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- EMDB-13494: H. sapiens replisome-CUL2/LRR1 complex -

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Basic information

Entry
Database: EMDB / ID: EMD-13494
TitleH. sapiens replisome-CUL2/LRR1 complex
Map dataLocally filtered consensus refinement
Sample
  • Complex: Human replisome engaged with CUL2-LRR1
    • Protein or peptide: x 22 types
    • DNA: x 2 types
  • Ligand: x 4 types
KeywordsGenome stability / DNA replication / Ubiquitination / termination / replisome / cryo-EM / CMG / Cul2LRR1 / REPLICATION / DNA / polymerase / helicase
Function / homology
Function and homology information


cellular response to bleomycin / DNA secondary structure binding / Switching of origins to a post-replicative state / detection of abiotic stimulus / replication fork arrest / regulation of nuclear cell cycle DNA replication / Unwinding of DNA / DNA replication initiation / epsilon DNA polymerase complex / cell cycle phase transition ...cellular response to bleomycin / DNA secondary structure binding / Switching of origins to a post-replicative state / detection of abiotic stimulus / replication fork arrest / regulation of nuclear cell cycle DNA replication / Unwinding of DNA / DNA replication initiation / epsilon DNA polymerase complex / cell cycle phase transition / cellular response to cisplatin / GINS complex / DNA strand elongation involved in mitotic DNA replication / mitotic DNA replication preinitiation complex assembly / nuclear origin of replication recognition complex / cellular response to hydroxyurea / alpha DNA polymerase:primase complex / mitotic DNA replication / cullin-RING-type E3 NEDD8 transferase / anaphase-promoting complex binding / cellular response to chemical stress / NEDD8 transferase activity / CMG complex / cullin-RING ubiquitin ligase complex / DNA replication checkpoint signaling / single-stranded DNA 3'-5' DNA exonuclease activity / entrainment of circadian clock / single-stranded 3'-5' DNA helicase activity / Cul7-RING ubiquitin ligase complex / ubiquitin-dependent protein catabolic process via the C-end degron rule pathway / regulation of phosphorylation / MCM complex / DNA replication preinitiation complex / Loss of Function of FBXW7 in Cancer and NOTCH1 Signaling / target-directed miRNA degradation / elongin complex / VCB complex / replication fork protection complex / positive regulation of protein autoubiquitination / mitotic DNA replication checkpoint signaling / mitotic DNA replication initiation / protein neddylation / double-strand break repair via break-induced replication / mitotic intra-S DNA damage checkpoint signaling / NEDD8 ligase activity / positive regulation of double-strand break repair / Cul5-RING ubiquitin ligase complex / negative regulation of response to oxidative stress / Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters / regulation of DNA-templated DNA replication initiation / ubiquitin-ubiquitin ligase activity / Cul4A-RING E3 ubiquitin ligase complex / single-stranded DNA helicase activity / SCF ubiquitin ligase complex / inner cell mass cell proliferation / Cul2-RING ubiquitin ligase complex / negative regulation of type I interferon production / nucleotide-excision repair, DNA gap filling / DNA replication proofreading / Cul4B-RING E3 ubiquitin ligase complex / ubiquitin ligase complex scaffold activity / DNA strand elongation involved in DNA replication / SCF-dependent proteasomal ubiquitin-dependent protein catabolic process / Cul3-RING ubiquitin ligase complex / branching morphogenesis of an epithelial tube / DNA synthesis involved in DNA repair / cochlea development / G1/S-Specific Transcription / activation of protein kinase activity / leading strand elongation / replication fork processing / Prolactin receptor signaling / Apoptotic cleavage of cellular proteins / DNA unwinding involved in DNA replication / nuclear replication fork / protein monoubiquitination / mitotic G2 DNA damage checkpoint signaling / 3'-5' DNA helicase activity / DNA replication origin binding / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / cullin family protein binding / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / positive regulation of double-strand break repair via homologous recombination / Activation of the pre-replicative complex / PCNA-Dependent Long Patch Base Excision Repair / DNA replication initiation / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / cellular response to interleukin-4 / embryonic organ development / Nuclear events stimulated by ALK signaling in cancer / protein K48-linked ubiquitination / Formation of HIV elongation complex in the absence of HIV Tat / error-prone translesion synthesis / Activation of ATR in response to replication stress / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / response to UV
Similarity search - Function
DNA polymerase epsilon subunit B, N-terminal / DNA polymerases epsilon N terminal / Claspin / Timeless, C-terminal / Timeless PAB domain / Chromosome segregation in meiosis protein 3 / TIPIN/Csm3/Swi3 / Replication Fork Protection Component Swi3 / Timeless / Timeless, N-terminal ...DNA polymerase epsilon subunit B, N-terminal / DNA polymerases epsilon N terminal / Claspin / Timeless, C-terminal / Timeless PAB domain / Chromosome segregation in meiosis protein 3 / TIPIN/Csm3/Swi3 / Replication Fork Protection Component Swi3 / Timeless / Timeless, N-terminal / Timeless protein / DNA polymerase epsilon, subunit B / : / DNA polymerase alpha-binding protein Ctf4, C-terminal domain / Minichromosome loss protein Mcl1, middle region / Minichromosome loss protein, Mcl1, middle region / DNA polymerase epsilon, catalytic subunit A, C-terminal / DNA polymerase epsilon catalytic subunit / Domain of unknown function (DUF1744) / DUF1744 / CDC45 family / DNA replication complex GINS protein Psf2 / CDC45-like protein / GINS complex, subunit Psf1 / GINS complex, subunit Psf3 / GINS complex, subunit Psf3 superfamily / DNA replication complex GINS protein SLD5, C-terminal / GINS, helical bundle-like domain superfamily / GINS complex protein Sld5, alpha-helical domain / DNA replication complex GINS protein SLD5 C-terminus / GINS complex subunit Sld5 / GINS subunit, domain A / GINS complex protein helical bundle domain / Leucine rich repeat 4 / Leucine Rich repeats (2 copies) / MCM4, winged helix domain / DNA polymerase alpha/delta/epsilon, subunit B / DNA replication licensing factor Mcm5 / DNA polymerase alpha/epsilon subunit B / DNA replication licensing factor Mcm3 / Mini-chromosome maintenance complex protein 4 / DNA replication licensing factor Mcm6 / DNA replication licensing factor Mcm7 / Mcm6, C-terminal winged-helix domain / MCM6 C-terminal winged-helix domain / DNA replication licensing factor Mcm2 / Mini-chromosome maintenance protein 2 / Mini-chromosome maintenance, conserved site / MCM family signature. / Zinc finger, RING-H2-type / RING-H2 zinc finger domain / MCM N-terminal domain / MCM N-terminal domain / Cullin protein neddylation domain / MCM OB domain / MCM OB domain / Mini-chromosome maintenance protein / MCM, AAA-lid domain / MCM P-loop domain / MCM AAA-lid domain / MCM family domain profile. / minichromosome maintenance proteins / MCM domain / Anaphase-promoting complex subunit 4, WD40 domain / Cullin, conserved site / Cullin family signature. / Elongin B / Cullin / Elongin-C / Cullin repeat-like-containing domain superfamily / Cullin protein, neddylation domain / Cullin protein neddylation domain / Anaphase-promoting complex subunit 4 WD40 domain / Cullin / Cullin, N-terminal / Cullin homology domain / Cullin homology domain superfamily / Cullin family / Cullin family profile. / DNA polymerase family B, thumb domain / HMG boxes A and B DNA-binding domains profile. / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain / Skp1 family, tetramerisation domain / Found in Skp1 protein family / high mobility group / High mobility group box domain / High mobility group box domain superfamily / DNA polymerase family B / DNA polymerase family B, exonuclease domain / DNA-directed DNA polymerase, family B, exonuclease domain / DNA-directed DNA polymerase, family B, multifunctional domain / DNA polymerase, palm domain superfamily / DNA polymerase type-B family / DNA-directed DNA polymerase, family B / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily / Leucine-rich repeat profile. / SKP1/BTB/POZ domain superfamily / Leucine-rich repeat
Similarity search - Domain/homology
Cell division control protein 45 homolog / WD repeat and HMG-box DNA-binding protein 1 / DNA replication licensing factor MCM3 / DNA replication licensing factor MCM4 / DNA replication licensing factor MCM5 / DNA replication licensing factor MCM7 / DNA replication licensing factor MCM2 / DNA polymerase epsilon subunit 2 / E3 ubiquitin-protein ligase RBX1 / DNA polymerase epsilon catalytic subunit A ...Cell division control protein 45 homolog / WD repeat and HMG-box DNA-binding protein 1 / DNA replication licensing factor MCM3 / DNA replication licensing factor MCM4 / DNA replication licensing factor MCM5 / DNA replication licensing factor MCM7 / DNA replication licensing factor MCM2 / DNA polymerase epsilon subunit 2 / E3 ubiquitin-protein ligase RBX1 / DNA polymerase epsilon catalytic subunit A / Cullin-2 / DNA replication licensing factor MCM6 / DNA replication complex GINS protein PSF1 / Elongin-C / Elongin-B / Leucine-rich repeat protein 1 / DNA replication complex GINS protein SLD5 / DNA replication complex GINS protein PSF3 / TIMELESS-interacting protein / Claspin / Protein timeless homolog / DNA replication complex GINS protein PSF2
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsJones MJ / Yeeles JTP
Funding support United Kingdom, 3 items
OrganizationGrant numberCountry
Medical Research Council (MRC, United Kingdom)MC_UP_1201/12 United Kingdom
Medical Research Council (MRC, United Kingdom)MC_UU_12016/13 United Kingdom
Wellcome Trust204678/Z/16/Z United Kingdom
CitationJournal: Nature / Year: 2021
Title: A conserved mechanism for regulating replisome disassembly in eukaryotes.
Authors: Michael Jenkyn-Bedford / Morgan L Jones / Yasemin Baris / Karim P M Labib / Giuseppe Cannone / Joseph T P Yeeles / Tom D Deegan /
Abstract: Replisome disassembly is the final step of eukaryotic DNA replication and is triggered by ubiquitylation of the CDC45-MCM-GINS (CMG) replicative helicase. Despite being driven by evolutionarily ...Replisome disassembly is the final step of eukaryotic DNA replication and is triggered by ubiquitylation of the CDC45-MCM-GINS (CMG) replicative helicase. Despite being driven by evolutionarily diverse E3 ubiquitin ligases in different eukaryotes (SCF in budding yeast, CUL2 in metazoa), replisome disassembly is governed by a common regulatory principle, in which ubiquitylation of CMG is suppressed before replication termination, to prevent replication fork collapse. Recent evidence suggests that this suppression is mediated by replication fork DNA. However, it is unknown how SCF and CUL2 discriminate terminated from elongating replisomes, to selectively ubiquitylate CMG only after termination. Here we used cryo-electron microscopy to solve high-resolution structures of budding yeast and human replisome-E3 ligase assemblies. Our structures show that the leucine-rich repeat domains of Dia2 and LRR1 are structurally distinct, but bind to a common site on CMG, including the MCM3 and MCM5 zinc-finger domains. The LRR-MCM interaction is essential for replisome disassembly and, crucially, is occluded by the excluded DNA strand at replication forks, establishing the structural basis for the suppression of CMG ubiquitylation before termination. Our results elucidate a conserved mechanism for the regulation of replisome disassembly in eukaryotes, and reveal a previously unanticipated role for DNA in preserving replisome integrity.
History
DepositionSep 1, 2021-
Header (metadata) releaseNov 10, 2021-
Map releaseNov 10, 2021-
UpdateOct 18, 2023-
Current statusOct 18, 2023Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0181
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.0181
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7plo
  • Surface level: 0.0181
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_13494.png

Downloads & links

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Map

FileDownload / File: emd_13494.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationLocally filtered consensus refinement
Projections & slices

Image control

Size
Brightness
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AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 400 pix.
= 428.8 Å		1.07 Å/pix.
x 400 pix.
= 428.8 Å		1.07 Å/pix.
x 400 pix.
= 428.8 Å

Surface

Projections

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.072 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0181 / Movie #1: 0.0181
Minimum - Maximum-0.17981409 - 0.33572605
Average (Standard dev.)0.0007124531 (±0.006124998)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 428.80002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0721.0721.072
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z428.800428.800428.800
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ440440440
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.1800.3360.001

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Supplemental data

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Mask #1

Fileemd_13494_msk_1.map
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Additional map: consensus refinement

Fileemd_13494_additional_1.map
Annotationconsensus refinement
Projections & Slices
AxesZYX

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Additional map: consensus refinement sharpened

Fileemd_13494_additional_2.map
Annotationconsensus refinement sharpened
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AxesZYX

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Half map: consensus refinement half 1

Fileemd_13494_half_map_1.map
Annotationconsensus refinement half 1
Projections & Slices
AxesZYX

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Histogram (log scale)

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Half map: consensus refinement half 2

Fileemd_13494_half_map_2.map
Annotationconsensus refinement half 2
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AxesZYX

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Sample components

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Entire : Human replisome engaged with CUL2-LRR1

EntireName: Human replisome engaged with CUL2-LRR1
Components
  • Complex: Human replisome engaged with CUL2-LRR1
    • Protein or peptide: Claspin
    • Protein or peptide: DNA replication licensing factor MCM2
    • Protein or peptide: DNA replication licensing factor MCM3
    • Protein or peptide: DNA replication licensing factor MCM4
    • Protein or peptide: DNA replication licensing factor MCM5
    • Protein or peptide: DNA replication licensing factor MCM6
    • Protein or peptide: DNA replication licensing factor MCM7
    • Protein or peptide: DNA polymerase epsilon subunit 2
    • Protein or peptide: DNA polymerase epsilon catalytic subunit A
    • Protein or peptide: Cell division control protein 45 homolog
    • Protein or peptide: DNA replication complex GINS protein PSF1
    • Protein or peptide: DNA replication complex GINS protein PSF2
    • Protein or peptide: DNA replication complex GINS protein PSF3
    • Protein or peptide: DNA replication complex GINS protein SLD5
    • Protein or peptide: WD repeat and HMG-box DNA-binding protein 1
    • Protein or peptide: Protein timeless homolog
    • Protein or peptide: TIMELESS-interacting protein
    • DNA: Leading strand DNA
    • DNA: Lagging strand DNA
    • Protein or peptide: Leucine-rich repeat protein 1
    • Protein or peptide: Elongin-B
    • Protein or peptide: Elongin-C
    • Protein or peptide: Cullin-2
    • Protein or peptide: E3 ubiquitin-protein ligase RBX1
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
  • Ligand: SULFATE ION

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Supramolecule #1: Human replisome engaged with CUL2-LRR1

SupramoleculeName: Human replisome engaged with CUL2-LRR1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #21, #1-#20, #22-#24
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: DNA replication licensing factor MCM2

