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- EMDB-13703: Emodepside-bound Drosophila Slo channel -

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Basic information

Entry
Database: EMDB / ID: EMD-13703
TitleEmodepside-bound Drosophila Slo channel
Map dataSharpened by Phenix.Autosharpen
Sample
  • Complex: Slo tetramer
    • Protein or peptide: Isoform J of Calcium-activated potassium channel slowpoke
  • Ligand: CALCIUM ION
  • Ligand: MAGNESIUM ION
  • Ligand: (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE
  • Ligand: CHOLESTEROL
  • Ligand: POTASSIUM ION
  • Ligand: (3~{S},6~{R},9~{S},12~{R},15~{S},18~{R},21~{S},24~{R})-4,6,10,16,18,22-hexamethyl-3,9,15,21-tetrakis(2-methylpropyl)-12,24-bis[(4-morpholin-4-ylphenyl)methyl]-1,7,13,19-tetraoxa-4,10,16,22-tetrazacyclotetracosane-2,5,8,11,14,17,20,23-octone
Function / homology
Function and homology information


Sperm Motility And Taxes / negative regulation of neuromuscular synaptic transmission / male courtship behavior, veined wing generated song production / regulation of synaptic assembly at neuromuscular junction / large conductance calcium-activated potassium channel activity / calcium-activated potassium channel activity / circadian behavior / monoatomic ion channel complex / potassium ion transmembrane transport / potassium ion transport ...Sperm Motility And Taxes / negative regulation of neuromuscular synaptic transmission / male courtship behavior, veined wing generated song production / regulation of synaptic assembly at neuromuscular junction / large conductance calcium-activated potassium channel activity / calcium-activated potassium channel activity / circadian behavior / monoatomic ion channel complex / potassium ion transmembrane transport / potassium ion transport / circadian rhythm / postsynaptic membrane / neuron projection / response to xenobiotic stimulus / neuronal cell body / membrane / plasma membrane
Similarity search - Function
: / Ca2+-activated K+ channel Slowpoke, TrkA_C like domain / Calcium-activated potassium channel BK, alpha subunit / : / Calcium-activated BK potassium channel alpha subunit / Calcium-activated potassium channel slowpoke-like RCK domain / RCK N-terminal domain profile. / Ion transport domain / Ion transport protein / NAD(P)-binding domain superfamily
Similarity search - Domain/homology
Calcium-activated potassium channel slowpoke
Similarity search - Component
Biological speciesDrosophila melanogaster (fruit fly)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.59 Å
AuthorsRaisch T / Brockmann A / Ebbinghaus-Kintscher U / Freigang J / Gutbrod O / Kubicek J / Maertens B / Hofnagel O / Raunser S
Funding support Germany, 1 items
OrganizationGrant numberCountry
Max Planck Society Germany
CitationJournal: Nat Commun / Year: 2021
Title: Small molecule modulation of the Drosophila Slo channel elucidated by cryo-EM.
Authors: Tobias Raisch / Andreas Brockmann / Ulrich Ebbinghaus-Kintscher / Jörg Freigang / Oliver Gutbrod / Jan Kubicek / Barbara Maertens / Oliver Hofnagel / Stefan Raunser /
Abstract: Slowpoke (Slo) potassium channels display extraordinarily high conductance, are synergistically activated by a positive transmembrane potential and high intracellular Ca concentrations and are ...Slowpoke (Slo) potassium channels display extraordinarily high conductance, are synergistically activated by a positive transmembrane potential and high intracellular Ca concentrations and are important targets for insecticides and antiparasitic drugs. However, it is unknown how these compounds modulate ion translocation and whether there are insect-specific binding pockets. Here, we report structures of Drosophila Slo in the Ca-bound and Ca-free form and in complex with the fungal neurotoxin verruculogen and the anthelmintic drug emodepside. Whereas the architecture and gating mechanism of Slo channels are conserved, potential insect-specific binding pockets exist. Verruculogen inhibits K transport by blocking the Ca-induced activation signal and precludes K from entering the selectivity filter. Emodepside decreases the conductance by suboptimal K coordination and uncouples ion gating from Ca and voltage sensing. Our results expand the mechanistic understanding of Slo regulation and lay the foundation for the rational design of regulators of Slo and other voltage-gated ion channels.
History
DepositionOct 8, 2021-
Header (metadata) releaseDec 15, 2021-
Map releaseDec 15, 2021-
UpdateDec 22, 2021-
Current statusDec 22, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 5
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 5
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7pxh
  • Surface level: 5
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_13703.png

Downloads & links

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Map

FileDownload / File: emd_13703.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationSharpened by Phenix.Autosharpen
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
0.7 Å/pix.
x 448 pix.
= 313.6 Å		0.7 Å/pix.
x 448 pix.
= 313.6 Å		0.7 Å/pix.
x 448 pix.
= 313.6 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.7 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 5.0 / Movie #1: 5
Minimum - Maximum-19.145393 - 36.510284
Average (Standard dev.)-1.8869494e-12 (±1.0)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions448448448
Spacing448448448
CellA=B=C: 313.6 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.70.70.7
M x/y/z448448448
origin x/y/z0.0000.0000.000
length x/y/z313.600313.600313.600
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ448448448
MAP C/R/S321
start NC/NR/NS000
NC/NR/NS448448448
D min/max/mean-19.14536.510-0.000

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Supplemental data

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Additional map: Density modified using Phenix.Resolve

Fileemd_13703_additional_1.map
AnnotationDensity modified using Phenix.Resolve
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Sharpened using SPHIRE

Fileemd_13703_additional_2.map
AnnotationSharpened using SPHIRE
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_13703_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_13703_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Slo tetramer

EntireName: Slo tetramer
Components
  • Complex: Slo tetramer
    • Protein or peptide: Isoform J of Calcium-activated potassium channel slowpoke
  • Ligand: CALCIUM ION
  • Ligand: MAGNESIUM ION
  • Ligand: (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE
  • Ligand: CHOLESTEROL
  • Ligand: POTASSIUM ION
  • Ligand: (3~{S},6~{R},9~{S},12~{R},15~{S},18~{R},21~{S},24~{R})-4,6,10,16,18,22-hexamethyl-3,9,15,21-tetrakis(2-methylpropyl)-12,24-bis[(4-morpholin-4-ylphenyl)methyl]-1,7,13,19-tetraoxa-4,10,16,22-tetrazacyclotetracosane-2,5,8,11,14,17,20,23-octone

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Supramolecule #1: Slo tetramer

SupramoleculeName: Slo tetramer / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Drosophila melanogaster (fruit fly)
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)

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Macromolecule #1: Isoform J of Calcium-activated potassium channel slowpoke

MacromoleculeName: Isoform J of Calcium-activated potassium channel slowpoke
type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Drosophila melanogaster (fruit fly)
Molecular weightTheoretical: 130.919094 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MASGLIDTNF SSTLANGMSG CDQSTVESLA DDPTDSPFDA DDCLKVRKYW CFLLSSIFTF LAGLLVVLLW RAFAFVCCRK EPDLGPNDP KQKEQKASRN KQEFEGTFMT EAKDWAGELI SGQTTTGRIL VVLVFILSIA SLIIYFVDAS SEEVERCQKW S NNITQQID ...String:
MASGLIDTNF SSTLANGMSG CDQSTVESLA DDPTDSPFDA DDCLKVRKYW CFLLSSIFTF LAGLLVVLLW RAFAFVCCRK EPDLGPNDP KQKEQKASRN KQEFEGTFMT EAKDWAGELI SGQTTTGRIL VVLVFILSIA SLIIYFVDAS SEEVERCQKW S NNITQQID LAFNIFFMVY FFIRFIAASD KLWFMLEMYS FVDYFTIPPS FVSIYLDRTW IGLRFLRALR LMTVPDILQY LN VLKTSSS IRLAQLVSIF ISVWLTAAGI IHLLENSGDP LDFDNAHRLS YWTCVYFLIV TMSTVGYGDV YCETVLGRTF LVF FLLVGL AIFASCIPEI IDLIGTRAKY GGTLKNEKGR RHIVVCGHIT YESVSHFLKD FLHEDREDVD VEVVFLHRKP PDLE LEGLF KRHFTTVEFF QGTIMNPIDL QRVKVHEADA CLVLANKYCQ DPDAEDAANI MRVISIKNYS DDIRVIIQLM QYHNK AYLL NIPSWDWKQG DDVICLAELK LGFIAQSCLA PGFSTMMANL FAMRSFKTSP DMQSWTNDYL RGTGMEMYTE TLSPTF IGI PFAQATELCF SKLKLLLLAI EIKGAEEGAD SKISINPRGA KIQANTQGFF IAQSADEVKR AWFYCKACHE DIKDETL IK KCKCKNLATF RKGVRAVQMV GRASDITRDR EDTNLLNRNV RRPNGTGNGT GGMHHMNNTA AAAAAAAAAG KQVNKVKP T VNVSRQVEGQ VISPSQYNRP TSRSSGTGTQ NQNGGVSLPA GIADDQSKDF DFEKTEMKYD STGMFHWSPA KSLEDCILD RNQAAMTVLN GHVVVCLFAD PDSPLIGLRN LVMPLRASNF HYHELKHVVI VGSVDYIRRE WKMLQNLPKI SVLNGSPLSR ADLRAVNVN LCDMCCILSA KVPSNDDPTL ADKEAILASL NIKAMTFDDT IGVLSQRGPE FDNLSATAGS PIVLQRRGSV Y GANVPMIT ELVNDGNVQF LDQDDDDDPD TELYLTQPFA CGTAFAVSVL DSLMSTTYFN QNALTLIRSL ITGGATPELE LI LAEGAGL RGGYSTVESL SNRDRCRVGQ ISLYDGPLAQ FGECGKYGDL FVAALKSYGM LCIGLYRFRD TSSSCDASSK RYV ITNPPD DFSLLPTDQV FVLMQFDPGL EYKPPAVRAP AGGRGTNTQG SGVGGGGSNK DDNS

