Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structures and inhibition of the Crimean-Congo haemorrhagic fever virus polymerase.
Journal, issue, pages	Nature, Year 2026
Publish date	Jul 22, 2026
Authors	Lu Xue / Jiacheng Gui / Hainei Pan / Fan Wu / Shenghua Gao / Wenhua Kuang / Tiancai Chang / Zimu Li / Binqian Zou / Heyu Zhao / Mei Li / Min Zhou / Hongyu Yuan / Lijun Rong / Peng Gong / Jun He / Zengqin Deng / Manli Wang / Peng Zhan / Xinwen Chen / Xiaoli Xiong /
PubMed Abstract	Crimean-Congo haemorrhagic fever virus (CCHFV) is a tick-borne virus and causes severe, often fatal, human infections. Lacking licensed vaccines or drugs, CCHFV is a World Health Organization ...Crimean-Congo haemorrhagic fever virus (CCHFV) is a tick-borne virus and causes severe, often fatal, human infections. Lacking licensed vaccines or drugs, CCHFV is a World Health Organization priority pathogen requiring urgent development of medical countermeasures. The CCHFV Large (L) protein functions as the viral RNA-dependent RNA polymerase CCHFV-L, representing a promising antiviral target, and is among the largest viral polymerases in the order Bunyavirales. Here we define the cofactors required for CCHFV-L RNA synthesis in vitro, enabling capture and determination of elongating CCHFV-L-RNA complex structures. The structures show a markedly enlarged polymerase architecture, revealing that CCHFV-L RNA synthesis is accompanied by ordering of the polymerase peripheral domains. We also define how the baloxavir-derived experimental drug WXSH0208 (ref. ) and the nucleoside analogue 2'-deoxy-2'-fluorocytidine, which has nanomolar cellular potency, inhibit this polymerase through endonuclease inhibition and post-translocation chain termination, respectively. Together, these results should structurally guide rational optimization of inhibitors directed against CCHFV-L.
External links	Nature / PubMed:42486991
Methods	EM (single particle) / X-ray diffraction
Resolution	1.85 - 3.09 Å
Structure data	66754 9xd4 EMDB-66754, PDB-9xd4: Crimean-Congo hemorrhagic fever virus RNA polymerase Method: EM (single particle) / Resolution: 2.79 Å 66755 9xd5 EMDB-66755, PDB-9xd5: Crimean-Congo hemorrhagic fever virus RNA polymerase in complex with the 5' vRNA Method: EM (single particle) / Resolution: 2.72 Å 66756 9xd6 EMDB-66756, PDB-9xd6: Crimean-Congo hemorrhagic fever virus RNA polymerase in complex with the 3' vRNA Method: EM (single particle) / Resolution: 2.74 Å 66783 9xe6 EMDB-66783, PDB-9xe6: Crimean-Congo hemorrhagic fever virus RNA polymerase containing a 10-bp RNA product and incorporated 2FC Method: EM (single particle) / Resolution: 3.09 Å 66784 9xe7 EMDB-66784, PDB-9xe7: Crimean-Congo hemorrhagic fever virus RNA polymerase containing a 10-bp RNA product and incorporated cytidine Method: EM (single particle) / Resolution: 3.02 Å 66785 9xe9 EMDB-66785, PDB-9xe9: Crimean-Congo hemorrhagic fever virus RNA polymerase containing a 15-bp RNA product Method: EM (single particle) / Resolution: 2.81 Å 66787 9xec EMDB-66787, PDB-9xec: Crimean-Congo hemorrhagic fever virus RNA polymerase containing a 9-bp RNA product Method: EM (single particle) / Resolution: 2.98 Å PDB-9xf9: Crystal structure of Kasokero virus cap- snatching endonuclease in complex with WXS Method: X-RAY DIFFRACTION / Resolution: 1.85 Å
Chemicals	ChemComp-ZN: Unknown entry ChemComp-2KH: 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine ChemComp-MN: Unknown entry PDB-1ezw: STRUCTURE OF COENZYME F420 DEPENDENT TETRAHYDROMETHANOPTERIN REDUCTASE FROM METHANOPYRUS KANDLERI ChemComp-HOH: WATER
Source	crimean-congo hemorrhagic fever virus strain ibar10200 kasokero virus mus musculus (house mouse)
Keywords	VIRAL PROTEIN / RNA polymerase / VIRUS / VIRAL PROTEIN/RNA / VIRAL PROTEIN-RNA complex / HYDROLASE / cap-snatching endonuclease / inhibitor