM EKHISEAL K EYNDNNAFMG LVLFEDAMKH VCRICRIVLP SSGHALLVGV GGSGKQSLSK LASFIMGYTT FSITISATYS MVDLRNDLQ QLYFKCGPKE EGILFLFTEG QITNERFLVY INDLLSSGEI AELYTLDEKE AMINQVRAKV KGEGKPDTRE NCWNWFIDQV KKNLHMAIC FSPVGDMRRR ARQFPALVNC TVIDWFQPWP YEALFNVAKS FLEPVDLGDD KVREAVVKFM PFSFTLVNDL G LKLLEQER RYAYTTPKSF LELISLFTNM LAQKRESLER NKERYETGLV KLKETAEQVA IIEVEVKEKQ VEAEAKKKEA DA FAEVVGR EKDKVEKENS KATIEADKCG LIKQNVEAQK SSTQQDLDAA QPLVEQAKSA LNSISKKDFQ QAKSFASPPA GVP EVFAAT IYLLAGYFNE AIEIDKNKKP KDVSWKSSLK LMKSPEEFME KLLNFKDVVD ANQVPAANVN IVKNQYLNMP SFTP EQMAS KSAAAKGICS WVVNIVKYYD VIQDVEPKRK ALKEATEQLE EATVKLNEVE EVVRKLNEEL NKLKAENDKA IAERN AAIS EAERCARRLN LAQRLVTALS SENERWGKSI IQLEDQLKLM VGDVLVASSF VSYSGPFNKK FRNIMINQNF MKFMKE HTI PMSPDPNPIK ILTDESTIAL WNKQKLPSDS VSIENGTILT NSARYPLMID PQLQGITWIR EKEKANNLKI LRLGSKN IN RDLELSIENG YSAIIENMNE RIDAILMPII ARSFIKRGKN KIIKFAGKDL ILHPNFKLFL HTKLSNPHYP PEIQAEAA L INFTVTEAGL GDQLLSLVVA RERPDLAKMK IELITQQNDF KIKLKDLEDE LLYKLANAKG DILDDIELIE NLEYSKKLS VEIAEKVAAA KITEAKINET SENYRPAASR GALFYFLLSD LSKVHSFYKY SLESFIVVIN RAIDAISENK IYGKTTMVPY GDETYASNQ HEKEEEEEEE EHQQAQQQQN QEEQQEQKDE ENKGPVEEEQ PEGENKGPVE EEGAEGQNEG PVEEENAENQ E GEGEEEGE KQQKAKDSDE PMSPRSLKKR VDELIESLTY TAYQTTRRGL FESHKLIVAA MLCLRVLLRS EELNSDEVDH LI IGKVDVN PTPMPDALKS FLNDNIWAAC KALETIHQFQ GFCQSLETDV LQWKKWYSEE KAETADLPKA FKELSKFHRL LLL RALRPD RLPSALSQFV HDKMGERYIE QPPFNIFETF QETSKTVPIF FVLFPGVDPT PDVERVAATF DVSANNGRFI NISM GQGQE DRAKKALFDC AQKGHWIMLQ NVHLMQSWLY GLNGLEGFLE SVFASPKTHP NFRVFISSEP PNVLLPLMQI IPESI LQGS LKIANEAPQY LKANLRRAYN KFDQEFLDKC DKKPTEFKSC LFALCFFHSL MLGRKKFGTQ GWSRVYNFND GDLTIC ADV LYNYLSKYDQ VPWDDLRYIF GEIMYGGHIT DDWDRRTNRT YLKVLIRSEL LQQNFNLAPQ FKSPDPSKFD YEAYRKY IE EKLPIESPQM FGMHPNAEIG YLTQTCDQVF NTILEVQGGS SGGGASKKDD GVMVTLTDFK TRCPHDFNML LIEEKVKE K TPYIVVCLQE CERMNGLLKE IKTSLEDLRL GLTGALNMTD AMESLQQSLS FNKVPDTWEK KAYFSKKPLS SWFADLIER NIQLQEWCKE LVTPTSLCIS YLFNPMSYLT AIMQFTARAQ GLPLDGITIQ TNVTAMKGPE DVVNPAENGA YIHGLFLEGA AWEIGGQGQ DGYLIEQKPK ELHPKMPVIN AVAVPLDKKK KNGQYDCPTY VTSARGQTFV FTANLNMESD DSDPNKWILS G TCMLMSDD

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Macromolecule #4: Dynein intermediate chain 2

