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TitleRapid and Label-Free Structural Proteomics Using One-Step Swift Trypsin LiP-MS.
Journal, issue, pagesACS Omega, Vol. 11, Issue 1, Page 2152-2162, Year 2026
Publish dateJan 13, 2026
AuthorsYasuomi Miyashita / Ryo Konno / Satoshi Ogasawara / Yusei Okuda / Yuuki Takamuku / Toshio Moriya / Tetsuichiro Saito / Takeshi Murata / Osamu Ohara / Yusuke Kawashima /
PubMed AbstractLimited proteolysis mass spectrometry (LiP-MS) is a powerful approach for probing protein conformational changes on a proteome-wide scale. However, conventional workflows rely on a two-step digestion ...Limited proteolysis mass spectrometry (LiP-MS) is a powerful approach for probing protein conformational changes on a proteome-wide scale. However, conventional workflows rely on a two-step digestion with proteinase K and trypsin, which increases complexity and reduces reproducibility and sensitivity. This study aimed to develop a simplified one-step protocol, termed Swift Trypsin LiP-MS (STLiP-MS), which uses a trypsin-immobilized spin column and high-speed centrifugation to achieve rapid and reproducible surface-limited proteolysis. Using HEK293 cell extracts, STLiP-MS identified 286 proteins exhibiting conformational changes upon phosphatase inhibition, including 37 enriched in phosphatase-related Gene Ontology categories. The method improvements, including suppression of predigestion and immediate enzyme inactivation, further increased sensitivity, enabling the detection of 799 proteins with structural alterations, of which 77 were enriched in phosphatase-related categories. Comparison with the single-pot solid-phase-enhanced sample preparation (SP3) method confirmed that these changes originated from structure-selective proteolysis and were not detectable under fully denaturing conditions. To demonstrate its broader applicability, we applied STLiP-MS to the adenosine A receptor (A-BRIL) and observed antibody-induced protection of extracellular loop 2 (residues 147-176). Cryogenic electron microscopy validated Fab fragment binding to the same region, confirming the correspondence between STLiP-MS signals and actual antibody-antigen interfaces. Collectively, these results show that STLiP-MS is a rapid and robust platform that enables sensitive, label-free detection of local structural changes under near-physiological conditions and accurate prediction of protein-protein interaction sites. This method holds great promise for applications in structural proteomics and drug target identification.
External linksACS Omega / PubMed:41552556 / PubMed Central
MethodsEM (single particle)
Resolution3.45 Å
Structure data

67107

9xqb

EMDB-67107, PDB-9xqb:
Cryo-EM structure of the human A2A adenosine receptor in complex with a Fab antibody fragment
Method: EM (single particle) / Resolution: 3.45 Å

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
KeywordsMEMBRANE PROTEIN / Cryo-EM Lip-MS

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