MacromoleculeName: DNA replication licensing factor MCM2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 102.034102 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAESSESFTM ASSPAQRRRG NDPLTSSPGR SSRRTDALTS SPGRDLPPFE DESEGLLGTE GPLEEEEDGE ELIGDGMERD YRAIPELDA YEAEGLALDD EDVEELTASQ REAAERAMRQ RDREAGRGLG RMRRGLLYDS DEEDEERPAR KRRQVERATE D GEEDEEMI ...String:
MAESSESFTM ASSPAQRRRG NDPLTSSPGR SSRRTDALTS SPGRDLPPFE DESEGLLGTE GPLEEEEDGE ELIGDGMERD YRAIPELDA YEAEGLALDD EDVEELTASQ REAAERAMRQ RDREAGRGLG RMRRGLLYDS DEEDEERPAR KRRQVERATE D GEEDEEMI ESIENLEDLK GHSVREWVSM AGPRLEIHHR FKNFLRTHVD SHGHNVFKER ISDMCKENRE SLVVNYEDLA AR EHVLAYF LPEAPAELLQ IFDEAALEVV LAMYPKYDRI TNHIHVRISH LPLVEELRSL RQLHLNQLIR TSGVVTSCTG VLP QLSMVK YNCNKCNFVL GPFCQSQNQE VKPGSCPECQ SAGPFEVNME ETIYQNYQRI RIQESPGKVA AGRLPRSKDA ILLA DLVDS CKPGDEIELT GIYHNNYDGS LNTANGFPVF ATVILANHVA KKDNKVAVGE LTDEDVKMIT SLSKDQQIGE KIFAS IAPS IYGHEDIKRG LALALFGGEP KNPGGKHKVR GDINVLLCGD PGTAKSQFLK YIEKVSSRAI FTTGQGASAV GLTAYV QRH PVSREWTLEA GALVLADRGV CLIDEFDKMN DQDRTSIHEA MEQQSISISK AGIVTSLQAR CTVIAAANPI GGRYDPS LT FSENVDLTEP IISRFDILCV VRDTVDPVQD EMLARFVVGS HVRHHPSNKE EEGLANGSAA EPAMPNTYGV EPLPQEVL K KYIIYAKERV HPKLNQMDQD KVAKMYSDLR KESMATGSIP ITVRHIESMI RMAEAHARIH LRDYVIEDDV NMAIRVMLE SFIDTQKFSV MRSMRKTFAR YLSFRRDNNE LLLFILKQLV AEQVTYQRNR FGAQQDTIEV PEKDLVDKAR QINIHNLSAF YDSELFRMN KFSHDLKRKM ILQQF

UniProtKB: DNA replication licensing factor MCM2

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Macromolecule #2: DNA replication licensing factor MCM3

MacromoleculeName: DNA replication licensing factor MCM3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 91.110852 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQSKVRELI SDNQYRLIVN VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDA TYAKQYEEFY VGLEGSFGSK HVSPRTLTSC FLSCVVCVEG IVTKCSLVRP KVVRSVHYCP ATKKTIERRY S DLTTLVAF ...String:
MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQSKVRELI SDNQYRLIVN VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDA TYAKQYEEFY VGLEGSFGSK HVSPRTLTSC FLSCVVCVEG IVTKCSLVRP KVVRSVHYCP ATKKTIERRY S DLTTLVAF PSSSVYPTKD EENNPLETEY GLSVYKDHQT ITIQEMPEKA PAGQLPRSVD VILDDDLVDK AKPGDRVQVV GT YRCLPGK KGGYTSGTFR TVLIACNVKQ MSKDAQPSFS AEDIAKIKKF SKTRSKDIFD QLAKSLAPSI HGHDYVKKAI LCL LLGGVE RDLENGSHIR GDINILLIGD PSVAKSQLLR YVLCTAPRAI PTTGRGSSGV GLTAAVTTDQ ETGERRLEAG AMVL ADRGV VCIDEFDKMS DMDRTAIHEV MEQGRVTIAK AGIHARLNAR CSVLAAANPV YGRYDQYKTP MENIGLQDSL LSRFD LLFI MLDQMDPEQD REISDHVLRM HRYRAPGEQD GDAMPLGSAV DILATDDPNF SQEDQQDTQI YEKHDNLLHG TKKKKE KMV SAAFMKKYIH VAKIIKPVLT QESATYIAEE YSRLRSQDSM SSDTARTSPV TARTLETLIR LATAHAKARM SKTVDLQ DA EEAVELVQYA YFKKVLEKEK KRKKRSEDES ETEDEEEKSQ EDQEQKRKRR KTRQPDAKDG DSYDPYDFSD TEEEMPQV H TPKTADSQET KESQKVELSE SRLKAFKVAL LDVFREAHAQ SIGMNRLTES INRDSEEPFS SVEIQAALSK MQDDNQVMV SEGIIFLI

UniProtKB: DNA replication licensing factor MCM3

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Macromolecule #3: DNA replication licensing factor MCM4

MacromoleculeName: DNA replication licensing factor MCM4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 96.684852 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSSPASTPSR RGSRRGRATP AQTPRSEDAR SSPSQRRRGE DSTSTGELQP MPTSPGVDLQ SPAAQDVLFS SPPQMHSSAI PLDFDVSSP LTYGTPSSRV EGTPRSGVRG TPVRQRPDLG SAQKGLQVDL QSDGAAAEDI VASEQSLGQK LVIWGTDVNV A ACKENFQR ...String:
MSSPASTPSR RGSRRGRATP AQTPRSEDAR SSPSQRRRGE DSTSTGELQP MPTSPGVDLQ SPAAQDVLFS SPPQMHSSAI PLDFDVSSP LTYGTPSSRV EGTPRSGVRG TPVRQRPDLG SAQKGLQVDL QSDGAAAEDI VASEQSLGQK LVIWGTDVNV A ACKENFQR FLQRFIDPLA KEEENVGIDI TEPLYMQRLG EINVIGEPFL NVNCEHIKSF DKNLYRQLIS YPQEVIPTFD MA VNEIFFD RYPDSILEHQ IQVRPFNALK TKNMRNLNPE DIDQLITISG MVIRTSQLIP EMQEAFFQCQ VCAHTTRVEM DRG RIAEPS VCGRCHTTHS MALIHNRSLF SDKQMIKLQE SPEDMPAGQT PHTVILFAHN DLVDKVQPGD RVNVTGIYRA VPIR VNPRV SNVKSVYKTH IDVIHYRKTD AKRLHGLDEE AEQKLFSEKR VELLKELSRK PDIYERLASA LAPSIYEHED IKKGI LLQL FGGTRKDFSH TGRGKFRAEI NILLCGDPGT SKSQLLQYVY NLVPRGQYTS GKGSSAVGLT AYVMKDPETR QLVLQT GAL VLSDNGICCI DEFDKMNEST RSVLHEVMEQ QTLSIAKAGI ICQLNARTSV LAAANPIESQ WNPKKTTIEN IQLPHTL LS RFDLIFLLLD PQDEAYDRRL AHHLVALYYQ SEEQAEEELL DMAVLKDYIA YAHSTIMPRL SEEASQALIE AYVDMRKI G SSRGMVSAYP RQLESLIRLA EAHAKVRLSN KVEAIDVEEA KRLHREALKQ SATDPRTGIV DISILTTGMS ATSRKRKEE LAEALKKLIL SKGKTPALKY QQLFEDIRGQ SDIAITKDMF EEALRALADD DFLTVTGKTV RLL

UniProtKB: DNA replication licensing factor MCM4

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Macromolecule #4: DNA replication licensing factor MCM5

