+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-14180 | |||||||||
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タイトル | Human Oxytocin receptor (OTR) oxytocin Gq chimera (mGoqi) complex | |||||||||
マップデータ | primary map | |||||||||
試料 |
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機能・相同性 | 機能・相同性情報 oxytocin receptor activity / positive regulation of hindgut contraction / response to anoxia / maternal aggressive behavior / oxytocin receptor binding / neurohypophyseal hormone activity / V1A vasopressin receptor binding / guanyl nucleotide binding / Vasopressin-like receptors / regulation of systemic arterial blood pressure by vasopressin ...oxytocin receptor activity / positive regulation of hindgut contraction / response to anoxia / maternal aggressive behavior / oxytocin receptor binding / neurohypophyseal hormone activity / V1A vasopressin receptor binding / guanyl nucleotide binding / Vasopressin-like receptors / regulation of systemic arterial blood pressure by vasopressin / vasopressin receptor activity / positive regulation of penile erection / maternal process involved in parturition / response to prostaglandin E / positive regulation of uterine smooth muscle contraction / positive regulation of norepinephrine secretion / negative regulation of urine volume / sperm ejaculation / response to sucrose / telencephalon development / drinking behavior / positive regulation of prostaglandin secretion / grooming behavior / response to ether / mu-type opioid receptor binding / corticotropin-releasing hormone receptor 1 binding / positive regulation of blood pressure / maternal behavior / neuropeptide hormone activity / positive regulation of ossification / digestive tract development / vesicle docking involved in exocytosis / positive regulation of synapse assembly / positive regulation of female receptivity / response to food / G protein-coupled dopamine receptor signaling pathway / regulation of heart contraction / eating behavior / male mating behavior / suckling behavior / peptide hormone binding / microvillus / positive regulation of vasoconstriction / social behavior / estrous cycle / negative regulation of insulin secretion / positive regulation of synaptic transmission / G protein-coupled serotonin receptor binding / positive regulation of synaptic transmission, glutamatergic / muscle contraction / response to electrical stimulus / response to retinoic acid / response to glucocorticoid / cellular response to hormone stimulus / response to cAMP / response to amphetamine / ERK1 and ERK2 cascade / negative regulation of blood pressure / lactation / regulation of heart rate / peptide binding / secretory granule / response to cocaine / response to activity / response to cytokine / locomotory behavior / response to progesterone / positive regulation of synaptic transmission, GABAergic / female pregnancy / adherens junction / G-protein beta/gamma-subunit complex binding / Olfactory Signaling Pathway / Activation of the phototransduction cascade / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / terminal bouton / G protein-coupled acetylcholine receptor signaling pathway / G-protein activation / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Prostacyclin signalling through prostacyclin receptor / Glucagon signaling in metabolic regulation / G beta:gamma signalling through CDC42 / memory / response to peptide hormone / ADP signalling through P2Y purinoceptor 12 / G beta:gamma signalling through BTK / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / Sensory perception of sweet, bitter, and umami (glutamate) taste / photoreceptor disc membrane / Adrenaline,noradrenaline inhibits insulin secretion / Glucagon-type ligand receptors / Vasopressin regulates renal water homeostasis via Aquaporins / G alpha (z) signalling events / cellular response to catecholamine stimulus / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADORA2B mediated anti-inflammatory cytokines production / sensory perception of taste 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) / Mus musculus (ハツカネズミ) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.25 Å | |||||||||
データ登録者 | Waltenspuhl Y / Ehrenmann J / Vacca S / Thom C / Medalia O / Pluckthun A | |||||||||
資金援助 | スイス, 1件
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引用 | ジャーナル: Nat Commun / 年: 2022 タイトル: Structural basis for the activation and ligand recognition of the human oxytocin receptor. 著者: Yann Waltenspühl / Janosch Ehrenmann / Santiago Vacca / Cristian Thom / Ohad Medalia / Andreas Plückthun / 要旨: The small cyclic neuropeptide hormone oxytocin (OT) and its cognate receptor play a central role in the regulation of social behaviour and sexual reproduction. Here we report the single-particle cryo- ...The small cyclic neuropeptide hormone oxytocin (OT) and its cognate receptor play a central role in the regulation of social behaviour and sexual reproduction. Here we report the single-particle cryo-electron microscopy structure of the active oxytocin receptor (OTR) in complex with its cognate ligand oxytocin. Our structure provides high-resolution insights into the OT binding mode, the OTR activation mechanism as well as the subtype specificity within the oxytocin/vasopressin receptor family. | |||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_14180.map.gz | 135.6 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-14180-v30.xml emd-14180.xml | 20.1 KB 20.1 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_14180.png | 52.9 KB | ||
その他 | emd_14180_additional_1.map.gz | 129.4 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-14180 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-14180 | HTTPS FTP |
-関連構造データ
関連構造データ | 7qvmMC M: このマップから作成された原子モデル C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_14180.map.gz / 形式: CCP4 / 大きさ: 266.8 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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注釈 | primary map | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.651 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-追加マップ: raw map
ファイル | emd_14180_additional_1.map | ||||||||||||
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注釈 | raw map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : OTR in complex with Oxytocin, heterotrimeric mini-Gq chimera (Goq...
+超分子 #1: OTR in complex with Oxytocin, heterotrimeric mini-Gq chimera (Goq...
+超分子 #2: Heterotrimeric mini-Gq chimera (Goqi) and Oxytocin receptor
+超分子 #3: Oxytocin
+超分子 #4: Antibody fragment scFv16
+分子 #1: Guanine nucleotide-binding protein G(o) subunit alpha,cDNA FLJ314...
+分子 #2: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
+分子 #3: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
+分子 #4: Oxytocin
+分子 #5: Oxytocin receptor
+分子 #6: Antibody fragment scFv16
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 63.78 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: OTHER |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 0.8 µm |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
CTF補正 | ソフトウェア - 名称: Gctf |
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最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.25 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 392369 |
初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |