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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Trypanosoma brucei gambiense ISG65 in complex with human complement component C3 | |||||||||
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![]() | complement / complex / IMMUNE SYSTEM | |||||||||
機能・相同性 | ![]() oviduct epithelium development / C5L2 anaphylatoxin chemotactic receptor binding / regulation of triglyceride biosynthetic process / positive regulation of activation of membrane attack complex / vertebrate eye-specific patterning / positive regulation of apoptotic cell clearance / complement-mediated synapse pruning / Alternative complement activation / positive regulation of lipid storage / positive regulation of phagocytosis, engulfment ...oviduct epithelium development / C5L2 anaphylatoxin chemotactic receptor binding / regulation of triglyceride biosynthetic process / positive regulation of activation of membrane attack complex / vertebrate eye-specific patterning / positive regulation of apoptotic cell clearance / complement-mediated synapse pruning / Alternative complement activation / positive regulation of lipid storage / positive regulation of phagocytosis, engulfment / positive regulation of G protein-coupled receptor signaling pathway / complement receptor mediated signaling pathway / Activation of C3 and C5 / positive regulation of type IIa hypersensitivity / positive regulation of glucose transmembrane transport / complement-dependent cytotoxicity / complement activation, alternative pathway / complement activation / neuron remodeling / endopeptidase inhibitor activity / amyloid-beta clearance / positive regulation of vascular endothelial growth factor production / Purinergic signaling in leishmaniasis infection / Peptide ligand-binding receptors / complement activation, classical pathway / fatty acid metabolic process / Regulation of Complement cascade / Post-translational protein phosphorylation / response to bacterium / positive regulation of receptor-mediated endocytosis / positive regulation of angiogenesis / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / azurophil granule lumen / G alpha (i) signalling events / secretory granule lumen / blood microparticle / inflammatory response / immune response / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / endoplasmic reticulum lumen / signaling receptor binding / Neutrophil degranulation / cell surface / signal transduction / protein-containing complex / extracellular space / extracellular exosome / extracellular region / membrane / plasma membrane 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.58 Å | |||||||||
![]() | Suelzen H / Zoll S | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Cryo-EM structures of Trypanosoma brucei gambiense ISG65 with human complement C3 and C3b and their roles in alternative pathway restriction. 著者: Hagen Sülzen / Jakub Began / Arun Dhillon / Sami Kereïche / Petr Pompach / Jitka Votrubova / Farnaz Zahedifard / Adriana Šubrtova / Marie Šafner / Martin Hubalek / Maaike Thompson / ...著者: Hagen Sülzen / Jakub Began / Arun Dhillon / Sami Kereïche / Petr Pompach / Jitka Votrubova / Farnaz Zahedifard / Adriana Šubrtova / Marie Šafner / Martin Hubalek / Maaike Thompson / Martin Zoltner / Sebastian Zoll / ![]() ![]() ![]() 要旨: African Trypanosomes have developed elaborate mechanisms to escape the adaptive immune response, but little is known about complement evasion particularly at the early stage of infection. Here we ...African Trypanosomes have developed elaborate mechanisms to escape the adaptive immune response, but little is known about complement evasion particularly at the early stage of infection. Here we show that ISG65 of the human-infective parasite Trypanosoma brucei gambiense is a receptor for human complement factor C3 and its activation fragments and that it takes over a role in selective inhibition of the alternative pathway C5 convertase and thus abrogation of the terminal pathway. No deposition of C4b, as part of the classical and lectin pathway convertases, was detected on trypanosomes. We present the cryo-electron microscopy (EM) structures of native C3 and C3b in complex with ISG65 which reveal a set of modes of complement interaction. Based on these findings, we propose a model for receptor-ligand interactions as they occur at the plasma membrane of blood-stage trypanosomes and may facilitate innate immune escape of the parasite. | |||||||||
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-EMDBアーカイブ
マップデータ | ![]() | 122 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 19.7 KB 19.7 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 13.2 KB | 表示 | ![]() |
画像 | ![]() | 73.5 KB | ||
Filedesc metadata | ![]() | 7.2 KB | ||
その他 | ![]() ![]() | 226.5 MB 226.5 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7zgjMC ![]() 7zgkC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 0.86 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: #2
ファイル | emd_14707_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_14707_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
-全体 : Trypanosoma brucei gambiense ISG65 in complex with human compleme...