MacromoleculeName: Dynein intermediate chain 2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 76.685891 KDa
SequenceString: MPPKQTKVVA SRKTVMPISR AGRAQIRRKD SNTQNNMNDQ GMEDEEIDQQ REGMKNQYEQ LTAQELNEDM PSKMLEPKNP QAPKNITVY DYYTRKFKTD ELVDQMIVHF SMDGDYIWKE SNEYKTQEEI RDTKKALIKE AMRKQESEEP GANHDEEAIK Q TLRNKFNY ...String:
MPPKQTKVVA SRKTVMPISR AGRAQIRRKD SNTQNNMNDQ GMEDEEIDQQ REGMKNQYEQ LTAQELNEDM PSKMLEPKNP QAPKNITVY DYYTRKFKTD ELVDQMIVHF SMDGDYIWKE SNEYKTQEEI RDTKKALIKE AMRKQESEEP GANHDEEAIK Q TLRNKFNY NTRECQTINP SIRERGVSTE PPPSDTICGN ITQWEIFDAY YAEIMKDHQI ENKKKKEVDQ DKKQDQSMYS TS FKRCCKI MERMVVQNDQ EDKYHDYRYY WSQGDNLEAG KNEGHLLPIW RFSNEKQRKK NVTSICWNPL YPDLFAVSLG SYD FTKQRM GLICLYSLKN TTHPEYAFNC EAGVMCLDFH PKSAALLAVG LYDGTVLVYD IRNKHKKPIY QSTVRNQKHT DPVW QVKWN PDTSKNYNFY SISSDGRVMN WILMKNKLEP EEVILLRLVG KNEEESTLIG LACGLCFDFN KFEPHIFLVG TEEGK IHKC SRAYSGQYQE TYNGHLLAVY KVKWNNFHPR TFISASADWT VRIWDSKYTS QIICFDLSMM VVDAVWAPYS STVFAC ATM DKVQVYDLNV DKLNKLAEQK IVKQPKLTNL SFNYKDPILL VGDSHGGVTL VKLSPNLCKS GPEIKQTEDK KAMEEFK NV KIEDYEREKM ENLLA

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Macromolecule #5: Flagellar outer dynein arm intermediate protein, putative

MacromoleculeName: Flagellar outer dynein arm intermediate protein, putative
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 77.178062 KDa
SequenceString: MAEYFTYSKK RKEFNNPINF QDTETRYGGI QNQVVNINQY VQRNPNFIDL DNIAELSEHS VNTERVKTGD RGMSHKEGGW PGNVDPNEA QETGRFKKRI EKDTSFPQAV KDLKEGVEKC IYQNNQIDLL EEYFEGETSE HVVENLSSKT LMLFKDEKEI C KRSVSEIS ...String:
MAEYFTYSKK RKEFNNPINF QDTETRYGGI QNQVVNINQY VQRNPNFIDL DNIAELSEHS VNTERVKTGD RGMSHKEGGW PGNVDPNEA QETGRFKKRI EKDTSFPQAV KDLKEGVEKC IYQNNQIDLL EEYFEGETSE HVVENLSSKT LMLFKDEKEI C KRSVSEIS WHPEGPTKVA VSYAIMRFQQ MPEKMPTQAY VWDLLNPNSP EIKLMSPSAV TNISYNQKIP DQIGGGCYNG LL AVWDGRK GENPIMISPV ENSHYEPVTH FHWLMSKTGS ECVTTSTDGK VMWWDTRKFE AGPVEKLNII EGLGENEEII GGT ALEYNV EAGPSKFLIG TESGSILTAN KKLKKPVEIT TRYGLDQGRH LGPVYSINRS NQNPKYFLSV GDWSCKIWVE DLKT PIIRT KYHGSYLSDG CWSPTRSGAF FLVRRDGWMD VWDYYYRQNE IAFSHKVSDS PLTCIKINQT GGAYHNSGKL CAIGD QDGT VTILELCDSL YTMQPKEKDI INEMFEREYR KEKNLETIKK QQELAKRQVQ KDMGSQKEKW EKKKLEMIET AEASFH ENL AKNPVNEEEF NELDSPSEKR KKTNQNQGRE QEEQSREEQE ASGNFNQQQQ QQQEEEQQQE GEQQHHQNQE HQNGQGH EN GQEEGEENGE EGNQQENEGQ EENEQQQE

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Macromolecule #6: Dynein light chain roadblock-type 2 protein

MacromoleculeName: Dynein light chain roadblock-type 2 protein / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 14.751817 KDa
SequenceString:
MAQDINADDQ LKQLSALEGA NGYVIFNESG IPLKRHEKSI SHEKAVHIAA LVSDLWNVSK KVIQRDLKTP ENDIEVIRLR TKHSYEYII TQSGDFTMLA IQLCGKAIEE AKKAAAAAAA AAVVQEENKE KEKK

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Macromolecule #7: Dynein light chain roadblock-type 2 protein

MacromoleculeName: Dynein light chain roadblock-type 2 protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 18.490188 KDa
SequenceString:
MIYKTSKEIQ KSDQRIIQQR VENSIINNQI YQKSLKRKTS KRKVRHQANK KNFQKEMSEV EDTLNRIKTH KTVLGYLIVN SEGGVVRGA FKDEEESKNI ANSIPLLTKK ARSVVRDLDP TNDLVFLRIQ TKLNEIMVAP DDEFSLIVIQ TKGEKRDEND

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Macromolecule #8: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 10.780357 KDa
SequenceString:
MSHLDKVQPV IKNSDMSVEM QKEVEEVAKK AIDYCNTDKE IATFIKDDFR SRYHGTWHCI VGRNFGSFVT FERSYYIYLY VGQLAILLF KTG

