登録情報 データベース : EMDB / ID : EMD-15757 ダウンロードとリンクタイトル Structure of RIP2K dimer bound to the XIAP BIR2 domain マップデータcryo EM map of RIP2 kinase dimer with bound the BIR2 domain of E3 ligase XIAP 詳細 試料複合体 : dimeric RIP2K bound to XIAP BIR2 domain複合体 : RIP2K dimerタンパク質・ペプチド : Receptor-interacting serine/threonine-protein kinase 2複合体 : BIR2 domain of E3 ligase XIAPタンパク質・ペプチド : E3 ubiquitin-protein ligase XIAPリガンド : ZINC ION 詳細 キーワード kinase / BIR2 / complex / dimer / IMMUNE SYSTEM機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
response to interleukin-18 / positive regulation of T-helper 1 cell differentiation / toll-like receptor 2 signaling pathway / endopeptidase regulator activity / regulation of apoptosis involved in tissue homeostasis / inhibition of cysteine-type endopeptidase activity / positive regulation of cytokine-mediated signaling pathway / positive regulation of protein linear polyubiquitination / immature T cell proliferation in thymus / regulation of BMP signaling pathway ... response to interleukin-18 / positive regulation of T-helper 1 cell differentiation / toll-like receptor 2 signaling pathway / endopeptidase regulator activity / regulation of apoptosis involved in tissue homeostasis / inhibition of cysteine-type endopeptidase activity / positive regulation of cytokine-mediated signaling pathway / positive regulation of protein linear polyubiquitination / immature T cell proliferation in thymus / regulation of BMP signaling pathway / positive regulation of T-helper 1 type immune response / copper ion homeostasis / positive regulation of xenophagy / LIM domain binding / xenophagy / nucleotide-binding oligomerization domain containing 1 signaling pathway / CD4-positive, alpha-beta T cell proliferation / positive regulation of protein K63-linked ubiquitination / regulation of nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway / positive regulation of stress-activated MAPK cascade / caspase binding / cellular response to muramyl dipeptide / CARD domain binding / positive regulation of immature T cell proliferation in thymus / JUN kinase kinase kinase activity / cellular response to peptidoglycan / response to interleukin-12 / positive regulation of CD4-positive, alpha-beta T cell proliferation / activation of cysteine-type endopeptidase activity / nucleotide-binding oligomerization domain containing 2 signaling pathway / SMAC, XIAP-regulated apoptotic response / Activation of caspases through apoptosome-mediated cleavage / Regulation of the apoptosome activity / SMAC (DIABLO) binds to IAPs / SMAC(DIABLO)-mediated dissociation of IAP:caspase complexes / positive regulation of macrophage cytokine production / TNFR1-induced proapoptotic signaling / RIPK1-mediated regulated necrosis / toll-like receptor 4 signaling pathway / cysteine-type endopeptidase inhibitor activity / regulation of innate immune response / response to exogenous dsRNA / cellular response to lipoteichoic acid / cysteine-type endopeptidase inhibitor activity involved in apoptotic process / protein K63-linked ubiquitination / negative regulation of tumor necrosis factor-mediated signaling pathway / positive regulation of type I interferon production / positive regulation of interferon-alpha production / signaling adaptor activity / canonical NF-kappaB signal transduction / protein serine/threonine kinase binding / positive regulation of chemokine production / stress-activated MAPK cascade / JNK cascade / lipopolysaccharide-mediated signaling pathway / p75NTR recruits signalling complexes / positive regulation of interleukin-12 production / ERK1 and ERK2 cascade / positive regulation of interleukin-2 production / positive regulation of interferon-beta production / Regulation of PTEN localization / response to interleukin-1 / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / positive regulation of peptidyl-threonine phosphorylation / positive regulation of interleukin-1 beta production / TNFR1-induced NF-kappa-B signaling pathway / positive regulation of protein ubiquitination / activated TAK1 mediates p38 MAPK activation / Deactivation of the beta-catenin transactivating complex / Regulation of TNFR1 signaling / positive regulation of JNK cascade / non-specific protein-tyrosine kinase / non-membrane spanning protein tyrosine kinase activity / TAK1-dependent IKK and NF-kappa-B activation / NOD1/2 Signaling Pathway / RING-type E3 ubiquitin transferase / protein homooligomerization / Regulation of necroptotic cell death / Wnt signaling pathway / cytokine-mediated signaling pathway / Regulation of PTEN stability and activity / Interleukin-1 signaling / ubiquitin-protein transferase activity / positive regulation of interleukin-6 production / positive regulation of peptidyl-tyrosine phosphorylation / positive regulation of tumor necrosis factor production / ubiquitin protein ligase activity / positive regulation of type II interferon production / Ovarian tumor domain proteases / positive regulation of canonical Wnt signaling pathway / Downstream TCR signaling / positive regulation of protein binding / positive regulation of peptidyl-serine phosphorylation / positive regulation of NF-kappaB transcription factor activity / T cell receptor signaling pathway / regulation of cell population proliferation / regulation of inflammatory response / regulation of apoptotic process / neuron apoptotic process 類似検索 - 分子機能 Receptor-interacting serine/threonine-protein kinase 2 / RIP2, CARD domain / XIAP/BIRC8, UBA domain / : / BIRC2/3-like, UBA domain / BIR repeat. / BIR repeat / Inhibitor of Apoptosis domain / BIR repeat profile. / Baculoviral inhibition of apoptosis protein repeat ... Receptor-interacting serine/threonine-protein kinase 2 / RIP2, CARD domain / XIAP/BIRC8, UBA domain / : / BIRC2/3-like, UBA domain / BIR repeat. / BIR repeat / Inhibitor of Apoptosis domain / BIR repeat profile. / Baculoviral inhibition of apoptosis protein repeat / CARD domain / CARD caspase recruitment domain profile. / Caspase recruitment domain / Zinc finger, C3HC4 type (RING finger) / Death-like domain superfamily / Ring finger / Zinc finger RING-type profile. / Zinc finger, RING-type / Protein tyrosine and serine/threonine kinase / Serine-threonine/tyrosine-protein kinase, catalytic domain / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Serine/Threonine protein kinases, catalytic domain / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily 類似検索 - ドメイン・相同性 Receptor-interacting serine/threonine-protein kinase 2 / E3 ubiquitin-protein ligase XIAP 類似検索 - 構成要素生物種 Homo sapiens (ヒト)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.15 Å 詳細 データ登録者Pellegrini E / Cusack S 資金援助 1件 詳細 詳細を隠すOrganization Grant number 国 Not funded
引用ジャーナル : Life Sci Alliance / 年 : 2023タイトル : Structure shows that the BIR2 domain of E3 ligase XIAP binds across the RIPK2 kinase dimer interface.著者 : Mathilde Lethier / Karine Huard / Michael Hons / Adrien Favier / Bernhard Brutscher / Elisabetta Boeri Erba / Derek W Abbott / Stephen Cusack / Erika Pellegrini / 要旨 : RIPK2 is an essential adaptor for NOD signalling and its kinase domain is a drug target for NOD-related diseases, such as inflammatory bowel disease. However, recent work indicates that the ... RIPK2 is an essential adaptor for NOD signalling and its kinase domain is a drug target for NOD-related diseases, such as inflammatory bowel disease. However, recent work indicates that the phosphorylation activity of RIPK2 is dispensable for signalling and that inhibitors of both RIPK2 activity and RIPK2 ubiquitination prevent the essential interaction between RIPK2 and the BIR2 domain of XIAP, the key RIPK2 ubiquitin E3 ligase. Moreover, XIAP BIR2 antagonists also block this interaction. To reveal the molecular mechanisms involved, we combined native mass spectrometry, NMR, and cryo-electron microscopy to determine the structure of the RIPK2 kinase BIR2 domain complex and validated the interface with in cellulo assays. The structure shows that BIR2 binds across the RIPK2 kinase antiparallel dimer and provides an explanation for both inhibitory mechanisms. It also highlights why phosphorylation of the kinase activation loop is dispensable for signalling while revealing the structural role of RIPK2-K209 residue in the RIPK2-XIAP BIR2 interaction. Our results clarify the features of the RIPK2 conformation essential for its role as a scaffold protein for ubiquitination. 履歴 登録 2022年9月5日 - ヘッダ(付随情報) 公開 2022年10月26日 - マップ公開 2022年10月26日 - 更新 2023年9月20日 - 現状 2023年9月20日 処理サイト : PDBe / 状態 : 公開
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