MacromoleculeName: DNA replication licensing factor MCM5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 82.406633 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEDLADYLY KQPAEHLQLL EEAAKEVADE VTRPRPSGEE VLQDIQVMLK SDASPSSIRS LKSDMMSHLV KIPGIIIAAS A VRAKATRI ...String:
MSGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEDLADYLY KQPAEHLQLL EEAAKEVADE VTRPRPSGEE VLQDIQVMLK SDASPSSIRS LKSDMMSHLV KIPGIIIAAS A VRAKATRI SIQCRSCRNT LTNIAMRPGL EGYALPRKCN TDQAGRPKCP LDPYFIMPDK CKCVDFQTLK LQELPDAVPH GE MPRHMQL YCDRYLCDKV VPGNRVTIMG IYSIKKFGLT TSRGRDRVGV GIRSSYIRVL GIQVDTDGSG RSFAGAVSPQ EEE EFRRLA ALPNVYEVIS KSIAPSIFGG TDMKKAIACL LFGGSRKRLP DGLTRRGDIN LLMLGDPGTA KSQLLKFVEK CSPI GVYTS GKGSSAAGLT ASVMRDPSSR NFIMEGGAMV LADGGVVCID EFDKMREDDR VAIHEAMEQQ TISIAKAGIT TTLNS RCSV LAAANSVFGR WDETKGEDNI DFMPTILSRF DMIFIVKDEH NEERDVMLAK HVITLHVSAL TQTQAVEGEI DLAKLK KFI AYCRVKCGPR LSAEAAEKLK NRYIIMRSGA RQHERDSDRR SSIPITVRQL EAIVRIAEAL SKMKLQPFAT EADVEEA LR LFQVSTLDAA LSGTLSGVEG FTSQEDQEML SRIEKQLKRR FAIGSQVSEH SIIKDFTKQK YPEHAIHKVL QLMLRRGE I QHRMQRKVLY RLK

UniProtKB: DNA replication licensing factor MCM5

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Macromolecule #5: DNA replication licensing factor MCM6

MacromoleculeName: DNA replication licensing factor MCM6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 93.010273 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MDLAAAAEPG AGSQHLEVRD EVAEKCQKLF LDFLEEFQSS DGEIKYLQLA EELIRPERNT LVVSFVDLEQ FNQQLSTTIQ EEFYRVYPY LCRALKTFVK DRKEIPLAKD FYVAFQDLPT RHKIRELTSS RIGLLTRISG QVVRTHPVHP ELVSGTFLCL D CQTVIRDV ...String:
MDLAAAAEPG AGSQHLEVRD EVAEKCQKLF LDFLEEFQSS DGEIKYLQLA EELIRPERNT LVVSFVDLEQ FNQQLSTTIQ EEFYRVYPY LCRALKTFVK DRKEIPLAKD FYVAFQDLPT RHKIRELTSS RIGLLTRISG QVVRTHPVHP ELVSGTFLCL D CQTVIRDV EQQFKYTQPN ICRNPVCANR RRFLLDTNKS RFVDFQKVRI QETQAELPRG SIPRSLEVIL RAEAVESAQA GD KCDFTGT LIVVPDVSKL STPGARAETN SRVSGVDGYE TEGIRGLRAL GVRDLSYRLV FLACCVAPTN PRFGGKELRD EEQ TAESIK NQMTVKEWEK VFEMSQDKNL YHNLCTSLFP TIHGNDEVKR GVLLMLFGGV PKTTGEGTSL RGDINVCIVG DPST AKSQF LKHVEEFSPR AVYTSGKASS AAGLTAAVVR DEESHEFVIE AGALMLADNG VCCIDEFDKM DVRDQVAIHE AMEQQ TISI TKAGVKATLN ARTSILAAAN PISGHYDRSK SLKQNINLSA PIMSRFDLFF ILVDECNEVT DYAIARRIVD LHSRIE ESI DRVYSLDDIR RYLLFARQFK PKISKESEDF IVEQYKHLRQ RDGSGVTKSS WRITVRQLES MIRLSEAMAR MHCCDEV QP KHVKEAFRLL NKSIIRVETP DVNLDQEEEI QMEVDEGAGG INGHADSPAP VNGINGYNED INQESAPKAS LRLGFSEY C RISNLIVLHL RKVEEEEDES ALKRSELVNW YLKEIESEID SEEELINKKR IIEKVIHRLT HYDHVLIELT QAGLKGSTE GSESYEEDPY LVVNPNYLLE D

UniProtKB: DNA replication licensing factor MCM6

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Macromolecule #6: DNA replication licensing factor MCM7

MacromoleculeName: DNA replication licensing factor MCM7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 81.411875 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MALKDYALEK EKVKKFLQEF YQDDELGKKQ FKYGNQLVRL AHREQVALYV DLDDVAEDDP ELVDSICENA RRYAKLFADA VQELLPQYK EREVVNKDVL DVYIEHRLMM EQRSRDPGMV RSPQNQYPAE LMRRFELYFQ GPSSNKPRVI REVRADSVGK L VTVRGIVT ...String:
MALKDYALEK EKVKKFLQEF YQDDELGKKQ FKYGNQLVRL AHREQVALYV DLDDVAEDDP ELVDSICENA RRYAKLFADA VQELLPQYK EREVVNKDVL DVYIEHRLMM EQRSRDPGMV RSPQNQYPAE LMRRFELYFQ GPSSNKPRVI REVRADSVGK L VTVRGIVT RVSEVKPKMV VATYTCDQCG AETYQPIQSP TFMPLIMCPS QECQTNRSGG RLYLQTRGSR FIKFQEMKMQ EH SDQVPVG NIPRSITVLV EGENTRIAQP GDHVSVTGIF LPILRTGFRQ VVQGLLSETY LEAHRIVKMN KSEDDESGAG ELT REELRQ IAEEDFYEKL AASIAPEIYG HEDVKKALLL LLVGGVDQSP RGMKIRGNIN ICLMGDPGVA KSQLLSYIDR LAPR SQYTT GRGSSGVGLT AAVLRDSVSG ELTLEGGALV LADQGVCCID EFDKMAEADR TAIHEVMEQQ TISIAKAGIL TTLNA RCSI LAAANPAYGR YNPRRSLEQN IQLPAALLSR FDLLWLIQDR PDRDNDLRLA QHITYVHQHS RQPPSQFEPL DMKLMR RYI AMCREKQPMV PESLADYITA AYVEMRREAW ASKDATYTSA RTLLAILRLS TALARLRMVD VVEKEDVNEA IRLMEMS KD SLLGDKGQTA RTQRPADVIF ATVRELVSGG RSVRFSEAEQ RCVSRGFTPA QFQAALDEYE ELNVWQVNAS RTRITFV

UniProtKB: DNA replication licensing factor MCM7

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Macromolecule #7: DNA polymerase epsilon subunit 2

MacromoleculeName: DNA polymerase epsilon subunit 2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 59.600887 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN AVEKQPLSSN MIERSVVEAA VQECSQSVDE TIEHVFNII GAFDIPRFVY NSERKKFLPL LMTNHPAPNL FGTPRDKAEM FRERYTILHQ RTHRHELFTP PVIGSHPDES G SKFQLKTI ...String:
MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN AVEKQPLSSN MIERSVVEAA VQECSQSVDE TIEHVFNII GAFDIPRFVY NSERKKFLPL LMTNHPAPNL FGTPRDKAEM FRERYTILHQ RTHRHELFTP PVIGSHPDES G SKFQLKTI ETLLGSTTKI GDAIVLGMIT QLKEGKFFLE DPTGTVQLDL SKAQFHSGLY TEACFVLAEG WFEDQVFHVN AF GFPPTEP SSTTRAYYGN INFFGGPSNT SVKTSAKLKQ LEEENKDAMF VFLSDVWLDQ VEVLEKLRIM FAGYSPAPPT CFI LCGNFS SAPYGKNQVQ ALKDSLKTLA DIICEYPDIH QSSRFVFVPG PEDPGFGSIL PRPPLAESIT NEFRQRVPFS VFTT NPCRI QYCTQEITVF REDLVNKMCR NCVRFPSSNL AIPNHFVKTI LSQGHLTPLP LYVCPVYWAY DYALRVYPVP DLLVI ADKY DPFTTTNTEC LCINPGSFPR SGFSFKVFYP SNKTVEDSKL QGF

UniProtKB: DNA polymerase epsilon subunit 2

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Macromolecule #8: DNA polymerase epsilon catalytic subunit A

MacromoleculeName: DNA polymerase epsilon catalytic subunit A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed DNA polymerase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 261.855266 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSLRSGGRRR ADPGADGEAS RDDGATSSVS ALKRLERSQW TDKMDLRFGF ERLKEPGEKT GWLINMHPTE ILDEDKRLGS AVDYYFIQD DGSRFKVALP YKPYFYIATR KGCEREVSSF LSKKFQGKIA KVETVPKEDL DLPNHLVGLK RNYIRLSFHT V EDLVKVRK ...String:
MSLRSGGRRR ADPGADGEAS RDDGATSSVS ALKRLERSQW TDKMDLRFGF ERLKEPGEKT GWLINMHPTE ILDEDKRLGS AVDYYFIQD DGSRFKVALP YKPYFYIATR KGCEREVSSF LSKKFQGKIA KVETVPKEDL DLPNHLVGLK RNYIRLSFHT V EDLVKVRK EISPAVKKNR EQDHASDAYT ALLSSVLQRG GVITDEEETS KKIADQLDNI VDMREYDVPY HIRLSIDLKI HV AHWYNVR YRGNAFPVEI TRRDDLVERP DPVVLAFDIE TTKLPLKFPD AETDQIMMIS YMIDGQGYLI TNREIVSEDI EDF EFTPKP EYEGPFCVFN EPDEAHLIQR WFEHVQETKP TIMVTYNGDF FDWPFVEARA AVHGLSMQQE IGFQKDSQGE YKAP QCIHM DCLRWVKRDS YLPVGSHNLK AAAKAKLGYD PVELDPEDMC RMATEQPQTL ATYSVSDAVA TYYLYMKYVH PFIFA LCTI IPMEPDEVLR KGSGTLCEAL LMVQAFHANI IFPNKQEQEF NKLTDDGHVL DSETYVGGHV EALESGVFRS DIPCRF RMN PAAFDFLLQR VEKTLRHALE EEEKVPVEQV TNFEEVCDEI KSKLASLKDV PSRIECPLIY HLDVGAMYPN IILTNRL QP SAMVDEATCA ACDFNKPGAN CQRKMAWQWR GEFMPASRSE YHRIQHQLES EKFPPLFPEG PARAFHELSR EEQAKYEK R RLADYCRKAY KKIHITKVEE RLTTICQREN SFYVDTVRAF RDRRYEFKGL HKVWKKKLSA AVEVGDAAEV KRCKNMEVL YDSLQLAHKC ILNSFYGYVM RKGARWYSME MAGIVCFTGA NIITQARELI EQIGRPLELD TDGIWCVLPN SFPENFVFKT TNVKKPKVT ISYPGAMLNI MVKEGFTNDQ YQELAEPSSL TYVTRSENSI FFEVDGPYLA MILPASKEEG KKLKKRYAVF N EDGSLAEL KGFEVKRRGE LQLIKIFQSS VFEAFLKGST LEEVYGSVAK VADYWLDVLY SKAANMPDSE LFELISENRS MS RKLEDYG EQKSTSISTA KRLAEFLGDQ MVKDAGLSCR YIISRKPEGS PVTERAIPLA IFQAEPTVRK HFLRKWLKSS SLQ DFDIRA ILDWDYYIER LGSAIQKIIT IPAALQQVKN PVPRVKHPDW LHKKLLEKND VYKQKKISEL FTLEGRRQVT MAEA SEDSP RPSAPDMEDF GLVKLPHPAA PVTVKRKRVL WESQEESQDL TPTVPWQEIL GQPPALGTSQ EEWLVWLRFH KKKWQ LQAR QRLARRKRQR LESAEGVLRP GAIRDGPATG LGSFLRRTAR SILDLPWQIV QISETSQAGL FRLWALVGSD LHCIRL SIP RVFYVNQRVA KAEEGASYRK VNRVLPRSNM VYNLYEYSVP EDMYQEHINE INAELSAPDI EGVYETQVPL LFRALVH LG CVCVVNKQLV RHLSGWEAET FALEHLEMRS LAQFSYLEPG SIRHIYLYHH AQAHKALFGI FIPSQRRASV FVLDTVRS N QMPSLGALYS AEHGLLLEKV GPELLPPPKH TFEVRAETDL KTICRAIQRF LLAYKEERRG PTLIAVQSSW ELKRLASEI PVLEEFPLVP ICVADKINYG VLDWQRHGAR RMIRHYLNLD TCLSQAFEMS RYFHIPIGNL PEDISTFGSD LFFARHLQRH NHLLWLSPT ARPDLGGKEA DDNCLVMEFD DQATVEINSS GCYSTVCVEL DLQNLAVNTI LQSHHVNDME GADSMGISFD V IQQASLED MITGGQAASA PASYDETALC SNTFRILKSM VVGWVKEITQ YHNIYADNQV MHFYRWLRSP SSLLHDPALH RT LHNMMKK LFLQLIAEFK RLGSSVIYAN FNRIILCTKK RRVEDAIAYV EYITSSIHSK ETFHSLTISF SRCWEFLLWM DPS NYGGIK GKVSSRIHCG LQDSQKAGGA EDEQENEDDE EERDGEEEEE AEESNVEDLL ENNWNILQFL PQAASCQNYF LMIV SAYIV AVYHCMKDGL RRSAPGSTPV RRRGASQLSQ EAEGAVGALP GMITFSQDYV ANELTQSFFT ITQKIQKKVT GSRNS TELS EMFPVLPGSH LLLNNPALEF IKYVCKVLSL DTNITNQVNK LNRDLLRLVD VGEFSEEAQF RDPCRSYVLP EVICRS CNF CRDLDLCKDS SFSEDGAVLP QWLCSNCQAP YDSSAIEMTL VEVLQKKLMA FTLQDLVCLK CRGVKETSMP VYCSCAG DF ALTIHTQVFM EQIGIFRNIA QHYGMSYLLE TLEWLLQKNP QLGH

UniProtKB: DNA polymerase epsilon catalytic subunit A

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Macromolecule #9: Cell division control protein 45 homolog

MacromoleculeName: Cell division control protein 45 homolog / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 66.016891 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MFVSDFRKEF YEVVQSQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET AFLEHKEQFH YFILINCGAN VDLLDILQP DEDTIFFVCD THRPVNVVNV YNDTQIKLLI KQDDDLEVPA YEDIFRDEEE DEEHSGNDSD GSEPSEKRTR L DYKDDDIV ...String:
MFVSDFRKEF YEVVQSQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET AFLEHKEQFH YFILINCGAN VDLLDILQP DEDTIFFVCD THRPVNVVNV YNDTQIKLLI KQDDDLEVPA YEDIFRDEEE DEEHSGNDSD GSEPSEKRTR L DYKDDDIV EQTMRRRQRR EWEARRRDIL FDYEQYEYHG TSSAMVMFEL AWMLSKDLND MLWWAIVGLT DQWVQDKITQ MK YVTDVGV LQRHVSRHNH RNEDEENTLS VDCTRISFEY DLRLVLYQHW SLHDSLCNTS YTAARFKLWS VHGQKRLQEF LAD MGLPLK QVKQKFQAMD ISLKENLREM IEESANKFGM KDMRVQTFSI HFGFKHKFLA SDVVFATMSL MESPEKDGSG TDHF IQALD SLSRSNLDKL YHGLELAKKQ LRATQQTIAS CLCTNLVISQ GPFLYCSLME GTPDVMLFSR PASLSLLSKH LLKSF VCST KNRRCKLLPL VMAAPLSMEH GTVTVVGIPP ETDSSDRKNF FGRAFEKAAE STSSRMLHNH FDLSVIELKA EDRSKF LDA LISLLS

UniProtKB: Cell division control protein 45 homolog, Cell division control protein 45 homolog

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Macromolecule #10: DNA replication complex GINS protein PSF1