全体 | 名称: Trypanosoma brucei gambiense ISG65 in complex with human complement component C3 |
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要素 |
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-超分子 #1: Trypanosoma brucei gambiense ISG65 in complex with human compleme...
超分子 | 名称: Trypanosoma brucei gambiense ISG65 in complex with human complement component C3 タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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-超分子 #2: Complement C3 beta chain
超分子 | 名称: Complement C3 beta chain / タイプ: complex / ID: 2 / 親要素: 1 / 含まれる分子: #1 |
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由来(天然) | 生物種: ![]() |
-超分子 #3: 65 kDa invariant surface glycoprotein
超分子 | 名称: 65 kDa invariant surface glycoprotein / タイプ: complex / ID: 3 / 親要素: 1 / 含まれる分子: #3 |
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由来(天然) | 生物種: ![]() ![]() |
-超分子 #4: Complement C3 alpha chain
超分子 | 名称: Complement C3 alpha chain / タイプ: complex / ID: 4 / 親要素: 1 / 含まれる分子: #2 |
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由来(天然) | 生物種: ![]() |
-分子 #1: Complement C3 beta chain
分子 | 名称: Complement C3 beta chain / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 71.39332 KDa |
配列 | 文字列: SPMYSIITPN ILRLESEETM VLEAHDAQGD VPVTVTVHDF PGKKLVLSSE KTVLTPATNH MGNVTFTIPA NREFKSEKGR NKFVTVQAT FGTQVVEKVV LVSLQSGYLF IQTDKTIYTP GSTVLYRIFT VNHKLLPVGR TVMVNIENPE GIPVKQDSLS S QNQLGVLP ...文字列: SPMYSIITPN ILRLESEETM VLEAHDAQGD VPVTVTVHDF PGKKLVLSSE KTVLTPATNH MGNVTFTIPA NREFKSEKGR NKFVTVQAT FGTQVVEKVV LVSLQSGYLF IQTDKTIYTP GSTVLYRIFT VNHKLLPVGR TVMVNIENPE GIPVKQDSLS S QNQLGVLP LSWDIPELVN MGQWKIRAYY ENSPQQVFST EFEVKEYVLP SFEVIVEPTE KFYYIYNEKG LEVTITARFL YG KKVEGTA FVIFGIQDGE QRISLPESLK RIPIEDGSGE VVLSRKVLLD GVQNPRAEDL VGKSLYVSAT VILHSGSDMV QAE RSGIPI VTSPYQIHFT KTPKYFKPGM PFDLMVFVTN PDGSPAYRVP VAVQGEDTVQ SLTQGDGVAK LSINTHPSQK PLSI TVRTK KQELSEAEQA TRTMQALPYS TVGNSNNYLH LSVLRTELRP GETLNVNFLL RMDRAHEAKI RYYTYLIMNK GRLLK AGRQ VREPGQDLVV LPLSITTDFI PSFRLVAYYT LIGASGQREV VADSVWVDVK DSCVGSLVVK SGQSEDRQPV PGQQMT LKI EGDHGARVVL VAVDKGVFVL NKKNKLTQSK IWDVVEKADI GCTPGSGKDY AGVFSDAGLT FTSSSGQQTA QRAELQC PQ PAA UniProtKB: Complement C3 |
-分子 #2: Complement C3
分子 | 名称: Complement C3 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 113.169875 KDa |
