ジャーナル: Proc Natl Acad Sci U S A / 年: 2021 タイトル: The effect of the D614G substitution on the structure of the spike glycoprotein of SARS-CoV-2. 著者: Donald J Benton / Antoni G Wrobel / Chloë Roustan / Annabel Borg / Pengqi Xu / Stephen R Martin / Peter B Rosenthal / John J Skehel / Steven J Gamblin / 要旨: The majority of currently circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses have mutant spike glycoproteins that contain the D614G substitution. Several studies have ...The majority of currently circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses have mutant spike glycoproteins that contain the D614G substitution. Several studies have suggested that spikes with this substitution are associated with higher virus infectivity. We use cryo-electron microscopy to compare G614 and D614 spikes and show that the G614 mutant spike adopts a range of more open conformations that may facilitate binding to the SARS-CoV-2 receptor, ACE2, and the subsequent structural rearrangements required for viral membrane fusion.
EMPIAR-10725 (タイトル: Single particle cryo EM dataset of SARS-CoV-2 Spike protein with D614G substitution Data size: 9.8 TB Data #1: Unaligned multi-frame micrographs of SARS-CoV-2 Spike with D614G substitution [micrographs - multiframe])