MacromoleculeName: DNA replication complex GINS protein PSF1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.022469 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MFCEKAMELI RELHRAPEGQ LPAFNEDGLR QVLEEMKALY EQNQSDVNEA KSGGRSDLIP TIKFRHCSLL RNRRCTVAYL YDRLLRIRA LRWEYGSVLP NALRFHMAAE EMEWFNNYKR SLATYMRSLG GDEGLDITQD MKPPKSLYIE VRCLKDYGEF E VDDGTSVL ...String:
MFCEKAMELI RELHRAPEGQ LPAFNEDGLR QVLEEMKALY EQNQSDVNEA KSGGRSDLIP TIKFRHCSLL RNRRCTVAYL YDRLLRIRA LRWEYGSVLP NALRFHMAAE EMEWFNNYKR SLATYMRSLG GDEGLDITQD MKPPKSLYIE VRCLKDYGEF E VDDGTSVL LKKNSQHFLP RWKCEQLIRQ GVLEHILS

UniProtKB: DNA replication complex GINS protein PSF1

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Macromolecule #11: DNA replication complex GINS protein PSF2

MacromoleculeName: DNA replication complex GINS protein PSF2 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.453713 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString:
MDAAEVEFLA EKELVTIIPN FSLDKIYLIG GDLGPFNPGL PVEVPLWLAI NLKQRQKCRL LPPEWMDVEK LEKMRDHERK EETFTPMPS PYYMELTKLL LNHASDNIPK ADEIRTLVKD MWDTRIAKLR VSADSFVRQQ EAHAKLDNLT LMEINTSGTF L TQALNHMY KLRTNLQPLE STQSQDF

UniProtKB: DNA replication complex GINS protein PSF2

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Macromolecule #12: DNA replication complex GINS protein PSF3

MacromoleculeName: DNA replication complex GINS protein PSF3 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.562611 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSEAYFRVES GALGPEENFL SLDDILMSHE KLPVRTETAM PRLGAFFLER SAGAETDNAV PQGSKLELPL WLAKGLFDNK RRILSVELP KIYQEGWRTV FSADPNVVDL HKMGPHFYGF GSQLLHFDSP ENADISQSLL QTFIGRFRRI MDSSQNAYNE D TSALVARL ...String:
MSEAYFRVES GALGPEENFL SLDDILMSHE KLPVRTETAM PRLGAFFLER SAGAETDNAV PQGSKLELPL WLAKGLFDNK RRILSVELP KIYQEGWRTV FSADPNVVDL HKMGPHFYGF GSQLLHFDSP ENADISQSLL QTFIGRFRRI MDSSQNAYNE D TSALVARL DEMERGLFQT GQKGLNDFQC WEKGQASQIT ASNLVQNYKK RKFTDMED

UniProtKB: DNA replication complex GINS protein PSF3

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Macromolecule #13: DNA replication complex GINS protein SLD5

MacromoleculeName: DNA replication complex GINS protein SLD5 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 30.114236 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MWSHPQFEKG GGSGGGSGGS AWSHPQFEKS GLEVLFQGPM TEEVDFLGQD SDGGSEEVVL TPAELIERLE QAWMNEKFAP ELLESKPEI VECVMEQLEH MEENLRRAKR EDLKVSIHQM EMERIRYVLS SYLRCRLMKI EKFFPHVLEK EKTRPEGEPS S LSPEELAF ...String:
MWSHPQFEKG GGSGGGSGGS AWSHPQFEKS GLEVLFQGPM TEEVDFLGQD SDGGSEEVVL TPAELIERLE QAWMNEKFAP ELLESKPEI VECVMEQLEH MEENLRRAKR EDLKVSIHQM EMERIRYVLS SYLRCRLMKI EKFFPHVLEK EKTRPEGEPS S LSPEELAF AREFMANTES YLKNVALKHM PPNLQKVDLF RAVPKPDLDS YVFLRVRERQ ENILVEPDTD EQRDYVIDLE KG SQHLIRY KTIAPLVASG AVQLI

UniProtKB: DNA replication complex GINS protein SLD5

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Macromolecule #14: WD repeat and HMG-box DNA-binding protein 1

MacromoleculeName: WD repeat and HMG-box DNA-binding protein 1 / type: protein_or_peptide / ID: 14 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 130.098148 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MDYKDDDDKD YKDDDDKDYK DDDDKENLYF QGMPATRKPM RYGHTEGHTE VCFDDSGSFI VTCGSDGDVR IWEDLDDDDP KFINVGEKA YSCALKSGKL VTAVSNNTIQ VHTFPEGVPD GILTRFTTNA NHVVFNGDGT KIAAGSSDFL VKIVDVMDSS Q QKTFRGHD ...String:
MDYKDDDDKD YKDDDDKDYK DDDDKENLYF QGMPATRKPM RYGHTEGHTE VCFDDSGSFI VTCGSDGDVR IWEDLDDDDP KFINVGEKA YSCALKSGKL VTAVSNNTIQ VHTFPEGVPD GILTRFTTNA NHVVFNGDGT KIAAGSSDFL VKIVDVMDSS Q QKTFRGHD APVLSLSFDP KDIFLASASC DGSVRVWQIS DQTCAISWPL LQKCNDVINA KSICRLAWQP KSGKLLAIPV EK SVKLYRR ESWSHQFDLS DNFISQTLNI VTWSPCGQYL AAGSINGLII VWNVETKDCM ERVKHEKGYA ICGLAWHPTC GRI SYTDAE GNLGLLENVC DPSGKTSSSK VSSRVEKDYN DLFDGDDMSN AGDFLNDNAV EIPSFSKGII NDDEDDEDLM MASG RPRQR SHILEDDENS VDISMLKTGS SLLKEEEEDG QEGSIHNLPL VTSQRPFYDG PMPTPRQKPF QSGSTPLHLT HRFMV WNSI GIIRCYNDEQ DNAIDVEFHD TSIHHATHLS NTLNYTIADL SHEAILLACE STDELASKLH CLHFSSWDSS KEWIID LPQ NEDIEAICLG QGWAAAATSA LLLRLFTIGG VQKEVFSLAG PVVSMAGHGE QLFIVYHRGT GFDGDQCLGV QLLELGK KK KQILHGDPLP LTRKSYLAWI GFSAEGTPCY VDSEGIVRML NRGLGNTWTP ICNTREHCKG KSDHYWVVGI HENPQQLR C IPCKGSRFPP TLPRPAVAIL SFKLPYCQIA TEKGQMEEQF WRSVIFHNHL DYLAKNGYEY EESTKNQATK EQQELLMKM LALSCKLERE FRCVELADLM TQNAVNLAIK YASRSRKLIL AQKLSELAVE KAAELTATQV EEEEEEEDFR KKLNAGYSNT ATEWSQPRF RNQVEEDAED SGEADDEEKP EIHKPGQNSF SKSTNSSDVS AKSGAVTFSS QGRVNPFKVS ASSKEPAMSM N SARSTNIL DNMGKSSKKS TALSRTTNNE KSPIIKPLIP KPKPKQASAA SYFQKRNSQT NKTEEVKEEN LKNVLSETPA IC PPQNTEN QRPKTGFQMW LEENRSNILS DNPDFSDEAD IIKEGMIRFR VLSTEERKVW ANKAKGETAS EGTEAKKRKR VVD ESDETE NQEEKAKENL NLSKKQKPLD FSTNQKLSAF AFKQE

UniProtKB: WD repeat and HMG-box DNA-binding protein 1

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Macromolecule #15: Protein timeless homolog