配列 | 文字列: SVQLTEKRMD KVGKYPKELR KCCEDGMREN PMRFSCQRRT RFISLGEACK KVFLDCCNYI TELRRQHARA SHLGLARSNL DEDIIAEEN IVSRSEFPES WLWNVEDLKE PPKNGISTKL MNIFLKDSIT TWEILAVSMS DKKGICVADP FEVTVMQDFF I DLRLPYSV ...文字列: SVQLTEKRMD KVGKYPKELR KCCEDGMREN PMRFSCQRRT RFISLGEACK KVFLDCCNYI TELRRQHARA SHLGLARSNL DEDIIAEEN IVSRSEFPES WLWNVEDLKE PPKNGISTKL MNIFLKDSIT TWEILAVSMS DKKGICVADP FEVTVMQDFF I DLRLPYSV VRNEQVEIRA VLYNYRQNQE LKVRVELLHN PAFCSLATTK RRHQQTVTIP PKSSLSVPYV IVPLKTGLQE VE VKAAVYH HFISDGVRKS LKVVPEGIRM NKTVAVRTLD PERLGREGVQ KEDIPPADLS DQVPDTESET RILLQGTPVA QMT EDAVDA ERLKHLIVTP SGCGEQNMIG MTPTVIAVHY LDETEQWEKF GLEKRQGALE LIKKGYTQQL AFRQPSSAFA AFVK RAPST WLTAYVVKVF SLAVNLIAID SQVLCGAVKW LILEKQKPDG VFQEDAPVIH QEMIGGLRNN NEKDMALTAF VLISL QEAK DICEEQVNSL PGSITKAGDF LEANYMNLQR SYTVAIAGYA LAQMGRLKGP LLNKFLTTAK DKNRWEDPGK QLYNVE ATS YALLALLQLK DFDFVPPVVR WLNEQRYYGG GYGSTQATFM VFQALAQYQK DAPDHQELNL DVSLQLPSRS SKITHRI HW ESASLLRSEE TKENEGFTVT AEGKGQGTLS VVTMYHAKAK DQLTCNKFDL KVTIKPAPET EKRPQDAKNT MILEICTR Y RGDQDATMSI LDISMMTGFA PDTDDLKQLA NGVDRYISKY ELDKAFSDRN TLIIYLDKVS HSEDDCLAFK VHQYFNVEL IQPGAVKVYA YYNLEESCTR FYHPEKEDGK LNKLCRDELC RCAEENCFIQ KSDDKVTLEE RLDKACEPGV DYVYKTRLVK VQLSNDFDE YIMAIEQTIK SGSDEVQVGQ QRTFISPIKC REALKLEEKK HYLMWGLSSD FWGEKPNLSY IIGKDTWVEH W PEEDECQD EENQKQCQDL GAFTESMVVF GCPN UniProtKB: Complement C3 |
-分子 #3: 65 kDa invariant surface glycoprotein, putative
分子 | 名称: 65 kDa invariant surface glycoprotein, putative / タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() 株: MHOM/CI/86/DAL972 |
分子量 | 理論値: 40.823516 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: MGSSHHHHHH SSGLVPRGSH MLLVIGSEDN RVPGDKKLTK EGAAALCKMK HLADKVAKER SQELKDRTQN FAGYIEFELY RIDYWLEKL NGPKGRKDGY AKLSDSDIEK VKEIFNKAKD GITKQLPEAK KAGEEAGKLH TEVKKAAENA RGQDLDDDTA K STGLYRVL ...文字列: MGSSHHHHHH SSGLVPRGSH MLLVIGSEDN RVPGDKKLTK EGAAALCKMK HLADKVAKER SQELKDRTQN FAGYIEFELY RIDYWLEKL NGPKGRKDGY AKLSDSDIEK VKEIFNKAKD GITKQLPEAK KAGEEAGKLH TEVKKAAENA RGQDLDDDTA K STGLYRVL NWYCITKEER HNATPNCDGI QFRKHYLSVN RSAIDCSSTS YEENYDWSAN ALQVALNSWE DVKPKKLESA GS DKNCNIG QSSESHPCTM TEEWQTPYKE TVEKLRELED AYQRGKKAHD AMLGYANTAY AVNTKVEQEK PLTEVIAAAK EAG KKGAKI IIPAAAPATP TNSTKNDDSA PTEHVDRGIA TNETQVEVGI D UniProtKB: 65 kDa invariant surface glycoprotein, putative |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 構成要素:
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グリッド | モデル: C-flat-1.2/1.3 / 材質: COPPER / メッシュ: 300 / 前処理 - タイプ: GLOW DISCHARGE | |||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 41.1 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 1.5 µm |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |