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- EMDB-0289: Structure of a functional obligate respiratory supercomplex from ... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-0289 | |||||||||
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Title | Structure of a functional obligate respiratory supercomplex from Mycobacterium smegmatis | |||||||||
![]() | Respiratory supercomplex from Mycobacterium smegmatis | |||||||||
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Function / homology | ![]() aerobic electron transport chain / cytochrome-c oxidase / oxidative phosphorylation / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / cytochrome-c oxidase activity / electron transport coupled proton transport / : / ATP synthesis coupled electron transport / respiratory electron transport chain ...aerobic electron transport chain / cytochrome-c oxidase / oxidative phosphorylation / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / cytochrome-c oxidase activity / electron transport coupled proton transport / : / ATP synthesis coupled electron transport / respiratory electron transport chain / electron transport chain / aerobic respiration / 2 iron, 2 sulfur cluster binding / membrane => GO:0016020 / oxidoreductase activity / iron ion binding / copper ion binding / heme binding / membrane / metal ion binding / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.3 Å | |||||||||
![]() | Wiseman B / Nitharwal RG / Fedotovskaya O / Schafer J / Guo H / Kuang Q / Benlekbir S / Sjostrand D / Adelroth P / Rubinstein JL ...Wiseman B / Nitharwal RG / Fedotovskaya O / Schafer J / Guo H / Kuang Q / Benlekbir S / Sjostrand D / Adelroth P / Rubinstein JL / Brzezinski P / Hogbom M | |||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Structure of a functional obligate complex IIIIV respiratory supercomplex from Mycobacterium smegmatis. Authors: Benjamin Wiseman / Ram Gopal Nitharwal / Olga Fedotovskaya / Jacob Schäfer / Hui Guo / Qie Kuang / Samir Benlekbir / Dan Sjöstrand / Pia Ädelroth / John L Rubinstein / Peter Brzezinski / Martin Högbom / ![]() ![]() ![]() Abstract: In the mycobacterial electron-transport chain, respiratory complex III passes electrons from menaquinol to complex IV, which in turn reduces oxygen, the terminal acceptor. Electron transfer is ...In the mycobacterial electron-transport chain, respiratory complex III passes electrons from menaquinol to complex IV, which in turn reduces oxygen, the terminal acceptor. Electron transfer is coupled to transmembrane proton translocation, thus establishing the electrochemical proton gradient that drives ATP synthesis. We isolated, biochemically characterized, and determined the structure of the obligate IIIIV supercomplex from Mycobacterium smegmatis, a model for Mycobacterium tuberculosis. The supercomplex has quinol:O oxidoreductase activity without exogenous cytochrome c and includes a superoxide dismutase subunit that may detoxify reactive oxygen species produced during respiration. We found menaquinone bound in both the Q and Q sites of complex III. The complex III-intrinsic diheme cytochrome cc subunit, which functionally replaces both cytochrome c and soluble cytochrome c in canonical electron-transport chains, displays two conformations: one in which it provides a direct electronic link to complex IV and another in which it serves as an electrical switch interrupting the connection. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 10.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 29.5 KB 29.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 15.8 KB | Display | ![]() |
Images | ![]() | 59.6 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6hwhMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Respiratory supercomplex from Mycobacterium smegmatis | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
+Entire : Respiratory Supercomplex from Mycobacterium smegmatis
+Supramolecule #1: Respiratory Supercomplex from Mycobacterium smegmatis
+Macromolecule #1: Ubiquinol-cytochrome c reductase iron-sulfur subunit
+Macromolecule #2: Co-purified unknown transmembrane helices built as polyALA
+Macromolecule #3: Co-purified unknown transmembrane helices built as polyALA
+Macromolecule #4: Co-purified unknown peptide built as polyALA
+Macromolecule #5: Co-purified unknown peptide built as polyALA
+Macromolecule #6: Cytochrome bc1 complex cytochrome c subunit
+Macromolecule #7: Cytochrome c oxidase subunit 2
+Macromolecule #8: MSMEG_4693
+Macromolecule #9: Uncharacterized protein MSMEG_4692/MSMEI_4575
+Macromolecule #10: Cytochrome c oxidase subunit 1
+Macromolecule #11: Cytochrome c oxidase polypeptide 4
+Macromolecule #12: Cytochrome c oxidase subunit 3
+Macromolecule #13: Ubiquinol-cytochrome C reductase QcrB
+Macromolecule #14: FE2/S2 (INORGANIC) CLUSTER
+Macromolecule #15: CARDIOLIPIN
+Macromolecule #16: MENAQUINONE-9
+Macromolecule #17: COPPER (II) ION
+Macromolecule #18: HEME-AS
+Macromolecule #19: HEME C
+Macromolecule #20: PROTOPORPHYRIN IX CONTAINING FE
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 5 mg/mL |
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Buffer | pH: 7.5 |
Grid | Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR / Details: 40 seconds at 20 mA |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: COUNTING / Number grids imaged: 1 / Number real images: 5316 / Average exposure time: 60.0 sec. / Average electron dose: 43.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Details | Model was built de novo; except for chains i,j which are polyALA homology models rigid-body docked into low resolution density. |
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Refinement | Space: REAL / Protocol: OTHER / Target criteria: Cross-correlation coefficient |
Output model | ![]() PDB-6hwh: |