MacromoleculeName: Protein timeless homolog / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 138.903031 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: GMDLHMMNCE LLATCSALGY LEGDTYHKEP DCLESVKDLI RYLRHEDETR DVRQQLGAAQ ILQSDLLPIL TQHHQDKPLF DAVIRLMVN LTQPALLCFG NLPKEPSFRH HFLQVLTYLQ AYKEAFASEK AFGVLSETLY ELLQLGWEER QEEDNLLIER I LLLVRNIL ...String:
GMDLHMMNCE LLATCSALGY LEGDTYHKEP DCLESVKDLI RYLRHEDETR DVRQQLGAAQ ILQSDLLPIL TQHHQDKPLF DAVIRLMVN LTQPALLCFG NLPKEPSFRH HFLQVLTYLQ AYKEAFASEK AFGVLSETLY ELLQLGWEER QEEDNLLIER I LLLVRNIL HVPADLDQEK KIDDDASAHD QLLWAIHLSG LDDLLLFLAS SSAEEQWSLH VLEIVSLMFR DQNPEQLAGV GQ GRLAQER SADFAELEVL RQREMAEKKT RALQRGNRHS RFGGSYIVQG LKSIGERDLI FHKGLHNLRN YSSDLGKQPK KVP KRRQAA RELSIQRRSA LNVRLFLRDF CSEFLENCYN RLMGSVKDHL LREKAQQHDE TYYMWALAFF MAFNRAASFR PGLV SETLS VRTFHFIEQN LTNYYEMMLT DRKEAASWAR RMHLALKAYQ ELLATVNEMD ISPDEAVRES SRIIKNNIFY VMEYR ELFL ALFRKFDERC QPRSFLRDLV ETTHLFLKML ERFCRSRGNL VVQNKQKKRR KKKKKVLDQA IVSGNVPSSP EEVEAV WPA LAEQLQCCAQ NSELSMDSVV PFDAASEVPV EEQRAEAMVR IQDCLLAGQA PQALTLLRSA REVWPEGDVF GSQDISP EE EIQLLKQILS APLPRQQGPE ERGAEEEEEE EEEEEEELQV VQVSEKEFNF LDYLKRFACS TVVRAYVLLL RSYQQNSA H TNHCIVKMLH RLAHDLKMEA LLFQLSVFCL FNRLLSDPAA GAYKELVTFA KYILGKFFAL AAVNQKAFVE LLFWKNTAV VREMTEGYGS LDDRSSSRRA PTWSPEEEAH LRELYLANKD VEGQDVVEAI LAHLNTVPRT RKQIIHHLVQ MGLADSVKDF QRKGTHIVL WTGDQELELQ RLFEEFRDSD DVLGHIMKNI TAKRSRARIV DKLLALGLVA ERRELYKKRQ KKLASSILPN G AESLKDFC QEDLEEEENL PEEDSEEEEE GGSEAEQVQG SLVLSNENLG QSLHQEGFSI PLLWLQNCLI RAADDREEDG CS QAVPLVP LTEENEEAME NEQFQQLLRK LGVRPPASGQ ETFWRIPAKL SPTQLRRAAA SLSQPEEEQK LQPELQPKVP GEQ GSDEEH CKEHRAQALR ALLLAHKKKA GLASPEEEDA VGKEPLKAAP KKRQLLDSDE EQEEDEGRNR APELGAPGIQ KKKR YQIED DEDD

UniProtKB: Protein timeless homolog

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Macromolecule #16: TIMELESS-interacting protein

MacromoleculeName: TIMELESS-interacting protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 34.600223 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MLEPQENGVI DLPDYEHVED ETFPPFPPPA SPERQDGEGT EPDEESGNGA PVRVPPKRTV KRNIPKLDAQ RLISERGLPA LRHVFDKAK FKGKGHEAED LKMLIRHMEH WAHRLFPKLQ FEDFIDRVEY LGSKKEVQTC LKRIRLDLPI LHEDFVSNND E VAENNEHD ...String:
MLEPQENGVI DLPDYEHVED ETFPPFPPPA SPERQDGEGT EPDEESGNGA PVRVPPKRTV KRNIPKLDAQ RLISERGLPA LRHVFDKAK FKGKGHEAED LKMLIRHMEH WAHRLFPKLQ FEDFIDRVEY LGSKKEVQTC LKRIRLDLPI LHEDFVSNND E VAENNEHD VTSTELDPFL TNLSESEMFA SELSRSLTEE QQQRIERNKQ LALERRQAKL LSNSQTLGND MLMNTPRAHT VE EVNTDED QKEESNGLNE DILDNPCNDA IANTLNEEET LLDQSFKNVQ QQLDATSRNI TEAR

UniProtKB: TIMELESS-interacting protein

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Macromolecule #19: Leucine-rich repeat protein 1

MacromoleculeName: Leucine-rich repeat protein 1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 46.789648 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MKLHCEVEVI SRHLPALGLR NRGKGVRAVL SLCQQTSRSQ PPVRAFLLIS TLKDKRGTRY ELRENIEQFF TKFVDEGKAT VRLKEPPVD ICLSKAISSS LKGFLSAMRL AHRGCNVDTP VSTLTPVKTS EFENFKTKMV ITSKKDYPLS KNFPYSLEHL Q TSYCGLVR ...String:
MKLHCEVEVI SRHLPALGLR NRGKGVRAVL SLCQQTSRSQ PPVRAFLLIS TLKDKRGTRY ELRENIEQFF TKFVDEGKAT VRLKEPPVD ICLSKAISSS LKGFLSAMRL AHRGCNVDTP VSTLTPVKTS EFENFKTKMV ITSKKDYPLS KNFPYSLEHL Q TSYCGLVR VDMRMLCLKS LRKLDLSHNH IKKLPATIGD LIHLQELNLN DNHLESFSVA LCHSTLQKSL RSLDLSKNKI KA LPVQFCQ LQELKNLKLD DNELIQFPCK IGQLINLRFL SAARNKLPFL PSEFRNLSLE YLDLFGNTFE QPKVLPVIKL QAP LTLLES SARTILHNRI PYGSHIIPFH LCQDLDTAKI CVCGRFCLNS FIQGTTTMNL HSVAHTVVLV DNLGGTEAPI ISYF CSLGC YVNSSDMLK

UniProtKB: Leucine-rich repeat protein 1

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Macromolecule #20: Elongin-B

MacromoleculeName: Elongin-B / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.147781 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString:
MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALC IEPFSSPPEL PDVMKPQDSG SSANEQAVQ

