- EMDB-12256: Structure of the autoinducer-2 exporter TqsA from E. coli -
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基本情報
登録情報
データベース: EMDB / ID: EMD-12256
タイトル
Structure of the autoinducer-2 exporter TqsA from E. coli
マップデータ
C5 symmetry imposed final map for TqsA
試料
複合体: Pentameric TqsA
タンパク質・ペプチド: AI-2 transport protein TqsA
キーワード
cryo-EM / membrane protein / autoinducer-2 exporter / quorum sensing / pentamer / protein oligomerization / STRUCTURAL PROTEIN
機能・相同性
autoinducer AI-2 transmembrane transport / Transmembrane protein TqsA-like / AI-2E family transporter / quorum sensing / plasma membrane => GO:0005886 / efflux transmembrane transporter activity / transmembrane transport / AI-2 transport protein TqsA
ジャーナル: EMBO J / 年: 2022 タイトル: Cryo-EM structures of pentameric autoinducer-2 exporter from Escherichia coli reveal its transport mechanism. 著者: Radhika Khera / Ahmad R Mehdipour / Jani R Bolla / Joerg Kahnt / Sonja Welsch / Ulrich Ermler / Cornelia Muenke / Carol V Robinson / Gerhard Hummer / Hao Xie / Hartmut Michel / 要旨: Bacteria utilize small extracellular molecules to communicate in order to collectively coordinate their behaviors in response to the population density. Autoinducer-2 (AI-2), a universal molecule for ...Bacteria utilize small extracellular molecules to communicate in order to collectively coordinate their behaviors in response to the population density. Autoinducer-2 (AI-2), a universal molecule for both intra- and inter-species communication, is involved in the regulation of biofilm formation, virulence, motility, chemotaxis, and antibiotic resistance. While many studies have been devoted to understanding the biosynthesis and sensing of AI-2, very little information is available on its export. The protein TqsA from Escherichia coli, which belongs to the AI-2 exporter superfamily, has been shown to export AI-2. Here, we report the cryogenic electron microscopic structures of two AI-2 exporters (TqsA and YdiK) from E. coli at 3.35 Å and 2.80 Å resolutions, respectively. Our structures suggest that the AI-2 exporter exists as a homo-pentameric complex. In silico molecular docking and native mass spectrometry experiments were employed to demonstrate the interaction between AI-2 and TqsA, and the results highlight the functional importance of two helical hairpins in substrate binding. We propose that each monomer works as an independent functional unit utilizing an elevator-type transport mechanism.