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Macromolecule #9: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 12.348086 KDa
SequenceString:
MEQEKAVTDM DINELRKLMI GKAIINSSDM QGDLLQEAQD VIQSGIENNS APVLNIEAAC KYIKENLDKK FGPTWQCIIG EGYAYDVTV QNNTLLFMFY NGNLAVLIFK S

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Macromolecule #10: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 11.435072 KDa
SequenceString:
QQYKTFMGAR VLWPPECADD ILEGAIRETQ DALKKFEIAR EGQKIAEHLK KYMDDHFDPY WHVFFGKNFG CQAVHNKNRF IYFYIEKTA FLMYQT

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Macromolecule #11: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 10.973408 KDa
SequenceString:
MGDHANEQII DMPENSEMKS MKNDAFSQAK FAVENYKFEN KISSHIKKFF DEKYGPNWHC VVGKHFNAYV SYDSKNFIFF YEGQLAILL YRKG

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Macromolecule #12: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 12.516457 KDa
SequenceString:
MSDSDSDEGR VVEEPLPPHI IRFNDMAQHL LKKVIRQADV LIKENPQGLE KDIALNLVKF VKSQPEFKIG DGEWQCIIGK NFGCSLTFD ANVLAFFDLL PSRKSILLFK SG

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Macromolecule #13: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 10.453167 KDa
SequenceString:
MNHEPEVKAT DMEEDMIKRV KEIAINAVKE YKQEKQIAHY IKYEFDKIDG YGWNCIVGRN FGSHIIHQTK KYIFFKINEL CLLLWKA

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Macromolecule #14: Dynein light chain tctex-type 1 protein

MacromoleculeName: Dynein light chain tctex-type 1 protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 13.202817 KDa
SequenceString:
MGDTDKEYIS EEVQKAIDDS VKQVFGIKDD SSQVTITYNK DKVNLWTQQI IDYTIRGLNK LGKHFKYCVT AILQQTNHAG ISVQITAYQ DTNTDGSLIQ CYEINDIYAI VSVFAMAV

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Macromolecule #15: Dynein light chain 2A

MacromoleculeName: Dynein light chain 2A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 15.60812 KDa
SequenceString:
MKGTYLYLNI YKRKREASLI TLNYIKNRFY PSKIQKIIKE LFEDRLKGVE YDPNNANQLS ERLVLELREK IKRGKVPRYK IGVQVVFGE IKGQGLRIAS KCLWDVQNDN YASYTYTSEK VYCTGIVFGC YFE

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Macromolecule #16: Thioredoxin

MacromoleculeName: Thioredoxin / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 14.256472 KDa
SequenceString:
MSKKQKKEIT TTVTSEDHFM TFYNENNKKL IVVDVYPGWS GPCTAMYPTY NQLMISIDDF EKRIDILLLD QDKLVTYKND KFHATCQPK FLFISEGKII DEVLGTNIPV FIEKVNKYIP LSY

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Macromolecule #17: Dynein light chain 1

MacromoleculeName: Dynein light chain 1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 22.851654 KDa
SequenceString: MSKGTTCQKA IVNWEAANPG KNPSEAEEIK LIFQIPPIEK MDGPVLNTLT KCKKLSLSSN SIDKMISLNM LRNLEILSLS RNVIKKISG LEDIGGTLRQ LWLSYNFIEK LDGLNNCSVL QTLYIGNNRI KNWEELDKLK DLPELENVLF YGNPIYEQVK E DPKLIVLK ...String:
MSKGTTCQKA IVNWEAANPG KNPSEAEEIK LIFQIPPIEK MDGPVLNTLT KCKKLSLSSN SIDKMISLNM LRNLEILSLS RNVIKKISG LEDIGGTLRQ LWLSYNFIEK LDGLNNCSVL QTLYIGNNRI KNWEELDKLK DLPELENVLF YGNPIYEQVK E DPKLIVLK KLPTLKNVDG YIIDDSVLEK VKQIADIPIS AKQL

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Macromolecule #18: Dynein light chain 4A

MacromoleculeName: Dynein light chain 4A / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 17.822795 KDa
SequenceString:
MASEVMNLSE EDIQFCQKAF SDYDEDGLGA IKVSDLKLAL ENIGCSLSEN DLFKMISEVD EKNTGLIKFS DFLNIYYKQK YANLGDDDQ DLVDAFVAMG GNADTTGSVD ANRLIQIVKE EFEMTIDIVG LIREIDKDGS GEIEYEEFKL LLKSGFANEG N

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Macromolecule #19: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 19 / Number of copies: 6 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

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Macromolecule #20: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 20 / Number of copies: 3 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #21: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 21 / Number of copies: 9 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 1.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 3.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 30.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 2131
ExtractionNumber tomograms: 8 / Number images used: 3167
Final angle assignmentType: MAXIMUM LIKELIHOOD

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-8bx8:
In situ outer dynein arm from Chlamydomonas reinhardtii in the post-power stroke state

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