UniProtKB: Elongin-B

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Macromolecule #21: Claspin

MacromoleculeName: Claspin / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 155.184703 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MTGEVGSEVH LEINDPNVIS QEEADSPSDS GQGSYETIGP LSEGDSDEEI FVSKKLKNRK VLQDSDSETE DTNASPEKTT YDSAEEENK ENLYAGKNTK IKRIYKTVAD SDESYMEKSL YQENLEAQVK PCLELSLQSG NSTDFTTDRK SSKKHIHDKE G TAGKAKVK ...String:
MTGEVGSEVH LEINDPNVIS QEEADSPSDS GQGSYETIGP LSEGDSDEEI FVSKKLKNRK VLQDSDSETE DTNASPEKTT YDSAEEENK ENLYAGKNTK IKRIYKTVAD SDESYMEKSL YQENLEAQVK PCLELSLQSG NSTDFTTDRK SSKKHIHDKE G TAGKAKVK SKRRLEKEER KMEKIRQLKK KETKNQEDDV EQPFNDSGCL LVDKDLFETG LEDENNSPLE DEESLESIRA AV KNKVKKH KKKEPSLESG VHSFEEGSEL SKGTTRKERK AARLSKEALK QLHSETQRLI RESALNLPYH MPENKTIHDF FKR KPRPTC HGNAMALLKS SKYQSSHHKE IIDTANTTEM NSDHHSKGSE QTTGAENEVE TNALPVVSKE TQIITGSDES CRKD LVKNE ELEIQEKQKQ SDIRPSPGDS SVLQQESNFL GNNHSEECQV GGLVAFEPHA LEGEGPQNPE ETDEKVEEPE QQNKS SAVG PPEKVRRFTL DRLKQLGVDV SIKPRLGADE DSFVILEPET NRELEALKQR FWKHANPAAK PRAGQTVNVN VIVKDM GTD GKEELKADVV PVTLAPKKLD GASHTKPGEK LQVLKAKLQE AMKLRRFEER QKRQALFKLD NEDGFEEEEE EEEEMTD ES EEDGEEKVEK EEKEEELEEE EEKEEEEEEE GNQETAEFLL SSEEIETKDE KEMDKENNDG SSEIGKAVGF LSVPKSLS S DSTLLLFKDS SSKMGYFPTE EKSETDENSG KQPSKLDEDD SCSLLTKESS HNSSFELIGS TIPSYQPCNR QTGRGTSFF PTAGGFRSPS PGLFRASLVS SASKSSGKLS EPSLPIEDSQ DLYNASPEPK TLFLGAGDFQ FCLEDDTQSQ LLDADGFLNV RNHRNQYQA LKPRLPLASM DENAMDANMD ELLDLCTGKF TSQAEKHLPR KSDKKENMEE LLNLCSGKFT SQDASTPASS E LNKQEKES SMGDPMEEAL ALCSGSFPTD KEEEDEEEEF GDFRLVSNDN EFDSDEDEHS DSGNDLALED HEDDDEEELL KR SEKLKRQ MRLRKYLEDE AEVSGSDVGS EDEYDGEEID EYEEDVIDEV LPSDEELQSQ IKKIHMKTML DDDKRQLRLY QER YLADGD LHSDGPGRMR KFRWKNIDDA SQMDLFHRDS DDDQTEEQLD ESEARWRKER IEREQWLRDM AQQGKITAEE EEEI GEDSQ FMILAKKVTA KALQKNASRP MVIQESKSLL RNPFEAIRPG SAQQVKTGSL LNQPKAVLQK LAALSDHNPS APRNS RNFV FHTLSPVKAE AAKESSKSQV KKRGPSFMTS PSPKHLKTDD STSGLTRSIF KYLESLEVLF QGPDYKDDDD KDYKDD DDK DYKDDDDK

UniProtKB: Claspin

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Macromolecule #22: Elongin-C

MacromoleculeName: Elongin-C / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 12.485135 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString:
MDGEEKTYGG CEGPDAMYVK LISSDGHEFI VKREHALTSG TIKAMLSGPG QFAENETNEV NFREIPSHVL SKVCMYFTYK VRYTNSSTE IPEFPIAPEI ALELLMAANF LDC

UniProtKB: Elongin-C

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Macromolecule #23: Cullin-2

MacromoleculeName: Cullin-2 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 87.09893 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSLKPRVVDF DETWNKLLTT IKAVVMLEYV ERATWNDRFS DIYALCVAYP EPLGERLYTE TKIFLENHVR HLHKRVLESE EQVLVMYHR YWEEYSKGAD YMDCLYRYLN TQFIKKNKLT EADLQYGYGG VDMNEPLMEI GELALDMWRK LMVEPLQAIL I RMLLREIK ...String:
MSLKPRVVDF DETWNKLLTT IKAVVMLEYV ERATWNDRFS DIYALCVAYP EPLGERLYTE TKIFLENHVR HLHKRVLESE EQVLVMYHR YWEEYSKGAD YMDCLYRYLN TQFIKKNKLT EADLQYGYGG VDMNEPLMEI GELALDMWRK LMVEPLQAIL I RMLLREIK NDRGGEDPNQ KVIHGVINSF VHVEQYKKKF PLKFYQEIFE SPFLTETGEY YKQEASNLLQ ESNCSQYMEK VL GRLKDEE IRCRKYLHPS SYTKVIHECQ QRMVADHLQF LHAECHNIIR QEKKNDMANM YVLLRAVSTG LPHMIQELQN HIH DEGLRA TSNLTQENMP TLFVESVLEV HGKFVQLINT VLNGDQHFMS ALDKALTSVV NYREPKSVCK APELLAKYCD NLLK KSAKG MTENEVEDRL TSFITVFKYI DDKDVFQKFY ARMLAKRLIH GLSMSMDSEE AMINKLKQAC GYEFTSKLHR MYTDM SVSA DLNNKFNNFI KNQDTVIDLG ISFQIYVLQA GAWPLTQAPS STFAIPQELE KSVQMFELFY SQHFSGRKLT WLHYLC TGE VKMNYLGKPY VAMVTTYQMA VLLAFNNSET VSYKELQDST QMNEKELTKT IKSLLDVKMI NHDSEKEDID AESSFSL NM NFSSKRTKFK ITTSMQKDTP QEMEQTRSAV DEDRKMYLQA AIVRIMKARK VLRHNALIQE VISQSRARFN PSISMIKK C IEVLIDKQYI ERSQASADEY SYVA

UniProtKB: Cullin-2

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Macromolecule #24: E3 ubiquitin-protein ligase RBX1

MacromoleculeName: E3 ubiquitin-protein ligase RBX1 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 12.289977 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString:
MAAAMDVDTP SGTNSGAGKK RFEVKKWNAV ALWAWDIVVD NCAICRNHIM DLCIECQANQ ASATSEECTV AWGVCNHAFH FHCISRWLK TRQVCPLDNR EWEFQKYGH

UniProtKB: E3 ubiquitin-protein ligase RBX1

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Macromolecule #17: Leading strand DNA

MacromoleculeName: Leading strand DNA / type: dna / ID: 17 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 26.396836 KDa
SequenceString: (DT)(DA)(DG)(DA)(DG)(DT)(DA)(DG)(DG)(DA) (DA)(DG)(DT)(DG)(DA)(DT)(DG)(DG)(DT)(DA) (DA)(DG)(DT)(DG)(DA)(DT)(DT)(DA)(DG) (DA)(DG)(DA)(DA)(DT)(DT)(DG)(DG)(DA)(DG) (DA) (DG)(DT)(DG)(DT)(DG)(DT) ...String:
(DT)(DA)(DG)(DA)(DG)(DT)(DA)(DG)(DG)(DA) (DA)(DG)(DT)(DG)(DA)(DT)(DG)(DG)(DT)(DA) (DA)(DG)(DT)(DG)(DA)(DT)(DT)(DA)(DG) (DA)(DG)(DA)(DA)(DT)(DT)(DG)(DG)(DA)(DG) (DA) (DG)(DT)(DG)(DT)(DG)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)

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Macromolecule #18: Lagging strand DNA

MacromoleculeName: Lagging strand DNA / type: dna / ID: 18 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 12.279929 KDa
SequenceString:
(DA)(DC)(DA)(DC)(DA)(DC)(DA)(DC)(DT)(DC) (DT)(DC)(DC)(DA)(DA)(DT)(DT)(DC)(DT)(DC) (DT)(DA)(DA)(DT)(DC)(DA)(DC)(DT)(DT) (DA)(DC)(DC)(DA)(DT)(DC)(DA)(DC)(DT)(DT) (DC) (DC)

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Macromolecule #25: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 25 / Number of copies: 8 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #26: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 26 / Number of copies: 3 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #27: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / type: ligand / ID: 27 / Number of copies: 3 / Formula: ANP
Molecular weightTheoretical: 506.196 Da
Chemical component information

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

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Macromolecule #28: SULFATE ION

MacromoleculeName: SULFATE ION / type: ligand / ID: 28 / Number of copies: 1 / Formula: SO4
Molecular weightTheoretical: 96.063 Da
Chemical component information

ChemComp-SO4:
SULFATE ION

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number real images: 16721 / Average exposure time: 4.0 sec. / Average electron dose: 38.8 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 2.7 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 0.1 mm / Nominal defocus max: 2.8000000000000003 µm / Nominal defocus min: 0.8 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 2412000
Startup modelType of model: OTHER
Final reconstructionNumber classes used: 1 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 2) / Number images used: 232000
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 3)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2)
FSC plot (resolution estimation)

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