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Macromolecule #2: CALCIUM ION

MacromoleculeName: CALCIUM ION / type: ligand / ID: 2 / Number of copies: 8 / Formula: CA
Molecular weightTheoretical: 40.078 Da

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Macromolecule #3: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 3 / Number of copies: 4 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #4: (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-...

MacromoleculeName: (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE
type: ligand / ID: 4 / Number of copies: 16 / Formula: 6PL
Molecular weightTheoretical: 763.1 Da
Chemical component information

ChemComp-6PL:
(4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE / phospholipid*YM

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Macromolecule #5: CHOLESTEROL

MacromoleculeName: CHOLESTEROL / type: ligand / ID: 5 / Number of copies: 4 / Formula: CLR
Molecular weightTheoretical: 386.654 Da
Chemical component information

ChemComp-CLR:
CHOLESTEROL

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Macromolecule #6: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 6 / Number of copies: 6 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #7: (3~{S},6~{R},9~{S},12~{R},15~{S},18~{R},21~{S},24~{R})-4,6,10,16,...

MacromoleculeName: (3~{S},6~{R},9~{S},12~{R},15~{S},18~{R},21~{S},24~{R})-4,6,10,16,18,22-hexamethyl-3,9,15,21-tetrakis(2-methylpropyl)-12,24-bis[(4-morpholin-4-ylphenyl)methyl]-1,7,13,19-tetraoxa-4,10,16,22- ...Name: (3~{S},6~{R},9~{S},12~{R},15~{S},18~{R},21~{S},24~{R})-4,6,10,16,18,22-hexamethyl-3,9,15,21-tetrakis(2-methylpropyl)-12,24-bis[(4-morpholin-4-ylphenyl)methyl]-1,7,13,19-tetraoxa-4,10,16,22-tetrazacyclotetracosane-2,5,8,11,14,17,20,23-octone
type: ligand / ID: 7 / Number of copies: 1 / Formula: 8I2
Molecular weightTheoretical: 1.119388 KDa
Chemical component information

ChemComp-8I2:
(3~{S},6~{R},9~{S},12~{R},15~{S},18~{R},21~{S},24~{R})-4,6,10,16,18,22-hexamethyl-3,9,15,21-tetrakis(2-methylpropyl)-12,24-bis[(4-morpholin-4-ylphenyl)methyl]-1,7,13,19-tetraoxa-4,10,16,22-tetrazacyclotetracosane-2,5,8,11,14,17,20,23-octone / antiparasitic*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 75.4 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1861584
CTF correctionSoftware - Name: CTFFIND (ver. 4)
Final reconstructionApplied symmetry - Point group: C2 (2 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 2.59 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: SPHIRE (ver. 1.3) / Number images used: 261505
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: SPHIRE (ver. 1.3)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software: (Name: SPHIRE (ver. 1.3), RELION (ver. 3.1))
Final 3D classificationSoftware - Name: SPHIRE (ver. 1.3)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

5tj6

Output model

PDB-7pxh:
Emodepside-bound Drosophila Slo channel

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